Evolutionary Mechanisms Research Articles

Genomic analysis and phylogenetic characterization of Himalayan snow trout, Schizothorax esocinus based on mitochondrial protein-coding genes.

Mitochondrial DNA (mtDNA) has become a significant tool for exploring genetic diversity and delineating evolutionary links across diverse taxa. Within the group of cold-water fish species that are native to the Indian Himalayan region, Schizothorax esocinus holds particular importance due to its ecological significance and is potentially vulnerable to environmental changes. This research aims to clarify the phylogenetic relationships within the Schizothorax genus by utilizing mitochondrial protein-coding genes. Standard protocols were followed for the isolation of DNA from S. esocinus. For the amplification of mtDNA, overlapping primers were used, and then subsequent sequencing was performed. The genetic features were investigated by the application of bioinformatic approaches. These approaches covered the evaluation of nucleotide composition, codon usage, selective pressure using nonsynonymous substitution /synonymous substitution (Ka/Ks) ratios, and phylogenetic analysis. The study specifically examined the 13 protein-coding genes of Schizothorax species which belongs to the Schizothoracinae subfamily. Nucleotide composition analysis showed a bias towards A + T content, consistent with other cyprinid fish species, suggesting evolutionary conservation. Relative Synonymous Codon Usage highlighted leucine as the most frequent (5.18%) and cysteine as the least frequent (0.78%) codon. The positive AT-skew and the predominantly negative GC-skew indicated the abundance of A and C. Comparative analysis revealed significant conservation of amino acids in multiple genes. The majority of amino acids were hydrophobic rather than polar. The purifying selection was revealed by the genetic distance and Ka/Ks ratios. Phylogenetic study revealed a significant genetic divergence between S. esocinus and other Schizothorax species with interspecific K2P distances ranging from 0.00 to 8.87%, with an average of 5.76%. The present study provides significant contributions to the understanding of mitochondrial genome diversity and genetic evolution mechanisms in Schizothoracinae, hence offering vital insights for the development of conservation initiatives aimed at protecting freshwater fish species.

Taking a Tailored Approach to Material Design: A Mechanistic Study of the Selective Localization of Phase-Separated Graphene Microdomains.

To achieve multifunctional properties using nanocomposites, selectively locating nanofillers in specific areas by tailoring a mixture of two immiscible polymers has been widely investigated. Forming a phase-separated structure from entirely miscible molecules is rarely reported, and the related mechanisms to govern the formation of assemblies from molecules have not been fully resolved. In this work, a novel method and the underlying mechanism to fabricate self-assembling, bicontinuous, biphasic structures with localized domains made up of amine-functionalized graphene nanoplatelets are presented, involving the tailoring of compositions in a liquid processable multicomponent epoxy blend. Kinetics studies were carried out to investigate the differences in reactivity of various epoxy-hardener pairs. Molecular dynamics simulations and in situ optical photothermal infrared spectroscopy measurements revealed the trajectories of different components during the early stages of polymerization, supporting the migration (phase behavior) of each component during the curing process. Confirmed by the phase structure and the correlated chemical maps down to the submicrometer level, it is believed that the bicontinuous phase separation is driven by the change of the miscibility between various building blocks forming during polymerization, leading to the formation of nanofiller domains. The proposed morphology evolution mechanism is based on combining solubility parameter calculations with kinetics studies, and preliminary experiments are performed to validate the applicability of the mechanism of selectively locating nanofillers in the phase-separated structure. This provides a simple yet sophisticated engineering model and a roadmap to a mechanism for fabricating phase-separated structures with nanofiller domains in nanocomposite films.

CLOCK evolved in cnidaria to synchronize internal rhythms with diel environmental cues.

The circadian clock enables anticipation of the day/night cycle in animals ranging from cnidarians to mammals. Circadian rhythms are generated through a transcription-translation feedback loop (TTFL or pacemaker) with CLOCK as a conserved positive factor in animals. However, CLOCK's functional evolutionary origin and mechanism of action in basal animals are unknown. In the cnidarian Nematostella vectensis, pacemaker gene transcript levels, including NvClk (the Clock ortholog), appear arrhythmic under constant darkness, questioning the role of NvCLK. Utilizing CRISPR/Cas9, we generated a NvClk allele mutant (NvClkΔ), revealing circadian behavior loss under constant dark (DD) or light (LL), while maintaining a 24 hr rhythm under light-dark condition (LD). Transcriptomics analysis revealed distinct rhythmic genes in wild-type (WT) polypsunder LD compared to DD conditions. In LD, NvClkΔ/Δ polyps exhibited comparable numbers of rhythmic genes, but were reduced in DD. Furthermore, under LD, the NvClkΔ/Δ polyps showed alterations in temporal pacemaker gene expression, impacting their potential interactions. Additionally, differential expression of non-rhythmic genes associated with cell division and neuronal differentiation was observed. These findings revealed that a light-responsive pathway can partially compensate for circadian clock disruption, and that the Clock gene has evolved in cnidarians to synchronize rhythmic physiology and behavior with the diel rhythm of the earth's biosphere.

Phased Telomere-to-Telomere Reference Genome and Pangenome Reveal an Expansion of Resistance Genes during Apple Domestication.

The cultivated apple (Malus domestica Borkh.) is a cross-pollinated perennial fruit tree of great economic importance. Previous versions of apple reference genomes were unphased, fragmented, and lacked comprehensive insights into the highly heterozygous genome, which impeded genetic studies and breeding programs in apple. In this study, we assembled a haplotype-resolved telomere-to-telomere reference genome for the diploid apple cultivar Golden Delicious. Subsequently, we constructed a pangenome based on twelve assemblies from wild and cultivated apples to investigate different types of resistance gene analogs (RGAs). Our results revealed the dynamics of the gene gain and loss events during apple domestication. Compared with cultivated species, more gene families in wild species were significantly enriched in oxidative phosphorylation, pentose metabolic process, responses to salt, and abscisic acid biosynthesis process. Interestingly, our analyses demonstrated a higher prevalence of RGAs in cultivated apples than their wild relatives, partially attributed to segmental and tandem duplication events in certain RGAs classes. Other types of structural variations, mainly deletions and insertions, have affected the presence and absence of TIR-NB-ARC-LRR (TNL), NB-ARC-LRR (NL), and CC-NB-ARC-LRR (CNL) genes. Additionally, hybridization/introgression from wild species has also contributed to the expansion of resistance genes in domesticated apples. Our haplotype-resolved T2T genome and pangenome provide important resources for genetic studies of apples, emphasizing the need to study the evolutionary mechanisms of resistance genes in apple breeding programs.

Mechanism of Electrocatalytic H2 Evolution, Carbonyl Hydrogenation, and Carbon-Carbon Coupling on Cu.

Aqueous-phase electrocatalytic hydrogenation of benzaldehyde on Cu leads not only to benzyl alcohol (the carbonyl hydrogenation product), but Cu also catalyzes carbon-carbon coupling to hydrobenzoin. In the absence of an organic substrate, H2 evolution proceeds via the Volmer-Tafel mechanism on Cu/C, with the Tafel step being rate-determining. In the presence of benzaldehyde, the catalyst surface is primarily covered with the organic substrate, while H* coverage is low. Mechanistically, the first H addition to the carbonyl O of an adsorbed benzaldehyde molecule leads to a surface-bound hydroxy intermediate. The hydroxy intermediate then undergoes a second and rate-determining H addition to its α-C to form benzyl alcohol. The H additions occur predominantly via the proton-coupled electron transfer mechanism. In a parallel reaction, the radical α-C of the hydroxy intermediate attacks the electrophilic carbonyl C of a physisorbed benzaldehyde molecule to form the C-C bond, which is rate-determining. The C-C coupling is accompanied by the protonation of the formed alkoxy radical intermediate, coupled with electron transfer from the surface of Cu, to form hydrobenzoin.

Engineering strategies for high‐voltage LiCoO2 based high‐energy Li‐ion batteries

AbstractTo drive electronic devices for a long range, the energy density of Li‐ion batteries must be further enhanced, and high‐energy cathode materials are required. Among the cathode materials, LiCoO2 (LCO) is one of the most promising candidates when charged to higher voltages over 4.3 V. However, high‐voltage LCO materials are confronted with severe surface and bulk issues inducing poor cyclic stability. To completely unleash the potential of LCO cathodes, a more comprehensive theoretical understanding of the underlying issues is necessary, along with active exploration of previous modifications. This paper mainly presents the degradation mechanisms of LCO under high voltage, the formation and evolution mechanisms of the cathode electrolyte interface, and the surface engineering strategies employed to enhance the cell performance. By organizing and summarizing these modifications, this work aims to establish associations among common research issues and to suggest future research priorities, thus facilitating the rapid development of high‐voltage LCO.

Study on the evolutionary mechanism of shrinkage stress-strain behavior of EVA-modified cement mortar at an early age

Understanding the early deformation characteristics is important to predict early cracks in cementitious materials and to serve structural engineering. Incorporation of polyethylene-vinyl acetate (EVA) dispersible polymer powder can improve the early cracking of cementitious materials. In this paper, the homogeneity, shrinkage stress-strain behavior and the evolutionary mechanism of EVA-modified cement mortar (EMCM) with different polymer-to-cement ratios (p/c) were investigated at an early age. The homogeneity and shrinkage stress-strain behavior of EMCM were characterized by a self-designed test setup. Isothermal calorimetric analysis, scanning electron microscopy and X-ray diffraction were used for the evolutionary mechanism analysis. The results show that the homogeneity of shrinkage of EMCM can be improved by adding EVA and prolonging the mixing time. The shrinkage stress of EMCM can be reduced by 83 % when p/c is 10 % and 15 %. Meanwhile, the elastic modulus of mortar can be reduced by 45 % when the EVA content is 15 %. Isothermal calorimetric analysis and characterization of microscopic properties show that EVA can inhibit both the generation and conversion of C4AH13, as well as the generation of AFt and CH, and retard the hydration of cement mortar. Within 3 h of hydration, the change in shrinkage stress of EMCM is only affected by the amount of AFt generated, while the change in shrinkage strain is affected by both CH and AFt generation, and the correlation with CH is greater. Finally, the interpenetrating network structure between the EVA and the cementitious matrix increases the interaction and bond between them, giving good deformability and thus reducing shrinkage. Therefore, the reduction in shrinkage stress-strain of EMCM is a result of the combined effect of the polymer on delaying cement hydration and increasing internal interaction and bonding.

Global and Local Ancestry and its Importance: A Mini-Review

Abstract: The fastest way to significantly change the composition of a population is through admixture, an evolutionary mechanism. In animal breeding history, genetic admixture has provided both short-term and long-term advantages by utilizing the phenomenon of complementarity and heterosis in several traits and genetic diversity, respectively. The traditional method of admixture analysis by pedigree records has now been replaced greatly by genome-wide marker data that enables more precise estimations. Among these markers, SNPs have been the popular choice since they are cost-effective, not so laborious, and automation of genotyping is easy. Certain markers can suggest the possibility of a population's origin from a sample of DNA where the source individual is unknown or unwilling to disclose their lineage, which are called Ancestry-Informative Markers (AIMs). Revealing admixture level at the locus-specific level is termed as local ancestry and can be exploited to identify signs of recent selective response and can account for genetic drift. Considering the importance of genetic admixture and local ancestry, in this mini-review, both concepts are illustrated, encompassing basics, their estimation/identification methods, tools/- software used and their applications.

Mechanism of formation and evolution of bound states in the continuum of split-hole all-dielectric metasurface under asymmetric displacement perturbation

Abstract Bound states in the continuum (BICs) with ultra-high Q properties have attracted much attention for their perfect localization in the continuous spectral range coexisting with extended waves. In this study, breaking the traditional excitation form of structure breakage or excitation field asymmetry, a monomeric silicon nanodisk array with relative displacement generated by the complete splitting of square nanopores is proposed based on the unique electromagnetic properties of all-dielectric metamaterials. During the introduction of perturbations by asymmetric displacements of splitting holes, it is shown by numerical simulations that two BICs at different wavelengths can be realized. Combined with eigenmodes of group theory, the symmetric matching relationship between the symmetry-protected BICs and the free-space radiation during the evolution process is analytically demonstrated, and the formation mechanism and the evolution law of the BICs excited by this metasurface are deeply investigated. meanwhile, it also provides a theoretical basis for the polarization dependence of quasi-BICs excitation and the ultra-high Q factor expression of BICs. Furthermore, near-field distribution and multipole decomposition show that the field distribution and surface currents support the excitation of BIC-driven toroidal dipole and magnetic quadrupole dual modes. This study not only provides an effective reference for the stability of high-Q resonance wavelengths, but also solves the problem of the lack of universality in analyzing the resonance mechanism based on resonance phenomena, and provides solid theoretical support for the study of displacement-mediated BICs resonance excitation and evolution.

Mechanism of Evolution of Shock Wave of Muzzle Jet under Initial Interference and Its Simplified Model

Large-caliber and long-barrel weapons are important experimental devices for exploring the impact resistance and reliability of warheads. The force of impact of the muzzle jet has a significant influence on the overload resistance of the warhead and surrounding devices. The mechanism of motion of the body inside the tube cannot be ignored owing to the high kinetic energy at the muzzle. In this study, we designed the relevant experiment and a simulation model to analyze the structural characteristics and mechanism of evolution of the shock wave and the vortex structure in a muzzle jet. The aim was to examine the evolution of the shock wave with initial jet-induced interference. And we established three other simulation models to compare the similarities and differences between the results of the models. The results showed that, in the original complex model, the initial jet restricted the free expansion of the muzzle jet, and this led to many shock–shock collisions that retarded the development of the shock waves. Multiple reflected shock waves were thus formed under a high local pressure that distorted the shock structure, while the structure of the shock wave in the simplified models was clear and simple. The parameters of motion of the body changed by a little when the initial jet-induced interference was ignored. The difference in values of the strongest impact force measured at monitoring points far from the muzzle was small, with an error of about 2%, such that the simplified model without the initial jet could be used in place of the original complex model. The other simplified models yielded significant differences. Our results provide an important theoretical basis for further research on the muzzle jet and its applications in engineering.

The metal-poor atmosphere of a potential sub-Neptune progenitor

AbstractYoung transiting exoplanets offer a unique opportunity to characterize the atmospheres of freshly formed and evolving planets. We present the transmission spectrum of V1298 Tau b, a 23-Myr-old warm Jupiter-sized (0.91 ± 0.05 RJ, where RJ is the radius of Jupiter) planet orbiting a pre-main-sequence star. We detect a mostly clear primordial atmosphere with an exceptionally large atmospheric scale height, and a water vapour absorption at a 5σ level of significance, from which we estimate a planetary mass upper limit (23 Earth masses, 0.12 g cm−3 at a 3σ level). This is one of the lowest-density planets discovered so far. We retrieve a low atmospheric metallicity ($$\log{Z}=-0.{7}_{-0.7}^{+0.8}\,{\mathrm{solar}}$$ log Z = − 0 . 7 − 0.7 + 0.8 solar ), consistent with solar/sub-solar values. Our findings challenge the expected mass–metallicity relation from core-accretion theory. Our observations can instead be explained by in situ formation via pebble accretion together with ongoing evolutionary mechanisms. We do not detect methane, which hints at a hotter-than-expected interior from just the formation entropy of this planet. Our observations suggest that V1298 Tau b is likely to evolve into a sub-Neptune.

Molecular Dynamics Simulation on Solidification Microstructure and Tensile Properties of Cu/SiC Composites

The shape of ceramic particles is one of the factors affecting the properties of metal matrix composites. Exploring the mechanism of ceramic particles affecting the cooling mechanical behavior and microstructure of composites provides a simulation basis for the design of high-performance composites. In this study, molecular dynamics methods are used for investigating the microstructure evolution mechanism in Cu/SiC composites containing SiC particles of different shapes during the rapid solidification process and evaluating the mechanical properties after cooling. The results show that the spherical SiC composites demonstrate the highest degree of local ordering after cooling. The more ordered the formation is of face-centered-cubic and hexagonal-close-packed structures, the better the crystallization is of the final composite and the less the number of stacking faults. Finally, the results of uniaxial tensile in three different directions after solidification showed that the composite containing spherical SiC particles demonstrated the best mechanical properties. The findings of this study provide a reference for understanding the preparation of Cu/SiC composites with different shapes of SiC particles as well as their microstructure and mechanical properties and provide a new idea for the experimental and theoretical research of Cu/SiC metal matrix composites.

Microstructural evolution and spheroidization mechanism of powder metallurgy Ti-6Al-4 V alloys after high-temperature forging

After high-temperature forging and rapidly cooling, the microstructure evolution and spheroidization mechanism of powder metallurgy Ti-6Al-4 V alloy have been clarified. The spheroidization process is divided into two asynchronous stages. The initial stage is primarily characterized by the initial shearing and twisting of GBα grain boundaries, as well as the deformation of lamellar structure. Subsequently, the fragmentation of original GBα grain boundaries and the fracture of intra-granular α lamellae are observed. This process is accompanied by grain twisting and substructure formation, especially the division of lamellae through interface splitting and the segregation of local chemical elements. The differential accumulation of elements around the fractured and twisted grains suggests that element segregation and interfacial energy play a significant role in microstructure evolution. Therefore, the static spheroidization process largely depends on the formation and evolution of dislocation substructures, and the reduction of interfacial energy may be a key factor driving this process. These findings provide a theoretical basis for manufacturing metals with specific anisotropic properties by controlling microstructure evolution.

The polarization-angle flip in GRB prompt emission

In recent years, some polarization measurements of gamma-ray bursts (GRBs) have been reported, and the polarization-angle (PA) rotation in the prompt emission phase has been found in several bursts. The physical mechanism of the PA evolution is still unclear. In this work, we studied the origin of the PA rotation in a toroidal magnetic field. We aim to provide an explanation for the PA rotation in GRBs and find the physical conditions that lead to the rotation by 90 degrees in the toroidal magnetic-field (MF) model. Moreover, we present some observable polarization properties in the MF model that can be tested in the future. We calculated the instantaneous polarization degree (PD) from a top-hat jet with different normalized viewing angles ($q= jet opening angles ($ and jet Lorentz factors (Gamma ) in three wavebands. When the PD changes between positive and negative values, it means that the PA flips by 90 degrees. On these grounds, we can summarize the range of parameters required for these PA flips. Considering these parameter conditions, we can further estimate the observed rate of the GRBs exhibiting such PA rotations. We find that the PA rotation in the toroidal MF is primarily related to three critical factors: the viewing angle, the jet opening angle, and the jet Lorentz factor. Additionally, the PA can experience flips of 90 degrees twice. The conditions for the flips are $q 0.5$ (except for $q 1$) and $y_j =( 4$. However, the two flips in the PA might not be concurrently observable due to the constraint of flux. Taking these conditions into account and assuming a random orientation between the jet axis and the line of sight (LOS), we obtain a theoretical upper limit (without any constraints) for the observed rate of GRBs in the X-ray or gamma -ray band displaying the flips in PA as ch 80<!PCT!>$. We further constrain the observed rate as ch 16<!PCT!>$ according to the maximal post-flip polarized flux level, where the observed rate of single and double flips each account for $ 8<!PCT!>$. It should be noted that the observed rates are different in various wavebands. The observed rate of the second PA flip in the optical bands should be higher than that in the X-ray or gamma -ray band since the flux in the optical band declines much slower than that in the X-ray or gamma -ray band. Moreover, when the LOS is close to the jet edge ($q 1$), it is the easiest case in which to observe the 90-degree PA flip due to the relatively high post-flip polarized flux level. The first and second PA flips in a GRB pulse are most likely to occur at the observed times of obs t_ peak $ and $ t_ peak $, respectively, where $t_ peak $ is the peak time of the pulse. It is also noted that the PA flip would not happen before the peak time.

Mineralization process of the Changjiang uranium orefield in South China: Constraints from pitchblende geochemistry

The Changjiang uranium (U) orefield in northern Guangdong (South China) contains several important granite-related uranium deposits, including the Mianhuakeng deposit. The hydrothermal evolution and mineralization mechanism of the deposit remain unclear, and hence in this study we analyzed the mineral compositional variations of pitchblende from different elevation (−300 to −50 m) in the orebody at Mianhuakeng deposit. The results indicate that with decreasing depth, the hydrothermal mineral assemblage changes from a reducing one (pitchblende, pyrite, and chlorite) to a moderately oxidizing one (pitchblende, coffinite, hematite, and goethite). The contents of U, Sr, ∑REE, U/Th, and LREE/HREE ratios in the pitchblende decrease (whereas the Cu-Pb-Zn-Ni-Co-Sc-Rb contents increase) progressively with decreasing depth. Also, Ce anomaly (Ce/Ce*) changes from positive to negative, and Eu anomaly (Eu/Eu*) becomes more negative with decreasing depth. We interpreted such vertical variations to be caused by the ascent of deep, mid-to-high temperature, highly-oxidizing ore-forming fluid, which was reduced by early-stage reducing minerals in the wallrock, a process that also formed the mineralization at Changjiang. In addition, the pitchblende REE distribution patterns and Eu/Eu* at depth (−300 m) resemble those of the Zhuguang pluton, indicating that the ore-forming materials were originated from the U-bearing granite wallrocks. Therefore, the Ce/Ce* and Eu/Eu* variations of pitchblende can be used to guide deep uranium ore exploration.

Research Status of Friction Interface Evolution Mechanism of Non-magnetic Steel

The non-magnetic drill pipe (composed of male and female thread joints and rod bodies) is the key component of the directional drilling technology equipment for gas extraction in coal mine tunnels. The thread joint and the drill pipe body are connected by continuous drive friction welding. In the study, it was found that the banded structure appeared in the joint of continuous drive friction welding of non-magnetic steel, which reduced the strength of drill pipe. At the same time, it is found that the heat flow mode is one of the key factors affecting the banded structure, and the heat flow mode is based on the evolution of the interface plastic ring. In this paper, through the review of the research status of the interface evolution law of non-magnetic steel continuous drive friction welding, the experimental method for the study of the interface evolution law of non-magnetic steel continuous drive friction welding is found.

KIr4O8 Nanowires with Rich Hydroxyl Promote Oxygen Evolution Reaction in Proton Exchange Membrane Water Electrolyzer.

The sluggish kinetics for anodic oxygen evolution reaction (OER) and insufficient catalytic performance over the corresponding Ir-based catalysts are still enormous challenges in proton exchange membrane water electrolyzer (PEMWE). Herein, we report that KIr4O8 nanowires anode catalyst with more exposed active sites and rich hydroxyl achieves a current density of 1.0 A/cm2 at 1.68V and possesses excellent catalytic stability with 1230h in PEMWE. Combining in situ Raman spectroscopy and differential electrochemical mass spectroscopy results, we propose the modified adsorbate evolution mechanism that rich hydroxyl in the inherent structure of KIr4O8 nanowires directly participates in the catalytic process for favoring the OER. Density functional theory calculation results further suggest that the enhanced proximity between Ir (d) and O (p) band center in KIr4O8 can strengthen the covalence of Ir-O, facilitate the electron transfer between adsorbents and active sites, and decrease the energy barrier of rate-determining step from OH* to O* during the OER. This article is protected by copyright. All rights reserved.

Fracture toughness evolution and failure mechanisms of two-dimensional SiCf/SiC composites at temperatures up to 1500 °C in air

Silicon carbide fiber reinforced silicon carbide composites (SiCf/SiC) are of the few most promising materials for high-temperature structural applications. In this study, two-dimensional (2D) plain-woven SiCf/SiC composites were prepared by chemical vapor infiltration process and subjected to single-sided notched beam three-point bending tests from room temperature (RT) to 1500 °C in air. The temperature-dependent bending load–deflection curves and fracture toughness were obtained. The results show that the SiCf/SiC composites exhibit linear deformation characteristics initially, followed by nonlinear deformation behavior which become more pronounced with increasing temperature. The fracture toughness decreases in the range of RT to 1000 °C due to the evolution of components properties, and increases at 1200 °C due to the interface property degradation and fiber pull-out. Above 1200 °C, it decreases with increasing temperature owing to the serious high-temperature oxidation. Further, the failure mechanisms of the 2D plain-woven SiCf/SiC composites at different temperatures was analyzed through macro and micro analysis. The evolution of components properties affected by high temperature and oxidation as well as fiber pull-out play a leading role in fracture toughness of composites. The findings of this study provide important support for the mechanical behavior and failure mechanisms of SiCf/SiC composites at ultra-high temperatures in air.

Porin Deficiency or Plasmid Copy Number Increase Mediated Carbapenem-Resistant Escherichia coli Resistance Evolution

Aims: This study investigated resistance evolution mechanisms of conjugated plasmids and bacterial hosts under different concentrations of antibiotic pressure. Methods: Ancestral strain ECNX52 was constructed by introducing the bla NDM-5-carrying IncX3 plasmid into E. coli C600, and was subjected to laboratory evolution under different concentrations of meropenem pressure. Minimal inhibitory concentrations and conjugation frequency were determined. Fitness of these strains was assessed. Whole genome sequencing and transcriptional changes were performed. Ancestral host or plasmids were recombined with evolved hosts or plasmids to verify plasmid or host factors in resistance evolution. Role of the repA mutation on plasmid copy number was determined. Results: Two out of the four clones (EM2N1 and EM2N3) exhibited four-fold increase in MIC when exposed to a continuous pressure of 2 μg/mL MEM (1/32 MIC), by down regulating expression of outer membrane protein ompF. Besides, all four clones displayed four-fold increase in MIC and higher conjugation frequency when subjected to a continuous pressure of 4 μg/mL MEM (1/16 MIC), attributing to increasing plasmid copy number generated by repA D140Y (GAT→TAT) mutation. Conclusion: Bacterial hosts and conjugative plasmids can undergo resistance evolution under certain concentrations of antimicrobial pressure by reducing the expression of outer membrane proteins or increasing plasmid copy numbers.

Two Characteristic Patterns of the Summer Indian Ocean Dipole

Abstract This study identifies two distinct patterns of the summer Indian Ocean Dipole (IOD) — the coastal IOD and the offshore IOD — named based on the proximity of their eastern pole to Sumatra. Their spatial characteristics, evolutionary mechanisms, relationships with ENSO, impacts on precipitation, and the factors controlling the simulation performances of climate models are discussed. The coastal IOD shares the same eastern pole as the conventional IOD off Sumatra, but its western pole is located in the central southern tropical Indian Ocean (TIO). The offshore IOD shares the conventional western pole off Somalia, but its eastern pole is located in the central southern TIO. Regarding their evolutions, while they initially develop similarly, their later evolutions differ due to their distinct pole locations: the offshore IOD peaks in summer, while the coastal IOD can be sustained into autumn. The coastal IOD correlates to preceding and late ENSO states, but the offshore IOD does not, making it an independent internal mode of TIO. The two IODs affect climate differently, with only the coastal IOD affecting Australian rainfall. Climate models exhibit varied levels of performance in simulating the two IODs. Specifically, a stronger link between spring TIO rainfall and ENSO, as well as stronger southeasterly monsoonal winds in the southern TIO, can enhance the coastal IOD modeling, while a stronger summer Somali jet benefits the simulation of the offshore IOD. Distinguishing these two IODs has implications for accurate diagnosis and prediction of the summer climate surrounding the TIO.