Wound Healing Effect Research Articles

Bacoside A: A Promising Medication for Treatment of Various Disorders

Abstract: Bacoside A is a triterpenoid saponin and nootropic herb that can be extracted from Bacopa monnieri Linn., Scrophulariaceae. It is a glabrous and succulent herb used traditionally for centuries in treating various illnesses, mainly as a cardiotonic and nerve tonic, and has been considered an eminent Ayurvedic medicine. Various studies reported that the herb consists of a wide range of potentially bioactive, phytochemical constituents with synergistic effects. Bacoside A is a major constituent of B. monnieri, with diverse biological functions and significant therapeutic potential, as given by various researchers and preclinical studies. It possesses various biological activities such as hepatoprotective, anti-osteoporotic, anti-ulcerogenic, anti-inflammatory, anti-tumor, wound healing, and neuroprotective effects. This review compiled the preclinical evidence-based studies and present knowledge of bacoside A's potential benefits and fruitful impact on human health, and we provided a comprehensive perspective of bacoside A in various disease treatments.

Construction of acidic microenvironment by Cu-TCPP nanozyme composited deprotonatable polymeric nanofibers for efficient antibacterial activity

The issue of bacterial resistance caused by conventional antibiotics has emerged as a major challenge in clinical treatment. Nanozymes, based on catalysis and devoid of bacterial resistance, provide a novel approach to eradicating bacteria. However, the practical potential of these nanozymes is limited by their low and readily pH-affected catalytic activity. Herein, the enzyme activity was optimized starting from the synthesis of nanozyme and the selection of carboxylate-containing polymers for the matrix. Specifically, various copper-tetrakis (4-carboxyphenyl) porphyrin (Cu-TCPP) were initially synthesized employing three copper sources, and among them, Cu-TCPP derived from Cu2O with higher catalytic activity was preferred. Subsequently, Cu-TCPP composite fibrous membranes were further obtained by electrospinning from their blend solutions with different polymers with distinct deprotonation capabilities. Thus, an acidic microenvironment conducive to the exertion of enzyme activity was constructed. The fabricated composite nanofibrous membrane could dissociate hydrogen ions to construct a localized acidic microenvironment even under weakly alkaline conditions, thereby enhancing the enzyme activity and improving the antibacterial effect. Further in vivo experiments demonstrated its favorable antibacterial ability and accelerated wound healing effect. The enhancement strategy of enzyme activity in this study brings inspiration for the design of nanozyme composited wound dressings and holds significant potential for clinical antibacterial applications

Vesicular phospholipid gels: A new strategy to improve topical antimicrobial dermatotherapy

Therapeutically effective and biocompatible dermal formulations that can ensure localization of a high level of antimicrobial drug at the site of action for an appropriate duration, while at the same time providing intrinsic reepithelization properties, are of particular importance for the treatment of infected and injured skin. The current research aimed to explore the potentials of using vesicular phospholipid gels (VPGs), semisolid formulations consisting of tightly packed liposomes (100–––200 nm), as innovative local depot drug vehicles for advanced topical dermatotherapy. Ciprofloxacin hydrocholoride (CPX) was selected as a model hydrophilic antibacterial drug and was loaded into several VPGs, differing in their composition. Various CPX-loaded VPGs (CPX-VPGs) were evaluated in vitro for the rheological and physicochemical characteristics, drug release profile, stability under in vivo mimicked conditions and during storage, skin permeability, biocompatibility with the epidermal cells, antibacterial efficacy and wound healing assay, to determine the optimal CPX-VPG for topical dermatotherapy. Viscosity and bilayers fluidity of VPGs affected the release of CPX from CPX-VPGs and its skin localization, limiting CPX percutaneous absorption. All CPX-VPGs exhibited even a 2-fold increase in anti-biofilm activity against both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate compared to the free drug, while showing no toxic effects on human keratinocytes in vitro. Based on the pronounced proliferative effects on keratinocytes, superior in vitro wound healing effect and drug localization on/inside the skin, CPX-VPGs containing chitosan and hydrogenated phospholipid proved to be the most promising for topical dermatotherapy. These findings, along with increased bioadhesiveness and the slow drug release, with CPX concentrations significantly above the minimum biofilm inhibitory concentrations for bacteria typical in infected wounds, would contribute not only to the improvement of the antimicrobial dermatotherapy, but also to reduction of the frequency of the drug administration.

Biocompatible Electrospun Hydrogel Fibers for Advanced Wound Healing Therapies.

Wound healing is a complex cascade and is governed through a number of crucial factors. Conventional wound dressing possesses numerous limitations which hinder wound healing process and may result in serious infections and even mortality. A lot of effort have been put in through researchers to develop a multifaceted dressing which can address these limitations and facilitate accelerated wound healing. Among various newly developed dressings, electrospun hydrogel nanofibers have emerged as a promising class of biomaterials for advanced wound care and tissue engineering applications. These biomimetic fibers closely mimic the architect of the native extracellular matrix, providing an optimal environment that facilitates cellular proliferation and fast generation required for effective wound healing. Electrospinning offers versatility in precisely controlling fiber attributes such as diameter, alignment, and surface morphology and can entrap a variety of drugs with high efficacy. Recently, such dressings have advanced through the incorporation of smart features such as stimuli-responsive components, real-time wound monitoring sensors, and smart closed-loop systems. The electrospun hydrogels are bestowed with extreme porosity, water-retention attribute, biocompatibility, and modified drug release which make them superior over other wound dressings. The review gives an insight of electrospun hydrogel fibers and their application in wound healing and the studies assessing wound healing potential with underlying mechanisms have been critically analysed. Electrospun hydrogel fibers have significant potential to revolutionize wound care through their biomimetic structure, versatile customization, and capacity for integrating therapeutic and sensing capabilities, outlining future research directions toward next-generation wound care products.

Monoclonal antibodies against jellyfish collagen.

Collagens are abundant structural proteins found in both mammalian and marine species, and attractive biomaterials used in various fields. Jellyfish collagen-based products have become increasingly popular because of their clinically proven health benefits such as the effects of skin wound healing and immune stimulation. To develop detection tools for jellyfish collagen, we generated four monoclonal antibodies, MCOL1, 2, 3, and 4, by immunizing mice with moon jellyfish collagen. The nucleotide and amino acid sequences of the variable regions of the monoclonal antibodies were determined. The antibody-binding kinetics toward collagens from moon jellyfish were evaluated using a surface plasmon resonance (SPR) biosensor, and the binding specificity was evaluated in comparison with binding to collagens from edible jellyfish, fish scales, and pig and cow skins. MCOL1, 3, and 4 specifically bound to moon jellyfish collagen, whereas MCOL2 bound to both moon and edible jellyfish collagens. Considering the results showing that the SPR responses of MCOL2 binding were greater than those seen with the other antibodies, MCOL2 could recognize the common and repetitive sequences of the two jellyfish collagens. Therefore, this monoclonal antibody will be most applicable for detecting jellyfish collagen.

Bioactive glass suspension hydrogel promotes wound healing by modulating fibroblasts.

The irritation and adhesion of wound healing biomaterials to wet wounds should be addressed for achieving effective wound healing. In this study, a stable multifunctional hydrogels (BGs/HA suspension gels) were prepared using superfine powder of bioactive glasses (BGs), the biocompatible materials hyaluronic acid (HA) and carbomer940, which had good adhesion and low irritation properties for use in moist complex wounds. The average particle size of BGs/HA suspension gels was 13.11 ± 0.29 μm, and the bioactive glass content was 15.8 ± 0.2% (m/m). The results of cell proliferation, cell migration, and immunofluorescence staining experiments showed that in the initial stage of wound healing, the ionic extract of BGs formulations promoted the proliferation and migration of L929 cells and induced the secretion of α-SMA and collagen I. In the final stage of repair, the ionic extract of the BGs formulation regulated the differentiation of fibroblast, which contributed to the reduction of pathological scar formation. In vivo experiments showed that the wound healing rate of BGs/HA suspension gels group exceeded higher than that of the conventional BGs superfine powder group. Although BGs/HA suspension gels were comparable to its commercially available counterpart (Dermlin paste) in promoting wound healing, it addressed the problem of localized irritation caused by the high pH and low adhesion of BGs products. This study confirmed the specific regulatory effect of BGs/HA suspension gels on L929 cells, which provided a reference for the clinical application of BGs in wound dressing.

Assessment of the Wound-Healing Effect of Hydrogels on an Experimental Burn Model in Rodents

Assessment of the Wound-Healing Effect of Hydrogels on an Experimental Burn Model in Rodents

Wound Healing Herbal Formulation from Essential Oil of Lemongrass (Cymbopogon citratus)

Untreated wound may lead to the infection. In some countries, plants are used to cure wound traditionally. Lemongrass (Cymbopogon citratus) has various bioactive compounds, including citronellol, limonene and geraniol that perform some pharmacological activities. In this study, the essential oil of lemongrass was designed into ointment formulation since lemongrass has been reported to have a wound healing effect in mice. The wound healing ointment made from different concentration of the essential oil of Cymbopogon citratus (F1: EOCC 5%, F2: EOCC 10% and F3: EOCC 15%) using a water-removable base and the characteristic as well as the stability (texture, color, smell, homogeneity, and pH) after been stored for 14 days was also evaluated. The result showed that all formulation (F1, F2, and F3) of wound healing formulation revealed good texture, consistency, odor, color, homogeneity and compatible to the skin until 14 days of storage. However, F3 (EOCC 15%) has a strong lemongrass smell compare to the other formulations.

Genus Stachys-Phytochemistry, Traditional Medicinal Uses, and Future Perspectives.

The genus Stachys represents one of the most extensive genera within the subfamily Lamioideae and ranks as one of the largest genera in the Lamiaceae family. Many Stachys species are associated with a rich history in traditional medicine across various cultures, and their extracts and essential oils are rich in non-volatile and volatile compounds. Because of their complex profile of bioactive substances, Stachys members are considered to possess an extensive spectrum of therapeutic properties, including antioxidant, anti-inflammatory, analgesic, antibacterial, cytotoxic, and wound-healing effects, as well as benefits for memory enhancement, lipid profile regulation, blood glucose control, and weight management. Despite the wide distribution and chemical diversity of the genus, studies on its biological activities remain limited. The aim of this review is to summarize the most relevant data from studies on the bioactive compounds, traditional uses, and pharmacological properties of Stachys species found in databases such as Science Direct, PubMed, and Scopus. Specific keywords were used in the search strategy, including "bioactive compounds", "Stachys", "Lamiaceae", "Stachys extract", "Stachys essential oil", "traditional uses", "chemical composition", "therapeutic potential", "clinical trials", "in vivo studies", "in vitro studies". The search strategy was performed according to the guidelines for Preferred Reporting Items for Systematic Reviews and Meta-Analyses. Data from 171 studies were included. The manuscript highlights the drug-discovery potential of Stachys species, emphasizing their potential applications in medicine, cosmetics, dietary supplements, and the food industry. Moreover, it provides important data that could assist Stachys research programs.

Whey proteins from camel's milk have higher in vitro wound-healing effect than whey proteins from cow's milk.

The effectiveness of whey obtained by either enzyme (sweet) or acid treatment on wound healing remains unclear. This study investigated the effectiveness of camel and bovine whey prepared enzymatically (CSW and BSW) or by pH reduction (CAW and BAW). After removing the cream from milk, HCl or rennet was used to remove casein, resulting in acid or sweet whey, respectively, followed by lactose removal using dialysis. Casein removal was verified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Wound-healing activity was measured in vitro on HT-29 cells by scratch assay. All four whey samples (0-1000 mg L-1) were applied on the cells, and the closure of the cell-free scratched areas was monitored for 48 h. All whey samples increased the cell migration significantly (P < 0.05) to help close the cell-free areas as an indication of wound healing compared to the negative control. However, the closure amounts between the highest dose (1000 mg L-1) and the control were not significantly different (P > 0.05). Acid whey samples significantly (P < 0.05) elevated the closure speed compared to the sweet whey samples. The highest closure percentage (64.69%) was achieved after treatment with 10 mg L-1 CAW for 48 h. Between the sweet whey samples, BSW was significantly (P < 0.05) more effective in closing the cell-free zone compared to CSW. This study investigated the wound-healing potential of camel and bovine whey in vitro by comparing their effects on HT-29 cell migration. CAW showed the greatest activity and may find uses as a treatment agent in wound healing. © 2024 Society of Chemical Industry.

Effects of Matricaria aurea extract in excisional wound healing on albino rats

The Asteraceae herbal plant Matricaria aurea (Loefl.) Sch.Bip is well known for its use in treating gastrointestinal, cardiac, pulmonary, rheumatologic and many other ailments; nevertheless, wound care has not yet received scientific validation. This study was done to evaluate the efficacy of M. aurea topical formulation on the rate of wound healing and contraction and the histopathological properties of the healing tissue in a rat model of an excisional wound. A total of 45 male rats with excisional wounds were enrolled in the study. The rats were divided into three groups, each with 15 male rats weighing approximately 200–250 g. The first group received treatment with M. aurea, the second group received fusidic acid cream and the third group received normal saline. The treatment was administered for 15 days while photographs were taken every other day, the diameters were measured and a histopathologic examination was conducted. There was a significant accelerated effect for wound healing, as evidenced by the inflammatory cells and diameters reducing to almost the same degree in the M. aurea and fusidic acid groups as compared to the control group (p&lt;0.05). In vivo, the healing of wounds in albino Wister rats is demonstrated by the ethanolic extract of M. aurea. The findings, which show enhanced contraction, epithelization and tensile strength, significantly imply that wound healing promotion and acceleration can be achieved based on faster healing as compared to negative control and similar to standard control (fusidic acid) wounds.

A versatile natural gelatin-based hydrogel for emergency wound treatment through hemostasis, antibacterial, and anti-inflammation

Emergency wounds are often accompanied by bacterial infection, oxidative stress, and excessive inflammation due to the inability to quickly close and stop bleeding, resulting in chronic wounds that are difficult to heal. Clinically, surgical suturing is the fastest method for wound closure, but it is only suitable for wounds with small bleeding volumes and causes unsightly scar formation. Consequently, there is a critical need for hemostatic dressings versatile enough to address a spectrum of diverse and intricate wounds, especially in emergency scenarios. In this study, we constructed a unique versatile natural gelatin-based hydrogel with hemostasis, antibacterial, and anti-inflammation properties. The hydrogel was composed of 4-(4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy) butyrylethylenediamine-modified methacrylated gelatin (GelMA-NB) and epigallocatechin gallate-grafted polylysine (EPL-EGCG), which imparts adhesion, antibacterial and antioxidant properties to the hydrogel. Simultaneously, the hydrogel was loaded with GelMA microspheres encapsulating natural resveratrol (RES@GM). This combination not only exhibited outstanding hemostatic capabilities but also preserved the anti-inflammatory potential of RES. In different animal models, the hydrogel exhibited outstanding hemostatic and wound healing effects, down-regulated the expression of IL-1βto promote inflammatory regulation and potential for angiogenesis and anti-scar. In conclusion, unique versatile natural gelatin-based hydrogel suitable for various complex wounds provides a promising strategy for emergency wound dressing applications.

The effect of cold atmospheric plasma on viability of osteoblasts and expression of RANKL and OPG genes in medium with bisphosphonates.

Medication-related osteonecrosis of the jaw is a rare but severe complication with challenging treatment protocols. Cold atmospheric plasma (CAP) has shown promising effects in wound healing, cell proliferation and viability. This study investigated the effect of CAP on osteoblasts in a culture medium containing bisphosphonates. Pilot study was designed to determine the optimal setting of CAP. MG-63 cells were exposed to zoledronic acid at a concentration of 10 micromolar for 72h. Study groups with the best MTT assay results, were chosen for assessing OPG and RANKL genes by RT-PCR. Cell viability was significantly higher in groups 9kV.1mm.90s, 10kV.1mm.60s, and 12kV.1mm.60s (P < 0.05). Expression of RANKL in these groups was significantly lower than the positive control group (medium culture without zoledronic and plasma treatment) and higher than the BP group (culture with zoledronic without plasma treatment), except for the 12kV.1mm.60s group, which did not differ significantly from the positive control group (P > 0.05). OPG decreased in all test groups compared to BP (p < 0.05), except for the 12kV.1mm.60s group, which did not significantly differ from BP (P > 0.05). RANKL/OPG ratio in groups 9kV.1mm.90s and 12kV.1mm.60s significantly increased compared to BP (P < 0.05).CAP treatment may enhance the viability of osteoblasts exposed to bisphosphonates, increase their activity levels, enhance osteoclast activity, and improve bone turnover rates.

Chemical composition and pharmacological aspects of Malaysian stingless bee propolis: An up‑to‑date systematic review.

Propolis is a sticky substance produced by stingless bees for construction and defence of their hive. It has notable anti-inflammatory, antioxidant, antibacterial, antifungal, anti-hyperglycemic, and wound healing effects. The present review summarised and examined the phytochemical properties, mode of action and current research prospects of Malaysian propolis. A database search using Google Scholar, Web of Science and ScienceDirect generated 780 references; 30 relevant articles were included in the present review, of which 23 were in vitro studies and 7 were in vivo or animal studies. Propolis demonstrated antioxidant, antibacterial, antifungal, anti-inflammatory and anti-hyperglycemic properties, indicating potential as a wound healing agent. Despite favourable findings, due to the scarcity of studies in the literature, more in-depth research and clinical validation on the synergistic effects, efficacy and optimum dosage of propolis are needed.

Bagworm Silk‐Mimetic Protein Fibers with Extraordinary Stiffness via In Vivo Polymerization and Hierarchical Self‐Assembly

AbstractBagworm silk proteins, which contain crystalline structures with large β‐nanocrystal sizes, are ideal candidates for biosynthetic high‐performance fibers. However, the extremely high glycine content and greater molecular weight limit their heterologous expression efficiency and further application exploration. Here, a multi‐module assembly strategy is developed to engineer novel chimeric structural proteins by incorporating the mechanical functional domains of bagworm silk proteins with the C‐terminal self‐assembly domains of spider silk proteins. By selecting a single repetitive unit of the functional region of bagworm silk proteins, the challenge of low heterologous expression efficiency is successfully addressed. Furthermore, the content and ordering of β‐sheet structure are enhanced in the chimeric proteins through the alignment mediated by the spider silk C‐terminal domain and ligation facilitated by split inteins, resulting in a remarkable Young's modulus of ≈15 GPa. This surpasses many artificial protein fibers, synthetic polymer fibers, and even natural spider silk. Notably, these protein fibers are drawn into surgical sutures and demonstrate superior wound healing effects compared to clinical suture in a skin wound model. This research presents a novel strategy for developing high‐performance protein fibers, which will expand the scope of their mechanically demanding applications.

Pressure Ulcers and Nutrients: From Established Evidence to Gaps in Knowledge.

Pressure ulcers (PUs) are caused by continuous pressure or friction on the skin that damages tissue, especially over bony prominences. A critical factor in the development and progression of PUs is poor nutritional status, which often involves deficiencies in essential nutrients such as proteins, vitamins (A, C, D, E, K, and the B complex), and trace elements (including zinc, selenium, copper, iron, and manganese). These micronutrients are vital for effective wound healing, as they play significant roles in cellular repair, immune function, and tissue regeneration. Laboratory tests for serum albumin, prealbumin, transferrin, retinol-binding protein, and anthropometric measures like height, weight, and body mass index (BMI) are used to evaluate a patient's nutritional status. Screening tools such as the Mini Nutritional Assessment (MNA), Malnutrition Universal Screening Tool (MUST), LPZ questionnaire, and Subjective Global Assessment (SGA) are commonly employed. Emerging evidence from various studies, including in vitro, in vivo, and clinical trials, underscores the importance of personalized nutritional interventions in managing PUs. Unlike generic dietary plans, tailored nutrition that addresses the specific needs of individuals shows greater potential in promoting wound healing and improving clinical outcomes. This synthesis of existing research highlights the critical influence of micronutrients on the healing process of PUs. It suggests that a personalized approach to nutrition, which takes into account individual patient requirements and deficiencies, is likely to be more effective than a one-size-fits- all strategy in the management of these complex wounds.

Wound-Healing Activity of Solvent Fractions and Antiinflammatory Activity of Crude Extract and Solvent Fractions of Acokanthera schimperi Schweinf (Apocynaceae) Leaves in Mice Model

BackgroundIn Ethiopia, Acokanthera schimperi is frequently used to treat wounds and other conditions. Nothing has been published to date on the anti-inflammatory properties of A. schimperi leaves or the wound-healing effects of solvent fractions, despite a report on the crude extract's ability to heal wounds being available in the literature. PurposeTherefore, this experiment has been initiated to look into the solvent fractions' ability to heal wounds and the anti-inflammatory properties of the A. schimperi leaf 80% methanol extract and its fractions in mice. Study designThe experimental study design was conducted to evaluate wound healing and the anti-inflammatory properties of the A. schimperi leaf 80% methanol extract and its fractions in mice. MethodsThe plant's leaf crude extract was fractionated using ethyl acetate, chloroform, and distilled water. The resulting fractions were then combined with a simple ointment base to make an ointment at 5% and 10% w/w. Next, using excision and incision wound models, the ointments' ability to promote wound healing was assessed. The excision model was utilised to estimate wound contraction and epithelization time, whereas the incision model was utilised to measure tensile strength. By dissolving 1% carrageenan in 0.9% saline (w/v) and giving oral dosages of the crude extract and solvent fractions (100, 200, and 400 mg/kg), the anti-inflammatory efficacy of the leaves was assessed concurrently with a carrageenan-induced hind paw edema model. ResultsSignificant wound healing activity was seen in both models in wounds treated with 5% and 10% (w/w) solvent fraction ointment, as shown by higher tissue breaking strength (with 51.38% maximum effect by 10% (w/w) ethyl acetate fraction ointment), shorter epithelization times (within 15.92 days by ethyl acetate fraction ointment 10%), and increased wound contraction (99.40% by ethyl acetate fraction ointment 10%). The extract (hydromethanolic) and solvent fractions also showed notable dose-related decreases in inflammation. ConclusionThe study's findings supported A. schimperi's traditional use as a wound healing agent by showing that the solvent fractions of the plant's leaves promoted wound healing, at least in part, through their anti-inflammatory effect.

Effectiveness of Wound Irrigation Using Hypochlorous Acid Liquid on Healing of Diabetic Foot Wounds

Background: Wound cleansing is crucial in wound healing, depending on the method and type of solution used. Hypochlorous Acid is one of the wound irrigation options that can be applied in this process. Objective: This study aims to evaluate the effectiveness of wound irrigation using Hypochlorous Acid (HOCL) on diabetic foot wound healing. Methods: This quantitative research employs a quasi-experimental design, involving 13 diabetic foot wound patients selected through total sampling. Interventions were conducted over five weeks, totaling ten sessions. The Bates-Jensen Wound Assessment Tool (BJWAT) was utilized to assess wound healing, and data were analyzed using SPSS 25, applying paired T-tests to evaluate pre-and post-intervention differences. Results: A statistically significant improvement in diabetic foot wound healing was observed, marked by a p-value = 0.000, highlighting the potential of HOCL in promoting effective wound healing. Conclusion: HOCL wound irrigation significantly enhances diabetic foot wound healing

Elastic and recoverable sponges based on collagen/yeast β-glucan for quick hemostasis

In this investigation, we aimed to engineer sponges with exceptional mechanical and hemostatic capabilities for effective wound healing. By combining collagen, a stiff fibril protein in ECM, with β-glucan, an elastic and triple-helical polysaccharide from yeast cell wall, we prepared a series of composite sponges, designated as CY sponges. This material exhibited a uniform pore structure, displaying enhanced elasticity and shape recovery ability compared to pure collagen sponges. Also, the incorporation of Yeast β-glucan (YG) significantly improved the fluid absorption ability and stability of the sponges. In vitro hemostasis tests demonstrated that the CY sponges exhibited a notably lower in vitro coagulation index (19.21 %) compared to the collagen control (64.84 %), accompanied by superior erythrocyte (64.64 %) and platelet (64.95 %) adhesion properties. Animal studies further substantiated the sponge's hemostatic efficacy, as CY40 led to a reduction in average bleeding volume by 25.26 % and 28.97 %, and a shorter hemostatic time by 31.70 % and 30.77 % compared to collagen, indicating accelerated wound healing. These findings suggest that the addition of yeast β-glucan into collagen sponges can improve their elasticity, shape recovery ability, hemostatic performance and wound repair ability.

Evaluation of wound healing activities of Vitex doniana (Black Plum) ethanol fruit extract

In this study, the folkloric use of Vitex doniana fruit extract for the treatment of wounds was evaluated. The mesocarps of the fresh fruits were removed, oven-dried at 40°C and pulverized. The powdered material (800 g), was exhaustively macerated in 80 % ethanol, filtered and dried in a water bath at 40°C. Phytochemical screening and acute dermal toxicity of the extract were investigated. Herbal ointments (10 and 50 %w/w) were formulated using soft paraffin base. The wound-healing effect of the extract was carried out using twenty albino rats divided into four groups (n=5). Groups I (soft paraffin) and II (10 %w/w Povidone-iodine) served as negative and positive controls, respectively. Groups III and IV were topically treated with 10 %w/w and 50 %w/w of the herbal ointment respectively. Treatments and wound grading were assessed once daily and every 4 days, respectively, as well as the timeframe for percentage wound contraction and epithelialization Both the 10 %w/w and 50 %w/w herbal ointments exhibited a concentration-related significant (P&lt;0.05) wound contraction when compared to the control. Epithelialization period of 50 %w/w extract was less than that of the standard drug. The wound closure area of 50 %w/w extract was &gt;positive control &gt;10 %w/w extract &gt; negative control. Histological study of the wounds on the 16th day indicated no sign of dermal toxicity. Some secondary metabolites such as alkaloids and tannins were present. In conclusion, the folkloric use of the fruit extract of V. doniana for the treatment of wounds may therefore be justified