Metabolomic profiling of Piper sarmentosum Roxb. Extracts reveals potent xanthine oxidase inhibition and anti-inflammatory effects.

Dietary thiamine supplementation enhances the growth performance, digestive enzyme activity, intestine development, immunity and anti-inflammatory gene expression of juvenile olive flounder (Paralichthys olivaceus).

Introduction: Antioxidants play a crucial role in maintaining oral health by counteracting oxidative stress and Reactive Oxygen Species (ROS) to combat dental diseases. The present Investigated the antioxidant properties of an herbal formulation derived from African basil and black tulsi, both individually and in nanoparticle-based formulations. The present study addressed the need for advanced, sustainable antioxidant therapies by exploring the synergistic potential of nanoparticles synthesised via green methods from Ocimum tenuiflorum (African tulsi) and Ocimum gratissimum (black tulsi) extracts. By combining the bioactive properties of these herbs with the unique capabilities of nanoparticles, the research goals to enhance antioxidant efficacy and offer innovative solutions for oxidative stressrelated dental and biomedical applications. Aim: To evaluate the efficacy of Ocimum gratissimum and Ocimum tenuiflorum herbal formulation and its mediated nanoparticles and nanocomposite in neutralising oxidative stress and their potential as advanced antioxidant products. Materials and Methods: The present in-vitro study, conducted between January and June 2023 at Nanobiomedicine lab, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, India, evaluated the antioxidant properties of a herbal formulation derived from Ocimum tenuiflorum (black tulsi) and Ocimum gratissimum (African basil) both individually and in nanoparticlebased formulations. The study included herbal extracts with significant phytochemical content and the methodology involved green synthesis of silver (AgNPs) and Zinc Oxide Nanoparticles (ZnONPs) using the herbal formulation, followed by their characterisation and evaluation via 2,2-diphenyl-1- picrylhydrazyl (DPPH), Hydrogen Peroxide (H2 O2 ), and Ferric Reducing Antioxidant Power (FRAP) assays. Results: The herbal formulation exhibited antioxidant activity in all assay when compared to the standard antioxidant (ascorbic acid), which achieved a maximum inhibition of 92.4 at 50 µg/ mL. Incorporation into nanoparticle structures led to further enhancement of antioxidant activity, with the nanocomposite demonstrating the highest antioxidant activity among all samples tested, achieving 90.91 inhibition in the DPPH assay, 86.5 inhibition in the H2 O2 assay, and a FRAP value of 86.81 at 50 µg/mL, compared to ascorbic acid. Due to the use of a single sample per concentration, p-value cannot be computed and the results are demonstrated using bar graphs. Conclusion: The findings of this study provide compelling evidence of the potent antioxidant properties of the herbal formulation derived from African basil and black tulsi, particularly when incorporated into nanoparticle-based formulations. These results suggest that herbal-nanoparticle combinations could serve as effective alternatives or supplements to traditional antioxidants, offering a natural and sustainable approach to combating oxidative stress.

Introduction: Inflammation and oxidative stress play key roles in the pathogenesis of various musculoskeletal and soft tissue disorders. Conventional pharmacologic therapies, such as Nonsteroidal Anti-Inflammatory Drugs (NSAIDs), are associated with several adverse effects, prompting interest in safer, biologically active alternatives. Trypsin and bromelain are proteolytic enzymes known for their anti-inflammatory activity, while rutoside exhibits potent antioxidant and vascular protective properties. Glucosamine, commonly used for joint health, contributes to cartilage repair and has shown mild anti-inflammatory effects. The combination of these agents in a topical gel formulation may offer synergistic benefits in managing inflammation and oxidative stress locally. Aim: The present study examined the anti-inflammatory and antioxidant properties of trypsin, bromelain, rutoside, and glucosamine combination. Materials and Methods: The present in-vitro study was conducted at Gold lab, Department of Pharmacology, Saveetha Dental College and Hospital, Chennai, Tamil Nadu, India, from January to August 2024. The chemicals involved in the process were sourced from SISCO Research Laboratories private limited. The active pharmacological ingredients like trypsin, bromelain, rutoside and glucosamine were mixed and tested. The anti-inflammatory and antioxidant properties of both the formulations were investigated. Albumin denaturation assay for anti-inflammatory activity and 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) assay for antioxidant activity were performed. Student t-test was performed to determine the significance, where p-value less than 0.05 were considered statistically significant. Results: The combination gel exhibited a dose-dependent increase in both anti-inflammatory and antioxidant activities. Anti-inflammatory activity, assessed via the albumin denaturation assay, increased from 51.74% at 20 µg/mL to 85.02% at 100 µg/ mL, closely approaching the inhibition shown by the standard drug diclofenac sodium (94.53%). Similarly, in the DPPH assay, antioxidant activity rose from 41.26% at 20 µg/mL to 95.98% at 100 µg/mL, surpassing the activity of ascorbic acid (86.86%) at the highest concentration. Statistical analysis confirmed that these increases were highly significant (p<0.05). Conclusion: The in-vitro findings demonstrated that the trypsin, bromelain, rutoside, and glucosamine combination gel possessed strong, concentration-dependent anti-inflammatory and antioxidant properties. These results suggest its potential as a topical therapeutic alternative to conventional NSAIDs for localised treatment of musculoskeletal and inflammatory conditions. Further in-vivo and clinical studies are recommended to validate its safety, efficacy, and therapeutic applicability

Context: Witheringia solanacea has traditionally been used in Latin American medicine for its anti-inflammatory and antimicrobial properties, as well as for general pain management. However, few pharmacological studies have been conducted to validate these traditional uses. Aims: To determine the chemical composition and evaluate selected pharmacological activities of W. solanacea extracts using various experimental models. Methods: Hydroalcoholic extracts from fruit and aerial parts of W. solanacea were analyzed by high-performance thin-layer chromatography to detect secondary metabolites. Qualitative phytochemical screening and quantification of total phenolic and flavonoid contents were performed. The antioxidant and antibacterial activities of the extracts were evaluated in vitro. Additionally, acute oral toxicity, analgesic, and anti-inflammatory activities of the aerial part extract were assessed in rats. Results: Phytochemical analysis confirmed the presence of phenolics, flavonoids, coumarins, terpenoids, triterpenes, steroids, and alkaloids. Fruit extracts exhibited higher antioxidant activity than aerial-part extracts. The extracts showed only limited antibacterial activity, with effects observed only against E. faecalis (MIC ≈ 5 mg/mL). The aerial-part extract was classified as non-toxic (LD₅₀ > 2000 mg/kg). In vivo, this extract produced significant analgesic effects in the tail-flick model and significantly reduced carrageenan-induced paw edema and leukocyte infiltration, with effects comparable to those of indomethacin. Conclusions: W. solanacea aerial part extract exhibits analgesic and anti-inflammatory properties that support its traditional use for pain and inflammation, although only limited antibacterial activity was observed.

Introduction: Inflammation and oxidative stress play key roles in the pathogenesis of various musculoskeletal and soft tissue disorders. Conventional pharmacologic therapies, such as Nonsteroidal Anti-Inflammatory Drugs (NSAIDs), are associated with several adverse effects, prompting interest in safer, biologically active alternatives. Trypsin and bromelain are proteolytic enzymes known for their anti-inflammatory activity, while rutoside exhibits potent antioxidant and vascular protective properties. Glucosamine, commonly used for joint health, contributes to cartilage repair and has shown mild anti-inflammatory effects. The combination of these agents in a topical gel formulation may offer synergistic benefits in managing inflammation and oxidative stress locally. Aim: The present study examined the anti-inflammatory and antioxidant properties of trypsin, bromelain, rutoside, and glucosamine combination. Materials and Methods: The present in-vitro study was conducted at Gold lab, Department of Pharmacology, Saveetha Dental College and Hospital, Chennai, Tamil Nadu, India, from January to August 2024. The chemicals involved in the process were sourced from SISCO Research Laboratories private limited. The active pharmacological ingredients like trypsin, bromelain, rutoside and glucosamine were mixed and tested. The anti-inflammatory and antioxidant properties of both the formulations were investigated. Albumin denaturation assay for anti-inflammatory activity and 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) assay for antioxidant activity were performed. Student t-test was performed to determine the significance, where p-value less than 0.05 were considered statistically significant. Results: The combination gel exhibited a dose-dependent increase in both anti-inflammatory and antioxidant activities. Anti-inflammatory activity, assessed via the albumin denaturation assay, increased from 51.74% at 20 µg/mL to 85.02% at 100 µg/ mL, closely approaching the inhibition shown by the standard drug diclofenac sodium (94.53%). Similarly, in the DPPH assay, antioxidant activity rose from 41.26% at 20 µg/mL to 95.98% at 100 µg/mL, surpassing the activity of ascorbic acid (86.86%) at the highest concentration. Statistical analysis confirmed that these increases were highly significant (p<0.05). Conclusion: The in-vitro findings demonstrated that the trypsin, bromelain, rutoside, and glucosamine combination gel possessed strong, concentration-dependent anti-inflammatory and antioxidant properties. These results suggest its potential as a topical therapeutic alternative to conventional NSAIDs for localised treatment of musculoskeletal and inflammatory conditions. Further in-vivo and clinical studies are recommended to validate its safety, efficacy, and therapeutic applicability

Quercetin from Penthorum chinense Pursh protects against LPS-induced liver inflammation in chickens via suppressing pyroptosis-macrophage polarization crosstalk.

Polymer encapsulation of Cynara Scolymus L. extract using supercritical fluid expansion into an aqueous solution (ESSAS): Optimization of conditions, identification of compounds using LC-HRMS, and investigation of antioxidant properties.

Enhancing wound dressing efficiency: Cellulose nanofiber sponges loaded with Ganoderma lucidum mycelium fractions.

Emodin-derived red-emissive carbon dots: Light-driven ROS generation and antioxidant activities in non-illuminative regimes.

Impact of dendrobium nobile supplementation on antioxidant activity and egg-laying performance in late-laying hens

The effect of Spirulina platensis on water quality factors, biofloc composition, growth efficiency, fatty acid and amino acid profile of fillets, carcass composition, antioxidant activities, bacterial population, and gene expersion in common carp (Cyprinus carpio) reared in a system without water exchange

Protective role of Oxyresveratrol against NaIO3-induced oxidative stress in RPE cells via targeting NRF2-mediated ferroptosis in vitro and in vivo.

A sustainable triple-action inhibitor: Corrosion protection, antioxidant activity, and antibacterial performance of Solanum chrysotrichum fruit extract.

A heterogeneous hydrogel patch with mechanical activity and bioactivity for chronic diabetic wound healing.

An antioxidant metal-organic framework with functional coatings for oral anti-TNF-α antibody delivery in inflammatory bowel disease treatment.

Resveratrol mediated photodynamic inactivation using UVA light on foodborne pathogens.

Bacterial cellulose-based multifunctional films modified with natural Sapindus saponin: a sustainable food packaging material.

Oligophenylvinylene-derived fluorescent sensor: Cyanide recognition and its anti-inflammatory, antidiabetic, and antioxidant activities

Neuropharmacological potentials (antipsychotic-, anxiolytic-, and antidepressant-like activities) of methanol leaf extract of Andrographis paniculata Nees in vivo: Possible mechanisms, antioxidant activity, and in silico supportive evidence.