Alternative live feeds for small and sensitive fish early life stages such as pikeperch (Sander lucioperca L., 1758) can improve the larval quantity, quality and performance in aquaculture. Therefore, this study evaluated the cyclopoid copepod Apocyclops panamensis (Marsh, 1913) as live feed for pikeperch larviculture from day 11 post hatch (dph) in two independent experiments. In both experiments, pikeperch larvae had the highest specific growth rate (SGR) when they fed on Brachionus plicatilis until dph 11 and A. panamensis until dph 16–18. SGR was related to a decrease in total fatty acids (FAs), saturated FAs and monounsaturated FAs in pikeperch larvae, indicating their use as energy for growth. Within the polyunsaturated FAs, docosahexaenoic acid (DHA) increased in larvae fed with A. panamensis and coincided with the highest SGR suggesting that DHA is accumulated in larvae as structural FA. Our study demonstrated a suitable pikeperch larval fatty acid composition for growth after feeding A. panamensis compared with Artemia sp. from dph 11 until dph 16 and previously fed with B. plicatilis. Moreover, it highlighted the importance of the dietary PUFAs in pikeperch rearing, specifically of linoleic acid (LA) from dph 4 until dph 11 and of DHA from dph 11 onwards.

Masting is a well-known phenomenon in forest science in which trees recurrently produce exceptional high seed amounts throughout their lifespan. Occurring simultaneously across wide regions, masting may impact carbon sequestration and carbon cycling of forest ecosystems. To identify masting-induced changes in tree canopies, remote sensing methods may offer a suitable tool, though so far only few studies have used RS methods to investigate the relationship between masting and tree-ring width (TRW). Therefore, we employed a regional network of 19 European beech sites along a precipitation gradient in north-eastern Germany, providing time series of TRW (1961–2017) and categorical masting data (1992–2020). Combined with leaf area index (LAI) from the medium-resolution satellite sensors SPOT-VGT/PROBA-V and MODIS, we assessed the relationship between all three parameters by correlation analysis. Furthermore, we identified common climatic drivers and spatial masting patterns along gradients of water availability. We found negative relationships for both TRW and LAI with masting and a positive correlation for TRW with LAI for SPOT-VGT/PROBA-V data. Contrary, MODIS LAI provided no significant relationships. Water availability did not impact masting occurrence or intensity. The climate correlations showed that masting and TRW are commonly driven by summer climate in June and July, while we found no correlations between climate and LAI. We conclude that medium-resolved SPOT-VGT/PROBA-V LAI is suitable to observe masting but not to estimate TRW. Nonetheless, the results should be thoroughly interpreted in the context of uncertainties of RS measurements and complex interactions of different environmental drivers.

Urban development along coastlines is accompanied by habitat fragmentation and loss of habitat connectivity, particularly affecting the habitat and nursery function of estuarine areas for migratory marine species. Constructed floating wetlands, deployed as ‘stepping stones’ along urban coastlines where natural wetlands are missing, offer the potential to partially replace lost ecosystem services. Originally developed for wastewater treatment, constructed floating wetlands are now used to improve landscape aesthetics or create habitats for aquatic fauna and birds. This study presents a toolkit to identify appropriate sites for stepping stones using open source data and open source software alone. The toolkit was used to identify 85 potential installation sites along the German Baltic Sea coast, a large proportion of which are located in protected areas offering synergies with nature conservation measures. Though the sites are often located in protected areas, the field investigation revealed that natural vegetation is largely absent from the estuaries near urban areas. Constructed floating wetlands can never replace ‘core areas’ in ecological networks, but they can serve as stepping stones improving habitat connectivity, especially for diadromous fish species such as the threatened European eel. To ensure not only structural connectivity, but also functional connectivity, restoration efforts at the land-sea interface must be holistic and include adequate hydrologic connectivity.

AbstractDetermination of sample size (the number of replications) is a key step in the design of an observational study or randomized experiment. Statistical procedures for this purpose are readily available. Their treatment in textbooks is often somewhat marginal, however, and frequently the focus is on just one particular method of inference (significance test, confidence interval). Here, we provide a unified review of approaches and explain their close interrelationships, emphasizing that all approaches rely on the standard error of the quantity of interest, most often a pairwise difference of two means. The focus is on methods that are easy to compute, even without a computer. Our main recommendation based on standard errors is summarized as what we call the 1-2-3 rule for a difference of two treatment means.

Angler satisfaction is a key consideration in the management of recreational fisheries. Anglers typically prefer high catch rates and large fish, but the importance of these catch outcomes for satisfaction may differ across angler types, target species, and other contextual conditions. We examined the relationships between catch outcomes and satisfaction using trip-level (n = 19,558) catch and harvest information from two fisheries with contrasting governance and cultural contexts within the same nation, a small club context of north-western Germany (Lower Saxony) and a regional context with largely open access in north-eastern Germany (Mecklenburg-Western Pomerania). Both fisheries are from the same eco-region and offer multi-species fisheries of a similar species mix (predominantly freshwater). Catch rate and size of fish were found to positively affect catch satisfaction in both social-ecological contexts. The catch rate-satisfaction relationship showed diminishing marginal returns (i.e., more catch is better, but the marginal benefits diminish as catch increases), and the maximum fish size-satisfaction relationship was positively exponential (i.e., larger maximum fish sizes make anglers increasingly more satisfied). Social-ecological context, trip context (e.g., season and previous catch outcomes) and angler specialization were all significant moderators of the importance of catch outcomes towards satisfaction with catch. Importantly, after controlling for catch outcomes and other contextual factors, anglers in the small-scale club context from north-western Germany (Lower Saxony) were, on average, more satisfied with their catch than anglers in a large-scale regional context from north-eastern Germany (Mecklenburg-Western Pomerania). These findings suggest that managers cannot expect anglers to be similarly satisfied at identical catch outcomes in different social-ecological contexts, even within the same nation. Managers may well be advised to manage for specific qualities of catch (e.g., regularity of catch and larger maximum size of fish) rather than attempting to manage for high catch rates alone as the latter might not contribute to more satisfied anglers after catch rate thresholds have been passed.

AbstractA robust assessment of the American eel (Anguilla rostrata) stock, required to guide conservation efforts, is challenged by the species’ vast range, high variability in demographic parameters and data inadequacies. Novel ideas and underutilised resources that may assist both analytic assessments and spatially oriented modelling include (1) species and environmental databases; (2) mining of data from scattered sources; (3) infilling of data gaps by spatial analysis; (4) age estimation from measurements of DNA methylation; evaluation of eel abundance by (5) larval, (6) glass‐bottom boat, (7) net enclosure and (8) eDNA surveys; (9) accounting for dam‐induced habitat increases in eel watercourse modelling; (10) spatially oriented modelling with and without temporal components; (11) geographically nested modelling of glass eel recruitment; (12) spawner per recruit modelling and (13) life cycle modelling to examine larval allocation effects. Eel biologists are too few to gather the required assessment data across all of the species’ range. Public posting of electrofishing and eDNA metabarcoding data sets and the use of machine learning techniques to comprehensively inventory small dams will help meet some data needs. These approaches address only a small proportion of the assessment challenges that face American eels. Worldwide collaboration amongst Anguilla scientists is a key enabler of progress towards stock assessment goals.

Pikeperch (Sander lucioperca) is a freshwater fish that has become increasingly popular as a food fish. Within this study, the influence of the spawning process on meat quality was investigated. For this purpose, adult pikeperch was examined directly before and after spawning, and compared regarding physical meat parameters and lipid composition. The results indicate that after spawning, the values of the pH, the electrical conductivity and the yellowness value of fillet were significantly higher than those of the animals sampled before spawning. Analysis of the sum of the total lipid content indicates no differences before and after closed season, but differences in the fatty acid profile were present. Despite significant lower MUFA concentrations, the EPA and DHA showed unaffected high contents. Therefore, the fish muscle indicated an equivalent meat quality. Nevertheless, the significant changes of some physical meat quality parameters after spawning season could have a particular impact on the shelf life and storage of the pikeperch fillet, highlighting the need for further research.

BackgroundConsidering wild inter-populational phenotypic differentiation can facilitate domestication and subsequent production of new species. However, comparing all populations across a species range to identify those exhibiting suitable key traits for aquaculture (KTA; i.e. important for domestication and subsequent production) expressions is not feasible. Therefore, proxies highlighting inter-populational divergences in KTA are needed. The use of such proxies would allow to identify, prior to bioassays, the wild population pairs which are likely to present differentiations in KTA expressions in aquaculture conditions. Here, we assessed the relevance of three alternative proxies: (i) genetic distance, (ii) habitat divergence, and (iii) geographic/hydrologic distances. We performed this evaluation on seven allopatric populations of Perca fluviatilis for which divergences in KTA had already been shown.ResultsWe showed differences in the correlation degree between the alternative proxy-based and KTA-based distance matrices, with the genetic proxy being correlated to the highest number of KTA. However, no proxy was correlated to all inter-populational divergences in KTA.ConclusionFor future domestication trials, we suggest using a multi-proxy assessment along with a prioritisation strategy to identify population pairs which are of interest for further evaluation in bioassays.

After being proposed by Lloret et al. in 2011, the resilience components resistance, recovery, resilience and relative resilience became highly popular for studying tree growth responses to disturbances and extreme weather events. As the ‘Lloret indices’, however, have conceptual limitations, various additional indices to characterize tree resilience have been discussed since then, namely: recovery period, total growth reduction, average recovery rate, and average growth reduction. In pointRes 2.0, a major update of the R-package pointRes, these new indices have now been implemented. Besides convenient functions for calculating and plotting resilience indices, two new functions for calculating pointer years using (i) the interval trend method, and (ii) a 'z-transformation of site chronology'-approach have been included as well. This technical note illustrates pointRes' new functionalities.