The properties of Ocicmum gratissimum aqueous extract against ultraviolet-C-induced inflammation.

Exploring traditional Japanese herbal ointments for wound healing: A wide diversity of practices, metabolites, and effects.

Introduction: Wound healing following abdominal surgeries such as elective Lower Segment Caesarean Section (LSCS) is a multifactorial process influenced by physiological and psychological factors. Elevated psychological stress adversely affects immune system performance, which can lead to slower tissue regeneration and heightened susceptibility to postoperative complications. Meditation grounded in yoga principles, characterised by controlled breathing and mindful awareness, has been documented to lower stress and inflammation while supporting autonomic nervous system stability. Despite these potential benefits, there is limited clinical evidence evaluating the direct role of yoga-based meditation in improving surgical wound healing. Aim: To evaluate the impact of yoga-based meditation on stress levels and wound healing outcomes in patients after elective LSCS. Materials and Methods: A randomised controlled trial was conducted in July 2022 at the Department of Obstetrics and Gynaecology, Shri B.M Patil Medical College, Hospital and Research Centre, Vijayapura, Karnataka, India. The study included 52 patients undergoing elective LSCS, who were randomly assigned to two groups: an intervention group and a control group. Participants were recruited at the time of admission to the postnatal ward. The intervention group received guided yoga-based meditation for 15 minutes, three times daily, over a period of 14 consecutive days. Meditation sessions focused on breath awareness, relaxation techniques, and mindfulness. The control group received standard postoperative care without meditation. Stress parameters and wound healing outcomes were assessed at baseline and on day 14. The parameters measured included Heart Rate (HR), Heart Rate Variability (HRV), Perceived Stress Score (PSS), and Galvanic Skin Response (GSR). Wound healing was assessed using the Southampton wound grading system. Statistical analysis was performed using International Business Machine (IBM) Statistical Packages of Social Sciences (SPSS) Statistics version 25.0. Between-group comparisons were made using the chi-square test for categorical variables and the Mann-Whitney U test for non parametric continuous variables. A p-value of <0.05 was considered statistically significant. Results: Of the 70 patients enrolled, 52 completed the trial (26 per group). There were no significant baseline differences in age, Body Mass Index (BMI) or parity (p>0.05). After 14 days, the yoga-meditation group showed a greater reduction in resting HR (mean change -14.48% vs -4.02%; p<0.001), a greater increase in HRV (mean change +21.81% vs +8.75%; p<0.001), and a greater reduction in PSS score (-48.2% vs -47.4%; p=0.013) compared with controls. GSR showed a marked increase, indicating enhanced parasympathetic activity. Although both groups achieved 100% Grade 0 healing by day 14 (Southampton Wound Grading System), the meditation group demonstrated a more rapid resolution of early inflammatory signs in the first week. Conclusion: Adjunctive yoga-based meditation significantly attenuates postoperative stress- both physiological and psychological and may accelerate the initial phases of wound healing following elective LSCS. This non invasive, low-cost intervention holds promise for enhancing recovery and patient well-being in the postoperative setting.

Adipose-derived stem cells (ADSCs)-derived DKK1 promotes cutaneous wound healing by inducing M2 macrophage polarization via PI3K/AKT and JNK activation.

The objective of this study was to evaluate the efficacy of scraping without endothelial keratoplasty technique using a novel cell scraper for treating corneal endothelial dysfunction and explore the adjuvant effects of a ROCK inhibitor (ROCKi). Human donor tissues (n = 3) were mounted on an artificial anterior chamber, and one-half of the corneal endothelium was scraped with a novel cell scraper. The tissues were then stained with hematoxylin and eosin (H&E) to assess cell removal. In parallel, central peeling (descemetorhexis) using sharp acute forceps (n = 8) and cell scraping using a novel cell scraper (n = 8) was compared with and without ROCKi to investigate the wound healing response. RT-PCR analysis to assess the endothelial-to-mesenchymal transition using ZEB1 , SNAI1/2 , VIM , and CDH1/2 genes was performed after storage. An ex vivo surgical setup using an artificial anterior chamber demonstrated the feasibility and efficacy of using the cell scraper. Superior wound healing (88%) was observed in the endothelial cell-scraping group compared with Descemet membrane peeling (22%) at day 16. Application of ROCKi expedited wound healing after endothelial scraping (97%) and peeling (40%) compared with their respective controls at day 16. ROCKi treatment promoted endothelial-to-mesenchymal transition; however, with enhanced wound healing after cell scraping, endothelial cells formed a monolayer on native Descemet membrane compared with the peeling method. Scraping without endothelial keratoplasty can be performed effectively using the cell scraper and offers a potential alternative to endothelial keratoplasty, which can be effective in treating bullous keratopathy with healthy peripheral endothelial cells. ROCKi enhances wound healing, supporting further clinical testing and potentially delaying corneal tissue implantation.

Dual-responsive SERS nanoprobe for real-time imaging of pH and ROS dynamics in wound healing.

USP7 inhibition promotes wound healing by suppressing M1 macrophage polarization via NF-κB/MAPK signaling pathway.

Antibacterial chitosan-gelatin-PEG incorporated with ZiF-8 hydrogels as bioactive wound dressing for wound healing application.

Histamine induces vascular endothelial cell proliferation via the histamine H1 receptor-extracellular regulated protein kinase 1/2-cyclin D1/cyclin-dependent kinase 4/6 axis.

Anti-inflammatory activity of 7-methoxycoumarin isolated from Ayapana triplinervis Vahl (Compositae) via inhibition of inflammatory mediators - In-vivo, in-vitro and in-silico studies.

Therapeutic efficacy and concomitant toxicity of Hongsheng Dan in enhancing healing of chronic refractory wounds in rats.

Epithelial-mesenchymal transition (EMT) and cell migration are two essential cellular processes involved in normal biological events such as embryogenesis, organ development, and wound healing, and are also associated with pathological conditions like cancer metastasis. Recent studies have indicated that the microtubule cytoskeleton and its associated proteins play significant roles in these processes. In this study, we investigated how fidgetin, a microtubule-severing and depolymerizing enzyme, affects EMT and cell migration by depleting it in MDA-MB-231 breast cancer cells. Our data show that depletion of endogenous fidgetin reduces the cell migration rate in both wound-healing and single-cell motility assays. During EMT, transcription factors such as Snail, Slug (Snail2), Twist, and Zeb play pivotal roles by regulating the expression of EMT-related genes. In this study, we found that fidgetin depletion reduces the expression of Slug and Zeb1 in MDA-MB-231 breast cancer cells under both basal and EMT-induced conditions. Consistent with these findings, we observed that fidgetin depletion downregulates N-cadherin and vimentin expression in EMT-induced MDA-MB-231 cells, thereby influencing cell motility. Further investigations revealed that fidgetin also affects microtubule plus-end tracking proteins (+TIPs). Specifically, we detected reduced expression of CLIP-170 in fidgetin-depleted cells. Immunofluorescence analysis showed that EB1 comets occupied a smaller area at microtubule plus ends upon fidgetin depletion. Additionally, the size of focal adhesions was significantly increased, although no changes were observed in the expression levels of focal adhesion kinase (FAK). Our findings indicate that microtubule regulation by fidgetin influences cancer cell motility by altering the expression of EMT-promoting transcription factors and modulating the accumulation of focal adhesion and EB1 proteins. These results suggest that fidgetin could be a promising therapeutic target in cancer.

Self-pumping Janus nanofiber membrane with pH monitoring capability, integrated with a drug-loaded fast-dissolving layer for enhanced chronic wound healing.

Introduction: Gingival recession causes root exposure, aesthetic concerns, and hypersensitivity. Although surgical techniques can achieve root coverage, adjunctive root surface biomodifiers, such as citric acid, Ethylenediaminetetraacetic Acid (EDTA), and platelet-rich derivatives, aim to enhance healing and attachment. Their clinical efficacy, however, remains a matter of debate. Aim: To evaluate the effectiveness of wound healing agents as root biomodifiers in improving clinical outcomes and patient satisfaction in the management of gingival recession. Materials and Methods: This review followed PRISMA guidelines and was registered with PROSPERO. A comprehensive search across PubMed, Scopus, Web of Science, and Cochrane databases identified clinical trials evaluating Root Surface Biomodification (RSB) agents in managing gingival recession. Eligible studies included surgical interventions with or without RSB, assessing outcomes like root coverage, gingival thickness, and patient-reported satisfaction. Data were narratively synthesised due to study heterogeneity. Only clinical trials involving human participants with gingival recession defects were included, with randomised controlled and split-mouth designs being the most common. The primary outcomes were root coverage percentage, gingival thickness, and Clinical Attachment Level (CAL), while the secondary outcomes included patient-reported satisfaction, aesthetic outcomes, wound healing indices, and postoperative discomfort. Control groups were consistently represented by conventional mucogingival surgical techniques performed without the use of adjunctive RSB. The efficacy of wound healing agents was primarily evaluated in terms of root coverage gain, improvement in clinical attachment, and increase in gingival thickness, with additional assessments of early wound healing and patient-reported benefits. Considerable heterogeneity in methodology was evident across studies, arising from variations in biomodification protocols, surgical techniques, follow-up duration, and outcome assessment methods, which limited comparability and precluded meta-analysis. Results: Eight studies were included, evaluating various root surface biomodifiers like Platelet-rich Fibrin (PRF), Titaniumprepared PRF (TPRF/T-PRF), citric acid, and EDTA. PRF-based interventions improved early healing and patient satisfaction but showed limited long-term advantages. Chemical agents and laser-assisted techniques demonstrated inconsistent benefits. The evidence quality ranged from moderate to high, with limitations in standardisation and generalisability. Conclusion: Conventional mucogingival surgical techniques are highly effective for root coverage, while the long-term benefits of RSB agents remain inconclusive. RSB may offer adjunctive benefits but lacks sufficient evidence for routine application in clinical practice.

Background: The pathological hyperglycemic microenvironment in diabetic wounds increases susceptibility to bacterial infections and impairs wound healing. However, despite certain advancements in conventional clinical treatments, the pathological issues have not yet been fundamentally resolved. The mechanism that amplifies ferroptosis through disruption of the bacterial electron transport chain (ETC) results in effective bacterial eradication and facilitates wound healing, thereby offering novel therapeutic potential for the management of diabetic wound infections. Methods: This work designs a multi-enzyme-mimicking Fe-WS2@GOx nanozymes by loading glucose oxidase (GOx) onto defect-engineered Fe-WS2, which disrupts the bacterial ETC and induces ferroptosis, thereby accelerating diabetic wound healing. Results: During the bacterial infection stage, the Fe-WS2@GOx nanozymes with abundant sulfur vacancies can simultaneously mitigate hyperglycemic and alleviate the hypoxic microenvironment. This is achieved through continuously producing substantial amounts of reactive oxygen species (ROS), resulting in endogenous glucose consumption, promoting cyclic accumulation of H2O2, and ensuring a sustained oxygen supply. Meanwhile, the generated ROS interferes with the bacterial ETC, impedes bacterial energy metabolism and inhibits biosynthesis, ultimately leading to bacterial death. More importantly, at the new tissue proliferation stage, Fe-WS2@GOx can promote wound angiogenesis and tissue regeneration by macrophage immunomodulatory effect. Conclusions: Therefore, this study provides a new paradigm strategy for diabetic wound infections therapy by "electron transport chain interference" amplified bacterial ferroptosis.

Introduction: Surgical Site Infections (SSIs) pose a significant challenge in various surgical procedures, including periodontal surgery. Sutures used in the oral cavity are constantly exposed to saliva, which creates a wicking effect that can impede the healing process after surgery. To address these issues, modifications to sutures are necessary. Need for the study: Coated sutures that provide local, sustained release of antibiotics may effectively prevent biofilm formation and reduce bacterial counts in the periodontal surgical area. These innovative sutures combine two drugs, which can help mitigate the wicking effect, promote faster healing, and provide effective antimicrobial action. To date, no clinical studies have evaluated such a combination in suture form, prompting the design of this animal study. Currently, triclosan-coated sutures are the only commercially available option, and the new combination sutures could serve as an alternative. There is a lack of comparative data regarding the antimicrobial and wound-healing potential of alternative antibiotic combinations, particularly in the context of periodontal surgery. Aim: To evaluate and compare the efficacy of amoxicillinand metronidazole-coated sutures versus triclosan-coated sutures in terms of antimicrobial activity and wound-healing properties. Materials and Methods: This in vitro experimental study on rabbits will be conducted in the Department of Pharmacology at the Central Preclinical Research Facility, Datta Meghe College of Pharmacy, Sawangi (Meghe), Wardha, India, from April 2025 to December 2025. The study will be carried out in two phases. In Phase I, amoxicillin-and metronidazole-coated sutures will be prepared and their drug release evaluated. In Phase II, an animal study will be conducted. The study will include 12 New Zealand rabbits of both sexes, with an average weight of 2-4 kg. The rabbits will be categorised into two interventional groups, with six rabbits in each group. Rabbits in Group I will receive 4-0 sutures coated with the combination of amoxicillin and metronidazole in the interdental area after flap elevation. In Group II, 4-0 sutures coated with triclosan will be used to assess antimicrobial activity and wound healing. For inter and intragroup comparisons, unpaired and paired t-tests will be applied, respectively. A p-value of <0.05 will be considered statistically significant.

A poly(lipoic acid)-based elastomer adhesive with synergistic activity of microenvironment regulation and peripheral neuropathy repair facilitates infectious diabetic wound healing.

Effects of Brazilian green propolis and artepillin C on collagen metabolism and fibroblast behaviors: Implications for skin wound healing.

Glucose-activated cascade nanozyme hydrogels for synergistic antibacterial therapy via cascade reaction and microenvironment modulation in diabetic wounds.

Thiol-ene photoclick hydrogels reinforced with poly(protocatechualdehyde)-coated gallium doped bioactive glass nanoparticles for scarless healing of infected wound.