Abstract Photobiomodulation (PBM) is a non-invasive therapy based on non-ionizing radiation emitted from light-emitting diodes (LEDs) and low-power lasers. PBM begins with photon-photoacceptor interactions, which lead to the production of trigger molecules, which in turn lead to molecular, cellular, and systemic effects. PBM based on amber light has emerged as a promising therapy for various dermatological and inflammatory conditions. However, there are few studies evaluating the effects of amber light, alone or in combination with other lights, on tumor cells. Thus, the aim of this study was to evaluate the effects of amber LEDs and low-power infrared lasers on DNA repair gene expression in breast cancer cells. For this, human breast cancer MCF-7 and MDA-MB-231 cells were exposed to amber LED (617 nm, 1500 mW, 0.13 cm 2 , 11.5 W cm −2 , 135 J, 1040 J cm −2 , 90 s) and low-power infrared laser (830 nm, 150 mW, 0.13 cm 2 , 1.1 W cm −2 , 12.8 J, 104 J cm −2 , 90 s), total mRNA was extracted, cDNA was synthetized, and APTX, PCNA, and POL β gene expression were evaluated by reverse transcription quantitative polymerase chain reaction. The data suggest that exposure to amber LEDs and low-power infrared lasers, alone or in combination, does not alter APTX, PCNA, and POL β gene expression in MCF-7 and MDA-MB-231 cells. The results suggest that exposure to amber LEDs and low-power infrared lasers does not alter the gene expression involved in the base excision repair pathway in MCF-7 and MDA-MB-231 cells. Such results could be taken into account when PBM is considered for breast cancer patients.