Construction of CD163-knockout induced pluripotent stem cells using reprogramming technology.

Purpose This study draws on value congruence theory and epistemic motivation theory to examine whether the effect of ethical and environmental appeals on consumers’ perceptions of technological improvement and support for government initiatives varies across different novel food technologies, including cellular agriculture, gene editing and controlled environment agriculture. It further investigates whether epistemic value acts as a psychological mechanism that mediates these effects. Design/methodology/approach A 3 (appeal: ethical, environmental and control) × 3 (food technology: cellular agriculture, gene editing and controlled environment agriculture) between-subjects experimental design was employed to assess the effectiveness of strategic communication in enhancing consumer perceptions. Mediation analyses tested whether epistemic value explains the relationship between these relationships. Findings Based on data from 1,360 New Zealand consumers, ethical and environmental appeals increased support for cellular agriculture but not for gene editing or controlled environment agriculture, likely due to differences in how consumers perceive these technologies relative to the message appeals. Additionally, epistemic value emerged as a key psychological mechanism that helps explain these differential effects. Originality/value This study highlights the importance of aligning message appeals with consumers’ perceptions of novel food technologies. It provides both theoretical and practical insights for improving public support for novel food technologies through more targeted and aligned communication strategies.

SpCas9-mediated gene editing in bovine embryo via single adeno-associated virus infection using a novel micro-sized promoter.

Emerging trends in the development of efficient CAS nucleases for meticulous gene editing in plants.

Neutrophils are one of the key components of the innate immune system, and they play essential roles in various physiological processes, including phagocytosis, chemotaxis, immune sensing, and transmigration across the vascular endothelium. The synergy of neutrophil biology with nanomaterial science has led to the development of innovative neutrophil-based drug delivery systems (NDDSs) or neutrophil-derived biomimetic delivery systems. In this review, we elucidate the mechanisms underlying neutrophil-mediated targeting strategies. By utilizing inherent properties of neutrophils, targeted delivery to specific disease sites through NDDSs can be achieved. We survey various approaches for constructing NDDSs via live cell delivery strategies involving cell loading, in vivo capture, surface modification, and gene editing, as well as neutrophil-mimicking approaches based on neutrophil membranes, exosomes, and neutrophil-like cells. Manipulation of drug loading and release from NDDSs and functionalization of neutrophils allow for precise regulation and intervention of disease procession. In addition, we propose emerging approaches for novel NDDSs from an immunometabolic perspective. Finally, we address challenges and opportunities for advancing NDDSs into clinical practice.

Interplay and integrated regulation of astaxanthin-lipid biosynthesis in microbial systems: A review from metabolic pathway cross-talk to biotechnological optimization.

Can myostatin editing together with gut microbiota modulation produce more and tastier meat?

Comprehensive evaluation of the breeding value of OsPUP7 in rice.

Nanoparticles-mediated intratumoral gene editing targeting PD-L1 and Galectin-9 for improved cancer immunotherapy.

Review of design strategies for active targeted drug delivery systems for pancreatic cancer.

CRISPR/anti-CRISPR genome editing in Clostridium beijerinckii.

Establishment and application of efficient protoplast isolation and transformation system from leaves of multi-genotype poplars.

Current progress in the elucidation of Acute Myeloid Leukemia (AML) immune landscapes.

Advances in targeted therapeutics and smart delivery systems based on precision nano-oncology.

While extensively studied for its central role in tumor suppression (regulating cell cycle, DNA repair, and apoptosis), the p53 gene is now recognized for its significant involvement in musculoskeletal diseases. Aberrant p53 expression and function are key factors in the pathogenesis and progression, although the specific mechanisms and clinical change potential remain incompletely defined. This review systematically summarizes the multifaceted roles of p53 in major musculoskeletal diseases, analyzes the molecular mechanisms driving disease progression, and evaluates its potential as a therapeutic target. A comprehensive literature search was conducted in PubMed January 2008 to March 2025 using keywords including P53, osteoporosis, osteoarthritis, rheumatoid arthritis, gout, low back pain, and scoliosis. Original research, reviews, and clinical trials focusing on p53 mechanisms were included. 90 relevant articles were selected for analysis. p53 critically influences the development and progression of musculoskeletal diseases (osteoporosis, osteoarthritis, rheumatoid arthritis, low back pain, gout, and scoliosis) through diverse mechanisms: Disrupting bone formation/resorption balance (via the p53-Nedd4-Runx2 axis in osteoporosis). Promoting chondrocyte apoptosis (via the miR-34a-SIRT1-p53 pathway in osteoarthritis). Modulating inflammatory mediators (TNF-α, IL-6 in rheumatoid arthritis). Regulating oxidative stress responses (p53-SLC2A9 axis in gout). p53 exhibits dual roles (pro-apoptotic vs. anti-inflammatory), necessitating precise targeting strategies. Promising therapeutic interventions include p53-focused gene editing (CRISPR/Cas9), small-molecule inhibitors (PFT-α), and natural products (naringin). However, further clinical validation is essential. Future research requires multidisciplinary approaches to deepen understanding of p53 mechanisms and advance clinical applications in musculoskeletal disorders.

Methods for detecting off-target effects of CRISPR/Cas9.

From IscB to Cas9: Engineering and advances in the next generation of miniature gene editing tools.

From soil to biomanufacturing: Systems-driven metabolic pathway rewiring in non-model bacteria for gram-scale antibiotic production.

Pennycress (Thlaspi arvense) is a winter oilseed domesticated recently to be incorporated as an intermediate crop between the existing cropping systems of the US Midwest. We show that a natural accession of pennycress, 2032, is more susceptible to the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Alternaria japonica than the reference pennycress accession MN106. A previously identified marker associated with early flowering and maturity in pennycress was found to be present in a gene homologous to Arabidopsis Jumonji 14 (JMJ14). It has been reported that AtJMJ14 promotes disease resistance and represses flowering, and greenhouse studies of breeding populations suggested that this was also the case in pennycress. Plants with the 2032 TaJMJ14 allele were more susceptible to fungi and flowered early. CRISPR-Cas9 editing was used to generate additional TaJMJ14 alleles. A 9-base pair deletion in the sixth exon of TaJMJ14 showed trends of early flowering and S. sclerotiorum susceptibility, whereas a complete loss-of-function allele led to infertility. We further investigated the transcriptomes of MN106 and 2032 plants in the early stages of S. sclerotiorum and A. japonica infection to identify potential resistance and susceptibility genes. Differences in the expression of pathogen-associated molecular pattern-triggered immunity-associated genes led us to discover that 2032 plants have defects in elicitor-triggered oxidative bursts. The transcriptional responses unique to each accession lay a foundation for future gene editing and breeding approaches to keep the beneficial early flowering phenotype conferred by 2032 but uncouple it from disease susceptibility.

Emerging Targeted Therapies for Inherited Cardiomyopathies and Arrhythmias.