Zataria Research Articles

Therapeutic effects of medicinal plants in vulvovaginal candidiasis: a review on randomized clinical trials

Introduction: Vulvovaginal candidiasis (VVC), characterized by cheesy vaginal discharge, pain during sexual intercourse and itching is the fungal infection of female genital tract. Candida albicans is the common causative agent for this condition followed by many non-albicans Candida species. Due to the greater concern of drug resistance in Candida species, many alternative therapeutic methods have been proposed, including the use of various plant extracts. The alternate methods are effective in treating the symptomatic cases by reducing the clinical symptoms and Candida load. The objective of this review is to explore the therapeutic effectiveness of various plant extracts in vulvovaginal candidiasis. Materials and methods: A literature survey was performed in PubMed, Web of Science, Scopus and Google Scholar to find relevant clinical trials on vulvovaginal candidiasis. After the removal of duplicates, a total of 21 clinical trials were considered to evaluate the anti-Candida activity of medicinal plant products against vulvovaginal candidiasis. Results: The results of the clinical trials show that, the extracts from Curcuma longa, Zingiber officinale, Allium sativum, Zataria multiflora and Lawsonia inermis have showed potent anti-Candida activity and many other plant products were found to be as effective as the conventional drugs used. Conclusion: Due to the limited number of clinical trials available, it is difficult to come to a conclusion regarding the clinical effectiveness of various plant extracts in the effective treatment of vulvovaginal candidiasis. So, more research is needed to evaluate the effectiveness of each of these plant extracts as therapeutic alternatives to treat this condition.

Effects of Lamiaceae family plants and their bioactive ingredients on coronavirus-induced lung inflammation.

Coronaviruses (CoVs) are a family of viruses that cause infection in respiratory and intestinal systems. Different types of CoVs, those responsible for the SARS-CoV and the new global pandemic of coronavirus disease 2019 in people, have been found. Some plants were used as food additives: spices and dietary and/or medicinal purposes in folk medicine. We aimed to provide evidence about possible effects of two Lamiaceae family plants on control or treatment of CoVs-induced inflammation. The keywords including coronaviruses, Thymus vulgaris, Zataria multiflora, thymol, carvacrol, antivirus, and anti-inflammatory and antioxidant effects were searched in various databases such as PubMed, Web of Sciences (ISI), and Google Scholar until September 2022. The medicinal herbs and their main ingredients, thymol and carvacrol, showed antiviral properties and reduced inflammatory mediators, including IL-1β; IL-6, and TNF-α, at both gene and protein levels but increased the levels of IFN-γ in the serum as anti-inflammatory cytokine. These medicinal herbs and their constituents also reduce oxidative stress and enhance antioxidant capacity. The results of molecular docking analyses also indicated that polyphenol components such as thymol, carvone, and carvacrol could inhibit the entry of the viruses into the host cells in molecular docking analyses. The antiviral, anti-inflammatory, and antioxidant effects of these plants may be due to actions of their phenolic compounds that modulate immune response and may be useful in the control and treatment of CoV-induced lung disorder.

The effect of essential oil of Zataria multiflora incorporated chitosan (free form and Pickering emulsion) on microbial, chemical and sensory characteristics in salmon (Salmo trutta)

The objective of current research was to prepare a new biodegradable coating containing chitosan (Ch) and zataria multiflora essential oil (ZMEO) (free and Pickering emulsion (PEO) forms), in order to enhance the Salmo trutta shelf life. Our results showed, the mean of films thickness, mechanical properties (elastic modulus (EM) and tensile strength (TS) analysis) and WVP in different treatments were ranged from 0.103 ± 0.003 (for Ch) to 0.109 ± 0.003 (for Ch-PEO (2.5 %)) µm for thickness, from 3.2 ± 1.6 (for Ch) to 8.15 ± 2.3 (for Ch-EO) MPa for EM, from 1.3 ± 0.5 (for Ch-EO) to 1.6 ± 0.06 (for Ch) Mpa for TS and from 0.1 ± 0.02 (for Ch) to 0.8 ± 0.05 (for Ch-EO) (×10 − 11(g m/m2 s Pa) for WVP. In current research, the lowest and highest total viable counts (TVC) was related to Ch-PEO (1.7 log CFU/g) and control treatments (4.65 log CFU/g). The lowest and highest of pH was related to the Ch-PEO (6.45) and the control (7.1), the lowest and highest of PV (peroxide value) was related to Ch-PEO (0.34 meq/kg) and control treatment (1.37 meq/kg), the lowest and highest of TBARS (thiobarbituric acid reactive substances) was related to Ch-PEO (0.37 mg/kg) and control treatment (2.23 mg/kg) and also the lowest and highest of TVB-N (total volatile base nitrogen) was related to Ch-PEO (17.7 mg) and control (59 mg). Also, Ch-PEO showed the best sensory properties after sixteen days. Among all the treatments in all the tests, the best maintenance property was related to the Ch-PEO, therefore, chitosan coatings containing ZM Pickering emulsion should be considered as a potential active coating in the fish industry.

Encapsulation of Zataria multiflora essential oil in electrosprayed zein microcapsules: Characterization and antimicrobial properties

This research was carried out to make zein microcapsules containing Zataria multiflora essential oil (Z. multiflora EO) by the electrospraying method to evaluate the antimicrobial properties of the microcapsules on Z. tritici and F. graminearum fungi, as well as X. translucens and R. tritici bacteria. To achieve this aim, Z. multiflora EO was encapsulated at different concentrations (0, 5,10%, and 15% v/v) in electrosprayed zein microcapsules and the characteristics of the electrosprayed zein microcapsules were examined using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier transform infrared (FTIR). Porosity and specific surface values were measured by the BET method. Then, the amount of loaded Z. multiflora EO was determined for efficiency. The antifungal and antibacterial activities of the electrosprayed zein microcapsules were measured using the agar well diffusion method. The results of SEM showed that the morphology of the electrosprayed zein microcapsule was influenced by the concentration of Z. multiflora EO (p < 0.05). DSC results indicated the compatibility between the Z. multiflora EO and the zein polymer. The FTIR spectrum showed that adding Z. multiflora EO changed the secondary structure of the zein protein. XRD patterns showed that the electrosprayed zein microcapsules were in a semi-crystalline state. The BET results determined the average pore diameter of microcapsules. Electrosprayed zein microcapsules containing Z. multiflora EO had inhibitory effects on both fungi and bacteria (p < 0.05). According to the results, the electrosprayed zein microcapsules containing Z. multiflora EO can be used as an antimicrobial compound for fruit and vegetable products.

Therapeutic potential of Zataria multiflora: A narrative review of current evidence

Zataria multiflora Boiss is a perennial plant with a wide spectrum of biological and pharmacological activities including antidiabetic, anti-nociceptive, anti-asthmatic, anti-fever, anti-spastic, anti-oxidative, anti-inflammatory, and antimicrobial properties. This paper reviews the therapeutic effects of Zataria multiflora based on recent reports. The relevant reports were extracted by checking the electronic databases including PubMed, Scopus, Web of Science, and Google Scholar from the beginning of 2010 until the end of May 2023. The neuroprotective effects of Zataria multiflora can be attributed to inhibition of acetylcholinesterase, enhancement of brain-derived neurotrophic factor, and alleviation of brain oxidative damage. Zataria multiflora also exerts its protective effects on the respiratory system, liver, and kidney by reducing the level of inflammatory cytokines, scavenging the free radicals, and augmenting the antioxidant enzymes. Additionally, Zataria multiflora accelerates wound healing via upregulating transforming growth factor-β, insulin-like growth factor 1, fibroblast growth factor 2, and vascular endothelial growth factor, and inducing angiogenesis and collagen biosynthesis. Overall, the protective impacts of Zataria multiflora on different organs are mainly attributed to its antioxidant and anti-inflammatory properties.

Inhaled paraquat-induced lung injury in rat, improved by the extract of Zataria multiflora boiss and PPARγ agonist, pioglitazone

The effects of a PPAR-γ agonist, pioglitazone and Zataria multiflora (Z. multiflora) on inhaled paraquat (PQ)-induced lung oxidative stress, inflammation, pathological changes and tracheal responsiveness were examined.The study was carried out in control rats exposed to normal aerosol of saline, PQl and PQh groups exposed to aerosols of 27 and 54 mg/m3 PQ, groups exposed to high PQ concentration (PQh) and treated with 200 and 800 mg/kg/day Z. multiflora, 5 and 10 mg/kg/day pioglitazone, low doses of Z. multiflora + pioglitazone, and 0.03 mg/kg/day dexamethasone.Increased tracheal responsiveness, transforming growth factor beta (TGF-ß) and lung pathological changes due to PQh were significantly improved by high doses of Z. multiflora and pioglitazone, dexamethasone and extract + pioglitazone, (p < 0.05 to p < 0.001). In group treated with low doses of the extract + pioglitazone, the improvements of most measured variables were significantly higher than the low dose of two agents alone (p < 0.05 to p < 0.001).Z. multiflora improved lung injury induced by inhaled PQ similar to dexamethasone and pioglitazone which could be mediated by PPAR-γ receptor.

Active packaging from natural ingredients applied to meat: A review

Meat is a highly perishable food that requires proper preparation and treatment in order to extend its longevity. Active packaging is an innovation that continues to be developed for the food industry. Active packaging is a modern technology that aims to extend the shelf life, and maintain or improve the condition of packaged food for the better, by incorporating active ingredients into the packaging that are capable of releasing or absorbing substances from or into the packaged food or the environment. The use of chemicals in meat preservation and packaging gives a sense of worry to consumers. Researchers are looking for alternatives to chemicals, namely from natural ingredients used in the active packaging of meat. The use of natural herbal ingredients combined with various preservatives aims to increase efficiency and minimize the effects on the organoleptic quality of meat. Testing various extracts of natural ingredients into active packaging applied to meat showed good results. The results showed that various natural extracts contain compounds that have antimicrobial, antioxidant, and lipid-activating properties. Natural ingredients such as citrus extracts, propolis extracts, and essential oils of zataria multiflora (zeo), mustard seeds, the green, rambutan, garlic, and Origanum vulgare flowers have antioxidant compounds, and antimicrobial compounds and are able to reduce lipid oxidation activity of meat, and the results of research using mustard seed extracts, the green, garlic, Origanum vulgare flowers able to increase the shelf life of meat.

Comparison of the Content of Phenolic Compounds, Flavonoids, and Antioxidant Activity of Six Different Medicinal Plants under Extraction Conditions with Different Solvents

Background: In the present study, the solvent effect (aqueous and hydroalcoholic) of the extraction on the content of phenol, flavonoid, and the antioxidant activity of six medicinal plants, including Persian oak fruit hull (Quercus brantii), Zataria multiflora, German Matricaria chamomilla, Teucrium polium, Rosa foetida Herrm, and mountain barberry (Berberis integerrima) was investigated. Materials and Methods: To measure phenol and flavonoid content, folin ciocaltio and aluminum chloride reagents were used, respectively, and to measure the antioxidant potential, the DPPH method was used. The content of phenol and flavonoid compounds was calculated as mg equivalent of gallic acid and catechin per gram of dry weight of the plant, respectively. The collected data was analyzed in SPSS software (version 14). Results: According to the obtained results, the amount of phenol and flavonoid in the hydroalcoholic extract of oak fruit hull Quercus brantii, Zataria multiflora, German Matricaria chamomilla, and mountain barberry was more than the aqueous extract, and this difference was statistically significant (P&lt;0.001). The highest content of phenol and flavonoid were related to the hydroalcoholic extract of oak by 310.2 ± 2.5 mg/g and 241.2 ± 1 mg/g, respectively, and the lowest amounts were related to the aqueous extract of Matricaria chamomilla (53.1±1.1 mg/g) and the aqueous extract of acorn (23.9±1.5 mg/g), respectively. The highest antioxidant activity (IC50) was related to oak fruit-hull hydroalcoholic extract (39.1±4.4 µg/ml), and the lowest was related to the aqueous extract of Matricaria chamomilla (315.3±3.3 µg/ml). Conclusion: The findings of this study showed that the solvent used to extract plants is a significant factor in separation of phenol and flavonoid compounds as well as their antioxidant activity.

Encapsulated Zataria multiflora's essential oil inhibited the growth of Aspergillus flavus and reduced aflatoxins levels in contaminated pistachio nut

The essential oil of Zataria multiflora Boiss. (ZEO) was prepared using a British Pharmacopeia-type hydrodistillation apparatus. The ZEO was encapsulated with a natural polymer, chitosan (CS) by oil-in-water emulsification, and ionic cross-linking techniques. The shape and size range of the encapsulated particles were evaluated by Atomic-Force Microscopy (AFM) and Dynamic Light Scattering (DLS) and FT-IR spectroscopy. The AFM analysis of the ZEO encapsulated nano-particles (CS-ZEO) showed spherical shape particles with inner cavities. The mean size of CS-ZEO particles was 293 ± 17 nm according to DLS analysis. The chemical constituent of the oil was analyzed by GC-MS and thymol acetate (32.4%), carvacrol acetate (19.0%), γ-terpinene (18.5%), and ρ-cymene (14.5%) were reported as its major constituents. The non-encapsulated ZEO in the synthesis process was determined to calculate the encapsulation efficiency and loading capacity of the prepared CS-ZEO which were 87.4% and 6%; respectively. In vitro antifungal activity of ZEO and CS-ZEO in growth media against Aspergillus flavus was evaluated with the decrease in fungal mycelial mass. On the other hand, the HPLC results showed the reduction of aflatoxins B1 levels by 95% and 92% at concentrations of 0.8 mg/mL of ZEO and 6.7 mg/mL of CS-ZEO, respectively. The above-mentioned effective concentrations of the ZEO and CS-ZEO, were applied to pistachio samples, which reduced 90.5% and 99.0%, of the aflatoxin B1 levels, respectively compared to non-treated samples. Therefore, we suggest here, the CS-ZEO as a possible natural fungicide with an effective mechanism of lowering aflatoxins production in the contaminated pistachio.

Combined antifungal effects of the vapor phases of Zataria multiflora and Cinnamomum zeylanicum essential oils against Aspergillus flavus and Penicillium citrinum in vitro and cheese.

The objective of this study was to evaluate the antifungal effects of Zataria multiflora (ZEO) and Cinnamomum zeylanicum (CEO) essential oils in the vapor phase against Aspergillus flavus and Penicillium citrinum in vitro and cheese. The antifungal activities of the vapors of ZEO and CEO were assessed by determining the minimum inhibitory concentration (MIC), fractional inhibitory concentration (FIC) index, and inhibition zone diameters. Thymol (51.10%) and cinnamaldehyde (77.82%) were the main constituents of ZEO and CEO, respectively. The MIC values of the vapors of ZEO and CEO against A. flavus were 25 and 12.5 μL/L and against P. citrinum were 800 and 400 μL/L, respectively. The in vitro results showed that the combination of the vapor phases of ZEO and CEO could synergistically inhibit the growth of A. flavus (FIC index = 0.75). In the cheese, the growth of P. citrinum was entirely inhibited by the combination of ZEO and CEO vapors, even at very low concentrations (1/16 MIC). In conclusion, the vapor phases of ZEO and CEO showed the potential to be applied as effective natural antifungals and alternatives to synthetic preservatives in cheese.

Fabrication and characterization of antimicrobial hybrid electrospun polyvinylpyrrolidone/kafirin nanofibers activated by zataria multiflora essential oil

Fabrication and characterization of antimicrobial hybrid electrospun polyvinylpyrrolidone/kafirin nanofibers activated by zataria multiflora essential oil

Evaluation of the Bioactivity of Honey in Combination with Alcoholic Extract of Black Cardamom and Zataria multiflora

Considering the high rates of global antimicrobial resistance, researchers are looking for new ways to deal with the resistance. Therefore, this study is aimed at analyzing the bioactive compounds and antimicrobial effects of honey and alcoholic extracts of Zataria multiflora and Black cardamom. The mixture of honey (30%) and alcoholic extracts of Zataria multiflora (35%) and Black cardamom (35%) as named F6 revealed the antibacterial activity against three tested bacteria ( MIC = 1000 μg/ml). Thymol, resorcinol, and phenol, 2-methyl-5-(1-methylethyl, 3,7-octadiene-2, 6-diol, 2, 6-dimethyl) were identified as potential antimicrobial agents using GC-MS. The F6 toxicity to RBCs and HEK293 human cells was 11.13 and 13.84 times lower for Triton X-100 toxicity (95%), respectively. We concluded that F6 has the best antibacterial activity against E. coli and less toxicity against tested eukaryotic cells due to the presence of chemicals such as hydrogen peroxide, thymol, and resorcinol.

Zataria multiflora and its constituent, carvacrol, counteract sepsis-induced aortic and cardiac toxicity in rat: Involvement of nitric oxide and oxidative stress.

Zataria multiflora and carvacrol showed various pharmacological properties including anti-inflammatory and anti-oxidant effects. However, up to now no studies have explored its potential benefits in ameliorating sepsis-induced aortic and cardiac injury. Thus, this study aimed to investigate the effects of Z. multiflora and carvacrol on nitric oxide (NO) and oxidative stress indicators in lipopolysaccharide (LPS)-induced aortic and cardiac injury. Adult male Wistar rats were assigned to: Control, lipopolysaccharide (LPS) (1 mg/kg, intraperitoneal (i.p.)), and Z. multiflora hydro-ethanolic extract (ZME, 50-200 mg/kg, oral)- and carvacrol (25-100 mg/kg, oral)-treated groups. LPS was injected daily for 14 days. Treatment with ZME and carvacrol started 3 days before LPS administration and treatment continued during LPS administration. At the end of the study, the levels of malondialdehyde (MDA), NO, thiols, and antioxidant enzymes were evaluated. Our findings showed a significant reduction in the levels of superoxide dismutase (SOD), catalase (CAT), and thiols in the LPS group, which were restored by ZME and carvacrol. Furthermore, ZME and carvacrol decreased MDA and NO in cardiac and aortic tissues of LPS-injected rats. The results suggest protective effects of ZME and carvacrol on LPS-induced cardiovascular injury via improved redox hemostasis and attenuated NO production. However, additional studies are needed to elucidate the effects of ZME and its constituents on inflammatory responses mediated by LPS.

Encapsulation of Zataria multiflora essential oil in polyvinyl alcohol/chitosan/gelatin thermo-responsive hydrogel: Synthesis, physico-chemical properties, and biological investigations

Zataria multiflora essential oil is a natural volatile plant product whose therapeutic applications require a delivery platform. Biomaterial-based hydrogels have been extensively used in biomedical applications, and they are promising platforms to encapsulate essential oils. Among different hydrogels, intelligent hydrogels have recently attracted many interests because of their response to environmental stimuli such as temperature. Herein, Zataria multiflora essential oil is encapsulated in a polyvinyl alcohol/chitosan/gelatin hydrogel as a positive thermo-responsive and antifungal platform. According to the optical microscopic image, the encapsulated spherical essential oil droplets reveal a mean size of 1.10 ± 0.64 μm, which are in consistent with the SEM imaging results. Encapsulation efficacy and loading capacity are 98.66 % and 12.98 %, respectively. These results confirm the successful efficient encapsulation of the Zataria multiflora essential oil within the hydrogel. The chemical compositions of the Zataria multiflora essential oil and the fabricated hydrogel are analyzed by gas chromatography-mass spectroscopy (GC–MS) and Fourier transform infrared (FTIR) techniques. It is found that thymol (44.30 %) and γ-terpinene (22.62 %) are the main constituents of the Zataria multiflora essential oil. The produced hydrogel inhibits the metabolic activity of Candida albicans biofilms (∼60–80 %), which can be related to the antifungal activity of the essential oil constituents and chitosan. Based on the rheological results, the produced thermo-responsive hydrogel shows a gel-sol viscoelastic transition at a temperature of 24.5 °C. This transition leads to a facile release of the loaded essential oil. The release test depicts that about 30 % of Zataria multiflora essential oil is released during the first 16 min. In addition, 2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay demonstrates that the designed thermo-sensitive formulation is biocompatible with high cell viability (over 96 %). The fabricated hydrogel can be deemed as a potential intelligent drug delivery platform for controlling cutaneous candidiasis due to antifungal effectiveness and less toxicity, which can be a promising alternative to traditional drug delivery systems.

Green synthesis of three-component Ag/AgCl/TiO2 nanocomposite using Zataria Multiflora plant and evaluation of its application in the production of the biodegradable film to increase egg durability in packaging

Green synthesis of three-component Ag/AgCl/TiO2 nanocomposite using Zataria Multiflora plant and evaluation of its application in the production of the biodegradable film to increase egg durability in packaging

Assessment of Zataria Multiflora Essential Oil—Incorporated Electrospun Polyvinyl Alcohol Fiber Mat as Active Packaging

In this study, an active packaging containing Zataria multiflora essential oil (ZMEO), a powerful natural antimicrobial agent, encapsulated into polyvinyl alcohol (PVA) fiber via electrospinning is presented. ZMEO was effective on pathogenic bacteria, particularly Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus, Listeria monosytogene), fungi and yeasts (Aspergillus fumigatus, Candida albicans). Results showed that the scanning electron microscopy (SEM) images of fibers had a bead-free and uniform structure. Fourier-transform infrared (FTIR) revealed that ZMEO was encapsulated into PVA through a physical process, without chemical interaction between the ingredients. Strawberries treated with PVA/ZMEO significantly (p < 0.05) preserved the anthocyanin (18.64%), total phenols (12.95%), antioxidant (22.72%), soluble solids (6.44%), titratable acidity (20.88%), firmness (27.2%), and color (15.55%) compared to the control sample during 15 days of cold storage. According to these findings, electrospinning was an efficient method for encapsulating bioactive compounds. ZMEO loaded into PVA fiber delayed the physiological and biochemical changes of fruits and extended the fruit's shelf-life. This study revealed the benefits of incorporating ZMEO into PVA fiber mats, which could lead to new possibilities for active packaging.

Effect of sage seed gum film incorporating Zataria multiflora Boiss essential oil on the storage quality and biogenic amine content of refrigerated Otolithes ruber fillets

The effect of an edible film based on sage seed gum (SSG) incorporating 3 % Zataria multiflora Boiss essential oil (ZEO) was investigated on the storage quality and shelf life of tiger-tooth croaker (Otolithes ruber) fillets during storage at 4 ± 1 °C compared to the control film (SSG film without ZEO) and the Cellophane. The SSG-ZEO film significantly decelerated microbial growth (evaluated by total viable count, total psychrotrophic count, pH, TVBN) and lipid oxidation (evaluated by TBARS) compared to the others (P ˂ 0.05). The antimicrobial activity of ZEO was the highest and the lowest on E. aerogenes (MIC: 0.196 μL/mL) and P. mirabilis (MIC: 0.977 μL/mL), respectively. E. aerogenes was identified as an indicator biogenic amine-producer in O. ruber fish at refrigerated temperature. The active film significantly lowered biogenic amine accumulation in the samples inoculated with E. aerogenes. A clear relationship was observed between the release of ZEO's phenolic compounds from the active film to the headspace and the reduction of microbial growth, lipid oxidation, and biogenic amine production in the samples. Consequently, SSG film containing 3 % ZEO is proposed as a biodegradable antimicrobial-antioxidant packaging to extend the shelf life and decrease the biogenic amine production in refrigerated seafood.

Studying the microbial, chemical, and sensory characteristics of shrimp coated with alginate sodium nanoparticles containing Zataria multiflora and Cuminum cyminum essential oils.

Retardation of quality loss of seafood has been a new concept in recent years. This study's main objective was to evaluate the microbial, chemical, and sensory attributes of shrimp coated with alginate sodium nanoparticles containing Zataria multiflora and Cuminum cyminum essential oils (EOs) during refrigerated storage. At the end of storage time (15 days storage at 4°C), the pH, thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVBN) amounts in shrimps coated with the alginate nanoparticles were 7.62, 1.14 mg MDA/kg, and 117 mg/100 g which were significantly (p < .05) lower than the control groups. The count of all bacteria groups was also lower in this treatment, which was 2-2.74 Log CFU/mL on day 15 of cold storage. This combined treatment also obtained the highest sensory scores (around 7) and the lowest melanosis score (2.67) due to the effective delaying microbial and oxidation activities. Therefore, this edible coating could substantially retard microbial and chemical changes and improve the organoleptic properties of shrimp under refrigerated storage.

Formulation and Standardization of Herbal Cream for Acne Vulgaris

At some point in their lives, everyone develops acne, pimples, sunburn scars, and pigmentation. In order to treat their skin issues and provide them a good, healthy complexion, customers have started shopping for goods like anti-acne cream. However, the majority of anti-acne creams on the market are loaded with ingredients to which some people may have an adverse reaction. Using two gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two gram-negative bacteria, the extract and cream's antibacterial activities were tested using the disc diffusion method (Escherichia coli and Salmonella enterica). The bactericidal effects of the extract were investigated at dosages of 5, 10, and 25 mg/ml. The extract's antibacterial properties were incredibly ineffectual against all investigated species. As soon as the extract was added to the cream's recipe at Concentrations of 10, 25, and 50 successfully maintained this activity. After two months, the cream's formulation remained the same. After clinical and toxicology testing are complete, this cream formulation can be successfully utilised to treat skin infections, including acne vulgaris. The primary pathogenic factors include androgen-stimulated stimulation of sebaceous gland secretion, hyperkeratinization, abnormal infundibular epithelium keratinization that blocks sebaceous follicles, and Propionibacterium acnes colonisation of the pilosebaceous unit, which causes inflammation around the sebaceous follicles. The common formulations that are already on the market typically have a variety of unfavourable consequences, including poor patient compliance, skin irritation, dryness, peeling, and itching, as well as decreased therapeutic efficiency. A skin condition called acne vulgaris will almost certainly affect everyone at some point in their lives. Herbal therapies are thought to be safer than allopathic ones because they are less likely to have adverse effects such contact allergies, localised irritation, scaling, photosensitivity, itching, pruritus, skin peeling, etc. This study showed that a variety of herbal extracts had anti-acne properties. The cream's recipe included a sizeable amount of poly herbal extract. An in vitro method of measuring antibacterial activity was used to test the anti-acne effects of the suggested formulation (broth dilution method and sub culturing method). Using the skin feel test, the spreadability, greasiness, tackiness, film-forming, softening, soothing, and pleasantness of cream were evaluated. The outcomes demonstrated that the formula chosen also possessed potent anti-acne properties. As a result, we might encourage more in-vivo research and testing on items that are already on the market. Acne is a pilosebaceous-related skin condition that is continuously inflammatory. This condition is widespread and affects 85% of youngsters worldwide. Acne's disfiguring dermatosis might significantly lessen emotional discomfort by weakening self-confidence. Available as topical and systemic therapy for treating acne are comedolytic medicines, antibiotics, and many anti-inflammatory medications. Because of the undesirable side effects, frequently high cost, and antibiotic resistance in acne-causing bacteria, the need for medicinal plants has been gradually rising. This review provides the most recent information on medicinal plants and the phytoconstituents used to treat acne. The most popular anti-acne drugs, like as retinoids, systemic and topical antibiotics, and hormones, have a number of dangerous side effects. According to the literature, Calendula officinalis, Rosa canina, Zataria multiflora, Trigonal foeman graecum, and Glycine max all have antibacterial, anti-inflammatory, and antioxidant qualities that could be used to treat the disease's inflamed sores. Before making changes, a standard washing cream recipe containing bees wax, spermaceti, borax, liquid paraffin, and water was considered.

The impact of Zedo gum based edible coating containing Zataria multiflora Boiss essential oil on the quality enhancement and shelf life improvement of fresh buffalo meat

The impact of Zedo gum based edible coating containing Zataria multiflora Boiss essential oil on the quality enhancement and shelf life improvement of fresh buffalo meat