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Abstract WMP101: Global Burden of Stroke Attributable to High BMI in 38 OECD Countries From 1990-2019: A Benchmarking Systematic Analysis

Background: The global burden of stroke remains a significant public health challenge, with high body mass index (BMI) emerging as a notable and modifiable risk factor in Organization for Economic Co-operation and Development (OECD) countries. Method: Using Global burden of disease tool, deaths and disability adjusted life years due to Stroke attributable to High BMI were assessed by age, sex, year across the 38 OECD countries from 1990-2019. Result: The total number of deaths saw a decline from 148,031 (95%UI 85,206-219,328) in 1990 to 143,105 (95%UI: 85,383-209,533) in 2019. The annual percentage change (APC) in the total number of DALYs exhibited a decrease of 1% between 1990-2019. Notably, the most substantial increase in APC for deaths was observed in Turkey at 129%, followed by Mexico at 95% from 1990-2019. Conversely, Estonia displayed the most significant decrease in APC at 63%. In relation to DALYs, Turkey experienced the highest APC at 99%, followed by Costa Rica at 85% from 1990-2019. In 2019, the age group of 75-79 exhibited the highest number of deaths (22,709) while the age group of 65-69 had the highest DALYs (553,284). In terms of gender differences, males observed a 5% increase in APC for deaths, whereas females witnessed a 10% decrease in APC from 1990-2019. Conclusion: The global burden of stroke attributable to high BMI in OECD countries highlights the urgent need for comprehensive strategies to address the intertwined challenges of obesity and stroke. Efforts to prevent and manage obesity, coupled with targeted interventions to mitigate stroke risk factors, hold the promise of reducing the human, societal, and economic toll of this prevalent and debilitating condition.

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Abstract WP230: Global Burden of Stroke and Its Attributable Risk Factors in High Income North America From 1990-2019: A Systematic Analysis for the Global Burden of Disease Study 2019

Background: Stroke ranks as the 2 nd leading cause of mortality, following ischemic heart disease, within the spectrum of cardiovascular-related deaths. Method: Employing the Global Burden of Disease tool, Stroke prevalence, incidence, mortality, and Disability-Adjusted Life Years (DALYs) by age, sex, and year across the High-Income North America (HINA) countries from 1990-2019. Result: The overall prevalence of stroke increased from 4,796,172 (with a 95%UI:4,289,092-5,345,234) in 1990 to 7,703,036 (95% UI: 6,967,262-8,537,862) in 2019. The annual percentage of change (APC) demonstrated an increment of 22% in incidence, 21% in DALYs, and 23% in mortality from 1990-2019. Notably, the most substantial APC in mortality was observed in Mexico, at 74% from 1990-2019, whereas the lowest increase was seen in the United States at 23% during the same period. Notably, all countries exhibited a significant reduction in the burden of stroke from 1990-2019. Within the age groups, the highest number of deaths, 37,559, occurred among individuals aged 85-89, whereas the highest incidence, 64,422, was noted in the 70-74 age group. In terms of DALYs, the 75-79 age group accounted for 516,753 in 2019. Throughout the assessed period, males bore a greater burden than females. High blood pressure emerged as the leading attributable cause of stroke-related deaths, contributing to 89,009 fatalities in 2019. Conclusion: The burden of stroke within High-Income North America embodies intricate dimensions spanning health, economics, and society. This study underscores the imperative for targeted interventions that address the distinctive challenges posed by stroke within this specific region.

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Evaluating the effectiveness and outcomes associated with direct peritoneal resuscitation in damage control surgery patients with and without hemorrhagic shock

IntroductionThis narrative review aims to evaluate the efficacy of adjunct direct peritoneal resuscitation (DPR) in the treatment of adult damage control surgery (DCS) patients both with and without hemorrhagic shock, and its impact on associated outcomes. MethodsPubMed, Google Scholar, EMBASE, ProQuest, and Cochrane were searched for relevant articles published through April 13th, 2023. Studies assessing the utilization of DPR in adult DCS patients were included. Outcomes included time to abdominal closure, intra-abdominal complications, in-hospital mortality, and ICU length of stay (ICU LOS). ResultsFive studies evaluating 437 patients were included. In patients with hemorrhagic shock, DPR was associated with reduced time to abdominal closure (DPR 4.1 days, control 5.9 days, p = 0.002), intra-abdominal complications including abscess formation (DPR 27 %, control 47 %, p = 0.04), and ICU LOS (DPR 8 days, control 11 days, p = 0.004). Findings in patients without hemorrhagic shock were conflicting. Closure times were decreased in one study (DPR 5.9 days, control 7.7 days, p < 0.02) and increased in another study (DPR 3.5 days, control 2.5 days, p = 0.02), intra-abdominal complications were decreased in one study (DPR 27 %, control 47 %, p = 0.04) and similar in another, and ICU LOS was decreased in one study (DPR 17 days, control 24 days, p < 0.002) and increased in another (DPR 13 days, control 11.4 days, p = 0.807). ConclusionIn patients with hemorrhagic shock, adjunct DPR is associated with reduced time to abdominal closure, intra-abdominal complications such as abscesses, fistula, bleeding, anastomotic leak, and ICU LOS. Utilization of DPR in patients without hemorrhagic shock showed promising but inconsistent findings.

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Role of the Gut Bacteria-Derived Metabolite Phenylacetylglutamine in Health and Diseases.

Over the past few decades, it has been well established that gut microbiota-derived metabolites can disrupt gut function, thus resulting in an array of diseases. Notably, phenylacetylglutamine (PAGln), a bacterial derived metabolite, has recently gained attention due to its role in the initiation and progression of cardiovascular and cerebrovascular diseases. This meta-organismal metabolite PAGln is a byproduct of amino acid acetylation of its precursor phenylacetic acid (PAA) from a range of dietary sources like egg, meat, dairy products, etc. The microbiota-dependent metabolism of phenylalanine produces PAA, which is a crucial intermediate that is catalyzed by diverse microbial catalytic pathways. PAA conjugates with glutamine and glycine in the liver and kidney to predominantly form phenylacetylglutamine in humans and phenylacetylglycine in rodents. PAGln is associated with thrombosis as it enhances platelet activation mediated through the GPCRs receptors α2A, α2B, and β2 ADRs, thereby aggravating the pathological conditions. Clinical evidence suggests that elevated levels of PAGln are associated with pathology of cardiovascular, cerebrovascular, and neurological diseases. This Review further consolidates the microbial/biochemical synthesis of PAGln and discusses its role in the above pathophysiologies.

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Open Access
Assessing Freshwater Microbiomes from Different Storage Sources in the Caribbean Using DNA Metabarcoding.

Next-generation sequencing (NGS) and the technique of DNA metabarcoding have provided more efficient and comprehensive options for testing water quality compared to traditional methods. Recent studies have shown the efficacy of DNA metabarcoding in characterizing the bacterial microbiomes of varied sources of drinking water, including rivers, reservoirs, wells, tanks, and lakes. We asked whether DNA metabarcoding could be used to characterize the microbiome of different private sources of stored freshwater on the Caribbean Island nation of Antigua and Barbuda. Two replicate water samples were obtained from three different private residential sources in Antigua: a well, an above-ground tank, and a cistern. The bacterial microbiomes of different freshwater sources were assessed using 16S rRNA metabarcoding. We measured both alpha diversity (species diversity within a sample) and beta diversity (species diversity across samples) and conducted a taxonomic analysis. We also looked for the presence of potentially pathogenic species. Major differences were found in the microbiome composition and relative abundances depending on the water source. A lower alpha diversity was observed in the cistern sample compared to the others, and distinct differences in the microbiome composition and relative abundance were noted between the samples. Notably, pathogenic species, or genera known to harbor such species, were detected in all the samples. We conclude that DNA metabarcoding can provide an effective and comprehensive assessment of drinking water quality and has the potential to identify pathogenic species overlooked using traditional methods. This method also shows promise for tracing the source of disease outbreaks due to waterborne microorganisms. This is the first study from small island countries in the Caribbean where metabarcoding has been applied for assessing freshwater water quality.

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Open Access
Statewide Burden and Trends of Acute Myeloid Leukemia Attributable to Smoking in the United States of America from 1990-2019: A Benchmarking and Comparative Analysis

Background: Smoking has long been associated with an increased risk of various cancers, and it plays a significant role in deaths related to Acute Myeloid Leukemia (AML), accounting for 1.69% of all cancer-related deaths in the United States of America (USA). Additionally, among all other risk factors contributing to AML development, smoking is responsible for a staggering 77.2% of AML-related deaths. However, there is a large statewide variation within the USA concerning AML burden. Comparable and consistent state-level measures of total AML burden have not been produced previously. Method: Using the Global Burden of Disease methodology “AML attributable to smoking” mortality, and disability-adjusted life years (DALYs) were analyzed by age group, sex and year from 1990-2019 for all residents in the USA using standardized approaches for data processing and statistical modeling. Results were produced in total number and age-standardized rate. The findings were reported both in absolute numbers and age- standardized rates. Results: The total number of deaths attributed to AML due to smoking showed an increase from 2,230 (95% uncertainty interval (UI): 1,159-3,275) in 1990 to 3,918 (95% UI: 1,833- 6,003) in 2019. Similarly, the DALYs (Disability-Adjusted Life Years) associated with AML increased from 52,843 (95% UI: 30,918-75,041) in 1990 to 81,356 (95% UI: 43,328-121,964) in 2019. However, the age-standardized mortality rate (ASMR) remained relatively stable, with no major difference observed (0.7 deaths per 100,000 95% UI: 0.38-1.02) in 1990 compared to 0.7 (95% UI: 0.35-1.05) in 2019. In contrast the age-standardized DALYs rate (ASDALR) decreased from 17.51 to 15.3 per 100,000 from 1990 to 2019. Regarding the highest annual percentage of change (APC) in total number of deaths due to AML, Nevada observed the greatest increase at 275% followed by Alaska at 236% and Arizona at 179% from 1990 to 2019. In contrast, the District of Columbia was the only state that showed a decrease in APC for total deaths with a decline of 6%. Analyzing ASMR, Indiana, and West Virginia observed the highest APC at 28% each, followed by Kentucky at 23%. Conversely, California experienced the highest decrease in ASMR APC, showing a decline of 30% from 1990 to 2019. Regarding age groups, the 95+ age group observed the highest APC increase in ASMR and ASDALR at 363% and 356%, respectively, followed by the 90-94 age group, which had an ASMR APC increase of 307% and ASDALR APC increase of 305%. Moreover, males exhibited a higher APC increase in the total number of deaths compared to females, with percentages of 85% and 62%, respectively, from 1990 to 2019. Conclusion: Large disparities in the total burden of AML attributable to smoking persist between US states despite marked improvements in management. Our findings show that a substantial proportion of AML cases in the United States are attributed to smoking, making it a modifiable risk factor for this aggressive hematologic malignancy. Healthcare professionals and policymakers should utilize this evidence to reinforce anti-smoking initiatives and raise public awareness about the link between smoking and AML.

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Open Access
2595. Burden of Lower Respiratory Tract Infection in United States of America and its Trend from 1990-2019: A Benchmarking Analysis from the Global Burden of Disease Study

Abstract Background Lower respiratory infections (LRIs) cause substantial mortality and morbidity in the United states of America (USA) accounting for 2.78% of all causes of deaths. However, there is a large statewide variation within the United States with regard to LRIs burden. Comparable and consistent state-level measures of total LRIs burden have not been produced previously. Methods We used Global Burden of Disease Methodology to estimate the burden of LRIs by Age-groups, year, sex, location across the USA. Results In 2019, there were 299,564(95% UI 281,732–317,390) prevalent cases of LRIs in the USA, with an age-standardized rate (ASR) of 85.2 (95% UI 78.8-91.9) per 100,000 population. The annual percentage change of total number incidence increased by 14% followed by deaths increased 13% from 1990-2019. Furthermore, Mississippi [20.49 (95% UI 17.4–23.9)] per 100,000 and Tennessee [19.1 (95% UI 16–22.5)]per 100,000 had the highest age-standardized mortality rate (ASMR) in 2019. In 2019, the incidence and DALY were highest in the 60-64 age group,75-79 age group respectively in both females and males. Age-Standardized Incidence, Mortality and DALYs Lower Respiratory Tract Infection in United States, 2019 Trend of Lower Respiratory Tract Infection in USA from 1990-2019 A) All age-counts, Incidence, Death, DALY B) Age-standardized rate Incidence, Death, DALY Sex-wise distribution of Lower Respiratory Tract Infection, Deaths, per 100,000 across the United States Conclusion LRIs continue to be a significant burden on the healthcare system in the United States, causing a significant economic impact and affecting vulnerable populations disproportionately. Preventive measures and prompt treatment can help reduce this burden and improve the overall health of the population. Disclosures All Authors: No reported disclosures

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Open Access
2588. Burden of Mortality and Antimicrobial Resistance of Bacterial Pathogens Associated with Lower Respiratory Tract and Other Thorax Infection in United States in 2019: A Systematic Analysis

Abstract Background Detailed information about the impact of bacterial antimicrobial resistance (AMR) on Lower Respiratory Tract and other thorax Infections (LRTIs) is currently unavailable. It is important to have precise data on bacterial AMR to develop effective programs and policies for controlling AMR and to use antibiotics prudently for optimal treatment of LRTI patients. The primary objective of this research is to present extensive estimates of the mortality due to "bacterial pathogens" and its "AMR" of LRTIs in the United States in 2019. Methods Mortality data associated with bacterial antimicrobial resistance and lower respiratory tract infection (LRTI) pathogens were acquired from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) in 2019. Results In United States, there were 88089 deaths (95% uncertainty interval [UI]: 66909-117145) due to bacterial pathogens in LRIT in 2019. Highest number of deaths observed due to Staphylococcus aureus (34.04%), followed by Streptococcus pneumoniae (18.30%), Pseudomonas aeruginosa (12.48%) in LRTI in 2019. There were 10984 deaths attributed to and 46482 deaths associated with bacterial AMR in LRTI in 2019. Conclusion Bacterial pathogens causing LRTIs are a significant cause of mortality in the United States. Moreover, antimicrobial resistance (AMR) among these pathogens has become a growing concern, exacerbating the burden of LRTI-related deaths. The implementation of such measures, along with the promotion of antimicrobial stewardship, can help to mitigate the impact of LRTI-associated deaths and preserve the effectiveness of available antimicrobial agents for the future. Disclosures All Authors: No reported disclosures

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Open Access