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Aportes a la morfología de semillas de Hibiscus cannabinus L. y ajuste de la prueba de tetrazolio para estimar viabilidad y vigor

Hibiscus cannabinus L. is an annual species cultivated for its fibers. The morphological description of the seeds of the cultivars ‘Endora’ and ‘Tainung' 1’ was broadened, a tetrazolium test procedure was adapted and adjusted, and the embryos were classified according to their viability and vigor. Cells from the seed coat layers participate in the seed coloration and ornamentation. The imbibition cap and the isthmus were identified, and their function wasanalyzed. Lipids and proteins were recognized in the mesophyll of cotyledons and the absence of areoles in the epidermis. Using the tetrazolium test, staining patterns were established to classify the seeds according to their viability. Both cultivars presented acceptable viability values. The germination test supported the proposed methodology for tetrazolium. Novel contributions are made to seed morphology, and structure-function relationships for water intake are proposed. The seeds of both cultivars share the morphological characteristics that are fundamental to the establishment of evaluation criteria in the tetrazolium biochemical test. The adapted tetrazolium viability test protocol allowed the seeds classification as either viable into three categories (high, medium, and low vigor) and non-viable seeds.

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Assessment of land use change in the dryland agricultural region of Córdoba, Argentina, between 2000 and 2020 based on NDVI data

The dryland region of Córdoba province experienced a strong increase in agricultural land-use in the 21st century. Between years 2000 and 2020 the temporal variation of land-use measures derived from the seasonal variation curve of the Normalized Difference Vegetation Index (NDVI) was analyzed. In eleven departments of the region, the following NDVI measurements wereobtained for each crop cycle from September to April: minimum value (NDVIn), maximum value (NDVIx), amplitude (NDVIa=NDVIx-NDVIn) and mean value (NDVIm). The sowing percentage per department was analyzed spatially and temporally, as well as the land use indicators. Both NDVIn and NDVIx are related to the sowing area per department, determining a negative correlation (-0.36) for NDVIn and a positive one (0.596) for NDVIx. The positive correlation with NDVIa (0.569) is considered directly linked to the agricultural land use. The seasonal variation of the NDVI showed changes over time, which werecompatible with the increase in agricultural activity in the region. Although the increase in agricultural land use was noticeable through both the decrease in NDVIn and the increase in NDVIx, along with a general trend towards rising NDVIa values, the variation was more apparent in those departments where agricultural activity increased to a greater proportion.

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Selección de cepas bacterianas con capacidad antifúngica contra fitopatógenos de alfalfa para constituir un consorcio bacteriano

Alfalfa breeding programs require environmentally friendly alternatives to improve plant growth and health. Plant Growth Promoting Rhizobacteria (PGPR) and endophytic bacteria offer a biological solution as they can inhibitphytopathogenic fungi by producing antifungal compounds or enzymes. In Argentina, alfalfa is primarily affected by Colletotrichum trifolii (Ct) and Phytophthora megasperma (Pm). The objectives of this study were to select bacterial isolates with antifungal inhibition against Ct and Pm and identify a biocontroller isolate compatible with the rhizobia INTA5 has and with low-N2O emissions, to constitute an eco-friendly bacterial consortium. Ten isolates demonstrated antifungal inhibition, with mean Inhibition Percentage (IP) values between 41.74-91.28 for Ct and 56.23-77.59 for Pm compared to the control. Among these isolates, B2, B4, SC6, and MN1 showed the highest inhibition performance and were selected to evaluate their compatibility with INTA5. Only B4 exhibited compatibility, which was further evaluated against Ct in an experiment with alfalfa seedlings under controlled conditions. The alfalfaseedlings treated with B4 increased the plant survival number against Ct. The availability of a bacterial consortium that promotes biocontrol and biological nitrogen fixation represents the preferred strategy for alfalfa breeding programs.

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