Exploitation of natural resources of the marine ecosystem to feed a growing human population has led to the possibility of their application in the future. In order to solve this problem, mariculture develops and the create adequate conditions for the breeding of marine organisms which are using in human nutrition and one of them are shellfish known as nutritionally valuable food. Microflora of marine organisms, and therefore shells largely depends on environmental factors such as time of the year, temperature of the sea water etc. This work was focused on growing shells on the Montenegrin coast, and a special emphasis was put on placing health-safe shells on the Montenegrin food market. In addition to meat shells, physical and chemical parameters of sea water were studied: water temperature, salinity, oxygen saturation, pH, transparency, nitrate concentration, Examination of these parameters is related to the period of one year. The own results of the microbiological examination of the samples of shellfish sampled in six production zones in April according to presence of Escherichia coli in the value of ˂18 MPN – (Most Probably Number) per 100 g of meat and intra-shell liquid, which means that shellfish in general at the Montenegrin coasts satisfy and meet microbiological criteria in view of the presence of Escherichia coli. When its own results compare with the results of the SVL (Specialist Veterinary Laboratory) for the same time in each zone, it can be determined that the results match, thus confirming the accuracy of the test results shown.

Natural Protected Areas play a fundamental role in the conservation of biodiversity and ecosystems throughout the world and are designated and managed with the purpose of preserving biological and cultural capital, ensuring the sustainable use of natural resources, and maintaining ecosystem services. The Montes Azules Biosphere Reserve, like other Natural Protected in Mexico, faces significant challenges such as deforestation, illegal logging, and loss of vegetation cover, as well as socio-environmental conflicts, factors that hinder the biological and cultural conservation of the area. Likewise, despite their importance, many Natural Protected face significant challenges in terms of effective monitoring and planning. The lack of adequate monitoring and planning strategies, as well as staff and budget, makes it difficult to assess the conservation status of these areas and to implement appropriate management measures. Therefore, creating specific monitoring tools is essential to periodically evaluate their conservation status and identify threats and pressures on ecosystems in order to design adaptive and effective management strategies. The objective of this study is to offer a first methodological approach to identify priority areas for restoration within the Montes Azules Biosphere Reserve, which will allow decision-makers to design and apply conservation strategies in the area. The determination of the priority areas for restoration was based on an environmental management process, where the Land suitability is the base for the territorial arrangement, which was modeled together with the current land use and land cover through rules of decision and having the natural protected area as a frame. For land suitability, the land classification system was used according to its use capacity or American system of the 8 classes developed by the Soil Conservation Service of the United States; for mapping land use land cover, an object base classification method was used. The total transformed area in 2023 occupies an extension of 39,475.38 ha, approximately 50% of this lands corresponds to sloping and very steep land and in some cases with shallow to very shallow soils, not suitable for agriculture. The area identified as priority zones for restoration corresponds to an extension of 19,514.5 ha, which represents nearly 50% of the transformed area, and 6% of the total of study area, from which 6,860.5 ha are top priority for restoration. As main conclusion can be said that the methodological proposal described here serves as a first approximation to identify priority zones for restoration in the Montes Azules Biosphere Reserve, allowing for a more detailed identification of these areas at the local level, which will allow decision-makers to design and apply conservation strategies in the area.

This study investigates the degradation of the azo dye (Disperse Red) using a rhizosphere bacterial consortium. Standard microbiological and molecular techniques were employed to isolate and identify organisms from rhizosphere soil. Degradation of azodye was carried out in a fabricated anoxic and oxic chambers with hydraulic retention time of 40hrs. Initial identification of the bacterial isolates through Gram’s reaction and biochemical tests revealed the presence of organisms belonging to the genera Pseudomonas, Lysinibacillus, and Citrobacter. Molecular and phylogenetic analyses confirmed the isolates as Pseudomonas aeruginosa, Lysinibacillus sphaericus, Pseudomonas chengduensis, and Citrobacter freundii. During the preliminary testing, the degradation efficiency was assessed under varying glucose concentrations. Higher decolorization rate of 56.17% was observed in the medium with 10% glucose after 72 hours, while the medium with 5% glucose achieved a 44.17% colour reduction. Notably, lower degradation rates recorded were 11.96% and 12.85% for the 5% and 10% dye enhance glucose mineral salt media, respectively. However, During the actual degradation testing in a double-chamber system enhanced with biochar, the first anaerobic cycle achieved a maximum decolorization of 71.95% after 94 hours, with the first aerobic cycle further enhancing degradation to 90.51%. The second anaerobic cycle increased degradation to 94.78%, and the final aerobic cycle achieved a decolorization of 98.47%. These results show that the rate of Disperse Red degradation is highly dependent on glucose levels and alternating anaerobic-aerobic conditions. This study demonstrates the potential of using rhizosphere bacterial consortia to bioremediate wastewater contaminated with azo dyes, offering an efficient and sustainable method of environmental management. The results underline the need of optimizing ambient conditions to increase microbial degradation processes.

This study investigated the removal of nitrogen and phosphates in fish processing wastewater through process optimization of a membrane bioreactor (MBR) treatment unit. Raw process wastewater was collected from the Makindi fish farm and was pre-filtered through a mesh of 0.8 mm. The experiment was conducted at a lab scale using commercial Polyethersulfone (PES) membranes submerged in the MBR unit. The pre-filtered wastewater sample was added into a denitrification (anoxic) tank. The process was conducted through continuous recirculation between the aeration and the anoxic tank to enhance the removal of nitrogenous compounds. The recirculation flow rate was 10L/h. A hydraulic residence time (HRT) of approximately 22-25h was maintained, and the aeration rate in the aerobic tank was 100L/min. Aluminum sulphate AI2(SO4)318H2O was added continually into the anoxic tank and with constant stirring to enhance the removal of phosphates. A removal rate of 85±2% for NH4+-N, 84±1% for NO3- -N, and 69±3% for PO43--P was obtained. The nutrient concentration in the effluent was successfully reduced to an acceptable level with both nitrogenous compounds and phosphate concentrations obtained within the range of < 30 mg/L and ≤ 5 mg/L as per the WHO guidelines for wastewater reuse for irrigation. The results showed a successful process optimization that can be applied to ensure optimized performance of the MBR unit thus improve its effectiveness for the treatment of fish processing wastewater. The study therefore recommends process optimization as a tool for effective removal of nutrients in the MBR system thus making it a potential recycling approach for treatment of fish processing wastewater for reuse for irrigation purposes.

The issue of street trees in urban planning is being addressed in Brazzaville, Congo. The aim is to improve urban space, in particular resilience for the well-being of city dwellers, by making the most of trees. As little attention has been paid to spatiotemporal research into the place of avenue trees in urban planning, the study targets ecosystem services rendered to society, once the paving of communication arteries is effective. The methodology is based on the inventory and enumeration of arborescent woody plants d1.30 ≥ 10 cm, present in 32 arteries, meeting precise criteria. Notwithstanding the measurement of d1.30 and tree height, the data collected includes the distance between trees and roadways, the area reserved for the plant and an assessment of the damage caused. The inventory lists 1,866 woody plants grouped into 41 species, 30 genera and 15 families. The allochthonous-dominated flora cohort includes several senescent individuals. Urban resilience and well-being are underpinned by above-ground biomass (81.68 t/ha/year) and sequestered carbon (40.84 t/ha/year). The carbon dioxide (CO2) sequestered would be 386.69 tones. In terms of infrastructure destruction, Terminalia mantaly and Terminalia catappa come out on top.

In order to describe the negative aspects produced by the industrialized production of food that we present in this article, I have coined a definition that summarizes all the negative contents of the current diet: "PESTIFOOD", which is the portmanteau of the words PESTICIDE and FOOD. After a brief premise on the importance that healthy food has for the survival of living things, I critically describe the intensive production of food and the negative impact it has had on the emergence of numerous metabolic pathologies. So that due to the lack of nutritional value of the foods that industrial production provides us, we must record the growing development of new pathologies linked mainly to junk food and Pestifood. In the current state of degradation of food resources, not even the adoption of fortified foods or supplements can solve the root problem, represented by the use of chemical substances to improve production and extend the expiry date of foods. The only solution to the food problem for the future will be that of eco-sustainable choices, to recover the value of food as "our true medicine" - Hippocrates.

Everything scientists can observe in the universe, from people to planets, is made of matter. Matter is defined as any substance that has mass and occupies space. But there’s more to the universe than the matter we can see. Dark matter and dark energy are mysterious substances that affect and shape the cosmos, and scientists are still trying to figure them out. Invisible dark matter makes up most of the universe (96 %) – but we can only detect it from its gravitational effects. Galaxies in our universe seem to be achieving an impossible feat. They are rotating with such speed that the gravity generated by their observable matter could not possibly hold them together; they should have torn themselves apart long ago. The same is true of galaxies in clusters, which leads scientists to believe that something we cannot see is at work. They think something we have yet to detect directly is giving these galaxies extra mass, generating the extra gravity they need to stay intact. This strange and unknown matter was called “dark matter” since it is not visible. The melanin of living beings and the dark matter of the universe share the same physical/chemical characteristics, including the unsuspected ability to transform the power of light into chemical energy, by dissociating water molecules, as in plants.

Effective, selective, precise, and accurate liquid chromatographic analytical methods for the determination of difenoconazole fungicide in the formulation have been optimized and validated to meet the accreditation requirements, the performance characteristics of the analytical method were validated which is also one of the basic requirements of ISO Standard 17025:2017, for the rapid determination of difenoconazole by HPLC-DAD. The used method involves the extraction of the substances by sonication of the sample with acetonitrile, followed by dilution in acetonitrile, and direct injection on a liquid chromatography system. For the analysis LC system from Agilent Technologies 1290 Infinity II was used. Good separation was achieved on a Luna C18 column (3µm, 100°A, 3x150mm) with a guard column (C18 4x2.0mm I.D), an isocratic mobile elution consisting of acetonitrile and water acidified 0.075% with formic acid (85:15, v/v), at a flow rate of 0.7 ml/minute and UV detection at 220 nm. The column temperature was 25⁰C, injected volume was 1 μL. The analysis duration was 10 minutes (the retention time of difenoconazole and 4 – Hydroxybenzoic acid – methyl ester was 1.846 and 1.135 minutes, respectively. The linearity within the concentration range of 750-125 µg mL-1, with the internal standard at a concentration of 250 µg mL-1 with an average correlation coefficient (R2) of 0.9998. We have considered precision, repeatability, and selectivity in the validation.