Wound Healing Effect Research Articles

Oxygen-Supplying Containers to Fabricate Immunomodulatory Hydrogels for Expediting Wound Healing via Acute Oxidative Stress.

Chronic wounds impose severe health and economic burdens due to persistent inflammation and complications. Immunomodulatory hydrogels have emerged as promising therapies that modulate inflammatory responses for wound healing. Oxygen plays a crucial role in immune modulation and tissue regeneration; however, current oxygen-delivering biomaterials are limited by the mandatory incorporation of oxygen-generating agents and cytotoxic byproducts. Herein, we develop an oxygen-supplying container (Oxygener) via dopamine-mediated catalase immobilization to create oxygen-delivering and immunomodulatory hydrogels. Oxygener supplies oxygen in various solutions by hydrogen peroxide decomposition, producing cytocompatible, oxygen-enriched media for fabricating implantable oxygen-delivering hydrogels. These oxygenated systems facilitate the transition from pro-inflammatory to anti-inflammatory states by reducing macrophage recruitment while improving cellular proliferation and tissue remodeling at the gene and tissue levels in vivo. Our findings establish the minimum oxygen tension and supply duration required for effective wound healing. In summary, Oxygener holds great potential for developing various oxygen-delivering formulations for advanced wound care.

Development of the composition and characteristics of a wound-healing gel with L-arginine

Background. Currently, the issues of skin and mucous membrane wound therapy are becoming increasingly clear. One of the most modern and popular dosage forms of wound healing agents is a gel, which has undeniable pharmacological and technological advantages. L-arginine, which is a substrate for nitric oxide (II) (NO (II) synthases), which are involved in the formation of NO (II), was used as the main active ingredient in the developed dosage form.Aim. Selection of the optimal gelling agent and development of the composition of a soft dosage form used for healing wounds of the skin and mucous membranes.Materials and methods. Three model samples of gels made using potentially promising gelling agents were selected as objects of study: carbopol 940, sodium carboxymethyl cellulose, methyl cellulose. Rheological characteristics of the samples were studied by rotational viscometry on a Fungilab Premium viscometer (Spain) using a “cylinder in cylinder” measuring system. To study the biodegradation of the model sample, a FADT-1202A dissolution tester (FOCS Co., Ltd, China) equipped with a paddle stirrer was used. The degree of arginine release from the samples was estimated by the Kruvchinsky equilibrium dialysis method.Results. As a result of studying the structural and mechanical properties of the model samples, it was revealed that methylcellulose in combination with L-arginine allows creating a gel composition with the greatest stability. when studying the biodegradation of the methylcellulose-based composition, it was found that the gel is capable of being on the oral mucosa for no more than 60 minutes. Then, using the Kruvchinsky equilibrium dialysis method during this period of time, the nature of the L-arginine release from the developed composition was established.Conclusion. The conducted studies substantiated the choice of the wound-healing gel base. Based on the obtained results, methylcellulose was chosen as the optimal gelling agent for the production of a soft medicinal form with a wound-healing effect.

Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses

Lactoferrin (LF), a multifunctional glycoprotein found abundantly in bovine colostrum, is known for its regenerative and anti-inflammatory properties. In this study, we investigated the wound healing and immunomodulatory effects of colostrum-derived exosome-encapsulated lactoferrin (EV-exoLF) on dermal fibroblasts. EV-exoLF was isolated and characterized via nanoparticle tracking analysis and flow cytometry. Functional assays demonstrated that EV-exoLF significantly promoted fibroblast viability and migration in both mouse NIH/3T3 and human HS-68 cell lines. Furthermore, EV-exoLF reduced the expression of pro-inflammatory cytokines (IL-1 and IL-6) and phosphorylated JNK in lipopolysaccharide (LPS)-treated fibroblasts. These findings suggest that EV-exoLF not only enhances fibroblast-mediated wound closure but also mitigates inflammation, highlighting its therapeutic potential in skin regeneration. Colostrum-derived exosomal lactoferrin may serve as a promising natural, cell-free strategy for managing inflammatory skin conditions and improving wound healing outcomes.

Phytocomponents screening for oncoprotector development

Background. Medicinal plants have a high chemopreventive potential in oncology. The structural diversity of plant biologically active substances: phenolic compounds (simple phenols, flavonoids, phenolic acids, tannins, etc.), terpenoids (mono-, diterpenoids, triterpene saponins, etc.), essential oils, for example, results in a wide range of antitumor activity. Complex herbal preparations are more effective and safe due to their multi-target effects on various regulatory mechanisms in combination with relatively low toxicity. The development of effective natural pharmaceutical compositions based on the structural diversity of biologically active substances seems relevant.Aim. Number plant extracts antiproliferative effect against CaOv human ovarian adenocarcinoma cells investigation and potential phytocomponents selection for the novel antitumor pharmaceutical composition development.Materials and methods. CaOv human ovarian adenocarcinoma cell line was used as a test model. The antiproliferative effect of plant extracts was measured by the radiometric method based on the 3H-thymidine incorporation into CaOv cells DNA. Half maximal inhibitory concentration (IC50) was calculated using probit analysis. The selection of vegetable oil as an optimal extraction agent was carried out on C57BL/6 male mice based on the wound healing effect.Results. The antiproliferative activity of 27 medical plant extracts was studied. The 14 most effective ones in these conditions were selected based on the IC50. Linseed oil was chosen as an optimal extraction agent considered the wound-healing effect of individual vegetable oils (linseed, sunflower, corn), as well as the corresponding oil extracts of the phytocomposition.Conclusion. The selected phytocomponents can be used as a foundation for an optimal compound development of a new pharmaceutical composition aimed at chemoprevention in cancer care. The choice of linseed vegetable oil as an experimental phytocomposition extractant is justified.

Innovative Hydrogel Formulation Combining Phycocyanin and Probiotic for Enhancing Skin Regeneration and Accelerated Wound Healing: A Preclinical Investigation in Wistar Rats.

Skin repair is a global concern that requires multifunctional wound healing platforms that accelerate healing and shield the wound from external contamination. Phycocyanin (C-PC) is a natural protein that shows favorable characteristics for skin repair. The present study aims to investigate the synergistic wound healing potential of probiotic Pediococcus pentosaceus and C-PC hydrogel formulation. C-PC whey protein nanofibrils (WPINF) were first prepared and evaluated for particle size, % entrapment efficiency, and zeta potential. Selected WPINF was used to prepare several hydrogel formulations which were further evaluated for physical properties and in vitro C-PC release. Formulation with acceptable physical properties and C-PC release was studied for oxidative stress markers, antioxidant biomarkers, and wound healing effect in animal models. WPINF had acceptable particle size (26.59-153.5nm), % EE (65.76-95.98%), and zeta potential (22.3-26.1 mV). The developed hydrogels exhibited neutral pH values (6.97-7.10), optimal spreadability (5.60-7.50cm), and viscosity (123-209Pa). HEC-based hydrogel (F1) showed superior C-PC release which was found to follow Higuchi diffusion. In vivo, hydrogel formulations (with and without probiotic) showed significantly reduced oxidative stress markers, with MDA reduced by 54.76-98.58% and antioxidant biomarkers (GSH and TAC) ensuring successful delivery of therapeutic compounds. Histological analysis showed improved re-epithelialization, reduced inflammation, and well-formed granulation tissue in treated groups, with probiotic-C-PC group demonstrating additional immunomodulatory effects. The study highlights promising potential of C-PC-probiotic hydrogels as a safeand effective biotherapeutics for wound healing, supporting their advancement into further clinical investigations to validate their efficacy in humans.

Priming effect of photobiomodulation of mesenchymal stem cells on extracellular vesicles for regenerative medicine.

Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) (MSCs-derived EVs) have shown promising outcomes in repairing damaged tissues across various disease models. Combining MSCs-derived EVs with advanced technologies could further enhance their use as a viable cell-free therapy in medical applications. Photobiomodulation (PBM) has shown a synergistic effect with EVs in improving tissue regeneration. This pioneer review aimed to evaluate the entire scope available in the current literature on the PBM priming effect on MSCs-derived EVs. Two independent reviewers conducted a literature search in online databases PubMed/MEDLINE, Web of Science, Scopus, EMBASE and LIVIVO. The inclusion criteria were: (1) studies investigating PBM and MSCs-derived EVs and (2) all language and data. Of 213 found articles, 8 (eight) articles were selected for a complete reading. After the complete reading, 6 (six) articles were selected for data extraction. In general, the studies reported no deleterious effects of PBM on EVs. Furthermore, in vitro, PBM increased concentration of EVs, angiogenic factors, anti-apoptotic protein levels and cell survival. In vivo, PBM and EVs demonstrated positive effects in skin wound healing and pulp regeneration. These studies highlighted the promising potential of combining both therapies (PBM and MSCs-derived EVs) as an innovative strategy in regenerative medicine. However, significant challenges remain, including the need for standardization of EVs isolation and characterization methods, as well as the evaluation of laser irradiation parameters across diverse experimental conditions. Additionally, a deeper understanding of the underlying mechanisms of PBM effects on EVs is crucial to optimize this approach and fully harness its therapeutic potential.

Preparation of a controlled release chitosan transdermal patch loaded by ciprofloxacin/zinc oxide nanoparticles for cutaneous wound healing in rat.

Effective wound healing requires materials that modulate inflammation, enhance tissue regeneration, and promote re-epithelialization. In this study, we developed and evaluated the wound healing efficacy of controlled-release chitosan-based transdermal patches loaded with ciprofloxacin and zinc nanoparticles in rats. Full-thickness excisional wounds (2cm diameter) were created on 75 Wistar rats, assigned to five groups: chitosan (Cs), Cs-ciprofloxacin (CsCi), Cs-zinc (CsZn), Cs-ciprofloxacin-zinc (Cs/Zn@Ci), and untreated control. Tissue samples were collected on days 7, 14, and 21 for histopathological, immunohistochemical, and biochemical analysis. The Cs/Zn@Ci group exhibited significantly enhanced wound closure, approximately 1.8-, 1.54-, and 1.19-fold greater than the control at each respective time point (P < 0.05). The Cs/Zn@Ci-treated group showed marked modulation of inflammation, enhanced fibroplasia and angiogenesis, and improved scar tissue formation in the early phase. Long-term treatment resulted in improved collagen alignment, reduced scar formation, and enhanced epithelial regeneration. Gene expression analysis showed significantly elevated CK14 and EGFR levels in the Cs/Zn@Ci group compared to control, with fold increases ranging from 1.1 to 1.6 across time points (P < 0.05). Additionally, collagen and glycosaminoglycan content were markedly higher in the Cs/Zn@Ci groups throughout the healing phases. These findings demonstrate that Cs/Zn@Ci patches act synergistically to enhance healing through antimicrobial activity, immunomodulation, and matrix regeneration. This novel formulation holds strong clinical potential for improving outcomes in cutaneous wound management.

Preparation, characterization, and evaluation of the wound healing activity of Aconiti Lateralis Radix Praeparata polysaccharide nanofiber-based trauma dressing.

Preparation, characterization, and evaluation of the wound healing activity of Aconiti Lateralis Radix Praeparata polysaccharide nanofiber-based trauma dressing.

A comprehensive review of ethnopharmacology, phytochemistry, pharmacological effects, and quality control of Bletilla Striata.

A comprehensive review of ethnopharmacology, phytochemistry, pharmacological effects, and quality control of Bletilla Striata.

Peptide‐Enhanced Bioactive Hydrogel Combined with Photodynamic‐Phage Synergistic Antibacterial Therapy System Accelerates Infected Wound Healing

Abstract Bacterial resistance poses a serious clinical challenge. This study designs a peptide‐enhanced bioactive hydrogel with a photodynamic‐phage synergistic antibacterial effect that is an efficient antibacterial and promotes wound healing. LaFeO3@C3N4 is characterized with XRD, XPS, SEM, and UV–vis, and the photodynamic properties of LaFeO3@C3N4 producing ROS are confirmed by EPR. The peptide QHREDGS‐modified boronate ester hydrogel is characterized by FT‐IR, 1HNMR, SEM, dynamic mechanical analysis, etc. Combined LaFeO3@C3N4 and phage phipa10, the peptide QHREDGS‐modified boronate ester hydrogel shows synergistic antibacterial and wound healing effects in both in vitro and in vivo experiments. Moreover, the molecular mechanism of tissue regeneration is explored by transcriptomic and protein expression analysis, and the results show that the peptide‐enhanced bioactive hydrogel combined with photodynamic‐phage synergistic antibacterial therapy system (QBC@DP‐P‐phi) significantly promoted cell proliferation and migration by regulating key signaling pathways such as PI3K‐Akt, VEGF, and MAPK. Overall, this novel hydrogel is promising for the clinical treatment of infected wounds.

The South Asian Acalypha Species: A Comprehensive Review on Traditional Uses, Phytochemistry and Pharmacological Aspect.

The genus Acalypha (family Euphorbiaceae) has a longstanding role in traditional medicine across South Asia, used to treat conditions such as diabetes, asthma, gastrointestinal disorders, and skin ailments. Its widespread ethnomedicinal use suggests promising pharmacological applications. This review comprehensively examines the origin, habitat, taxonomy, conventional uses, phytochemistry, and pharmacological properties of Acalypha species (AS). Data were compiled from more than 100 peer-reviewed studies retrieved from multiple scientific databases (e.g., PubMed, PubChem, Scopus, ScienceDirect, and others). Phytochemical structures were generated using Chemaxon Marvin Sketch, and data were evaluated to assess chemical diversity and biological activity. Key species such as Acalypha hispida, Acalypha paniculata, Acalypha fruticosa, Acalypha wilkesiana, and Acalypha indica are prevalent in tropical and subtropical regions. These plants contain various bioactive compounds, including alkaloids, flavonoids, tannins, and phenolics, which demonstrate antimicrobial, anti-inflammatory, and wound-healing effects. These findings support their conventional applications in managing a range of health conditions. However, toxicity profiles vary among species, highlighting the need for careful evaluation. The Acalypha genus represents a valuable source of bioactive compounds with significant therapeutic potential. Although traditional use and preclinical evidence are encouraging, limited clinical data and variability in safety profiles necessitate further investigation. Rigorous pharmacological validation and standardization are essential for the safe and effective integration of AS into modern medicine.

PH-Responsive Bilayer Hydrogel with Synergistic Antibacterial/Anti-inflammatory Effect for Infected Wound Healing.

Hydrogels are promising candidates for wound dressings owing to their good biocompatibility, high-water retention, and extracellular matrix-mimicking structure. However, conventional single-layer hydrogels are hard to cope with given the dynamic pH fluctuations (pH 6-8.9) of infected wounds, which exacerbate bacterial colonization and oxidative stress. Here, we engineered a multifunctional bilayer hydrogel (named PPTC) comprising a hydrophobic polydimethylsiloxane (PDMS) top layer and a polyacrylamide (PAM)-based bottom layer integrated with tannic acid (TA) and curcumin-loaded alginate microspheres (Alg@Cur). The PDMS top layer was adopted to prevent external contamination and moisture loss, while the adhesive PAM-TA-Alg@Cur (PTC) bottom layer was designed to achieve a pH-responsive therapeutic synergy. In vitro studies showed that TA exhibited a rapid release (76.66% within 12 h) to suppress bacterial proliferation and inflammation, while Alg@Cur could slowly release curcumin in a pH-dependent manner (90.32% at pH 8.5 vs 38.06% at pH 6, cumulatively), targeting alkaline microenvironments to mitigate oxidative stress. Their synergy demonstrated potent antibacterial activity (≥99.90% inhibition against Staphylococcus aureus and Escherichia coli) and reactive oxygen species scavenging ability (93.89% DPPH elimination). In a rat-infected wound model, the PPTC bilayer hydrogel synergistically accelerated infected wound healing by reducing inflammation, enhancing collagen deposition, and promoting angiogenesis. This work pioneers a pH-driven therapeutic platform for advancing smart dressings for infected wound management.

Effects of peptides derived from active sites of visfatin on wound healing

Severe wounds (e.g., burns) often result in irreversible scarring, leading to cosmetic and functional impairments as well as secondary complications such as reduced skin strength and itching. In the case of chronic wounds and diabetic ulcers, impaired healing capacity is the primary clinical concern, not scarring. These wounds frequently fail to heal, presenting a therapeutic challenge. Despite ongoing research, the development of scarless, cost-effective, and clinically viable therapies for these complex wounds remains a significant challenge. Angiogenesis plays a crucial role in skin wound healing. Visfatin has been known to have angiogenic and wound healing effects. In our previous study, we derived two angiogenic peptides (Vis-1 and Vis-2) from the active site of visfatin. Therefore, this study is aimed to investigate the wound healing potential of these two peptides using a scratch assay system (in vitro) and full-thickness excision wound healing mice (in vivo). Only Vis-1 peptide had a significant wound healing effect in the in vitro assay by promoting proliferation and migration of keratinocytes and dermal fibroblasts, possibly through activation of Wnt/β-Catenin and MAPK signaling pathway. Vis-1 peptide also showed remarkable wound healing effects in the in vivo assay by accelerating wound healing, inducing angiogenesis, promoting neo-epithelium, decreasing granulation tissue, and increasing collagen fiber formation. These results suggest that the Vis-1 peptide has a potent wound healing activity and may contribute as a novel wound healing agent.

Distinct temporal profiles of AMPs and cytokines in pleural fluids from open and closed thoracic surgeries and exploration of synergy with antibiotics and wound healing effects.

Distinct temporal profiles of AMPs and cytokines in pleural fluids from open and closed thoracic surgeries and exploration of synergy with antibiotics and wound healing effects.

Design and Construction of Platinum-Zinc Oxide Grafted Honey-Chitosan Nanofibers Loaded with Hesperidin and Study Its Antibacterial and Wound Healing Effects

Design and Construction of Platinum-Zinc Oxide Grafted Honey-Chitosan Nanofibers Loaded with Hesperidin and Study Its Antibacterial and Wound Healing Effects

Effect and mechanism of non-homopolymeric schizophyllan in wound healing.

Effect and mechanism of non-homopolymeric schizophyllan in wound healing.

Bioinspired nanofiber dressings with counter-transport of exudate and drug for treating heavily exuding wounds.

Bioinspired nanofiber dressings with counter-transport of exudate and drug for treating heavily exuding wounds.

Pharmacological potentials of Rhamnus alaternus L. stem bark extract in vivo: antioxidant, analgesic, anti-inflammatory and wound healing effects.

Pharmacological potentials of Rhamnus alaternus L. stem bark extract in vivo: antioxidant, analgesic, anti-inflammatory and wound healing effects.

Pheretima: A system review of zoology, traditional applications, processing technology, chemical composition, pharmacologic activities, quality control and toxicity.

Pheretima: A system review of zoology, traditional applications, processing technology, chemical composition, pharmacologic activities, quality control and toxicity.

Antidiabetic and Wound Healing Effects of Methanol Extract of Aloe porphyrostachys Lavranos in Streptozotocin- Induced Diabetic Rats

Antidiabetic and Wound Healing Effects of Methanol Extract of Aloe porphyrostachys Lavranos in Streptozotocin- Induced Diabetic Rats