Event Abstract Back to Event Morphine withdrawal anxiety influenced by pituitary adenylate cyclase-activating polypeptide (PACAP) in mice N. Lipták1*, R. Dochnal2, A. Babits1, K. Csabafi1, G. Tóth3 and G. Szabó1 1 University of Szeged, Departments of Pathophysiology, Hungary 2 University of Szeged, Child and Adolescent Psychiatry, Hungary 3 University of Szeged, Medical Chemistry Faculty of Medicine, Hungary Introduction: In this study the effect of PACAP on morphine withdrawal anxiety was investigated in mice. The elevated plus maze (EPM) and jump-test were used to assess morphine withdrawal anxiety. Morphine withdrawal is known to be anxiogenic in rats. In contrast to rats, in our research naloxone precipitated withdrawal induced anxiolytic effect. Methods: CFLP male mice were used. Mice were treated twice daily with s.c. injections of morphine or saline and once daily with PACAP (500 ng/2 μl, i.c.v.) or ACSF. Treatments for EPM: day 1: 10 mg/kg, day 2: 20 mg/kg, day 3: 40 mg/kg; final dose day 4: 20 mg/kg. Treatments for jump-test: day 1: 20 mg/kg, day 2: 40 mg/kg, day 3: 60 mg/kg, day 4: 80 mg/kg, final dose day 5: 100 mg/kg. Results: EPM: naloxone (0.2 mg/kg, s.c.) administration in morphine dependent mice significantly increased the open-arm time/total time rate and the number of entries in arms compared with the control mice. PACAP had no significant effect on open-arm time/total time rate, but significantly increased the total number of entries compared with the naloxone precipitated withdrawal morphine-dependent mice. Jump-test: after naloxone (1 mg/kg, s.c.) injection, morphine-dependent mice treated with PACAP exhibited significant decrease in jumping behavior and significant increase body temperature compared morphine dependent mice. Conclusion: PACAP compensated the effects of morphine withdrawal; these results may help us to understand morphine withdrawal anxiety. Acknowledgements This work was supported by ETT 355-08/2009 and TÁMOP 4.2.1B. Keywords: Behaviour and cognition, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Behaviour and cognition Citation: Lipták N, Dochnal R, Babits A, Csabafi K, Tóth G and Szabó G (2011). Morphine withdrawal anxiety influenced by pituitary adenylate cyclase-activating polypeptide (PACAP) in mice. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00172 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. N. Lipták, University of Szeged, Departments of Pathophysiology, Szeged, Hungary, liptakn@freemail.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers N. Lipták R. Dochnal A. Babits K. Csabafi G. Tóth G. Szabó Google N. Lipták R. Dochnal A. Babits K. Csabafi G. Tóth G. Szabó Google Scholar N. Lipták R. Dochnal A. Babits K. Csabafi G. Tóth G. Szabó PubMed N. Lipták R. Dochnal A. Babits K. Csabafi G. Tóth G. Szabó Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.