The initial lockdown phase of the COVID-19 pandemic presented an unfortunate opportunity to observe how abrupt, large-scale changes in traffic volume can reduce greenhouse gas emissions. This study explores how carbon dioxide (CO2) emissions from Virginia’s transportation sector may have been affected by the changes in activity stemming from COVID-19 to inform more carbon-neutral policies as the state recovers from the economic downfall. Emission savings were calculated by multiplying the percent change from 2019 to 2020 in traffic volume from the Virginia Department of Transportation with the business-as-usual 2020 U.S. Environmental Protection Agency estimate of CO2 emissions for Virginia’s transportation sector. We estimate Virginia’s 2020 COVID-19 transportation CO2 emissions reduction is around 15.0% (14.2 to 15.7%), with reduced passenger vehicle traffic making up the bulk of the inferred reduction. This study highlights the utility of reimagining our current transportation sector as a way to implement sustainable, state-level carbon reduction policies, such as the Clean Car Standards.

Wetland disturbance from motorized vehicle use is a growing concern across the Appalachian coalfields of southwestern Virginia and portions of adjacent states, particularly as both extractive industries and outdoor recreation development expand in regional communities. However, few attempts have been made in this region or elsewhere to adapt approaches that can assist researchers and land managers in remotely identifying and monitoring wetland habitats disturbed by motorized vehicle use. A comparative analysis of wetlands impacted and unimpacted by off-road vehicle activity at a public recreation area in Tazewell County, Virginia was conducted to determine if and how a common, satellite-derived index of vegetation health, normalized difference vegetation index (NDVI), can remotely detect wetland disturbance. NDVI values were consistently lower in wetlands impacted by several years of off-road vehicle use when compared to adjacent, unimpacted sites, with statistically-significant NDVI coldspots growing in size in impacted wetlands across the same time period. While considerations exist related to the resolution of data sources and the identification of specific modes of disturbance, NDVI and associated spatial analysis tools may provide a simple and cost-effective way for researchers and land managers to remotely monitor rates of wetland disturbance across mountainous portions of the eastern United States.

The upper Roanoke River has 11 species of Catostomidae including Moxostoma ariommum, Bigeye Jumprock; Moxostoma cervinum, Blacktip Jumprock; and Thoburnia rhothoeca, Torrent Sucker. Resource partitioning appears to be a key component of maintaining diverse fish assemblages with habitat and food partitioning cited as especially important in communities containing members of the same family. The diets of these species have been documented in previous work revealing only modest differences among them. Snorkeling observations and subsequent quantification of microhabitat were conducted to illuminate habitat partitioning among these morphologically and ecologically similar species. Thoburnia rhothoeca inhabited the shallowest, fastest water, over the smallest substrate, and Moxostoma ariommum inhabited the deepest, slowest water, over the largest substrate, with M. cervinum intermediate for all habitat variables. In an effort to correlate morphological adaptations to these different microhabitats, 22 body measurements were included in a Principal Component Analysis revealing a bigger eye for M. ariommum and more fusiform bodies for T. rhothoeca and M. cervinum consistent with findings in other species inhabiting faster waters. Other correlations among morphology and microhabitat were less clear.

Microplastics are pollutants of concern in waterways and oceans for their persistence and impact on aquatic life and food webs. This study demonstrates a low-cost land-based sampling method to assess the extent of microplastic pollution found in Four Mile Run, an urban stream in Northern Virginia. Microplastic particle counts in environmental and treated wastewater ranged from 0.01-0.24 particles L-1 (mean 0.08 particles L-1) and from 2 to 446 µg L-1 (mean 70 µg L-1), with fibers found to be the most common microplastic category. Treated wastewater effluent was found to be a significant source of microplastic pollution, though microplastics were also found upstream of any influence from wastewater or tide-borne materials. The sampling method proved effective for collecting and analyzing microplastic pollution, though the sample size of 100 L was deemed insufficient for reliable measurement of total mass of microplastics.

The objectives of this research are to determine the factors associated with the minimum, maximum, and average thermal emittance temperatures of wall murals in Richmond, Virginia, and make recommendations for color and location of wall murals. Data for 17 parameters were collected for 64 art murals in downtown Richmond, VA from 28 June 2018 – 19 July 2018. Date, time, solar exposure duration, solar elevation, latitude, longitude, air temperature (C), lux, color, solar cardinal minutes, cardinal direction, minimum, maximum, and average surface thermal emittance temperatures of mural face were recorded for each mural. We reject the hypothesis that minimum, maximum, and average thermal emittance temperatures of wall murals do not vary significantly with temporal, spatial, physical changes, and solar factors. Maximum, minimum, and average emittance temperatures of wall mural art varied significantly with the number of minutes that solar energy directly illuminating mural art for each cardinal direction, lux, solar elevation, total diurnal exposure, maximum mural color, and percent dark colors on murals. Minimum mural wall art temperatures (30.0-37.0 C) occurred on surfaces facing East, North, Northwest, and Northeast which also had the lowest lux values (8,867-14,231); highest temperatures (44.8-49.9 C) were recorded on walls facing South, Southeast, and West, which had the highest lux values (42,517-46,000). Based on results of this study and those investigating mitigation of the urban heat island effect with building materials and coatings on walls and roofs, we recommend a study that uses a systematic approach to locating wall mural art based on specific colors, paint composition, and wall materials to guide local building authorities owners, and artists to maximize albedo. Additionally, we recommend, where possible, mural art should be painted on vertical surfaces facing North, Northeast, and Northwest where lux values and thermal gain are the lowest of all possible cardinal directions. Such locations could accommodate the use of dark colors without significantly increasing the heat load in urban communities. And finally, where mural art is being considered for building walls facing South, Southeast, Southwest and West, we recommend that artists use a palette of primarily light colors with high reflective properties to maximize albedo, and minimize use of dark colors.

With an ever-increasing footprint, already topping three billion devices, smartphones have become a huge cybersecurity concern. The portability of smartphones makes them convenient for users to access and store personally identifiable information (PII); this also makes them a popular target for hackers. This survey paper shares practical insights derived from analyzing 16 real-life case studies that exemplify: the vulnerabilities that leave smartphones open to cybersecurity attacks; the mechanisms and attack vectors typically used to steal PII from smartphones; the potential impact of PII breaches upon all parties involved; and recommended defenses to help prevent future PII losses. The contribution of this research is recommending proactive measures to dramatically decrease the frequency of PII loss involving smartphones.

Agouti-related peptide is a 132-amino acid peptide associated with stimulating food intake in birds and mammals. The aim of the present study was to investigate the effect of AgRP in seven-day old Japanese quail. In Experiment 1, we tested 0.25, 0.5 and 1.0 nmol AgRP and found no effect on food or water intake over a three-hour period. In Experiment 2, we tested 0.25, 0.5 and 1.0 nmol AgRP and found no effect on food or water intake over 24 hours. In Experiment 3, we tested 0.0625 and 0.125 nmol AgRP and found no effect on food intake over a 24-hour duration, but found an increase in water intake 900 minutes following injection. In Experiment 4, we found an increase in food and water intake 900 minutes following injection in quail which received 1.5, but not 3.0, nmol AgRP. In Experiment 5, we found that AgRP had no effect on behaviors other than food intake. These results suggest that AgRP might have a stimulatory effect on food intake in Japanese quail.

Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from White’s Ferry, Virginia, to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to the P. cf. isothrix blooms in the study site, aquatic macroinvertebrate community assemblages were significantly different between areas with extensive benthic cyanobacterial growth compared to areas without cyanobacterial growth. Within the P. cf. isothrix mats, the percentage of pollution sensitive taxa was lower, and the percentage of pollution tolerant taxa was greater. These data suggest that P. cf. isothrix can act as an ecosystem disruptor through direct impacts to the aquatic macroinvertebrate abundance and community structure within this section of the freshwater, non-tidal Potomac River.

Most operating websites experience a cyber-attack at some point. Cross-site Scripting (XSS) attacks are cited as the top website risk. More than 60 percent of web applications are vulnerable to them, and they ultimately are responsible for over 30 percent of all web application attacks. XSS attacks are complicated, and they often are used in conjunction with social engineering techniques to cause even more damage. Although prevention techniques exist, hackers still find points of vulnerability to launch their attacks. This project explored what XSS attacks are, examples of popular attacks, and ways to detect and prevent them. Using knowledge gained and lessons-learned from analyzing prior XSS incidents, a simulation environment was built using XAMPP and VirtualBox. Four typical XSS attacks were launched in this virtual environment, and their potential to cause significant damage was measured and compared using the Common Vulnerability Scoring System (CVSS) Calculator. Recommendations are offered for approaches to impeding XSS attacks including solutions involving sanitizing data, whitelisting data, implementing a content security policy and statistical analysis tools.

Although the United States supports a considerable diversity of spiders, some aspects of spider habitat use and niche specialization are poorly documented. Specifically, little attention has been given to explore how urban development affects the diversity and abundance of arthropods. We sampled spiders along an outdoor – indoor habitat gradient at Longwood University to understand the impact of urbanization on species diversity and abundance. We found 50 taxa of spiders belonging to 43 genera and 16 families. Overall, the most abundant spider family across three sampling sites was Araneidae (orb-weavers; 18.2%) followed by Lycosidae (wolf spiders; 14.8%), Salticidae (jumping spiders; 13.6%) and Linyphiidae (sheetweb spiders; 12.5%). We found the highest species richness, spider abundance, and Shannon-Wiener diversity from Lancer Park (i.e. outdoors habitat), followed by the habitats associated with outside of the science center building (i.e. marginal habitat) and the lowest spider diversity inside the science building (i.e. indoors habitat). We also found a strong positive correlation between overall spider diversity and air temperature for outdoors and marginal habitats, but no correlation with relative humidity. Our study adds original knowledge about habitat use of spiders along an outdoor - indoor habitat gradient and arthropod use of indoor biome. More importantly, our study stresses the need for more extensive systematic studies to fully understand how spatial and temporal variation of arthropod diversity and abundance may be influenced by alterations of habitats by humans through urbanization.