Xiamen University Research Articles

The people behind the papers - Xin Zhu, Yiquan Wang and Guang Li.

During development, the establishment of directional left-right (L-R) asymmetry is crucial for the correct positioning of organs within the body. How symmetry is broken in the embryo is still incompletely understood at the molecular level, as is its evolutionary history. A new paper in Development tackles this problem with an analysis of L-R asymmetry in the basal chordate amphioxus. We caught up with first author Xin Zhu and supervisors Yiquan Wang and Guang Li of Xiamen University in Fujian, China, to find out more about the work.

Preface

It is our great pleasure to welcome you to 2019 International Conference on Resources and Environmental Research (ICRER 2019) which was successfully held in Shandong University, Qingdao, China during October 25-27, 2019. ICRER 2019 is co-organized by Shandong University, assisted by Xiamen University of Technology, South-South Collaborative and Sustainable Development Center, International Society for Environmental Information Sciences (ISEIS) and Shandong University of Technology, Hong Kong Chemical, Biological & Environmental Engineering Society (HKCBEES), Environment and Agriculture Society (EAS).ICRER 2019 is dedicated to issue related to resources and environmental research. The major goal and feature of the conference is to bring academic scientists, engineers, industry researchers together to exchange and share their experiences and research results, and discuss the practical challenges encountered and the solutions adopted. Prof. Shuguang Wang from School of Environment Science and Engineering, Shandong University, China has been invited to do the welcome address; Prof. Edward McBean from University of Guelph, Canada has shared his keynote speech” Challenges for the Future, for Sponge City”; Prof. Yongping Li from Beijing Normal University, China has presented her keynote speech “Sustainable water resources management under uncertainty – A case study of Central Asia”; Prof.Zhijun Peng from University of Bedfordshire, UK has given his keynote speech” The Way to Save CO2 Emissions with BEVx (Plug-in Pure Battery Electric Vehicles)”; Prof. Christophe Guimbaud from Université Orléans, France has presented his keynote speech” Impact of global changes on Greenhouse Gas (GHG) exchanges with atmosphere for sphagnum type peatlands: field study and modelling approach for methane emissions”; Dr. Pengfei Xia from Center for Applied Geosciences, University of Tübingen, Tübingen, Germany has given his keynote speech” Harnessing synthetic biology for recycling carbon dioxide”; Prof. Gordon Huang from University of Regina, Canada has shared his keynote speech “The Way to Save CO2 Emissions with BEVx (Plug-in Pure Battery Electric Vehicles).Many researchers, engineers, academicians as well as industrial professionals from all over the world have presented their research results and development activities. There were three topics for all the session presentations: Modeling of energy management systems, Energy and environmental Studies, Technologies of power and energy engineering. It will be a golden opportunity for the students, researchers and engineers to interact with the experts and specialists to get their advice or consultation on technical matters, sales and marketing strategies.This conference proceeding presents a selection from papers submitted to the conference from universities, research institutes and industries. All of the papers were subjected to peer-review by conference committee members and international reviewers. The papers selected depended on their quality and their relevancy to the conference. The volume tends to present to the readers the recent advances in the field of resources and environmental research and various related areas.We would like to thank all the authors who have contributed to this volume and also to the organizing committees, reviewers, speakers, chairpersons, sponsors and all the conference participants for their support to ICRER 2019.Prof. Shuguang WangSchool of Environment Science and Engineering, Shandong University, China November 25,2019

Electric-Field-Induced Connectivity Switching in Single-Molecule Junctions.

SummaryThe manipulation of molecule-electrode interaction is essential for the fabrication of molecular devices and determines the connectivity from electrodes to molecular components. Although the connectivity of molecular devices could be controlled by molecular design to place anchor groups in different positions of molecule backbones, the reversible switching of such connectivities remains challenging. Here, we develop an electric-field-induced strategy to switch the connectivity of single-molecule junctions reversibly, leading to the manipulation of different connectivities in the same molecular backbone. Our results offer a new concept of single-molecule manipulation and provide a feasible strategy to regulate molecule-electrode interaction.

Label-Free Visualization of Early Cancer Hepatic Micrometastasis and Intraoperative Image-Guided Surgery by Photoacoustic Imaging.

The detection of cancer micrometastasis for early diagnosis and treatment poses a great challenge for conventional imaging techniques. The aim of our study was to evaluate the performance of photoacoustic imaging (PAI) in detecting hepatic micrometastases from melanoma at a very early stage and in aiding tumor resection by intraoperative guidance. Methods: In vivo studies were performed by following protocols approved by the Ethical Committee for Animal Research at Xiamen University. First, a mouse model of B16 melanoma metastatic to the liver (n = 10) was established to study the development of micrometastases in vivo. Next, the mice were imaged by a scalable PAI instrument, ultrasound, 9.4-T high-resolution MRI, PET/CT, and bioluminescence imaging. PAI scans acquired with optical wavelengths of 680-850 nm were kept spectrally unmixed by using a linear least-squares method to differentiate various components. Differences in signal-to-background ratios among different modalities were determined with the 2-tailed paired t test. The diagnostic results were assessed with histologic examination. Excised liver samples from patients diagnosed with hepatic cancer were also examined to identify the tumor boundaries. Surgical removal of metastatic melanoma was precisely guided in vivo by the portable PAI system. Results: PAI was able to detect metastases as small as approximately 400 μm at a depth of up to 7 mm in vivo-a size that is smaller than can be detected with ultrasound and MRI. The tumor-to-liver ratio for PAI at 8 d (4.2 ± 0.2, n = 6) and 14 d (9.2 ± 0.4, n = 5) was significantly higher than for PET/CT (1.8 ± 0.1, n = 5, and 4.5 ± 0.2, n = 5, respectively; P < 0.001 for both). Functional PAI revealed dynamic oxygen saturation changes during tumor growth. The limit of detection was approximately 219 cells/μL in vitro. We successfully performed intraoperative PAI-guided surgery in vivo using the portable PAI system. Conclusion: Our findings offer a rapid and effective complementary clinical imaging application to noninvasively detect micrometastases and guide intraoperative resection.

Multi-Factor Analysis of Single-Center Asthma Control in Xiamen, China.

We evaluated the effects of air pollutants, age, allergic history, family allergic history, treatment, treatment steps, and compliance on uncontrolled childhood asthma in Xiamen, China. The clinical data of children with asthma in the pediatric outpatient department of the First Affiliated Hospital of Xiamen University from January 2016 to June 2018 were analyzed retrospectively. According to the assessment of the patients' outcome including well-controlled, partly-controlled and uncontrolled, 7,211 cases of 3,268 patients were selected. Rank sum test and ordered multi-class logistic regression analysis were used. In the rank sum test, age, allergic history, family allergic history, season, treatment, treatment steps and compliance were found associated with uncontrolled rate (all P < 0.001). Logistic regression analysis showed that PM10, NO2, and SO2 raised uncontrolled-asthma rate (aOR 1.311, aOR 1.281, aOR 1.252, respectively). Older children had lower uncontrolled rate (OR = 0.849, 95% CI: 0.758–0.950), children with higher treatment steps had higher uncontrolled rate (OR = 1.227, 95%CI: 1.040–1.448), and children with better treatment compliance have lower uncontrolled rate (OR = 0.374 95% CI: 0.331–0.424). The order of the uncontrolled rate of asthma from high to low was winter, spring, autumn, and summer. PM10, NO2, SO2, age, season, treatment steps, and treatment compliance have significance for predicting the control rate of childhood asthma in Xiamen, China.

Combining 3D heads-up display viewing system and intraoperative optical coherence tomography-assisted vitrectomy for myopic foveoschisis

Objective To observe the clinical efficacy of digital 3D heads-up display viewing system(3D viewing system) and intraoperative OCT (iOCT) in vitrectomy for myopic foveoschisis (MF). Methods A retrospective, consecutive case series. From October 2018 to May 2019, Nineteen eyes of 19 consecutive patients with MF diagnosed in Xiamen Eye Center of Xiamen University who underwent vitrectomy were included in this study. There were 7 males and 12 females, with the mean age of 54.47±11.38 years. The average axial length was 30.40±2.30 mm, the mean logMAR BCVA was 0.56±0.31, the mean central foveal thickness (CFT) was 317.80±151.9.32 μm, the mean max retinal thickness (maxRT) was 556.7±143.7 μm. All the surgeries performed combined with 3D viewing system with iOCT. The standard 25G pars planar vitrectomy were performed with removing the posterior vitreous and indocyanine green (ICG) staining of internal limiting membrane (ILM) and air-fluid exchange. Thirteen of 19 eyes underwent fovea-sparing ILM peeling and the other 6 eyes not. The average follow-up was 4.2±1.4 months. All the patients were on regular follow-up to document the changes on BCVA, anatomical changes in macula, CFT and maxRT. Paired t test was used to compare BCVA, CFT and maxRT before and after surgery. Results The fine images of macula were clearly shown on the 3D viewing system in all eyes. The electronic green filter enhanced the contrast sensitivity of ICG stained images. Clear images of macula were captured by iOCT in all eyes. The average surgical time was 35.5±8.2 min. On the last follow-up, 16 of 19 eyes with MF resolved. The mean CFT was 178.5±103.5 μm, the maxRT was 341.8±83.8.16 μm, and the mean logMAR BCVA was 0.35±0.22. The differences of CFT, maxRT and logMAR BCVA before and after surgery were statistically significant (t=4.181, 7.154, 5.129; P<0.001). Minimal invisible full thickness macular hole were detected in 2 eyes by iOCT and repaired with auto serum or ILM flap covering. There was no complication associated with the 3D viewing system. Conclusions 3D viewing system provides improved contrast and crystal clear macular image stain with ICG in pathological myopia. iOCT can detect the minimal invisible full thickness macular hole during surgery. Both may contribute to improved MF closure rate and BCVA. Key words: Vitreoretinal surgery/instrumentation; Tomography, optical coherence; Retinoschisis/surgery; Myopiadegenerative/complications; 3D heads-up display viewing system

Expressions of plasma D-dimer, von Willebrand factor antigen and blood coagulation factor VII in lymphoma and their clinical significances

Objective To investigate the clinical value of blood coagulation and fibrinolysis index detection in lymphoma patients. Methods A total of 115 lymphoma patients hospitalized at Zhongshan Hospital of Xiamen University from January 2013 to September 2017 were retrospectively analyzed. According to the diagnostic and therapeutic criteria of lymphoma from World Health Organization (2008), these patients were divided into chemotherapy remission group (76 cases) and chemotherapy non-remission group (39 cases). A total of 138 healthy examination subjects at the same period were selected as the control group. Coagulation factor Ⅶ (FⅦ), D-dimer (D-D), von Willebrand factor antigen (vWF: Ag) and serum lactate dehydrogenase (LDH) levels were measured in all subjects. Kruskal-Wallis test was used to compare the differences of blood coagulation and fibrinolysis indicators in patients with different lymphoma staging and stratified treatment outcomes. Correlation test of D-D and LDH and disease staging was performed by using Spearman correlation analysis. The receiver operating characteristics (ROC) curve was used to analyze the efficacy of D-D in the assisted diagnosis of lymphoma with thrombosis. Results The plasma D-D, vWF: Ag levels and FⅦ activity [the median (interquartile range)] in lymphoma patients were higher than those in healthy controls [1 240 ng/ml (1 610 ng/ml) vs. 250 ng/ml (43 ng/ml), Z = -10.728, P < 0.01; 170 ng/ml (113 ng/ml) vs. 105 ng/ml (28 ng/ml), Z = -6.425, P < 0.01; 120% (26%) vs. 96% (26%), Z = -4.602, P < 0.01]. With the increase of Ann Arbor stage, plasma D-D, vWF: Ag levels and FⅦ activity were also increased gradually (all P < 0.05); plasma D-D, vWF: Ag levels and FⅦ activity in lymphoma with thrombosis group were higher than those in the group without thrombosis (all P < 0.01), D-D and vWF: Ag levels in the chemotherapy remission group were lower than those in the chemotherapy non-remission group (all P < 0.01). Plasma D-D levels were positively correlated with LDH level and Ann Arbor stage (r values were 0.414 and 0.530, respectively, all P < 0.01). When the plasma D-D level was 1 735 ng/ml, the sensitivity of diagnosis of thrombosis in patients with lymphoma was 81.8%, the specificity was 85.7%, the area under the ROC curve was 0.894, and the Youden index was the highest (0.675). Conclusions Clinically, blood coagulation and fibrinolysis in patients with lymphoma can be evaluated by detecting blood coagulation and fibrinolysis indexes such as plasma D-D. Real-time monitoring of plasma D-D level can determine the thrombosis trend of lymphoma patients, and it may play an important role in evaluating the efficacy and prognosis. Key words: Lymphoma; D-dimer; von Willebrand factor antigen; Blood coagulation factor Ⅶ

Neonatal capillary leak syndrome: analysis of 68 cases

Objective To analyze clinical features, treatment, prognosis and risk factors for death of capillary leak syndrome (CLS) in neonates. Methods This retrospective study involved 68 neonates with CLS treated in the Department of Neonatology, Women and Children's Hospital, School of Medicine, Xiamen University from January 2013 to December 2017. Clinical data, including features, causes, treatment and outcomes of those CLS cases were analyzed. Chi-square test and multivariate logistic regression analysis were performed. Results Among the 68 cases consisting of 49 males and 19 females, 86.7% (59/68) were born at ≥ 35 gestational weeks. Fifty-three neonates (77.9%) developed symptoms within three days after admission. Forty-two cases (61.8%) had respiratory distress syndrome and 35 (51.5%) had septicemia. The mortality rate was 23.5% (16/68). Among the survivors, 38.5% (20/52) showed abnormal cranial MRI. Univariate analysis with Chi-square test showed that neonatal death due to CLS was associated with the lactic acid level >10 mmol/L, oliguria lasting for 12 h or anuria for 8 h, no negative fluid balance occured within seven days, adrenaline infusion >0.6 μg/(kg·min) and administration of 3% sodium chloride. Multivariate logistic regression analysis showed that lactic acid level, oliguria/anuria duration and the time achieve negative fluid balance were independent risk factors for neonatal death of CLS. Conclusions Neonatal CLS is a condition with high fatality rate and poor prognosis. Respiratory distress syndrome and septicemia are the common causes. The prognosis of CLS might be improved by treatment with 3% sodium chloride. Lactic acid level, oliguria/anuria duration and the time achieve negative fluid balance are independent risk factors for neonatal death due to CLS. Key words: Capillary leak syndrome; Death; Risk factors; Infant, newborn

An odyssey: the way Chinese catalysis towards the world

The 16 th International Catalysis Congress (16th ICC) was successfully held in China in July 2016. This paper reviewed the development of catalytic science and technology of China from scratch to small then to large under the efforts of several generations. In 1950s, catalysis discipline was first set up to train early catalytic professionals in Jilin University, Peking University and Xiamen University. Subsequently, a large research team was formed in colleges and universities, the Academy of Sciences, the Enterprise Research Institute to carry out a large number of catalytic researches. Along with the Chinese reform and opening-up, the spring of science came, and the state started to emphasize and strongly support scientific research. Chinese catalytic researchers began to enter the international catalytic academic exchange platform. Famous foreign scientists are invited to visit China and a large number of visiting scholars and foreign students have been sent to the United States, Europe and Japan, many of them have become well-known professors, and grown into catalytic academic elites. The first China-Japan-USA Symposium on Catalysis was held in Dalian in 1982, and it was expanded to become the Asian-Pacific Congress on Catalysis (APCAT), one of the three regional catalytic conferences in the world. After several generations of bidding for the organization of the International Catalytic Congress three times, China won the right to host the 16th ICC. It has effectively promoted the Chinese catalytic academic circles to the international academic ones and improved the influence of catalysis communities in China significantly. The great development in catalytic research and technology has condensed the efforts of several generations of catalysts. To make China a catalytically strong country, there is still a long way to go. We hope that the contemporary scientists can accomplish this historical task. With the careful planning and arrangements by the Chinese catalytic elites, the quadrennial grand event in the international catalytic world—the 16 th International Congress on Catalysis (ICC) was successively held in Beijing presentations indicating the frontiers of the catalytic science and the magnanimity of the organizers. This paper reviewed the development of catalytic science and technology of China from scratch to small then to large under the efforts of several generations.

Depolarization field in relaxor-based ferroelectric single crystals under one-cycle bipolar pulse drive**Project supported by the National Natural Science Foundation of China (Grant No. 11674270), the Fundamental Research Funds for Xiamen University, China (Grant No. 20720180113), the Education and Scientific Research Project for Young and Middle-aged Teachers of Fujian Province, China (Grant No. JAT170036), the Opening Fund of Acoustics Science and

The [001]c-polarized (1 − x)Pb(Mg1/3Nb2/3)O3–xPbTiO3 (PMN−PT) single crystals are widely used in ultrasonic detection transducers and underwater acoustic sensors. However, the relatively small coercive field (∼ 2 kV/cm) of such crystals restricts their application at high frequencies because the driving field will exceed the coercive field. The depolarization field can be considerably larger in an antiparallel direction than in a parallel direction with respect to polarization when the bipolar driving cycle starts. Thus, if the direction of the sine wave signal in the first half cycle is opposite to the polarization direction, then the depolarized domains can be repolarized in the second half of the sine cycle. However, if the direction of the sine wave signal in the first half of the cycle is along the polarization direction, then the change is negligible, and the domains switched in the second half of the sine cycle cannot be recovered. The design of electric driving method needs to allow the use of a large applied field to emit strong enough signals and produce good images. This phenomenon combined with the coercive field increases with the driving frequency, thereby making the PMN−PT single crystals usable for high-frequency applications. As such, the applied field can be considerably larger than the conventionally defined coercive field.

Preface

It is our great pleasure to welcome all of you to 2019 International Conference on Environment and Ocean Engineering (ICEOE 2019) which was held on June 7-9, 2019 in Xiamen Ocean Vocational College, Xiamen, China. ICEOE 2019 is dedicated to issues related to Environment and Ocean Engineering.ICEOE 2019 is organized by Xiamen Ocean Vocational College and Xiamen University of Technology, assisted by International Society for Environmental Information Sciences (ISEIS), Hong Kong Chemical, Biological & Environmental Engineering Society (HKCBEES), Environment and Agriculture Society (EAS), Fujian Smart City Association. ICEOE 2019 is highlighted by several professional speakers. Prof. Caterina Valeo from University of Victoria, Canada; Prof. Yongping Li from Xiamen University Of Technology, China; Prof. Lixiao Zhang form School of Environment, Beijing Normal University, China; Prof. Guangwei Huang from Sophia University, Tokyo, Japan; Prof. R. J. (Dick) Haynes from The University of Queensland, St Lucia, Queensland, Australia; Prof. Gordon Huang from University of Regina, Canada had attended the conference as keynote speakers.There were five sub-sessions with different topics: Modeling of Environmental Systems, Environmental Pollution Control, Environmental Management and Planning, Workshop on Environment, Water and Energy, Forum on South-South Sustainable Development. It was a golden opportunity for students, researchers and engineers to interact with the experts and specialists to get their advice or consultation on technical matters, dissemination and marketing strategies.This proceeding presents a selection from papers submitted to the conference from universities, research institutes and industries. It has five chapters with topic: Coastal Engineering, Deep Sea Engineering, Ship Engineering, Utilization of Marine Resources, Water Pollution Control. All papers were subjected to peer-review by conference committee members and international reviewers. The papers were selected based on high quality and relevancy to the conference scope.We would also express our sincere gratitude to organizing committee and the volunteers who had dedicated their time and efforts in planning, promoting, organizing and helping the conference.Changping ChenXiamen Ocean Vocational College, China2019-09-20

Clinical analysis and classification of placental abruption

Objective To investigate the diagnosis, treatment, and maternal and fetal outcomes of placental abruption. Materials and methods We recruited 585 cases of placental abruption from the Women and Children’s Hospital Affiliated to Xiamen University between January 2012 and December 2017. Cases were categorized into four groups (class 0–III) according to the clinical guidelines published by the Obstetrics and Gynecology Branch of the Chinese Medical Association. We then compared clinical data and auxiliary examinations across the four groups. Results The differences were statistically significant (p < .01) among the four groups of placental abruption with regard to the incidence of an abnormal ultrasound finding. Positive ultrasound signs were evident in 6.4% of the patients categorized as class 0 and 100.0% of patients categorized as class III. Monitoring showed that fetal heart rate (FHR) was abnormal in class II patients with placental abruption; patients in class III showed no fetal heart sounds. Cesarean section was carried out for 26.6%, 75.1%, 65.2%, and 47.1% of patients in classes 0, I, II, and III, respectively. The rate of cesarean section for classes I and II was the highest, while the lowest rate occurred for class 0. Postpartum hemorrhage occurred in 2.5%, 9.3%, 15.2%, and 29.4% of patients across the four groups, DIC occurred in 0.0%, 1.3%, 2.3%, and 23.5% of cases, and perinatal death occurred in 1.0%, 1.3%, 7.6%, and 100.0% of cases, respectively. The highest incidence of postpartum hemorrhage was in class III (29.4%) and the lowest was in class 0 (2.5%). The highest incidence of DIC was in class III (23.5%) and the lowest was in class 0 (0.0%). The highest incidence of neonatal asphyxia was in class II (34.1%) and the lowest was in class 0 (10.1%). Regarding perinatal death, the highest incidence was in class III (100.0%) and the lowest was in class 0 (1.0%). These data showed significance differences when compared across the four groups of patients (p < .01). Conclusions We recommend that the diagnosis of placental abruption should consider risk factors, clinical features, FHR monitoring, and dynamic ultrasound monitoring. Early diagnosis and treatment can improve maternal and infant prognosis.

Preface

It is our great pleasure to welcome you to 2019 10th International Conference on Environmental Science and Technology (ICEST 2019) which was successfully held in Xiamen Ocean Vocational College, Xiamen, China during June 7-9, 2019.ICEST 2019 is co-organized by Xiamen Ocean Vocational College and Xiamen University of Technology, assisted by International Society for Environmental Information Sciences (ISEIS), Hong Kong Chemical, Biological & Environmental Engineering Society (HKCBEES), Environment and Agriculture Society (EAS), Fujian Smart City Association.ICEST 2019 is dedicated to issues related to environmental science and technology. The major goal and feature of the conference is to bring academic scientists, engineers, industry researchers together to exchange and share their experiences and research results, and discuss the practical challenges encountered and the solutions adopted. Prof. Changping Chen from Xiamen Ocean Vocational College, China gave the opening remark; Prof. Caterina Valeo from University of Victoria, Canada gives her keynote speech” Scaling in Sustainable Urban Design”; Prof. Yongping Li from Xiamen University of Technology, China gives her keynote speech “Water-energy-food nexus: Integrated simulation-optimization approach”; Prof. Lixiao Zhang form School of Environment, Beijing Normal University gives his keynote speech” Food-Energy-Water Nexus for Urban Sustainability: Conceptual Framework and Real Challenges”; Prof. Guangwei Huang from Sophia University, Tokyo, Japan gives his keynote speech” Shallow Lake Restoration: Case Studies in Japan”; Prof. R. J. (Dick) Haynes from The University of Queensland, St Lucia, Queensland gives his keynote sppech” Sustainable revegetation of bauxite processing residue”; Prof. Gordon Huang from Faculty of Engineering and Applied Science, University of Regina, Canada gives his keynote speech “Management of Watershed Environmental Risks”.Many researchers, engineers, academicians as well as industrial professionals from all over the world have presented their research results and development activities. There were five sessions: Modeling of Environmental Systems, Environmental Pollution Control, Environmental Management and Planning, Workshop on Environment, Water and Energy, Forum on South-South Sustainable Development. It will be a golden opportunity for the students, researchers and engineers to interact with the experts and specialists to get their advice or consultation on technical matters, sales and marketing strategies.

632P - Patient-derived xenografts (PDX) identify JMJD6 inhibitor as an effective therapeutic medicine in colorectal cancer

BackgroundThe Jmjc-domain-containing protein 6 (JMJD6) is a member of JmjC oxygenases that contain both arginine demethylase and lysine hydroxylase activities. Overexpression of JMJD6 has been reported to be associated with the development of various types of cancers, such as breast, lung, liver, and colon cancer. The present study aimed to explore the inhibitory effect of the small-molecule JMJD6 inhibitor named WL8 obtained by virtual screening on the progression of colorectal cancer. MethodsWL8 was administered to a mouse bearing xenografts of human colorectal cancer tissue, once every 7 days for 3 times. We analyzed the response of tumors from three patients to the JMJD6 inhibitor (11.5mg/kg, intrapertoneally, i.p.), and to FOLFOX (oxaliplatin 12mg/kg, calcium levifolinate 30mg/kg, 5-fluorouracil 55mg/kg, i.p.) or FOLFIRI (irinotecan 40mg/kg, calcium levifolinate 30mg/kg, 5-fluorouracil 55mg/kg, i.p.), as well as to combined usage of JMJD6 inhibitor and FOLFOX or FOLFIRI in PDX models by recording the tumors sizes every 3 or 4 days for 7 times. The differences between groups were analyzed using one-way ANOVA. (i.p. stands for intraperitoneal.). ResultsCompared with FOLFOX or FOLFIRI, WL8 inhibited tumor growth more effectively in the PDX models of three patients (One patient’s partial results are shown in the table). Combinatorial therapies using both JMJD6 and FOLFOX (or FOLFIRI) could further reduced the tumour size.Table: 632PTable: 632PGroup8 Days15 Days22 DaysControl1.8075±0.3950 $^@2.5706±0.2900 #%$^@2.6834±0.0416 #%$^@FOLFOX1.4184±0.20671.3639±0.2381 *@1.3867±0.4155 *^@FOLFIRI1.4712±0.15681.3894±0.4543 *@1.7474±0.0705 *$^@WL80.9656±0.4456 *1.1069±0.4376 *1.1655±0.2884 *%^@WL8+ FOLFOX0.7125±0.0410 *#%0.7767±0.4359 *0.4833±0.0503 *#%$WL8+ FOLFIRI1.0325±0.3903*0.6367±0.1436 *#%0.3900±0.0265 *#%$Relative tumor volumn (on days after first administration, notes: P values are indicated with symbols as follows, * P<0.05 v.s. ctrl; # P<0.05 v.s. FOLFOX; % P<0.05 v.s. FOFIRI; $ P<0.05 v.s. WL8; ^ P<0.05 v.s. WL8+FOLFOX; @ P<0.05 v.s. WL8+FOLFIRI) ConclusionsWe found an small-molecule inhibitor of JMJD6 with impressive therapeutic effects on colorectal cancer in pre-clinical study. Legal entity responsible for the studyThe First Affiliated Hospital of Xiamen University. FundingNational Natural Science Foundation of China, Fujian Provincial Department of Science and Technology, Fujian Provincial Health and Family Planning Commission Foundation of Youth Scientific Research Project, Xiamen Science and Technology Bureau Foundation of Science and Technology Project for the Benefit of the People. DisclosureAll authors have declared no conflicts of interest.

Predictive value of prostate biopsy results based on predictive model established by the PI-RADS version 2

Objective To explore a predictive nomogram for the result of prostate biopsy based on Prostate Imaging Reporting and Data System version 2(PI-RADS v2)combined with prostate specific antigen (PSA) and its related parameters, and to assess its ability to diagnose prostate cancer by internal validation. Methods We retrospectively analyzed the clinical data of 509 patients who underwent transrectal prostate biopsy guided by ultrasound during the period from January 2014 to December 2018 in the Department of Urology, First Affiliated Hospital of Xiamen University. In 509 cases, the mean age was (68.1±7.2) years. The mean prostate volume(PV) was (55.8±30.7) ml. The mean tPSA value was (19.86±18.94) ng/ml. The mean value of fPSA was (2.63±3.60) ng/ml and the mean f/tPSA was 0.14±0.08. The mean PSAD was (0.46±0.52) ng/ml2. Based on the PI-RADS v2, score 1 point have 37 cases, score 2 point have 131 cases, score 3 point have 152 cases, score 4 point have 102 cases, score 5 point have 87 cases. Of these patients, we randomly selected 80% (407 cases) as development group, and the other 20% (102 cases) as validation group. Univariate and multivariate logistic regression analysis of the development group was performed to identify the independent influence factors that can predict prostate cancer (PCa), thereby establishing a predictive model for the result of prostate biopsy. In the development group, validation group and tPSA was between 4.1-20.0 ng/ml, the model was evaluated by analyzing the receiver operating characteristic (ROC) curve, calibration curve and decision curve, and compared to PSA, fPSA, f/tPSA, PSAD, PI-RADS v2. Results Among the 509 patients enrolled in the study, the detection rate of PCa was 43.0% (219/509). In the development group, the logistic regression analysis demonstrated that patient age (OR=1.113), f/tPSA (OR=0.004), PV (OR=0.986), PSAD (OR=11.023), digital rectal examination (DRE) texture (OR=2.295), transabdominal ultrasound (TAUS) with or without hypoechoic (OR=2.089), and PI-RADS v2 (OR=1.920) were independent factors for PCa (P<0.05). The nomogram based on all variables was established. In the development group, the area under the curve (AUC) of the model (0.883) was greater than those of tPSA (0.686), fPSA (0.593), f/tPSA (0.626), PSAD (0.777), PI-RADS v2 (0.761). In the validation group, the area under the curve of the model (0.839) was greater than those of tPSA (0.758), fPSA (0.666), f/tPSA (0.648), PSAD (0.832), PI-RADS v2 (0.803). In patients whose tPSA was between 4.1-20.0 ng/ml, the area under the curve of the model (0.801) was greater than those of tPSA (0.570), fPSA (0.426), f/tPSA (0.657), PSAD (0.707), PI-RADS v2 (0.701). The calibration curve of the nomogram indicated that the prediction curve was basically fitted to the standard curve, and the Hosmer-Lemeshow showed thatχ2=5.434, P=0.710, both suggested that the prediction model had better calibration ability. The decision curve showed that the model based on PI-RADS v2 had high clinical application value. Conclusions The nomogram based on PI-RADS v2 had a high predictive value for prostate cancer and could significantly improve the diagnostic performance. It had better diagnostic value than PSA and its related parameters. It also provided important guidance for the prostate cancer on clinical treatment of patients to some extent. Key words: Prostatic neoplasms; Prostate imaging reporting and data system version 2; Model; Nomogram

Sliding compression technique with proximal femoral nail antirotation for intertrochanteric fractures in the elderly patients

Objective To observe the clinical efficacy of sliding compression technique with proximal femoral nail antirotation (PFNA) for the treatment of intertrochanteric fractures in the elderly patients. Methods A retrospective study was conducted in the 97 elderly patients with intertrochanteric fracture who had received PFNA internal fixation at Department of Orthopaedics, The Second Fuzhou Hospital Affiliated to Xiamen University from January 2010 to December 2017. They were divided into 2 groups depending on whether the sliding compression technique was used or not in the PFNA fixation. In the sliding compression group of 51 patients, there were 31 males and 20 females with an age of 79.9±6.2 years, and 2 cases of type Ⅰ, 8 cases of type Ⅱ, 21 cases of type Ⅲ, 16 cases of type Ⅳ, 3 cases of type Ⅴ and one case of type Ⅵ according to the modified Evans classification. In the conventional PENA group of 46 cases, there were 27 males and 19 females with an age of 77.5±8.0 years, and 3 cases of type Ⅰ, 6 cases of type Ⅱ, 15 cases of type Ⅲ, 18 cases of type Ⅳ and 4 cases of type Ⅴ according to the modified Evans classification. The 2 groups were compared in terms of the separation of fracture ends at immediate postoperation and 4 weeks postoperation, anesthesia time, operative time, intraoperative bleeding, fracture reduction, fracture healing time, Harris scores of hip function at one year postoperation and postoperative complications. Results There were no significant differences between the 2 group of patients in their preoperative general data, indicating they were compatible (P>0.05). The sliding compression group had significantly shorter separation of fracture ends at immediate postoperation and at 4 weeks postoperation (2.18±0.59 mm and 0.92±0.27 mm) and significantly shorter fracture healing time (5.2±0.9 weeks) than the conventional PENA group (3.91±0.76 mm and 2.15±0.52 mm; 6.5±0.9 weeks) (P 0.05). The sliding compression group had significantly lower incidences in pulmonary infection [9.8% (5/51)], blade loosening or cutout [0 (0/51)] and incision complications [0 (0/51)] than the conventional PENA group [26.1% (12/46), 17.4% (8/46) and 19.6% (9/46), respectively] (P<0.05). Conclusions In the treatment of intertrochanteric fractures in the elderly people, sliding compression technique with PFNA may lead to fewer postoperative complications and faster fracture healing. Key words: Hip fractures; Fracture fixation, intramedullary; Bone nails; Aged; Sliding compression

Development of Steatohepatitis and Fibrosis in Chronic HBV Infection Is Linked to Inflammatory Responses Mediated by IL-13 and CCL11

Background: In view of the importance of chronic HBV (CHB) infection and the global burden of non-alcoholic fatty liver disease (NAFLD), it is imperative to understand the potential interplay between the two diseases. Methods: Here, we retrospectively investigated the association between NAFLD and CHB infection in the context of liver fibrosis. Among the 522 consecutive CHB patients who underwent transient elastography between year 2013 and 2016, we studied 449 subjects in the current investigation. Findings: CAP and LSM scores were generally higher in patients with steatosis and fibrosis or cirrhosis. Antiviral treatment had significantly reduced the HBV viral load. Other liver function markers showed a significant positive correlation with both CAP and LSM scores. Plasma IL-13 was independently associated with increased CAP score where every increase of 1 unit of IL-13 was associated with an increase in CAP score by 0.98 unit. CCL11 was independently associated with LSM with every increase of CCL11 by a unit that, in turn, was associated with an increase of LSM score. Interpretations: Together, we found that there was a high concurrence of NAFLD among patients with CHB. The presence of metabolic syndrome and chronic inflammation in CHB patients were two independent factors that led to progression of liver cirrhosis, with IL-13 playing the key role in linking the metabolic with the inflammatory components. Plasma markers of liver steatosis and fibrosis progression are key to development of cell-targeted therapies exploiting specific molecular pathways. Funding Statement: This work was supported by a grant from the Frontier Research Grant (FRG), FG019-17AFR to Mohamed Rosmawati and Xiamen University Malaysia Research Funding (XMUMRF), XMUMRF/2018-C2/ILAB/0001. Marie Larsson is supported by the Swedish Research Council, the Swedish Physicians against AIDS Research Foundation; VINNMER for Vinnova, Linkoping University Hospital Research Fund, ALF Grants Region Ostergotland, FORSS. Declaration of Interests: The authors declare no conflicts interest. Ethics Approval Statement: The medical records of patients were extracted from the hospital database using patients’ unique hospital registration number. This study was approved by the Medical Ethics Committee of the University Malaya Medical Centre (MEC-938.42).

IRF4 and STAT3 Play a Role in the Imbalanced Differentiation of T-Cells in Responses to Inhalable Particulate Matters in Human

Background: Particulate Matter (PM) is known to cause inflammatory responses in human. Although prior studies verified the immunogenicity of PM in cell lines and animal models, the effectors of PM exposure in the respiratory system and the regulators of the immunogenicity of PM is not fully elucidated. Methods: We performed gene-expression profiling of peripheral blood mononuclear cells from 171 healthy subjects in northern China to identify co-expressed gene modules associated with PM exposure. We inferred transcription factors regulating the co-expression and validated the association to T-cell differentiation in both primary T cells and mice treated with PM. Findings: We report two transcription factors, IRF4 and STAT3, as regulators of the gene expression in response to PM exposure in human. We confirmed that the activation of IRF4 and STAT3 by PM is strongly associated with imbalanced differentiation of T-cells in the respiratory tracts in a time-sensitive manner in mouse. We also verified the consequential inflammatory responses of the PM exposure. Moreover, we show that the protein levels of phosphorylated IRF4 and STAT3 increase with PM exposure. Interpretation: Our study suggests IRF4 and STAT3 are potential effectors of PM exposure and regulators of the inflammation in the respiratory tracts. Funding Statement: Natural Science Foundation of Fujian Province (No. 2016J01644), National Natural Science Foundation of China (No. 31371289), and Science and Technology Bureau of Xiamen (NO. 3502Z20189013). Declaration of Interests: The authors declare no competing financial interests. Ethical Approval Statement: This study protocol and informed consent form were overseen and approved by a steering committee, institutional review boards of Xiamen University and the First Affiliated Hospital of Xiamen University. All methods were carried out in accordance with the relevant guidelines and regulations.

Multiple trapping using a focused hybrid vector beam**Project supported by the National Natural Science Foundation of China (Grant Nos. 11604050, 91636109, 61575041, and 61875242), the Fundamental Research Funds for the Central Universities at Xiamen University, China (Grant No. 20720190057), the Natural Science Foundation of Fujian Province of China for Distinguished Young Scientists (Grant No. 2015J06002), the Program for New Century

We propose a simple and efficient method that uses a single focused hybrid vector beam to confine metallic Rayleigh particles at multiple positions. We study the force mechanisms of multiple trapping by analyzing the gradient and scattering forces. It is observed that the wavelength and topological charges of the hybrid vector beam regulate the trapping positions and number of optical trap sites. The proposed method can be implemented easily in three-dimensional space, and it facilitates both trapping and organization of particles. Thus, it can provide an effective and controllable means for nanoparticle manipulation.

Clinical analysis of primary hyperthyroidism complicated with thyroid carcinoma

Objective To analyze the clinicopathological features of primary hyperthyroidism complicated with thyroid carcinoma. Methods From January 2010 to July 2018, 418 patients complicated with thyroid cancer underwent thyroidectomy in the First Affiliated Hospital of Xiamen University were retrospectively analyzed.According to thyroid function, 418 patients were divided into two group: A group(n=39) had hyperthyroidism with thyroid carcinoma, and B group(n=379) had normal thyroid function.The clinicopathological features of the two groups were analyzed and compared. Results There were no statistically significant differences in age, sex, tumor stage and tumor diameter between the two groups(all P>0.05). The lymph node metastasis rate in A group was 17.9%(7/39), which was higher than that in B group[6.1%(23/379)](χ2=5.814, P=0.016). The incidence rate of temporary hypoparathyroidism in A group was 28.2%(11/39), which was higher than that in B group[13.2%(50/379)](χ2=6.394, P<0.05). The local recurrence rate in A group was 5.1%(2/39), which was higher than that in B group[2.4%(9/379)], but there was no statistically significant difference(χ2=0.248, P=0.619). The 5-year disease-free survival rate in A group was 84.6%(33/39), which was lower than that in B group [95.8%(363/379)](χ2=6.740, P<0.05). Conclusion Hyperthyroidism complicated with thyroid carcinoma has high aggressive behavior and should be treated actively. Key words: Hyperthyroidism; Thyroid neoplasms; Thyroid function tests; Hypothyroidism; Neoplasm staging; Lymphatic metastasis; Neoplasm recurrence, local; Survival rate; Pathology, clinical