To investigate the low-speed impact response of the BR1200HS steel and AA6082 aluminum alloy self-piercing riveted (SPR) joint, low-speed impact tests with impact energies of 2.5 J, 5.0 J, 7.5 J, 10.0 J, 12.5 J, 15.0 J, 20.0 J, and 30.0 J were conducted utilizing a drop hammer impact tester. The results show that with the increased impact energy, the visual breakages of the SPR joints become more severe. The maximum impact energy the joints can sustain ranges from 10.0 J to 12.5 J. When the impact energy is less than 12.5 J, the contact force/energy–time curves show similar variations. Moreover, as the impact energy increases, total uptake energy value (Et), maximum uptake energy value (Ef), and maximum contact force (Fm) of the joints increase gradually. The low-speed impact energy has little effect on the maximum static tensile force of the impacted joints. However, the residual energy values decrease with the increase in impact energy. The tensile failure form of the joints is the pulling out of the rivets from the lower plates, and the low-speed impacts have no significant effect on the tensile failure form of the joints.

To observe the effect of meridian sinew releasing technique on moxibustion sensation of heat-sensitive moxibustion in patients with knee osteoarthritis (KOA).A total of 60 patients with KOA were randomly divided into an observation group and a control group, 30 cases each group. In the observation group, on the basis of the meridian sinew releasing technique, moxibustion sensation exploration method was applied at Dubi (ST 35) area on the affected side. In the control group, moxibustion sensation exploration method was applied at Dubi (ST 35) area on the affected side. The meridian sinew releasing technique was performed for 20 min each time, the moxibustion sensation exploration method was performed for 60 min each time, once a day for 3 days. The excitation rate, latency, duration time and intensity value of moxibustion sensation of heat-sensitive moxibustion were recorded on the 1st, 2nd and 3rd days of exploration in the two groups.The excitation rate on the 3rd day of exploration and total excitation rate in the observation group were higher than the control group (P<0.05). On the 1st, 2nd and 3rd days of exploration, the latency of moxibustion sensation of heat-sensitive moxibustion in the observation group was shorter than the control group (P<0.05), the duration time was longer than the control group (P<0.05), and the intensity value was higher than the control group (P<0.05).Meridian sinew releasing technique could improve the excitation rate of moxibustion sensation of heat-sensitive moxibustion in patients with KOA, shorten the latency, prolong the duration time, and improve the intensity value.目的：观察经筋手法松解术对膝关节骨关节炎（KOA）患者热敏灸感的影响。方法：将60例KOA患者随机分为观察组和对照组，每组30例。观察组在经筋手法松解术的基础上，于患侧犊鼻穴区行灸感探查法；对照组直接于患侧犊鼻穴区行灸感探查法。经筋手法松解术每次20 min，灸感探查法每次60 min，均每日1次，连续3 d。记录两组患者探查第1、2、3天热敏灸感激发率、潜伏期、持续时间、强度值。结果：观察组探查第3天热敏灸感激发率及总激发率高于对照组（P<0.05）。探查第1、2、3天，观察组热敏灸感潜伏期短于对照组（P<0.05），热敏灸感持续时间长于对照组（P<0.05），热敏灸感强度值高于对照组（P<0.05）。结论：经筋手法松解术可提高KOA患者热敏灸感激发率，缩短潜伏期，延长持续时间，提高强度值。.

Halichlorine and pinnaic acid are structurally related natural alkaloids isolated from different marine organisms. These two marine alkaloids bearing a 6-azaspiro[4.5]decane skeleton demonstrate a wide range of biological effects. It is this kind of unique structure and potentially valuable biological activity that have prompted strong synthetic interest, making it a research focus in recent years. Since the first total synthesis of halichlorine and pinnaic acid completed by Danishefsky's group, many groups have reported their outstanding synthesis methods especially the asymmetric synthesis strategies. This review summarizes the asymmetric synthesis strategies of halichlorine and pinnaic acid using a 6-azaspiro[4.5]decane skeleton as the key intermediate, which can provide some guidance for related work.

Shaoyao-Gancao Decoction (SGD) is a well-known classic traditional Chinese medicine (TCM) with antispasmodic, anti-inflammatory, and analgesic effects. This preparation has been widely used to treat spasticity diseases in the clinic. To date, the material basis of SGD remains unclear, and the spectrum-effect correlation of its antispasmodic activity has not been reported yet. In this study, high-performance liquid chromatography (HPLC) was used to establish the fingerprint and determine the multiple components of SGD. The common peaks of fingerprints were evaluated by the similarity with the chromatographic fingerprints of the TCM. Meanwhile, the multiple components were quantified and analysed using the heatmap and box size analysis. Furthermore, data on the antispasmodic effect were extracted through in vitro smooth muscle contraction assay. Grey relational analysis combined with partial least square regression was used to study the spectrum-effect correlation of SGD. Finally, the potential antispasmolytic components were validated using an isolated tissue experiment. The HPLC fingerprint was established, and 20 common peaks were identified. The similarities of 15 batches of SGD were all above 0.965. The HPLC method for simultaneous determination of the multiple components was accurate and reliable. The contents of albiflorin, paeoniflorin, liquiritin, and glycyrrhizic acid were higher than the other components in SGD. The heatmap and box size also showed that X3 (albiflorin), X4 (paeoniflorin), X5 (liquiritin), X11 (liquirtigenin), and X16 (glycyrrhizic acid) could be used as quality indicators in the further establishment of quality standards. The spectrum-effect correlation results indicated that X4, X11, and X16 were highly correlated with antispasmolytic activity. Verification tests showed that paeoniflorin (11.7-29.25 μg/mL) and liquirtigenin (17.19-28.65 μg/mL) could significantly reduce the maximum contractile (P < 0.01). These compounds exerted concentration-dependent spasmolytic effects with the inhibitory response for acetylcholine (Ach)-evoked contraction. Thus, SGD had a significant antispasmodic effect, which resulted from the synergistic activity of its multiple components. These findings can be used for the pharmacodynamics study of SGD and are of great significance for the determination of quality markers and quality control.

Abstract The translation model is a useful tool for simulating non-Gaussian wind speed time series, it employs marginal transformation to fit the distribution function of wind speed, and deploys a Gaussian process with a specified autocorrelation function (ACF) ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ to match the ACF of wind speed. However, determining ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ requires solving an integral equation, which is not computationally convenient. In this paper, an easy-to-use method is presented for determining ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ . Based on Spearman’s rank correlation coefficient, a rank ACF is proposed to characterize the time-dependent behavior of wind speed time series, whereby ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ can be specified by an analytical formula. In the case that only discrete values of ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ are available, a Laguerre polynomial based model is developed to fit ρ Z ( τ ) ${\rho }_{Z}\left(\tau \right)$ . Furthermore, if the distribution function of wind data is unknown, the kappa distribution is introduced to fit the quantile function of wind speed. Finally, a case study is conducted to check the proposed method, the results indicate that the rank correlation based translation model can yield a satisfactory representation for statistical features of wind speed time series.

Automotive light-weighting has become an important direction for the development of the automotive industry, and the light-weighting materials are inevitable to be joined. Self-piercing riveting (SPR) is a kind of new process to joint various materials. The SPR and SPR-bonded (SPR-A) joints of AA6061 and DP590 sheets were prepared. The mechanical properties of SPR and SPR-A joints after exposure to neutral salt spray environments for different aging times were tested. The results showed that the shear and fatigue properties of the joints gradually reduced with the increase of salt spray time. In addition, the SPR-A joints not only have higher maximum failure loads but also have larger energy absorption values in shear tests when compared with that of the SPR joints under the same salt spray time. The fatigue test results showed that fretting wear between the rivet-foot and the lower sheet is the main failure mechanism of joints. Moreover, the salt spray time has no obvious effect on the fatigue failure modes of the two kinds of joints.

Abstract Background:m7G methylation-associated genes were selected as the main research perspectives in this study, establishing a novel m7G methylation-associated miRNA risk signature for prognostic value in patient with carcinoma of colon. Methods:We identified two genes that are m7G methylation-associated and have been validated by searching in the PubMed database.The m7G methylation-associated miRNAs were screened via estimating the differentially expressed of miRNAs in 457 carcinoma of colon patient samples and 8 matched samples from a database named The Cancer Genome Atlas (TCGA). SelectingSingle-factor Cox analyses as well as multi-factor Cox analyses constructed the risk signature of 12 m7G methylation-associated miRNAs.Nomograms was adopted to predict the potential impact of m7G methylation-associated miRNAs on occurrence, development.The functions of DEGs that deserving to be noticed were classified and analyzed by functional enrichment analysis.Disease ontology analysis was used to explore the potential connection various diseases and carcinoma of colon.The outcome of associated analysis about immunocyte or immunological functioning revealed that what role does immunocyte or immunological functioning play in the development of carcinoma of colon.Finally, the relationship between high or low expression of significantly DEGs and immune cells and immune function was analyzed. Results:The results of nomogram analysis were as follows that the risk signature might predict the prognosis and the overall cumulative probability of survival（1-,3-,5-year） effectively about patients with carcinoma of colon. Through the analysis of Kaplan-Meier curve outcomes, we can draw a conclusion that depressedly expressed of hsa-miR-21-3p might be significantly relevant with decreased survival of patients.What the analyses of Gene ontology (GO) , Kyoto Encyclopedia of Genes and Genomes (KEGG) and Disease ontology（DO）,taking advantage of the common DEGs about risk score and immune score,demonstrated that these common DEGs play a partial role in the occurrence and development of tumors.Through disease ontology analysis,we found that eating disorder might be associated with carcinoma of colon. Conclusions: m7G methylation-associated miRNAs were related to the prognosis of patients with carcinoma of colon. A risk signature established based on these miRNAs could effectively predict the survival rate of later period in patients with carcinoma of colon.According to the displayed outcom from Our research that m7G methylation-associated miRNA regulators can serve as reliable prognostic biomarkers of carcinoma of colon, which might Greater probably be applyed as potential targets of therapeutic strategies.

A series of 26 novel derivatives have been synthesized through structural modification of gentiopicroside, a lead COX-2 inhibitor. And their in vivo and in vitro anti-inflammatory activities have been investigated. The in vitro anti-inflammatory activities were evaluated against NO, PGE2, and IL-6 production in the mouse macrophage cell line RAW264.7 stimulated by LPS. Results showed that most compounds had good inhibitory activity. The in vivo inhibitory activities were further tested against xylene-induced mouse ear swelling. Results demonstrated that several compounds were more active than the parent compound gentiopicroside. The inhibition rate of the most active compound P23 (57.26%) was higher than positive control drug celecoxib (46.05%) at dose 0.28 mmol·kg-1. Molecular docking suggested that these compounds might bind to COX-2 and iNOS. Some of them, e.g P7, P14, P16, P21, P23, and P24, had high docking scores in accordance with their potency of the anti-inflammatory activitiy, that downregulation of the inflammatory factors, NO, PGE2, and IL-6, was possibly associated with the suppression of iNOS and COX-2. Therefore, these gentiopicroside derivatives may represent a novel class of COX-2 and iNOS inhibitors.

In this study, low cycle reciprocating load tests were conducted on nine recycled aggregate concrete-filled steel tube columns (RACSTCs) and a solitary ordinary concrete-filled steel tube contrast column (OCSTC). The axial compression ratio and steel-tube wall thickness were considered as the parameters of interest. Accordingly, the failure mechanism, hysteresis performance, skeleton curve, ductility coefficient, energy consumption performance, and stiffness degradation of the two types of columns were compared. A vision measurement system was developed to track the full field deformations of specimens, and a quadocular vision system was constructed to determine the concrete column deformations. The deformations were compared with those obtained from laser measurements using an image and point cloud stitching algorithm. The results show that the hysteresis curves of RACSTCs are non-pinched with large ductility coefficients for all the specimens and the equivalent viscous damping coefficients are in the range of 0.462–0.975. RACSTC specimens exhibit good seismic and deformation performance. A three-fold restoring force model was established for RACSTC specimens based on skeleton curve test data. The theoretical hysteresis curve obtained using the skeleton curve model and hysteresis rule shows good agreement with the hysteresis curve obtained from experiment. This can be used as a reference for research on the restoring force characteristics of RACSTCs. The results obtained using the vision measurement system show that the measured mean relative error values are less than 0.504%. This confirms that the multiple vision measurement method can be used to accurately measure curved surface deformations as well as the full field strain values of RACSTCs. Therefore, the proposed method can be used to evaluate the asymmetric deformation of structures subjected to vibrations, impact, and other load types, thereby providing early warning decision data.

A facile Stevens rearrangement of the Weinreb amide and the subsequent key steps mediated by the carbonyl of the Weinreb amide led to the construction of azaspirocyclic skeletons of some typical alkaloids. And the formal total synthesis of (±)-cephalotaxine was completed via a shorter synthetic route by this efficient method. Further studies on the development and asymmetric synthesis application of this strategy are underway.