Impact of oral iron dosing regimens on iron biomarkers during pregnancy: insights from the PANDA trial.

SARS-CoV-2 Ancestral and Omicron variant immunity in Australian children in 2023, a seroprevalence study.

Seroprevalence and predictors of measles antibody positivity among children aged 18-24months in Pakistan: a national survey.

To explore the clinical phenotypes and genetic etiology of a child with MRXS34 syndrome due to a variant of NONO gene. A child patient who presented at Shanxi Provincial Maternity and Child Care Hospital on September 28, 2020 was selected as study subject. Clinical data of the child were retrospectively collected. Peripheral blood samples were collected from the child and his parents. Following extraction of genomic DNA, whole exome sequencing (WES) was carried out. Candidate variant was verified by Sanger sequencing of the family members. Pathogenicity of the variant was assessed based on guidelines from the American College of Medical Genetics and Genomics (ACMG). Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the effect of the NONO gene variant on messenger RNA (mRNA) expression level in the proband, and complementary DNA (cDNA) sequencing was performed to validate the splicing patterns of the NONO gene variant in the proband. Using the keywords "NONO gene" "MRXS34" "developmental delay" "intellectual disability" and "congenital heart disease", a literature search was conducted in databases including the China National Knowledge Infrastructure(CNKI), Wanfang Data and PubMed databases to identify studies on the clinical and genotypic characteristics of children with MRXS34 caused by NONO gene variants. The search period was set from the inception of the databases to April 2025, and a comprehensive analysis of the findings from the identified studies was performed. This study was approved by the Medical Ethics Committee of the hospital (Ethics No.: IRB-KYHZ-2019-006). The proband, a 3-year-old male, exhibited global developmental delay, intellectual disability, facial dysmorphism, macrocephaly, corpus callosum dysgenesis, cavum septum pellucidum, atrial septal defect, tricuspid valve insufficiency with regurgitation, cryptorchidism, inguinal hernia, and anal cutaneous fistula. The results of WES and Sanger sequencing validation showed that the proband carried a heterozygous variant of the NONO gene c.577_581del (p.Val193fs), while both parents were wild-type, indicating that this variant was a de novo variant. According to the ACMG guidelines, this variant was classified as pathogenic (PVS1+PS2_Moderate+PM2_Supporting). RT-qPCR results showed that the relative mRNA expression level of the NONO gene in the proband was significantly lower than that in the control group of normal children, and cDNA sequencing results verified that the frameshift variant due to base deletion led to nonsense-mediated mRNA decay (NMD), causing premature termination of transcription without exon skipping at the splice site. Using the literature search strategy established in this study, a total of 15 studies on the clinical and genotypic characteristics of children with MRXS34 caused by NONO gene variants were identified, involving a total of 32 patients. Together with the proband of this study, a total of 33 patients were included in the comprehensive analysis. The results revealed a total of 23 types of variants involving the NONO gene. The main clinical manifestations of MRXS34 included developmental delay/intellectual disability (23/24, 95.8%), cardiovascular abnormalities (23/30, 76.7%), craniofacial/somatic malformations (21/24, 87.5%), and corpus callosum dysgenesis (16/21, 76.2%). The NONO gene variant probably underlay the pathogenesis of MRXS34 in this proband. The findings of this study has expanded of the variant and clinical spectra associated with the NONO gene.

To analyze the clinical phenotype and genetic etiology of patients with Dyggve-Melchior-Clausen syndrome (DMC syndrome). A child with DMC syndrome diagnosed at Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University in August 2020 was selected as study subject. A retrospective analysis was carried out to collect the proband's clinical data. Peripheral blood samples was collected from the proband and his parents. Following extraction of genomic DNA, whole exome sequencing (WES) was carried out. Candidate variants were validated within the family by Sanger sequencing. Pathogenicity of candidate variants was rated based on guidelines from the American College of Medical Genetics and Genomics (ACMG). This study was approved by the Medical Ethics Committee of the center (Ethics No.: SCMCIRB-K2023024-1). The proband, a 5-year-and-7-month-old girl, presented with short stature (height: -5.5 s) and intellectual disability. Physical examination revealed microcephaly (head circumference: -3.5 s), coarse facial features, long philtrum, pigeon chest, and brachydactyly of both hands. Laboratory findings revealed normal serum insulin-like growth factor-1 (IGF-1) levels (168 ng/mL). Imaging analysis demonstrated dysplasia of corpus callosum and spondyloepiphyseal dysplasia in the proband. WES revealed that she has harbored compound heterozygous variants of the DYM gene, namely c.312-313del (p.His104Glnfs*29) and c.1274A>T (p.Tyr425Phe). Both variants were unreported previously and inherited from her parents who were phenotypically normal. Based on guidelines from the ACMG, the DYM gene variant c.312-313del (p.His104Glnfs*29) was classified as pathogenic (PVS1+PM2_Supporting+PP3+PP4_supporting), while the c.1274A>T (p.Tyr425Phe) variant was classified as likely pathogenic (PM2_Supporting+PP3+PP1+PP4_supporting). By following the pre-set literature search strategy, a total of 20 articles were included, which involved a total of 73 cases of DYM gene variants leading to DMC syndrome. Among these, only one family case was documented in China. Together with proband from this study, a total of 74 DMC syndrome patients due DYM gene variants were included for a comprehensive analysis of clinical phenotypes and genetic characteristics. The age at the time of reporting ranged from 1 to 60 years. The main clinical manifestations included intellectual disability, short stature, and spondyloepiphyseal dysplasia, followed by microcephaly and coarse facial features. By genetic testing, c.1877delA variant was the most common mutation at the nucleotide level. The c.312-313del/c.1274A>T compound heterozygous variants of the DYM gene probably underlay the pathogenesis of DMC syndrome in this proband. Above finding has expanded the mutational and phenotypic spectra of the DMC syndrome.

To investigate epigenetic and transcriptional alterations in children with Wiedemann-Steiner syndrome (WDSTS) due to variants of KMT2A gene using genome-wide DNA methylation array and RNA sequencing (RNA-seq), and identify the key pathways and candidate genes. A retrospective study was carried out for 16 children with WDSTS and 10 healthy controls who visited Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine between November 2016 and December 2024. Peripheral blood samples were collected. Genomic DNA and total RNA were extracted using commercially made kits. Genome-wide DNA methylation profiling was conducted to identify differentially methylated positions (DMPs) and annotated genes, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. RNA-seq was performed to identify differentially expressed genes (DEGs) and conduct GO/KEGG functional annotation. Methylation and expression data were integrated to identify overlapping genes showing significant changes at both levels, followed by GO, KEGG and gene-pathway network analyses. This study was approved by the Ethics Committee of the hospital (Ethics No.: GKLW-A-2024-006-01). A total of 2 652 DMPs corresponding to 1 262 genes were identified, which included 833 hypermethylated genes (66%) and 429 hypomethylated genes (34%). Hypermethylated genes were mainly enriched for functions related to cell junctions, while hypomethylated genes were significantly involved in nervous system development and morphogenesis. RNA-seq identified 2 627 DEGs, including 765 up-regulated genes (29%) and 1 862 down-regulated genes (71%). Up-regulated genes were mainly associated with immune-related processes, and down-regulated genes were mainly related to substance transport. Integrative analysis identified 93 overlapping genes with significant changes in both methylation and expression. And these genes were enriched in extracellular matrix-related processes, calcium ion binding, neurodevelopment, and cell adhesion. Key candidate genes, including LAMB1, LAMB2 and NID1, were further prioritized. Integrated analysis of DNA methylation and transcriptome data reveals WDSTS-related epigenetic-transcriptional alterations and provides clues for exploring disease mechanisms and optimizing diagnostic strategies.

To explore the clinical phenotype and genetic etiology of a fetus with autosomal dominant intellectual disability type 72 (MRD72) resulting from a variant of the SRRM2 gene. A Chinese pedigree with MRD72 (fetus) who had visited the Affiliated Women and Children's Hospital of Ningbo University in November 2024 was selected as study subject. Clinical data of the pedigree were collected. Amniotic fluid and peripheral blood samples were collected from the fetus and its parents for genomic DNA extraction. Whole-exome sequencing (WES) was carried out, and candidate variants were verified by Sanger sequencing of the family members and rated based on guidelines from the American College of Medical Genetics and Genomics (ACMG). Relevant literature on MRD72 were searched in domestic and international databases for a review. This study was approved by the hospital (Ethics No.: EC2023-094). The proband was a fetus of 25 weeks of gestation. Fetal echocardiography revealed a relatively small left atrium and left ventricle, along with a diminished aortic-to-pulmonary artery ratio. WES revealed that the fetus has harbored a heterozygous nonsense variant of the SRRM2 gene. Sanger sequencing confirmed both parents carried the wild-type alleles. Based on guidelines from the American College of Medical Genetics and Genomics (ACMG), the variant was rated as likely pathogenic (PVS1+PM2_Supporting) and has not been recorded in public databases. Bioinformatic analysis predicted amino acid 512 to be highly conserved across various species. According to the pre-set literature search strategy, 4 publications were retrieved, which involved 30 MRD72 patients from 27 pedigrees, In addition to this study, a total of 31 cases were included. Analysis of clinical features and genetic etiology showed that patients with MRD72 presented mainly with clinical manifestations such as mental and motor development delay, special facial features, speech/intellectual development delay, and obesity. The genetic etiology was all variants at relevant loci of the SRRM2 gene. The SRRM2 variant identified in this study is implicated as the genetic cause of MRD72 in the proband. Above results have expanded the mutational and phenotypic spectra of the SRRM2 gene.

Blood coagulation analysis is essential for evaluating bleeding and clotting risks, particularly in extracorporeal life support (ECLS) patients receiving preventative anticoagulant therapy, like heparin or bivalirudin. Therapy management requires continuous monitoring; however, existing methods exhibit high variability and require relatively large blood volumes, limiting their use in neonatal and pediatric care. This study assesses the ability of integrated quasistatic acoustic tweezing thromboelastometry (i-QATT), a novel noncontact technique utilizing small blood samples (6 µl), to monitor anticoagulant therapy. Integrated quasistatic acoustic tweezing thromboelastometry analysis was conducted on platelet-poor plasma samples collected from pediatric ECLS patients treated with unfractionated heparin (UFH) or bivalirudin, while heparinase was applied to neutralize the anticoagulation effect of UFH. Integrated quasistatic acoustic tweezing thromboelastometry accurately detected UFH and bivalirudin effects and UFH reversal in commercial and patient plasma samples, where clot initiation time, clotting time, and time to firm clot formation were identified as key i-QATT parameters in anticoagulant monitoring. The technique demonstrated sensitivity to anticoagulant dosage levels, effectively distinguished between different anticoagulants, and exhibited strong correlations with gold-standard plasma coagulation tests and rotational thromboelastometry. Additionally, i-QATT showed potential in assessing dynamic changes in thrombosis during the treatment period. The findings of this study highlight i-QATT's ability to monitor anticoagulant therapy in pediatric patients.

Methodologic feasibility of comprehensive biomarker collection in a pilot trial of a novel psychotherapy for trauma-related nightmares.

Background: Zearalenone (ZEN) is a mycotoxin that the fungus produces Fusarium spp., which reaches humans through food contaminated with mycotoxins. The purpose of this study is to look into the presence of zearalenone in the serum among females suffering from polycystic ovary syndrome, how it influences the levels of FSH, or follicle-stimulating hormone and estrogen, and how it relates to CYP19 gene polymorphisms. Methodology: This was accomplished by obtaining blood samples from both healthy women and women with polycystic ovarian syndrome and measuring zearalenone, estrogen, and FSH using ELISA. PCR was also employed to examine the CYP19 gene's SNPs utilizing the HSP92ll enzyme. Results: The findings show that the amount of zearalenone in polycystic ovarian syndrome women's serum (4.03 ± 1.65 ng/ml) was greater than the healthy women (0.5 ± 0.1 ng/ml), Additionally, It was noted that samples of women with PCOS had higher levels of estrogen (60.86 ± 18.27 pg/ml) than samples of healthy women (48.82 ± 17.90 pg/ml). In contrast, samples of blood from polycystic ovarian syndrome patients had lower levels of FSH (5.22±1.56 ml.U/ml) than samples from healthy women (7.55±1.73 ml.U/ml). The PCR results using the HSP92ll enzyme indicate the presence of three the genotype AA (homozygosity) AG (heterozygosity) GG (homozygous) The genotypes in women with PCOS indicate that they are GG, AG, AA (38, 43, 19)% compared to healthy women (20, 35, 45)%, respectively, Conclusions: This study indicates the presence of the toxin zearalenone in PCOS-affected women's blood serum. Toxins in the serum contribute to PCOS.

Wild animals often play a role in the transmission of zoonotic pathogens. Among the wild mammals of South America, ring-tailed coatis (Nasua nasua) can present a high frequency of anti-Leptospira antibodies and carry Leptospira spp. This study aimed to evaluate the frequency of serum anti-Leptospira antibodies and perform the molecular identification of Leptospira spp. in ring-tailed coatis living in the Tietê River Ecological Park, in southeastern Brazil. Blood and urine samples were obtained from ring-tailed coatis. The serum was tested using the Microscopic Agglutination Test (MAT). Urine was cultured in semi-solid Ellinghausen-McCullough-Johnson-Harris medium. The inoculum was cultivated at 28°C and monitored over six weeks. Total DNA from the blood and urine samples was extracted, and the 16S rRNA gene and lipL32 were searched by PCR. Of 192 animals, 36 (18.75%) had positive serum samples in the MAT. The most frequent serogroups in MAT were Grippotyphosa (11.46%; 22/192), Autumnalis (7.81%; 15/192) and Cynopteri (2.60%; 5/192). A total of 116 urine samples were obtained. None of the Leptospira spp. were isolated, twelve samples were positive for the 16S rRNA gene (10.34%), and four were positive for the lipL32 gene (3.4%). These results indicate a high frequency of serum anti-Leptospira spp. antibodies in coatis from the Tietê River Ecological Park and highlight the possibility of their potential role as carriers of pathogenic Leptospira spp. in urine.

Current research provides limited evidence for connections between metal exposure, as an emerging environmental risk factor, and obstructive sleep apnea (OSA) risk. This study aimed to examine the associations between blood and urine metal exposure and OSA risk. This cross-sectional study recruited participants and extracted data from the 2017-2020 round survey of the National Health and Nutrition Examination Survey. OSA was ascertained using the STOP questionnaire. Eight metals in blood samples were detected in 3,857 participants, and seven metals in urine samples from 1,902 participants. The combined effects of blood or urinary metal mixtures on OSA were assessed using Weighted Quantile Sum (WQS) regression and Quantile-based g-computation (Qgcomp) models. Odds ratio (OR) with 95% confidence interval (CI) was estimated using a weighted logistic regression model. Restricted cubic spline (RCS) models were applied to display the nonlinear associations. Both the WQS model (OR: 1.362, 95% CI: 1.123-1.652) and the Qgcomp models (OR: 1.259, 95% CI: 1.070-1.483) revealed that urinary metal mixtures were positively associated with OSA, with mercury and nickel contributing the highest weights. For each metal, RCS models were applied but significant nonlinear associations were only observed between blood mercury and urinary chromium and OSA risk. In single-metal models, most individual metals did not show significant associations after adjustment. This study identified nonlinear associations between blood mercury and urinary chromium concentrations and OSA risk, and exposure to mixtures of urinary metals was associated with an increased OSA risk.

Adaptive traits, along with productive and reproductive performance, are essential for assessing the suitability and long-term sustainability of indigenous cattle in specific production environments. This study evaluated the adaptive, productive and reproductive performance of Boran dairy cattle in the lowland agro-ecology of the Kaffa Zone, south-western Ethiopia. The study was conducted at a commercial farm in Gojeb, and sixty (60) multiparous Boran dairy cows were selected based on health status, parity, and lactation stage, following an adaptation period before data collection. This study covered the early, mid and late lactation stages over a 25-week monitoring period. Daily milk yield was recorded twice daily, and reproductive parameters, including age at first calving (AFC), calving interval (CI), number of services per conception (NSPC), days open (DO) and gestation length (GL), were obtained from farm records. Adaptive performance was assessed using rectal temperature (RT) and respiration rate (RR) in relation to the meteorological variables. Blood samples were collected from 30 cows (10 per lactation stage) for haematological (HC, RBC, WBC and PCV) and biochemical (TP, GLU, URE, TGL, TC, AST and ALT) analyses. Data were analysed using SPSS version 20, and descriptive statistics were summarized as mean ± SD. The current study revealed that the mean daily milk yield was 2.43 ± 0.62, 2.12 ± 0.58 and 1.72 ± 0.45 L/day for early, mid and late lactation, respectively. Parity 1 and 2 cows produced 1.71 ± 0.46 L and 2.47 ± 0.52 L, respectively, with an overall mean of 2.09 ± 0.62 L/day. Parity and lactation stage significantly (p < 0.001) affected production performance. The reproduction performance of Boran dairy cows was as follows: AFC (51.63 ± 1.82 months), CI (19.07 ± 1.93 months), GL (276.50 ± 2.15 days), DO (338.05 ± 21.71 days) and NSPC (1.60 ± 0.22). Lactation stage and parity significantly influenced several biochemical (TP, GLU, TGL, TC, ALT and AST) and haematological (HC, WBC and PCV) parameters, with total protein and urea being the main contributors to blood profile variation. Blood values remained within the normal range, indicating good physiological adaptation to the local conditions. The productive and reproductive performances of Boran cows were within the expected ranges for indigenous breeds, suggesting that crossbreeding could enhance productivity. Further studies should consider age, season and comparisons with other locally adapted breeds.

Biological subphenotypes in severe acute hypoxaemic respiratory failure and acute respiratory distress syndrome using rapid prospective classification (SPARC) in the USA: a multicentre, observational, study.

Diacerein protects against thioacetamide-induced acute liver injury via modulating HMGB1/TLR4/MyD88/NF-κB signaling pathway.

Effective postoperative pain management is challenging because of adverse effects associated with traditional analgesic methods. Multimodal analgesia, targeting multiple pain pathways, offers improved pain relief while reducing side effects. The authors evaluated the analgesic and anti-inflammatory effects of the ReproGel drug delivery system (DDS) combined with ropivacaine in a rat model of postoperative pain. Sixty-four Sprague-Dawley rats were randomly assigned to four groups: sham treatment, ReproGel DDS, ropivacaine 0.375%, and ReproGel DDS + ropivacaine 0.375%. Mechanical withdrawal threshold (MWT) tests and rotarod assessments were conducted at baseline; at 30, 60, 90, 120, 180, and 240 minutes; at 24 and 48 hours; and at 1 week postoperatively. Blood samples were collected at 24, 48 hours, and 1 week to measure levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 before euthanasia. The ReproGel DDS + ropivacaine group demonstrated significantly higher MWT values, demonstrating enhanced analgesia from 30 to 240 minutes postoperatively. Combination treatment with ReproGel demonstrated superior effectiveness at 240 minutes, outperforming ropivacaine alone, indicating a sustained analgesic effect. IL-1β and IL-6 levels were significantly reduced in groups receiving ropivacaine, particularly in the ReproGel DDS + ropivacaine group, at 24 and 48 hours postoperatively. No motor impairment was observed, confirming the safety of ReproGel DDS + ropivacaine in preserving motor function. The combination of ReproGel DDS and ropivacaine 0.375% in a postoperative rat model offers prolonged analgesic and anti-inflammatory effects without motor impairment, positioning it as a safe and effective option for postoperative pain management.

Current data demonstrate that early blood product resuscitation and minimization of crystalloid improve outcomes. However, there is little data on crystalloid use after hemorrhage control. We hypothesized that administration of crystalloid during early post resuscitation care may contribute to ongoing endothelial dysfunction. As part of a prospective observational study of patients in hemorrhagic shock, crystalloid data were collected upon arrival, in the operating room and during the first 24, 48, and 72 hours in the intensive care unit (ICU). Blood samples were collected on arrival and in a subset of patients at 24 hours. Indicators of inflammation, endothelial dysfunction, and coagulation were evaluated by Luminex. Demographics, physiological data, outcomes, blood product, and resuscitation data were abstracted. A total of 69 patients met the inclusion criteria. The mean crystalloid volume in the first 24 hours was 4.7 L (3.5-7 L): 0.5 L (0-1.5 L) during trauma resuscitation, 2.5 L(1.7-3.5 L) in the operating room and 1.5 L in the ICU. Cumulative total was 6.9 L (4.5-9.2 L) at 48 hours and 7.7 L (6.2-9.8 L) at 72 hours. At admission, patients had increases in endothelial, coagulation, and inflammatory markers compared with minimally injured controls. There was a correlation between crystalloid and syndecan-1 and INR at 24 hours, suggesting ongoing endothelial dysfunction. At 48 and 72 hours crystalloid totals correlated with sequential organ failure scores, ICU length of stay, and ventilator days. We continue to rely on crystalloids after hemorrhage control. This practice is associated with ongoing endothelial and coagulation dysfunctions and suggests the need for alternative resuscitation strategies in the post hemorrhagic phase of care.

Cardiac damage predicts poor outcomes in polytrauma (PT). In older patients, cardiovascular risk factors may predispose to post-traumatic cardiac dysfunction. This study examined whether cardiovascular risk correlates with cardiac damage and influences clinical outcomes in PT. This study at a German Level 1 Trauma Centre enrolled 59 PT patients upon emergency room admission. Blood samples were taken at the emergency room, 24, 48, 72, 96 hours, and 10 days to assess cardiac damage via troponin T and N-terminal pro-B-type natriuretic peptide. Transthoracic echocardiography (TTE) was performed at 24/48 hours. Cardiovascular risk was evaluated using the Systematic Coronary Risk Evaluation (SCORE)2 algorithm. Subgroup analysis compared cardiac damage in patients with high (SCORE2 >7.5%) versus low risk, and assessed the additional impact of chest trauma. Arrhythmias were observed in 39% of patients, whereas acute repolarization disorder occurred in nearly 19%. TTE revealed wall motion abnormalities in 12%, diastolic dysfunction in 10%, and right ventricular dysfunction in 5%. SCORE2 and lipoprotein(a) significantly correlated with serum levels of troponin T and N-terminal pro-B-type natriuretic peptide. SCORE2 values were associated with nonsurvival, wall motion disorders, diastolic dysfunction, and relaxation disorders ( P < 0.05). A higher incidence of arrhythmias, diastolic dysfunction, and relaxation disorders was observed in the subgroup of high-risk patients with chest trauma. Patients in the high-risk group without chest trauma showed higher nonsurvival rates (50%), which may be strongly influenced by a history of myocardial infarction. Cardiovascular risk was significantly associated with cardiac damage markers, TTE abnormalities, and increased mortality. A history of myocardial infarction was associated with higher mortality in PT patients with an elevated SCORE2 risk.

Background: Bovine anaplasmosis is a communicable disease transmitted via ticks, which carries significant veterinary and economic implications. Data from western Iraq remains scarce despite high cattle exposure to vector-borne infections. Objective: To determine the incidence of Anaplasma marginale in cattle in Anbar Province, compare the diagnostic performance of microscopy, ELISA, and PCR, and assess associated haematological changes. Methodology: Between January and December 2024, three hundred blood samples were drawn from cattle (1–10 years, both sexes) across 20 farms in Haditha, Heet, Al-Qaim, and Rutba districts; Giemsa-stained smears, a commercial ELISA, and PCR targeting the msp5 gene were used for diagnosis. Haematological parameters (RBC, Hb, PCV, WBC) were measured using an automated analyser. Prevalence was determined by sex, age, and season, while haematological data were compared by t-tests (α = 0.05). Results: Prevalence was 28.0% by microscopy, 36.3% by ELISA, and 41.3% by PCR, with PCR being most sensitive. Infection rate was higher among females (46.9%) compared to males (33.6%), and in older cattle than in younger ones; Peak rates occurred in the summer (62.6%), with the lowest rates in winter (17.3%). Infected cattle had reduced Hb, PCV, RBC, and elevated WBC counts. Conclusion: This study provides the first detailed epidemiological report on bovine anaplasmosis in western Iraq. The results confirm that PCR is the most sensitive tool, underlining host and seasonal influences on infection and revealing distinct haematological alterations. These findings provide a surveillance baseline and support control strategies against A. marginale in endemic regions.

Chronic hepatitis D is the most severe form of viral hepatitis. Despite recommendations for systematic screening of HBsAg-positive individuals, hepatitis D infection remains underdiagnosed. Standard HDV virological markers are classically assessed in serum or plasma specimens obtained through venous whole blood sampling. This approach is costly and challenging in resource-limited settings. Dried blood spot (DBS) sampling offers a practical alternative for large-scale screening, diagnosis, and monitoring of viral hepatitis. The goal of the study is to evaluate the performance of commercially available HDV diagnostic assays using DBS. Plasma and DBS-collected whole blood specimens from individuals chronically infected with HBV or coinfected with HBV and HDV were analysed for HDV antibody detection, HDV RNA quantification, HBsAg levels, and HDV genotyping. HDV antibodies were reliably detected in DBS after threshold adjustment, with the LIAISON XL Murex Anti-HDV assay showing superior sensitivity (99.3%) compared to HDV Ab DIA.PRO (90.3%), while both assays exhibited excellent specificity. HDV RNA was quantifiable in 86.8% of DBS specimens from patients with active infection, although levels were about 1.5 log10 lower than plasma, showing a strong correlation (r = 0.786; p < 0.0001). HBsAg was detectable and quantifiable in all DBS samples, correlating closely with plasma values (r = 0.98). DBS-based HDV genotyping was successful in 84.8% of samples and concordant with plasma results. DBS collection of whole blood from DBS appears to be a promising and practical alternative to conventional venous blood sampling for HDV screening, diagnosis, and monitoring.