Animal Body Research Articles

The effect of exposure to RF-EMF from the laboratory simulator of 5G NR base station on physiological parameters and cognitive abilities of male wistar rats of different ages

In this article, the impact of radiofrequency electromagnetic field (RF-EMF) exposure from a simulated base station for the 5G New Radio (5G NR) telecommunication on rats was studied. The base station affects all age groups of the population, thus, for the first time, the experiment was conducted on male Wistar rats of three different ages (juvenile, adult, and presenile). The base station exposure parameters were chosen according to ICNIRP recommendations for limiting the exposure to radiofrequency electromagnetic field: frequency 2.4 GHz with an average specific absorption rate of 0.0076 W/kg and 0.0059 W/kg over the whole body of experimental animals. Throughout the experiment, body weight was examined weekly, and the dynamics of body weight gain was monitored. Rectal and skin surface temperature on the right hind limb was monitored weekly. Testing in the Morris water maze was performed during the last, Week 5, of RF-EMF exposure. After euthanasia, organ weights were determined in experimental and control animals. None of the investigated parameters did show any statistically significant differences between exposed and control animals of the same age. The data obtained can be used to assess the possible consequences of chronic exposure to RF-EMF from 5G NR base stations.

Between social cognition and material engagement: the cooperative body hypothesis

AbstractIn recent years, social cognition approaches to human evolution and Material Engagement Theory have offered new theoretical resources to advance our understanding of the prehistoric hominin mind. To date, however, these two approaches have developed largely in isolation from one another. I argue that there is a gap between social- and material-centred approaches, and that this is precisely the sociomateriality of the appearance of ancestral hominin bodies, which evolved under selective pressure to develop increasingly complex, cooperative sociality. To get this sociomaterial body in focus, I develop an esthesiological framework, appropriated from Merleau-Ponty (2003), for interpreting the expressive body in an evolutionary and comparative context. The guiding hypothesis of esthesiology is that before being rationality (social or material), “humanity is another corporeity” (Merleau-Ponty, 2003, p. 208). Esthesiology studies the appearance of the body and its sense organs as an intertwining locus of a sensing power (the ability to see, to touch, etc.) and a sensible character (the visible, touchable body). It is this dual-aspect character of the body that facilitates the most basic affective and sensorimotor modes of sociality. Examining these features from a comparative perspective, we find that the human body is distinctively suited to prosocial communication and cooperation: a more cooperative eye, an exposed and communicative skin. I thus propose a cooperative body hypothesis, by analogy with the cooperative eye hypothesis (Tomasello et al., 2007). Esthesiology provides a framework for integrating and interpreting a wide range of otherwise disconnected facts concerning human and nonhuman animal bodies, forms of life, cognition, and evolution, thereby bridging the gap between social cognition and material engagement perspectives. In doing so, however, it not only solves problems and proposes new directions of investigation, but also demands theoretical revisions from each.

Systematic Assessment of Bottomland Resources Influenced by Waste Disposal

In Ile-Oluji, Nigeria, issues with waste disposal brought on by population growth have resulted in contamination of the bottomland  resources that sustain humankind. Random soil samples were taken from a solid dumpsite and its adjacent closed bottomland at depths  of 0–15, 15–30, and 30–75 cm and analyzed in the laboratory. Soil texture, soil moisture content (MC), porosity, bulk density, pH, electrical  conductivity (EC), organic carbon (OC), potassium (K), calcium (Ca), magnesium (Mg), and sodium (Na) were measured. Also examined  was the concentration of heavy metals such as lead (Pb), cadmium (Cd), arsenic (As), nickel (Ni), chromium (Cr), and manganese (Mn) in  the samples. Soil moisture content (MC), porosity, bulk density, pH, and electrical conductivity (EC) ranged from 8.52 to 16.08%, 30.14 to  51.19%, 1.16 to 1.46 g/cm3, 6.98 to 7.58, and 299.82 to 432.61 μS/cm, respectively. The concentrations of nutrients and heavy metals (OC,  K, Ca, Mg, and Na) and (Pb, Cd, As, Ni, Cr, and Mn) in the soil samples collected from the solid waste dumpsite and bottomland ranged respectively from 2.13–3.10%,, 3.98–6.57, 5.08–8.11, 5.41–7.11, and 0.98–2.61 Cmol/kg, and 6.83– 29.04, 0.00–0.35, 0.41–3.92, 0.89–3.46,  0.00–0.63, and 13.74–74.38 mg/kg. The highest concentrations of nutrients and heavy metals were found in the soil sample collected from  the solid waste dumpsite between 0 and 15 cm of soil depth, and it was closely followed by the sample obtained from the bottomland at the same soil depth compared to other locations. The concentration of heavy metals did not exceed the limits established  by the World Health Organization (WHO) and the Food and Agricultural Organization (FAO), but persistent dumping of waste in the area  may eventually increase the concentration of heavy metals beyond the acceptable limit. There is a need for the locals to be aware that the  growing of crops on dumpsites and closed adjacent bottomland due to its fertility status could result in the uptake of heavy metals  and eventually end up in human or animal bodies. A measure to avoid dumping waste close to the bottomland should be put in place by  ensuring the immediate construction of a standard environmental sanitary dumpsite in combination with the waste recycling strategy in  the study area. 

The mechanism of interaction of the animal›s body with the microbiota of the gastrointestinal tract (review)

The intestines of animals are a complex ecosystem consisting of a microbiome, host cells, and nutrients. There are about 100 trillion bacteria in the intestinal tract that form the intestinal microbiota. They are taxonomically classified by genus, family, order, and type. The intestinal microflora mainly consists of six types: Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Fusobacteria and Verrucomicrobia. Bacterioidetes and firmicutes occupy a dominant position in the intestinal tract of animals and play a key role in the nutrient absorption system and help strengthen the intestinal barrier. The composition of the microbial community ecosystem is dynamic, and its composition depends on many factors including genes, medications, and feeding.The purpose of this review is to analyze the microbiome of the gastrointestinal tract of animals and factors affecting their biodiversity. The composition of the microbial community ecosystem is dynamic and depends on many factors, including genes, medications, and feeding. Changes in the feeding diet can cause time shifts (within 24 hours) in a large number of microorganisms. It follows that feeding is also a vital regulator of the intestinal microbiota. The prospects for applying the results of the study of the microbiota of the body of farm animals are obvious, since they can become the basis for creating technologies that allow correcting undesirable changes in the microbiome of animals that arise as negative consequences of the intensification of highly productive agricultural production.

Regularities of Growth and Development of Buffaloes

Based on the research, an analysis of the biological characteristics of the growth and development of buffaloes in certain periods and stages was carried out. The growth rate of animals of all ages depends on feeding conditions, time of year and other environmental factors. The stage of onset of sexual activity in animals differs sharply from all periods of growth and development. It has been revealed that timely mating of animals, on the contrary, naturally lengthens the maturation period and makes it possible to breed larger animals and obtain greater productivity in the future. For normal processes of growth and development in the body of animals, it is necessary to improve the external environmental conditions and, above all, the conditions of feeding, housing and care. It is necessary to build a breeding business in an exemplary manner and thus increase the weight and milk and meat productivity of livestock.

Lactate Concentration and Its Changes in the Blood of 3-month-old Rabbits

It was performed on 3-month-old rabbits, which were exposed to acute (20 minutes) and severe form of exogenous hypoxia (forced breathing in a nitrogen atmosphere with 5% O2 in pressure chamber conditions). Immediately after that (in the 1st, 3rd and 6th hours), the amount of lactate in their blood was determined. First, we studied the normal level of lactate in the blood of 3-month-old guinea pigs, then we subjected them to a hypoxia load and determined lactate. This series of experiments conducted by us allows us to get an idea of how blood lactate levels initially change in 3-month-old rabbits that have not yet reached sexual maturity under normal conditions and during the effects of various severe loads (such as acute hypoxia, long-term immobilization, and fast running). Here, we want to mention once again about lactate that this simple carbohydrate metabolite, along with other simple carbohydrates such as pyruvate (pyruvic acid) in the human and animal body, when there is a lack of oxygen in its cells, the glycolysis process (anaerobic decomposition of glucose) is carried out even faster, is an indicator of metabolic and functional importance that allows us to make judgments about its end products and at the same time its intensity and duration. As a final product of glycolysis, lactate accumulated in cells and tissues causes a change in the buffer systems there, an increase in the activity of the acidic range of pH (acidosis) and other negative reactions. An increase in the amount of lactate in the blood is considered a relatively weak toxic factor for the body. From this point of view, lactate is one of the harmful metabolites of the body. But studies have shown that the lactate formed as a result of glycolysis and other carbohydrate exchange processes is also used for the resynthesis (resynthesis) of sugars that are energetically useful for the body, that is, the body uses it as an energetic substrate. Therefore, the pronounced change in the level of lactate in the blood can also be considered as a kind of adaptive-defense reaction.

Urban Parks and Acceptable Animal Bodies

Urban Parks and Acceptable Animal Bodies

Assessment of the biological effect of natural silicon when entering the body of experimental animals with drinking water. Part 1

Introduction. The biological effect of natural silicon (Si) and its hygienic regulation in drinking water still remain subjects of controversy in the scientific literature and in the practice of providing favourable conditions for water use by the population. In this regard, an assessment was made of the effect of natural silicon contained in drinking water in concentrations close to the maximum permissible concentration on the body of laboratory animals.
 Materials and methods. Drinking waters containing natural Si at MPC levels in water were studied, their effect was assessed in a three-month experiment on 80 white outbred male rats. Observations were made of the general condition, appearance of the animals, body weight dynamics, water consumption. 
 The morphological composition of whole blood was studied. Biochemical indices of the condition of internal organs, including the liver, were determined 
 in serum samples (the ALT and AST activity, the level of total protein and albumin), pancreas (α-amylase activity), kidneys (creatinine content), as well as lactate dehydrogenase activity as a nonspecific indicator of tissue damage.
 Results. In animals received 17±3.4 mg/L silicon with drinking water at a hardness of 2.25±0.47 mg-eq/L, a significant decrease in ALT activity was found on the 30th and 90th days of the experiment, AST – on 90th day, increase in the content of total protein and albumin in the serum on the 30th day of the experiment. Drinking water with silicon concentrations of 20.5±4.1 mg/L and hardness 3.55±0.76 mg-eq/L decreased ALT activity and serum creatinine concentration on the 30th day and increased amylase activity on the 90th day of the experiment. The animals had the highest water consumption and intake of silicon from drinking water over the first month of the experiment.
 Limitations. Limitations of the study are related to the short duration of observation and the small number of points for determining biochemical parameters over time.
 Conclusion. Consumption of drinking water containing natural silicon for 90 days led to changes in biochemical indices, largely indicating the positive effect of the element in concentrations of 17±3.4 mg/L and 20.5±4.1 mg/L on the body of laboratory animals.

Selenium suppressed growth of Ehrlich solid tumor and improved health of tumor-bearing mice.

Selenium (Se) is an important micronutritional biomolecule in cancer therapy. The current work evaluated the anticancer effect of Se and its ability to improve health of mice with solid Ehrlich carcinoma implanted subcutaneously. Four groups of five female BALB/c mice each were assembled. Ehrlich tumor cells were engrafted into two of them, either with or without Se therapy. The other groups served as control groups, either with or without Se treatment. Se treatment resulted in a notable decrease in both tumor volume and animal body mass in tumor-bearing mice. Treatment with Se markedly increased oxidative stress in tumor while ameliorating oxidative stress in sera of tumors-bearing mice. Similarly, treatment with Se resulted in downregulation of inflammatory cytokines (TNF-α and IL-6) while increasing IL-10 in serum of tumor-bearing mice. Conversely, selenium increased TNF- α and IL-6 and decreased IL-10 in tumor suggesting disruption of tumor immunity. The increased oxidative stress and inflammation in tumor tissue dysregulated cell cycle phases with increase apoptotic tumor cells population in G0/G1 phase. This is supported by the increased levels apoptotic regulating proteins (Bax and caspase-3 and P-53) while decreasing Bcl-2 in the tumor tissue. Treatment with Se also resulted in increased comet parameters indicating DNA damage of tumor cells. Histopathological examination revealed a significant decrease in a number of neoplastic cells within tumor of mice that treated with Se. In conclusion, these findings suggest that Se therapy significantly suppressed solid tumor proliferation and growth while mitigating the health status of tumor-bearing mice.

Pathological characteristic of the eye changes within dogs ophthalmopathy associated with paraneoplastic syndrome

Ophthalmopathy associated with parneoplastic syndrome is a poorly studied pathology of the dogs eye. They occur as a result of the malignant neoplasm’s systematic exposure on the animal's body. They appear in the form of non-specific clinical signs. Main objective of the study – to study the pathomorphological changes in the dog’s eyes with malignant neo-plasms. The material for the study were 30 eyeballs enucleated in dogs in the terminal stage of cancer. Autopsy material obtained from animals was subjected to standard histological processing. As a result of histological studies, pathomorphological changes in eyeball structures associated with Patnaik G2 and G3 mastocytomas (28.6% of cases), liver and kidney carcinomas (28.6%), lymphoma (28.6%) and spleen hemangiosarcoma (14.2%) were revealed in 46.7% of cases. The main histopathological changes were thickening of the iris due to inflammatory cellular infiltration (57.1%) and vasodilation of blood vessels (42.9%), the presence of fibrinous hemorrhagic exudate in the anterior chamber of the eye (28.6%), edema and inflammatory infiltration of the vascular membrane proper (42.9%), thinning, loss of layering and weak retinal atrophy (42.9%). The revealed histological changes indicate the predominant involvement in the pathological process of the vascular membrane of the eye with a violation of the hematophthalmic barrier, as the main pathogenetic mechanism for the development of endogenous uveitis against the background of malignant neoplasm in the animal body.

MALASSEZIA FUNGAL DERMATITIS AND RHIPICEPHALUS SANGUINEUS TICK INFESTATION IN MIXED BREED DOGS

Dermatitis is the most common skin health problem in dogs whose causative agents are parasitic, bacterial, fungal and viral infections. The purpose of this paper is to diagnose and determine the success of dermatitis therapy due to Malassezia spp fungal infection and Rhipicephalus sanguineus tick infestation. A 7-month-old mixed breed dog weighing 4.5 kg was examined with complaints of itching and hair baldness. Clinical examination revealed alopecia on the face, chin, body and legs, scale on the head, papules on the abdomen, erythema on the cranial and caudal extremities with oily, rancid skin and Rhipicephalus sanguineus ticks on the hind legs. The results of cytological examination of skin taken by impression smear found the presence of Malassezia fungal agents. The results of the examination using the antibody kit showed negative results. Routine hematology examination showed the case dog had lymphocytosis and hyperchromic macrocytic anemia. Based on the history, physical examination, and supporting examination, the case dog was diagnosed with Malassezia fungal dermatitis and tick infestation with a prognosis of fausta.The therapy given was oral itraconazole, loratadine, fish oil, sebasol shampoo and multivitamin (sakatonic active). The results of therapy observed on day 14 showed that the condition of the case dog began to improve, characterized by a reduced scratching reaction, reduced scale, erythema, and papules, and superficial skin that was initially oily and rancid smelling had also begun to decrease.  The case dog's hair has grown on the head, abdomen and legs. Therefore, to avoid recurrent infections, owners need to avoid keeping the animal in a humid place and keep the animal's body clean as favorable conditions can trigger recurrent events, as well as reduce direct contact with stray dogs to prevent ticks.

Study of Tildox AVZ effect on rat blood parameters in a subchronic experiment

The purpose of our research was to study the effect of Tildox AVZ on rat blood counts in a subchronic experiment. Tildox is a complex antibiotic in powder form. One gram of drug contains doxycycline hyclate – 100 mg and tylosin tartrate - 100 mg as active ingredients, sodium benzoate and sucrose as auxiliary substances. The experiment lasted 90 days. The study was conducted on 150 Wistar rats (75 males and 75 females). The body weight of the animals was 190-230 g. During the experiment 5 groups of 30 animals (15 males and 15 females) were formed, three of them were experimental groups, one - control and one – intact. Of the 150 rats involved in the experiment after its completion 100 individuals (50 males and 50 females) were killed for pathomorphological studies of internal organs.To identify the ability to recover and the appearance of resistance to toxic effects the remaining 50 animals (5 males and 5 females from each group) were monitored for 28 days after the experiment.An aqueous solution of the test sample of the drug was administered daily intragastrically using an atraumatic metal probe. The drug was administered to the first experimental group at a dose of 70 mg / kg, the second group - 265 mg / kg, the third group - at a dose of 1000 mg / kg of animal body weight. The control group was in equal conditions and received intragastric saline solution. Intact animals served as a reference. It was found that Tildox AVZ administered intragastrically at doses of 265 and 1000 mg / kg 1 time per day for 90 days has an effect on clinical and biochemical blood parameters. The drug administered at a dose of 70 mg / kg does not cause changes in blood parameters. However when examining the blood of rats 28 days after the recovery period there were no significant changes in clinical and biochemical parameters compared with the control and intact groups.From this it can be concluded that all blood changes had a dose-dependent effect and after stopping the use of the drug the body recovered which indicates the reversibility of the caused shifts in the blood picture.

Protective effects of Moringa oleifera against acetic acid-induced colitis in rat: Inflammatory mediators’ inhibition and preservation the colon morphohistology

Moringa oleifera decoction is used in folk medicine to alleviate various gastrointestinal ailments and has been shown in several studies to exhibit diverse biological activities: antioxidant, anti-inflammatory, and myorelaxant on intestine strips. However, its protective effect on pro-inflammatory cytokines in colitis remains to be ascertained. We investigated Moringa oleifera leaf aqueous extract activities on a rat model of acetic acid-induced colitis. Thirty-six rats were divided in random into six groups, and received either distilled water (10 mL/kg), the aqueous leaf extract of Moringa oleifera (25, 50, and 100 mg/kg) or the reference drug Loperamide (5 mg/kg) for 20 days. Animal body weight and food intake were followed-up. Colitis was induced on day 15, and daily stool weight was followed up. Animals were sacrificed (day 21), serum concentrations of pro-inflammatory cytokines MPO, IL-6, and TNF-α were evaluated, and the colon and liver preserved for histological examinations. After one-week pre-treatment, a significant (P < 0.05) weight increase was observed. At day 20, the extract significantly (P < 0.01) prevented weight loss. Food intake decreased by 36.33% in the colitis control, and by 4.34% in the extract (100 mg/kg) treated animals (P < 0.01). Moringa oleifera (100 mg/kg, day 20) decreased stool weights to 4.75±0.3 g (P < 0.05). The extract decreased (P < 0.01) MPO level. At 100 mg/kg, it significantly (P < 0.05) reduced IL-6 –0.58 ± 0.14 against 0.85 ± 0.00 ng/L in the colitis control. At the same dose, TNF-α levels decreased to 417.96 ± 0.17 ng/L (P < 0.001). Moringa oleifera alleviated colitis symptoms by reducing stool emission, alleviating inflammation, and preserving colon and liver cytoarchitecture.

Arterial highways of the foot of the American raccoon (Procyon lotor)

The vascular system is one of the integrating systems of the human and animal body. The course and branching of the vessels of various areas of the body follows general patterns. However, the variability of the vascular bed is quite high even within a single species of animal. The lack of data on both the topography and the peculiarities of arterial branching makes it difficult to carry out diagnostic and therapeutic manipulations. In addition, the data obtained during the study significantly enrich the available information on the comparative anatomy of the vascular bed. The purpose of the study is to study the main arterial arteries of the American raccoon foot area, to give them a morphometric characteristic. The research was carried out on 4 corpses of an American raccoon, delivered to the Department of Animal Anatomy of the St. Petersburg State University of Veterinary Medicine from veterinary clinics. Classical methods were chosen as research methods: fine anatomical dissection, morphometry, photographing and vasorentgenography. During the study, it was found that two arterial highways are distinguished on the foot of the American raccoon. The dorsal trunk is represented by the dorsal artery of the foot, the plantar trunk is represented by the middle plantar artery. The dorsal artery of the foot passes into the arc artery – the first anastomosis that unites the vessels of the two highways. The middle plantar artery gives rise to the second and third plantar metatarsal arteries and participates in the formation of a deep plantar arch, which is the second major anastomosis of the foot area. Axial and non-axial finger arteries also form collateral blood flow. Analyzing the obtained morphometric data of arterial highways, it follows to conclude that the plantar surface highways have the largest diameter. Thus, the diameter of the middle plantar artery is 1.72 times higher than the same parameter of the dorsal artery of the foot. The largest total diameter is characteristic of the arteries located in the metatarsal region, the smallest in the area of the metatarsal.

Hematological manifestations of paraneoplastic syndromes in dogs

Hematological paraneoplastic syndromes are common but poorly studied complications in veterinary oncology, manifested by a variety of hematological changes associated with the system effects of malignant neoplasms on the animal's body. The article presents the results of studying hematological paraneoplastic syndromes, the research involves 131 dogs with spontaneous malignant neoplasms. We used a comprehensive methodological approach, including clinical, instrumental, morphological and hematological research methods. During the morphological study we found out that carcinomas were diagnosed in 33.6% of cases in dogs, of which breast carcinoma was registered in 24.4%, in other cases lung, liver, kidney and abdominal lesions were noted. We also discovered: sarcomas in 22.1% of cases, including hemangiosarcoma of the spleen in 7.6%; and gastrointestinal lymphoma in 16.8% of cases and lymph nodes; in 16.8% of mastocytomas. in 4.6% of melanomas of the skin and mucous membranes; squamous cell carcinoma in 6.1% of cases. As a result of hematological studies, it was revealed that anemia syndrome (18.4%), lymphopenia (16.8%) and thrombocytopenia (15.4%) are the most frequent changes in clinical blood analysis in dogs with oncological diseases. Neutrophilic leukocytosis, leukopenia and thrombocytosis had an identical incidence, which was 12.2% of cases. Monocytosis and lymphocytosis were diagnosed less frequently, in 4.6% of cases. Leukogram changes were observed in 75.4% of cases: in 66.2%, an increase in the percentage of neutrophils was found, with a shift to the left in 38.5% of cases, a decrease in the percentage of lymphocytes in 18.5% of cases, monocytes in 6.2% of cases, eosinophils in 4.6% of cases. The frequency of hematological changes in the general clinical blood analysis of dogs depended on the histogenesis of the tumor. Hematological paraneoplastic syndromes can be used as an early marker of the oncological process, and they can also prevent timely prescription of treatment. Anemia and thrombocytopenia are absolute contraindications for chemotherapy and radiation therapy.

Threat to Human and Animal Health Caused by Mycotoxins and Masked Mycotoxins Occurring in Food and Feed

Fungi, and especially mold fungi, play a very important role in natural environments, as well as in the food industry for the production of many specialized products. However, many of them, under favorable conditions, are capable of producing toxic compounds called mycotoxins. Many of them have an adverse effect on the growth and development of all groups of organisms, including humans. Mycotoxins can appear in all food products and animal feed. Mycotoxins accumulated in food of plant and animal origin and consumed by humans can accumulate in various tissues and organs, which results in disorders in their functioning and may cause cancer. Plants have the ability to defend themselves against mycotoxins by neutralizing them as a result of the glycosidation process. Mycotoxins neutralized in this way are called “masked mycotoxins”, which are not toxic to plants, but after entering the body of animals or humans in the digestive tract, they return back to their original toxic form. Let’s appeal and spread knowledge about mycotoxins because they pose a constant threat to the health of people and farm animals.

Assessment of the physiological features of equine cardiohemodynamics by volumetric compression oscillometry

The cardiovascular system of humans and animals is a complex of organs that ensure the supply of all parts of the body (with a few exceptions) with necessary substances and remove waste products. It is the cardiovascular system that provides all parts of the body with the necessary oxygen and is the basis of life. Currently, research into the problems of maintaining an optimal level of well-being, assessing the functional state and improving the adaptive capabilities of animals continues to be relevant. A significant direction in this area is the study of the activity of the cardiovascular system (CVS), the parameters of which are characterized by high reactivity and an important role in the implementation of adaptive mechanisms of the animal body. Cardiovascular diseases remain the leading cause of death in most cases in both humans and animals. In many ways, the solution to this problem depends on timely and effective diagnosis of changes in the myocardium. The main goal of the research work is to establish indicators of cardiohemodynamic values and analyze them in horses. In our studies, to measure and further analyze indicators of central and peripheral hemodynamics, we used a hardware-software complex for non-invasive study of central hemodynamics using the method of volumetric compression oscillometry KAP TsGosm-“Globus” (oscillometric analyzer of blood circulation indicators). In horses, during the study, average numerical values of myocardial-hemodynamic parameters were obtained: different types of blood pressure, vascular characteristics and indicators of cardiac activity. Mathematical analysis of hemodynamic indicators established the values of cardiohemodynamic indicators. In this regard, it is advisable to include the assessment of these parameters in the basic set of methods for diagnosing heart diseases in horses. The study of the cardiovascular system is of great importance in veterinary treatment and prophylactic work.

Studying the acids and amino acid composition of horse chestnut flowers

Introduction. Currently, there is an increasing trend towards assessing the full spectrum of chemical compounds in plant materials. One of the first steps in the formation of secondary metabolites of complex structure, participating in biochemical processes in the plant body, which have their own effect on the human and animal body, are organic acids (OA) and amino acids (AA). However, due attention is not always given to these compounds when studying the component composition of plant raw materials.Aim. The purpose of the work was to study the profile and quantitative assessment of the content of organic acids and amino acids of Aesculus hippocastanum L. flowers.Materials and methods. The object of the study were horse chestnut flowers, collected during flowering in the Voronezh region in 2021 and dried by air-shade method. Quantitative assessment of the amount of TC in terms of malic acid and ascorbic acid was carried out titrimetrically according to the methods presented in the State Pharmacopoeia of the Russian Federation, XIV edition. The composition of the OA and AA profiles of horse chestnut flowers was determined using the capillary electrophoresis method ("Kapel®-105/105M", Group of Companies "Lumex", Russia).Results and discussion. The method of capillary electrophoresis revealed the presence of 12 compounds of the OA group in a total amount of 4.7 % (in terms of absolutely dry raw materials). The rationality of recalculating the content of the sum of OA to the major component – citric acid, which amounted to 1.65 ± 0.04 %, was shown. The content of ascorbic acid was low (0.033 ± 0.007) %. Using the TLC method, the presence of 5 zones of compounds belonging to the AA class was established in the aqueous extract of horse chestnut flowers. The quantitative content of the sum of free AA in terms of glutamine, determined by the spectrophotometric method, was 2.25 ± 0.07 % (n = 5). The presence of 17 AA was determined by capillary electrophoresis, which amounted to 7.13 %. Glutamic acid is present in the greatest quantity in horse chestnut flowers (1.19 %). Essential amino acids amounted to 2.27 %; leucine was present in the predominant amount (0.58 %).Conclusion. A study was carried out of the qualitative composition and quantitative content of organic acids and amino acids of horse chestnut flowers. The composition of the profile and the quantitative content of organic acids and amino acids have been established. It was revealed that citric acid prevails in the total organic acids; glutamic, aspartic acid, arginine and proline are found in greater quantities in the total amino acids.

Importance of Nano-Sized Feed Additives in Animal Nutrition

"Nano", which derives from the Latin word nanus and means dwarf, refers to a very small unit of measurement equal to one billionth of a meter. Nanotechnology, which deals with the manipulation of matter at the atomic and molecular level, has an application area in animal husbandry as well as in many fields. Nano-sized feed additives, which have come to the forefront in the livestock sector in recent years, have become an innovative application used to increase the nutritional value of feeds and optimize animal health and performance. Since these additives are nano-sized particles with increased specific surface area, they can have a positive effect on a number of factors such as digestibility, nutrient absorption, immune system, growth and development. Minerals in the form of nanoparticles used as feed additives can increase bioavailability by passing through the intestinal wall to body cells faster compared to larger particles. The nano level of the substance not only increases the productivity of animals, but also brings the potential to improve the functionality of feed molecules. Nano feed additives increase the digestion and absorption of feed, allowing animals to benefit from feed more effectively. However, there are several challenges associated with this approach. These include the potential for endotoxin production, reduced nutrient absorption due to interaction with natural nutrients, the possibility of nanoparticle accumulation in the animal body, health risks, ethical considerations, environmental concerns and some negative effects such as interference with natural nutrients that can be avoided by encapsulation. This article discusses recent studies on nano-sized feed additives that offer potential benefits in animal nutrition.

Impact of sub-acute exposure to nuvan on serum lipoproteins of catfish, Clarias batrachus and their potential effects indirectly on human health

Pesticides are frequently applied in agricultural land, public health programs, and maintenance of trees or plants and also used as preservatives for different food materials for a long time. But these pesticides cause a lot of negative health and environmental impacts which affects the sustainability of an ecosystem. Among the different pesticides organophosphorus pesticide mostly used in agricultural purposes to prevent the disease causing agents like insects, pests, parasite due to its less persistence in our environment. Synthetic pesticide like Nuvan accumulations in the tissues of animals produce cyto-toxicity and have severed health issues due to its persistent nature. When these pesticides intake by aquatic animals specially fishes, they can interfere with the food chain. When such contaminated fishes eaten by someone they can destroy multiple systems in the body of human and other livings. Changes in blood biochemical indices gives signs what is happening in body of animals after exposure of pesticides. Most edible and commercially valuable freshwater fish Clarias batrachus was used in this work for analyzed the hazardous nature of toxic pesticide nuvan on some biochemical parameters. When fish exposed to two sub-lethal levels of nuvan (0.0137 ml/L and 0.0274 ml/L) for 60 days showed that the pesticide induces changes in blood parameters. The results showed very highly significant (p&lt;0.001) increase in Low- density lipoprotein-cholesterol (LDL) and highly significant (p&lt;0.01) increase in Very low- density lipoprotein-cholesterol (VLDL) while very highly significant (p&lt;0.001) reduction in high density lipoprotein- cholesterol (HDL) were noted in treated groups compared with the control. This study implicates that adverse effect on lipoproteins due to synthetic pesticides have a negative health effect as cardiovascular risk status. Research of environmental pollution and its adverse effects on human and other animals including fish is remarkable which can leads the sustainability of our environment.