Background: The healing of chronically infected wounds is severely hindered by persistent inflammation, bacterial infection, and oxidative stress, posing great challenges to clinical therapy. To address these challenges, we designed a multifunctional dual-layer microneedles patch (MN@DOX+RES) featuring reactive oxygen species (ROS) responsiveness and dual drug delivery capabilities. This patch is engineered to deliver synergistic antibacterial, anti-inflammatory, and antioxidant effects, thereby promoting the healing of infected wounds. Methods: The dual-layer microneedles patch comprises a rapidly dissolvable HA backing layer loaded with DOX and a ROS-responsive tips layer composed of a crosslinked AHA-PBA/PVA matrix that encapsulates water-soluble RES inclusion complexes. A series of in vitro experiments was conducted to evaluate the mechanical strength, biocompatibility, antibacterial activity against Staphylococcus aureus and Escherichia coli, antioxidant performance, and macrophage polarization. In vivo evaluations were performed on rat models with infected skin wounds. Results: The MN@DOX+RES microneedles exhibited strong skin penetration ability and excellent mechanical strength. It significantly inhibited bacterial growth, efficiently scavenged free radicals, reduced intracellular ROS levels, and enhanced M2 macrophage polarization. In vivo, the patch accelerated wound closure, suppressed the inflammatory cytokine IL-6, enhanced IL-10 expression, and activated the Keap1/Nrf2/HO-1 antioxidant signaling pathway. Conclusions: This study proposes an innovative therapeutic strategy that combines dual-drug delivery, oxidative microenvironment regulation, and immune modulation to promote the healing of chronic infected wounds. The MN@DOX+RES microneedles system demonstrates great potential in overcoming clinical challenges associated with infection, inflammation, and the limitations of conventional therapeutic approaches.

Piezoelectric and bactericidal effects of GaSe-loaded poly-L-lactic acid fibrous dressings under ultrasound stimulation for improved MRSA-infected wound healing.

Promotes MRSA infected wound healing by using photothermal responsive and ROS generating black phosphorus nano colloids enhanced hydrogel.

Mussel-inspired adhesive macromolecule modified zinc oxide nanoparticles incorporated into polyethylene glycol hydrogels for enhancing infected wound healing.

Silk fibroin/poly(L-lactic acid) Janus nanofibre membranes enhanced with Al-Ti₃C₂Tₓ MXene for dual-modal conductive and NIR-photothermal synergistic therapy of infected diabetic wounds.

Antibiotic resistance characteristics and genetic determinants of hypervirulence in Klebsiella pneumoniae isolated from wounded individuals in Ukraine.

Risk Factors and Consequences of Bleeding Complications after Transcarotid Artery Revascularization in the Vascular Quality Initiative.

Various dressings have been developed for the prevention and treatment of wound infections, but the complex structures and manufacturing processes designed to achieve powerful functionalities have impeded their clinical application. Herein, a dynamic injectable photothermal/chemotherapeutic hydrogel has been facilely established through mixing gellan gum (GG), indocyanine green (ICG) and amikacin (AMI) aqueous solutions at 80°C and cooling to room temperature. The hydrogel displayed a precise structure with 1.5% of GG containing ICG content of 100μg/mL and AMI content of 1mg/mL, and exhibited favorable injectable, self-healing, and adhesive capabilities as well as superior swelling and moisturizing properties through GG's features. Furthermore, the GG also endowed the hydrogel with the capability to efficiently release drugs in response to the microenvironment (pH 5.0, 7.4 and 8.0) of both infected and uninfected wounds. These exceptional physicochemical properties and combined effects of chemotherapy and PTT facilitated the satisfactory in vitro biocompatibility and antibacterial capability as well as wound healing acceleration ability. Therefore, such a dynamic injectable photothermal/chemotherapeutic hydrogel paves the way toward easily clinical transformation for prevention and treatment of local wound infections.

Surgical treatment of metastatic epidural spinal cord compression: a systematic review and meta-analysis.

Copper-based nanozyme-doped fiber-catalytic membranes mediate reactive oxygen species and tissue regeneration-promoting functions against marine Vibrio vulnificus infections.

Surface-engineered MnO₂@Au@ZIF-67 hierarchical catalysts for acid-triggered antibacterial therapy and infected wound healing.

Targeting ferroptosis and oxidative stress: Ipomoea pes-caprae-synthesized selenium nanoparticles accelerate healing in infected wounds via Nrf2/HO-1 activation

Glucose-activated cascade nanozyme hydrogels for synergistic antibacterial therapy via cascade reaction and microenvironment modulation in diabetic wounds.

Mechanically adaptive injectable hydrogels with dual-role oxidized cyclodextrin for pH-responsive drug delivery in chronic infected wound healing.

Light-activated chitosan hydrogel: A bioadhesive platform for emergency hemostasis and bacteria-infected wound management.

Single-cell-guided design of glycyrrhizic acid hydrogel for metabolic reprogramming and healing of infected diabetic wounds

A poly(lipoic acid)-based elastomer adhesive with synergistic activity of microenvironment regulation and peripheral neuropathy repair facilitates infectious diabetic wound healing.

Self-pumping Janus nanofiber membrane with pH monitoring capability, integrated with a drug-loaded fast-dissolving layer for enhanced chronic wound healing.

Multi-functional poly(N-isopropylacrylamide)-based hydrogel with hemostasis, antibacterial, and sustained drug release properties for infected wound treatment.

Bacterial biofilms are a major cause of diabetic chronic wound healing failure, with effective solutions still challenging. This study prepares core-shell Cu-NiS2@COF@PEG nanoreactors with dual enzyme-like/photothermal effects. In vitro and in vivo studies confirmed that they have multi-mode sterilization, promote collagen deposition, and accelerate angiogenesis to promote healing.