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Statut biologique et ingérés en micronutriments chez des femmes et des jeunes enfants, Burkina Faso

Introduction La biofortification est une strategie prometteuse pour lutter contre les carences en micronutriments en pays defavorises. La mise au point de telles strategies necessite d’etablir la part des carences provenant d’ingeres trop faibles. Une etude a ete realisee dans ce but aupres d’enfants âges de 36 a 59 mois et leurs meres en milieu rural au Burkina Faso. Methodes Une enquete transversale sur echantillon aleatoire de 480 couples mere-enfant a ete effectuee en 2010. La mesure de la consommation alimentaire a ete faite par rappel des 24-heures avec repetition sur un sous-echantillon. Les ingeres ont ete exprimes par la probabilite d’adequation (PA) aux ingeres moyens recommandes pour chaque micronutriment (fer, zinc, vitamine A). Les biomarqueurs seriques des micronutriments ont ete doses chez 180 meres-enfants (diagnostic des carences par rapport aux seuils internationaux). Les relations entre biomarqueurs et ingeres ont ete etudiees par modeles de regression lineaire multiple. Resultats Les PA moyennes pour le fer, zinc et vitamine A etaient respectivement de 49 %, 87 % et 48 % parmi les femmes et de 61 %, 95 % et 58 % chez les enfants. Les prevalences de faibles ferritine, zinc et retinol seriques etaient respectivement de 4,0 %, 39,4 % et 12,0 % chez les femmes et de 1,5 %, 63,7 % et 24,8 % chez les enfants. En modele multivarie, les faibles ingeres n’apparaissaient pas lies au statut biologique, pour aucun des micronutriments consideres. Discussion Des biomarqueurs plus appropries des carences en micronutriments ainsi que des mesures plus precises des ingeres, notamment tenant compte du niveau d’absorption des micronutriments, sont necessaires pour etablir ces relations au niveau individuel.

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Antioxidative properties and ability of phenolic compounds of Myrtus communis leaves to counteract in vitro LDL and phospholipid aqueous dispersion oxidation.

Antioxidant activities of Myrtus communis leaf phenolic compounds (McPCs) were investigated on 2,2'-9-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS(+) •) and oxygen radical absorbance capacity (ORAC) tests or on oxidation of biological models, human low-density lipoprotein (LDL) and phospholipid aqueous dispersion (L-α-phosphatidylcholine stabilized by bile salts). Two extraction techniques, microwave-assisted (MAE) and conventional (CE), were used to isolate McPCs, producing similar results of phenolic compound content. ABTS(+) • assay showed clearly that myrtle extracts exhibited a stronger scavenging effect than butylated hydroxyanisole and α-tocopherol, with a slight advantage for myrtle CE extract. In ORAC assay, the both McPC extracts were similarly less effective than the pure compounds as caffeic acid and myricitrin (myricetin 3-O-rhamnoside) but stronger than butylated hydroxytoluene. Moreover, myrtle CE and MAE extracts, and myricitrin were able to inhibit similarly the production of conjugated dienes and to prolong the lag phase (Tlag) during Cu(2+)-induced LDL oxidation with a dose-response effect. The cryo-electron microscopy observations on studied phospholipid dispersion stabilized by bile salts (BS) revealed the presence of bilayer vesicles and micelles. In 2,2'-azobis (2-amidinopropane) hydrochloride-induced phospholipid/BS oxidation, myrtle CE and MAE extracts gave similar effects to α-tocopherol and caffeic acid but myricitrin showed a higher protective effect than myrtle extracts. We showed also that no synergic or additive effect between α-tocopherol and myrtle extracts or caffeic acid in α-tocopherol-enriched phospholipid/BS dispersion, but myricitrin showed an additive effect and thus promoted the total antioxidant activity. These data showed that myrtle extract could be used as potential natural antioxidants, food stabilizers, or natural health products. We show that microwave-assisted extraction could be an alternative method for plant phenolic compound recovery allowing important gain in time extraction.We report inhibition of low-density lipoprotein oxidation in vitro initiated by Cu(2+) ions. We report that myrtle extract may be a source of natural antioxidants to counteract phospholipid peroxidation as well as α-tocopherol.

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Factors associated with growth patterns from birth to 18 months in a Beninese cohort of children

The aim of this study was to analyze factors influencing the growth pattern of children from birth to 18 months. A longitudinal prospective study was conducted in three maternity wards in Southern Benin. Inclusion took place between June 2007 and July 2008; children were followed-up until 18 months of age. Height-for-age and weight-for-height Z-scores were computed using the newborn's anthropometric measurements taken at delivery, every month up to 6 months and then quarterly. Infant and young child feeding (IYCF) practices and malarial morbidity were recorded. Gestational age was estimated using the Ballard method; William's sex-specific reference curve of birth weight-for-gestational-age was used to determine intrauterine growth retardation (IUGR). Analyses were performed on 520 children using a linear mixed model. Low birth weight (coef=−0.43; p=0.002), IUGR (coef=−0.49; p<0.001), maternal short stature (coef=−0.25; p=0.001) and maternal low weight status (coef=−0.19; p=0.006) were significantly associated with growth impairment. Only LBW (coef=−0.28; p=0.05) and maternal low weight status (coef=−0.23; p=0.004) were associated with wasting. A good IYCF score was positively associated with weight gain (coef=0.14; p<0.001) whereas we found a paradoxical association with length (coef=−0.18; p<0.001). Malaria morbidity was not associated with growth. LBW, IUGR and maternal low weight status and height were important determinants of children's growth. These results reinforce and justify continuing public health initiatives to fight IUGR and LBW and break the intergenerational cycle of malnutrition.

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Potential Health Implication of <i>in Vitro</i> Human Low-Density Lipoprotein–Vitamin E Oxidation Modulation by Polyphenols Derived from Côte d’Ivoire’s Oil Palm Species

Antioxidant activities of polyphenolic compounds extracted (PPEs) from ripe fruits of oil palms are investigated by studying their in vitro effects on human low-density lipoprotein (LDL) oxidation. Four oil palm species ( Elaeis guineensis ) are issued from the National Centre of Agronomic Research of Côte d'Ivoire, of which two are parental varieties (HP1 and HP2), while the other two are crossing varieties (HP3 and HP4). The main identified compounds were rutin (HP3 and HP4) and caffeic and chlorogenic (5-caffeoyl quinic) acids (HP1, HP3, and HP4). The highest total phenolic content was found for HP4, while it was significantly lower for HP2. Antioxidative effects were monitored by Cu(2+)- or 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH)-induced generation of conjugated dienes (lag time and oxidation rate). The highest PPE specific antioxidant activity (SAA) values were obtained with crossing varieties (HP3 and HP4) in the copper-oxidation assay. In the AAPH-oxidation assay, SAA values were comparable for all four varieties. PPEs were effective at preventing LDL-vitamin E depletion in vitro. They could exert direct beneficial antioxidant effects on vitamin E and other antioxidants contained in food and beverages in vivo, within the gastrointestinal (GI) tract. These data could also be of particular importance for a healthier nutrition or the management of chronic diseases by a polyphenol-rich diet.

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