Hyaluronic acid-based microneedles: materials design, fabrication strategies, challenges, and solutions for biomedical applications.

Functionalized smart surfaces to control the wound healing after glaucoma's surgery.

Body temperature-responsive Janus patches accelerate diabetic wound regeneration via coupling mechanical transduction and biochemical pathways.

LncRNA SNHG1 promotes epithelial-mesenchymal transition and progression in clear cell renal cell carcinoma via the miR-200a/ZEB1 axis.

Mitochondria-targeted redox-logic carbon dots for in vivo realtime visualization of peroxynitrite during wound-healing.

Likelihood-free parameter inference for spatiotemporal stochastic biological models using neural posterior estimation.

Scar-free wound healing: Advances in biomaterial-based formulations, smart dressings and bioactive therapeutics - a comprehensive review.

Lipoteichoic acid as a key wound healing mediator in Gram-positive bacteria.

Chronic lower-extremity ulcers remain a major therapeutic challenge. Conventional treatments-including compression therapy, debridement, dressings, antibiotics, pharmacologic agents, hyperbaric oxygen, and skin grafting-frequently fail to provide durable closure, largely due to limited modulation of the wound-healing cascade. Autologous platelet-rich plasma (PRP) is gaining interest as a regenerative adjunct capable of delivering concentrated endogenous growth factors. We report a 58-year-old woman with more than 20 previous skin grafting procedures for recurrent lower-leg ulcers, arising from self-inflicted trauma and secondary infections. Multidisciplinary care of a 15 cm × 18 cm ulcer incorporating debridement, targeted antibiotics, and mainly PRP, administered in six sessions, contributed substantially to ulcer reduction and finally to complete wound healing. PRP has shown promise in enhancing wound closure rates, healing time, and tissue quality. However, the absence of standardized protocols for preparation, dosing, and application, as well as inconsistent outcome reporting, limits its broader adoption. This case underscores the potential role of PRP as a central component in managing complex, refractory ulcers, particularly when behavioural and infectious factors impede healing. Standardized protocols and robust clinical trials are warranted to clarify its role in chronic wound care.

Untargeted LC-MS/MS profiling of Prunus korshinskyi leaf and bark based on UPLC-QTOF-MS analysis with evaluation of wound healing potential.

The optimal method of wound closure after ileostomy reversal remains uncertain. Purse-string approximation reduces surgical site infections but requires prolonged wound care. Combining negative pressure wound therapy with primary linear closure may offer a more convenient approach while maintaining acceptable infection rates. To determine whether primary linear closure with negative pressure wound therapy is noninferior to purse-string approximation in preventing surgical site infection after ileostomy reversal. Prospective, nonblinded, multi-institutional, noninferiority randomized controlled trial. Community tertiary hospital and academic medical center. Adults undergoing elective ileostomy reversal between October 2018 and March 2024. Participants were randomly assigned to undergo either primary linear closure with negative pressure wound therapy or purse-string approximation for skin closure after ileostomy reversal. The primary outcome was the occurrence of surgical site infection, with a noninferiority margin set at 16%. Secondary outcomes included time to wound healing and scar appearance using the validated Patient and Observer Scar Assessment Scale. One hundred twelve patients completed the study, with 61 in the negative pressure wound therapy arm and 51 in the purse-string approximation arm. Primary linear closure negative pressure wound therapy was noninferior to the purse-string approximation arm in terms of surgical site infection: 7% and 2%, respectively, with an absolute risk difference of 5% (95% CI, 2.5%-12.5%). Early wound healing at 2 weeks was achieved in 77% vs 23.5% of patients, respectively ( p < 0.001, generalized estimating equation analysis). All wounds healed by 6 weeks in both groups. No negative pressure wound therapy device malfunctions occurred. Nonblinded study limited to 2 institutions. Ileostomy reversal by primary linear closure negative pressure wound therapy is noninferior to purse-string approximation in terms of the occurrence of surgical site infections while achieving significantly faster wound healing. The method is safe, convenient, and cosmetically acceptable. See Video Abstract . Advocate Aurora Health IRB, IRB# 22.034 (7082). ANTECEDENTES:El método óptimo para el cierre de heridas tras la reversión de una ileostomía sigue siendo incierto. La aproximación en jareta reduce las infecciones del sitio quirúrgico, pero requiere un cuidado prolongado de la herida. La combinación de la terapia de presión negativa para heridas con el cierre lineal primario podría ofrecer un enfoque más conveniente, manteniendo al mismo tiempo tasas de infección aceptables.OBJETIVO:Determinar si el cierre lineal primario con terapia de presión negativa para heridas es no inferior a la aproximación en jareta en la prevención de infecciones del sitio quirúrgico tras la reversión de una ileostomía.DISEÑO:Ensayo controlado aleatorizado, prospectivo, no enmascarado, multiinstitucional y de no inferioridad.ESCENARIOS:Un hospital terciario comunitario y un centro médico académico.PACIENTES:Adultos sometidos a una reversión electiva de ileostomía entre octubre de 2018 y marzo de 2024.INTERVENCIÓN:Los participantes fueron aleatorizados para recibir, como cierre cutáneo tras la reversión de la ileostomía, bien un cierre lineal primario con terapia de presión negativa para heridas, bien una aproximación en jareta.MEDIDAS DE RESULTADO PRINCIPALES:El resultado primario fue la aparición de infecciones del sitio quirúrgico, con un margen de no inferioridad establecido en el 16%. Los resultados secundarios incluyeron el tiempo hasta la cicatrización de la herida y el aspecto de la cicatriz, evaluados mediante la Escala de Evaluación de Cicatrices por el Paciente y el Observador (Patient and Observer Scar Assessment Scale), la cual está validada.RESULTADOS:Ciento doce pacientes completaron el estudio: 61 en el brazo de terapia de presión negativa para heridas y 51 en el brazo de aproximación en jareta. El cierre lineal primario con terapia de presión negativa para heridas resultó no inferior a la aproximación en jareta en términos de infecciones del sitio quirúrgico (ISQ): 7% y 2%, respectivamente, con una diferencia de riesgo absoluta del 5% (IC del 95%: -2,5% a 12,5%). Se logró una cicatrización temprana de la herida a las 2 semanas en el 77% de los pacientes del primer grupo frente al 23,5% del segundo (p < 0,001; análisis GEE). Todas las heridas habían cicatrizado a las 6 semanas en ambos grupos. No se produjeron fallos de funcionamiento en los dispositivos de terapia de presión negativa para heridas.LIMITACIONES:Estudio no enmascarado, limitado a dos instituciones.CONCLUSIONES:La reversión de la ileostomía mediante cierre lineal primario con terapia de presión negativa para heridas es no inferior a la aproximación en jareta en términos de la aparición de infecciones del sitio quirúrgico, logrando al mismo tiempo una cicatrización de la herida significativamente más rápida. Este método es seguro, conveniente y estéticamente aceptable. (AI-generated translation )Registro del ensayo:IRB de Advocate Aurora Health, N.º de IRB 22.034 (7082).

Alginate-based composite hydrogel derived from Azotobacter vinelandii Av1: Preparation, properties, and wound-healing efficacy.

Impaired wound healing in patients with diabetes mellitus is a major clinical problem resulting from chronic hyperglycemia, which disrupts immune function and prolongs inflammation. Cell-based therapies such as mesenchymal stem cells (MSCs) and stromal vascular fraction (SVF) have shown promise in promoting tissue repair, yet comparative data between SVF and adipose-derived stem cells (ADSCs) remain limited. In this experimental study, a diabetic rat model was used to compare the effects of allogeneic SVF and allogeneic ADSCs on wound healing. Wound areas were measured on days 5, 10, and 14. Histological analyses, including assessments of reepithelialization, fibrosis, and epithelial tongue length, as well as cytokine level measurements, were performed to evaluate tissue regeneration and inflammation. Both SVF and ADSC groups demonstrated significantly smaller wound areas compared with controls at all time points ( P <0.05). On days 5, 10, and 14, the wound areas were 3.127±0.382, 0.833±0.360, and 0.552±0.185cm 2 in the control group; 1.968±0.533, 0.543±0.165, and 0.347±0.144cm 2 in the SVF group; and 2.242±0.565, 0.432±0.137, and 0.276±0.131cm 2 in the ADSC group, respectively. Histological evaluation revealed enhanced reepithelialization and greater epithelial tongue length in both treatment groups. No significant differences were observed between SVF and ADSC groups. IL-12 levels on day 5 decreased in the SVF group (76.01±13.65ng/L) and increased in the ADSC group (96.50±19.66ng/L) compared with the control group (84.81±15.03ng/L) ( P =0.047). Both allogeneic SVF and ADSC therapies significantly accelerated wound healing in diabetic rats. Given its simpler and faster isolation process, lower cost, and comparable efficacy to ADSCs, SVF may represent a practical and effective alternative for enhancing diabetic wound repair.

Evidence synthesis in pilonidal disease surgery is limited by heterogeneity in outcome selection, definition, measurement and follow-up. This systematic review aimed to identify and describe outcomes reported in comparative studies of surgical management of pilonidal disease to inform development of a core outcome set. PubMed, Embase and CENTRAL were searched from inception to 28 November 2025. Randomised controlled trials and comparative observational studies evaluating surgical interventions for pilonidal disease were eligible. Two reviewers independently screened studies and extracted study characteristics and outcome data. Verbatim outcomes were standardised, grouped into domains and summarised descriptively. Because the objective was to map outcome reporting rather than estimate treatment effects, risk-of-bias assessment and GRADE appraisal were not undertaken. A total of 155 studies were included, comprising 93 randomised trials and 62 observational studies. Ten outcome domains were identified. Recurrence was reported most frequently (112 studies, 72.3%), followed by non-infective complications (103, 66.5%), pain (83, 53.5%), wound healing (82, 52.9%) and return to normal activities (63, 40.6%). Patient-important domains were less frequently reported, including health-related quality of life or satisfaction (50, 32.3%) and cosmesis (17, 11.0%). Among outcomes designated as primary, wound healing was most common (51 studies, 32.9%), followed by recurrence (35, 22.6%) and postoperative complications (25, 16.1%). Pain (6, 3.9%), return to normal activities/work (9, 5.8%), and psychological or quality-of-life outcomes (3, 1.9%) were rarely primary outcomes. Definitions, measurement instruments, assessment timepoints and overall follow-up duration varied substantially across studies. Comparative studies of pilonidal disease surgery predominantly prioritise recurrence, healing and complications, whereas patient-important outcomes are reported less consistently and are infrequently prioritised. Standardisation of outcome selection, definitions and measurement is needed. These findings support development of a core outcome set for future trials in pilonidal disease surgery. CRD420251128698.

Injectable photo-crosslinked hyaluronic acid/carboxymethyl cellulose hydrogel for the sustained delivery of rhFGF2 and accelerated wound healing.

Outcomes among hemodialysis-dependent patients undergoing infrapopliteal revascularization for chronic limb-threatening ischemia.

Scaffold-mediated miRNA-155 inhibition promotes regenerative macrophage polarisation leading to anti-inflammatory, angiogenic and neurogenic responses for wound healing.

Umbilical Inset Incision Type Influences Abdominal Donor Site Healing in Autologous Breast Reconstruction.

Mannose-decorated N-succinyl chitosan nanoparticle film: A novel approach for enhanced wound healing.

This study aimed to investigate the mechanisms underlying impaired oral wound healing in aged individuals, with a focus on the roles of neutrophil extracellular traps (NETs) and NLRP3 inflammasome activation. Palatal wound healing was compared between young and aged mice. We assessed the change of neutrophil infiltration, NETs formation, and NLRP3 inflammasome activation in mucosa using immunohistochemistry, RNA sequencing, and flow cytometry. Invitro co-culture assays evaluated NETs' effects on fibroblast function and NLRP3 inflammasome activation. The therapeutic potential of NETs-NLRP3 clearance was tested invivo with DNase1 and MCC950. To validate clinical relevance, human single-cell RNA sequencing data were further analyzed. Aged mice exhibited delayed oral wound healing, characterized by increased neutrophil recruitment and a heightened propensity for fibrin-induced NETs formation. NETs activated the NLRP3 inflammasome in mucosal fibroblasts, suppressing their proliferation and migration. Notably, targeting NETs-NLRP3 inflammasome reduced inflammation and accelerated wound closure in the aged group. Corresponding human data further revealed increased NETs-associated neutrophil subsets and enriched NLRP3 pathways in aged oral mucosa. Our findings demonstrate that enhanced NETs formation and subsequent NLRP3 inflammasome impair wound healing in aged oral mucosa. Targeting NETs-NLRP3 thus represents a promising therapeutic strategy to improve mucosal repair.