White Rabbits Research Articles

A packet-based precise timing and synchronous DAQ network for the LHAASO project

Aiming to high sensitivity and wide spectrum of cosmic ray detection, the Large High Altitude Air Shower Observatory (LHAASO) project consists of over 10,000 detectors of different types, spreading over 1.2km2. To precisely reconstruct the air shower events with high angular resolution, all detector electronics and digitizers should work in synchronous acquisition mode with a global timing error less than <5ns (rms). This large scale and high precision timing requirement exceeds the capability or feasibility of traditional methods such as GPS and echo-delay calibration. Recently an emerging method named White Rabbit (WR) was proposed and demonstrated as a cost-effective solution that combines Gigabit Ethernet data transfer and subnanosecond precision timing transfer over the same fiber media. Furthermore, the White Rabbit network also enables direct digitization and trigger-less data acquisition mode for each detector, which will greatly reduce the complexity and improve the performance of the data acquisition (DAQ) system. In this paper we will demonstrate the design and development status of a WR timing and DAQ network in the LHAASO project.

The White Rabbit

The life of an adjunct is never slow. This essay looks into the professional and personal life of a community college teacher working at multiple colleges who wonders, like many others, how she will hold it all together and provide the quality of teaching that her students deserve.

Sodium Hyaluronate Decreases Ocular Surface Toxicity Induced by Benzalkonium Chloride–Preserved Latanoprost: An In Vivo Study

To investigate the effects of sodium hyaluronate (SH) on ocular surface toxicity induced by benzalkonium chloride (BAC)-preserved latanoprost. Twenty-one white rabbits (42 eyes) were randomly divided into three groups. The control group was untreated. The two experimental groups were treated with 0.02% BAC-containing latanoprost once a day combined with unpreserved 0.3% SH or PBS three times daily for 60 days. Schirmer test, fluorescein and rose bengal staining, and conjunctiva impression cytology were performed on days 0, 31, and 61. Apoptosis of conjunctival epithelium was detected by TUNEL assay on day 61. Conjunctival inflammation was evaluated with light microscopy. Cornea and conjunctiva ultrastructure were observed by electron microscopy. Compared with the control group, the PBS-treated latanoprost group showed increases in fluorescein and rose bengal scores, decreases in Schirmer scores, and goblet cell density (GCD) on days 31 and 61. Increases in inflammatory and apoptotic cells in conjunctiva, and ultrastructural disorders of the cornea and conjunctiva were also observed on day 61. Compared with the PBS-treated latanoprost group, the SH-treated latanoprost group showed decreases in fluorescein and rose bengal scores, and increases in Schirmer scores and GCD on days 31 and 61. Decreases in inflammatory and apoptotic cells in conjunctiva and amelioration of ultrastructural disorders were also observed. Topical application of SH significantly decreased the ocular surface toxicity induced by BAC-preserved latanoprost. As a vehicle or neutralizing material, SH could be proposed to reduce ocular toxicity and protect the ocular surface in long-term BAK-preserved antiglaucoma medication treatment.

Histomorphometrical and radiological comparison of low-level laser therapy effects on distraction osteogenesis: experimental study

Distraction osteogenesis (DO) is the application of traction to the callus formed between bone segments and stimulation of bone formation by creating stress on the callus with this traction. Shorten the duration of DO and increasing the capacity of bone formation is important to prevent the possible complications of DO. For this reason, it was considered that low-level laser therapy (LLLT) may affect positively DO and it can decrease the complication range by shortening the period. Unilateral mandibular distractors were applied on 16 female white New Zealand rabbit to prove this hypothesis with micro CT, plain radiograph and histomorphometric analyses. Eight rabbits were applied LLLT with GaAlAs laser on the distraction area during the distraction period. On the post-distraction 28th day, four rabbits from study group and four rabbits from control groups were sacrificed. The rest of the rabbits were sacrificed on post-distraction 56th day. As a result of this study, significant positive effects of LLLT on post-distraction 28th day were revealed with all analyses. In histomorphometrical analyses, new bone formation was significantly higher in short-term laser applied group comparing to that of short-term control group (p = 0.029). In both microCT and plain radiograph, the highest radioopacity values were observed in short-term laser group when compared with that of the controls (p = 0.043 and p = 0.025, respectively). Even though LLLT increased the healing capacity on short-term, it was not sufficient on long-term (post-distraction 56th day) healing. LLLT application on distraction period, activate healing on bone so it may decrease DO period. The result of this study should be supported with clinical studies and the most effective laser source, dose and application time should be revealed with experimental and clinical studies.

A Novel Application of Biosynthetic Tissue-Engineered Tridimensional Implant on Large Tendon Defects: A Comprehensive, Detailed, In Vivo Investigation with Significant Clinical Value

This study was designed to investigate the effect of a biosynthetic implant on tendon healing in vivo. Fifty white New Zealand male rabbits were randomly divided into two groups, namely treated (n = 25) and control (n = 25) groups. A large gap was created in the Achilles tendon and was maintained by Kessler pattern. In the treated group, the implant was inserted in the injured area. No implant was used in the control group. Contrast radiography, hematology, and clinical examination were conducted during the course of the experiment. The animals were euthanized at 60 days post injury (DPI) and their Achilles tendons were subjected to the gross, histopathologic, and biomechanical analyses and the hydroxyproline content of these tendons was also evaluated. Another five treated animals, as a pilot group, were used to define the inflammatory reaction at 10 DPI. Severe inflammatory reaction was initiated by the partially degraded implant, at 10 DPI. However, at 60 DPI, the inflammation subsided, the implant was mostly removed but a few small remnants were still present in the injured area. The newly formed tendon, properly aligned along the longitudinal axis of the Achilles tendon replaced the collagen implant. In the control tendons, a loose areolar connective tissue which tightly adhered to the peri-tendinous tissue was the only regenerated structure in the injured area. At this stage, the treated tendons showed significantly higher ultimate strength (p = 0.001), yield strength (p = 0.001), and stiffness (p = 0.001) compared with the control ones. Application of the biosynthetic implant was a safe and effective option in managing the large tendon defects and could be considered as a substitute for autografts in clinical practice.

Effect of vagus nerve stimulation on focal transient cerebral ischemia and reperfusion in adult male white new zealand rabbits

Background: Oxidative stress has been implicated in the pathophysiology of cerebral ischemia. Pentraxin-3 plays an important role in innate immune responses and in inflammatory diseases. Our aim was to evaluate pentraxin-3 serum level on focal transient cerebral ischemia and reperfusion model in rabbits and to assess the anti-inflammatory and anti-oxidant effects of vagus nerve stimulation. Materials and methods: Focal transient cerebral ischemia and reperfusion was induced by occlusion of the right common carotid artery for 2 hours followed by reperfusion for one hour. Stimulating electrodes were implanted on the cervical part of the right vagus nerve. Vagus nerve stimulation was started 30 min following right common carotid artery ligation for a period of one hour. The stimulation signals were delivered every five minutes for 30 seconds. All the procedures were duplicated but no stimulus was delivered in the control group. Serum level of pentraxin-3, lipid peroxide and total thiols were determined at baseline, at end of ischemia and at end of reperfusion and the animal decapitated and neuronal damage was evaluated. Results: We found that vagus nerve stimulation caused reduction of the ischemic features with revival of the cell shape and size. It also resulted in decreased serum levels of pentraxin-3 and lipid peroxide whereas the level of total thiols was increased. Conclusion: We concluded that the observed diversity in pentraxin-3, lipid peroxide and total thiols serum levels in cerebral ischemia and reperfusion may reflect relative roles in the biology. Anti-inflammatory and anti-oxidant role of vagus nerve stimulation in cerebral ischemia and reperfusion may represent a marker of altered cerebral function, and may provide potential therapeutic applications.

GINGKO suppresses atherosclerosis through downregulating the expression of connexin 43 in rabbits

Introduction: Ginkgo biloba extract (GBE) EGb761 is widely used for cardiovascular prevention. Here, we investigated the effects of GBE on atherosclerotic lesion development in rabbits with a high-fat diet. Material and methods: Forty New Zealand white male rabbits were randomly divided into four groups. The first two were the normal diet group (C) and the high-fat group (HF). The remaining two groups were those who received a high cholesterol diet supplemented with either the standard drug (simvastatin 2 mg/kg/day) or GBE (3 mg/kg/day). At 12 weeks, histopathological and chemical analyses were performed. Results: Plasma lipid measurement showed that GBE inhibited high-fat dietinduced increase of serum triglyceride (TG), total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C) by 59.1% (0.9 ±0.2 4 mmol/l vs. 2.2 ±0.4 mmol/l), 18.2% (31.1 ±1.4 mmol/l vs. 38.0 ±0.4 mmol/l) and 15% (28.9 ±1.3 mmol/l vs. 34.0±1.0 mmol/l), respectively, at 12 weeks (p < 0.01). The en face Sudan IV-positive lesion area of the aorta in the GBE group (51.7 ±3.1%) was significantly lower compared with that in the HF group (88.2 ±2.2%; p < 0.01). The mean atherosclerotic lesion area of the GBE group was reduced by 53.2% compared with the HF group (p < 0.01). Immunohistochemistry and western blot analysis showed that GBE markedly suppressed high-fat dietinduced upregulation of connexin 43 (Cx43) in rabbits (p < 0.01). Conclusions: Thus, our study revealed that GBE prevented atherosclerosis progress through modulating plasma lipid, suppressing atherosclerotic lesion development, and attenuating the expression of Cx43 protein.

Mean central corneal thickness and corneal power measurements in pigmented and white rabbits using Visante optical coherence tomography and ATLAS corneal topography

To document central corneal thickness (CCT) and power measurements in pigmented and white rabbits using Visante anterior segment optical coherence tomography (AS-OCT) and the Humphrey ATLAS Corneal Topography System (HACTS). Fourteen female rabbits (seven pigmented and seven white rabbits) were involved in this study. Twenty-eight eyes underwent AS-OCT and HACTS examination. Central corneal thickness, corneal power in the steepest and flattest meridians, astigmatism, pupil diameter (PD), and white-to-white (WTW) were calculated. The CCT was 390 ± 14.2 and 373 ± 7.2 μm for pigmented and white rabbits, respectively. The CCT values showed statistical difference between the two breeds (P = 0.017). The corneal power in the steepest meridian was 44.6 ± 1.9 Diopter (D) in pigmented rabbits and 47.8 ± 1.5 D in white rabbits. The corneal power in the flattest meridian was 44.0 ± 2.1 D in pigmented rabbits and 47.4 ± 1.5 D in white rabbits. The astigmatism, PD, and WTW values were 0.6 ± 0.22 D, 7.9 ± 0.9, and 14.5 ± 0.1 mm in pigmented rabbits, respectively. The corresponding values in white rabbits were 0.4 ± 0.23 D, 7.5 ± 0.5, and 13.5 ± 0.2 mm. There was a statistically significant difference in corneal power in the steepest and flattest meridians and for WTW. No such difference was observed for astigmatism or PD between them. Differences exist in the CCT, corneal power, and WTW between 5-month-old pigmented and white rabbits. Such difference should be considered when designing cornea-related experiments in rabbits.

BMW art guide by independent collectors: the first global guide to private and publicly accessible collections of contemporary art

The BMW Art Guide by Independent Collectors presents more than 160 private collections of contemporary art that are open to the public-both large and small, prominent and little known. Succinct portraits of the collections and numerous color illustrations take the reader to over thirty countries, often to out-of-the-way regions or urban districts. This practical guide is the first publication to be coproduced by BMW and the Independent Collectors, the international online network for collectors of contemporary art. Collectors, dealers, artists, and journalists contributed to the extensive research required for the compilation of this new standard work. A comparable list of international private collections has never before been assembled, either in book form or on the Internet, and some of these collections are opening their doors for the first time to art lovers and experts. Collections featured (selection): Museum of Old and New Art (MONA), White Rabbit - Contemporary Chinese Art Collection, Lyon Housemuseum, Essl Museum - Kunst der Gegenwart, Museum Liaunig

White Rabbit: Sub-nanosecond timing over Ethernet

The White Rabbit (WR) project is a multi-laboratory, multi-company effort to bring the best of the data transfer and the timing world together in a completely open design. WR is a fully deterministic Ethernet-based network for general purpose data transfer and synchronization. The aim is to enable the synchronization of a large number of nodes with sub-nanosecond accuracy and picosecond jitter over long lengths of fibre. The key technologies used are physical layer syntonization (clock recovery) and the Precision Time Protocol (IEEE 1588). WR generates sub-nanosecond synchronous precision timing in all nodes by continuous tracking and compensating the transmission delays. We give an overview of the WR project and describe the design goals and specifications of the project. The WR switch and the (user) node which are the central components of the WR system and real timing measurements of prototypes of WR hardware are presented.

Dynamic Mechanical Properties of Tissue after Long-Term Implantation of Collagen and Polypropylene Meshes in Animal Models

Purpose: Pelvic floor reconstructive surgery has grown significantly in recent years. A wide variety of available types of meshes exist but the safety and success has not been adequately proven. We sought to evaluate the effects on dynamic biomechanical properties of tissue after long-term implantation of synthetic and biological grafts. Methods: A total of 96 New Zealand white female rabbits (approximately 3 kg) were used, 72 of which were surgically implanted with acellular, collagen mesh (n = 36) or nonabsorbable monofilament polypropylene mesh (n = 36). There was a no mesh-rupture of fascia group (n = 12) and a second, no-mesh, no-fascia rupture control group (n = 12). In the 59 rabbits, of 72 (13 died) tissue was harvested 3 months (n = 24), 6 months (n = 23) and 9 months (n = 12) later, while in the fascia rupture group, tissue was harvested 6 months later. Tissue samples (2 × 2 cm) underwent dynamic mechanical analysis (DMA) testing during which the dynamic rigidity and tissue damping capacities were measured. The statistical analysis was performed with General Linear Model with Tukeys post hoc testing (sPss v.17.0). Results: With respect to mesh type, the rabbit tissue in which polypropylene mesh was used showed the greatest dynamic rigidity. Those with biological mesh delivered the lowest rigidity results, while the two other groups had almost similar behavior. The meshes exhibited their highest relative dynamic tissue stiffening effect at 9 months. Conclusions: Biological mesh causes lower tissue rigidity, resulting in inferior mechanical response and thus seems to be inferior to polypropylene.

Characterization of a Syrian Chickpea chlorotic stunt virus strain and production of polyclonal antibodies for its detection

Reverse transcription-polymerase chain reaction analysis with two primer sets of luteoviruses was used to characterize an isolate of Chickpea chlorotic stunt virus (CpCSv, genus Polerovirus , family Luteoviridae ) (SC402-08) collected from Lattakia, Syria, during the 2007‒2008 chickpea growing season. Sequence analysis revealed that the coat protein gene of the isolate shared nucleotide sequence identities ranging from 97 to 98% with the CpCSv isolates from Egypt, morocco and Syria. The capsid protein was separated as a protein of approximately 20 kDa in sodium dodecyl sulphate polyacrylamide gel electrophoresis, and was visually detected by its reaction with CpCSV monoclonal antibody in Western blot. SC402-08 isolate of CpCSV was purified from faba bean-infected plants, and yielded 112–182 μg of purified virions kg-1 of infected tissue. The purified preparation was injected into a white rabbit, and an antiserum was obtained and used to detect CpCSv in infected tissues by tissue-blot immunoassay. The antiserum obtained was able to detect CpCSv by the immunoassay up to a dilution of 1:1,024,000.

Precision measurement of the neutrino velocity with the ICARUS detector in the CNGS beam

Abstract During May 2012, the CERN-CNGS neutrino beam has been operated for two weeks for a total of ~1.8 × 1017 p.o.t., with the proton beam made of bunches, few ns wide and separated by 100 ns. This beam structure allows a very accurate time of flight measurement of neutrinos from CERN to LNGS on an event-by-event basis. Both the ICARUS-T600 PMT-DAQ and the CERN-LNGS timing synchronization have been substantially improved for this campaign, taking advantage of additional independent GPS receivers, both at CERN and LNGS as well as of the deployment of the “White Rabbit” protocol both at CERN and LNGS. The ICARUS-T600 detector has collected 25 beam-associated events; the corresponding time of flight has been accurately evaluated, using all different time synchronization paths. The measured neutrino time of flight is compatible with the arrival of all events with speed equivalent to the one of light: the difference between the expected value based on the speed of light and the measured value is δt = tof c −tof ν = 0.10 ± 0.67stat. ± 2.39syst. ns. This result is in agreement with the value previously reported by the ICARUS Collaboration, δt = 0.3 ± 4.9stat. ± 9.0syst. ns, but with improved statistical and systematic accuracy.

Does The Titanium Plate Fixation of Symphyseal Fracture Affect Mandibular Growth?

The effect of metallic fixation on growth is a major concern in children and is not yet clear. The aim of this study was to evaluate the effect of metallic fixation of mandibular symphyseal fracture on mandibular growth.Eighteen 90-day-old growing white New Zealand rabbits weighing 1.6 to 2.5 kg were included in this study and divided into 2 groups of 9 subjects. In the experimental group, animals underwent mandibular osteotomy, simulating a symphyseal fracture on the midline of mandibular symphysis. The bone segments were fixed with microplates and microscrews (1.6 mm).In the control group, the same surgical incision without performing symphyseal osteotomy was conducted, and 2 screws were inserted on each side of the symphyseal midline.Digital cephalometric and submentovertex radiographs were taken before the operation and at postoperative 6 months for each animal in 2 groups, and cephalometric measurements were performed. The distance between the centers of the head of 2 screws measured at the end of surgery in the control group was compared with measurements at 6 months after surgery. Obtained data were statistically analyzed.There is no statistically significant difference between the 2 groups for growth amount of both sides of the mandible. Difference of ANS-Id (the most anterior points of nasal bone, the most anterior point on the alveolar bone between the lower incisors) and Cd-Id (the uppermost and most distal point of the mandibular condyle, the most anterior point on the alveolar bone between the lower incisors) values of the 2 groups is not statistically significant (P > 0.05).The distance between the 2 screws at the first application significantly increased at the postoperative sixth month (P < 0.05). Metallic fixation of mandibular symphyseal fracture does not affect the vertical and sagittal mandibular growth in growing rabbits.

할로윈 커스튬에 나타난 전통 모티브의 유형 및 상징적 의미

This study examined the characteristics of costumes worn at Halloween parties, a form of cultural entertainment commonly seen in the U.S. The Halloween party culture involves children and adolescents wearing costumes, decorating their bodies, and mimicking famous characters. Furthermore, the types and motifs of Halloween costumes have become a means of expressing individuality. This study conducted empirical research on how such motifs were reflected in modern Halloween party-wear in order to provide basic data regarding the development and design of Halloween costumes and the formation of the related entertainment culture. This study researched the shape of costumes and the type of traditional motif presented in these costumes, as well as analyzed the symbolic meaning of each motif. The research process was as follows. First, this study investigated the origin and evolution of Halloween by researching precedent studies, books, and internet data. Second, this study extracted the types of traditional Halloween motifs by analyzing the characteristics and symbols of Halloween costumes commonly presented in such materials. After collecting 547 Halloween-related images from U.S. and Korean Internet websites, this study sorted and analyzed images that could represent traditional Halloween motifs. According to the results, representative traditional Halloween motifs included black cats, white rabbits, tridents, scarecrows, skull and bones, ghosts, witches, vampires, bats, werewolves, and jack-o-lanterns(pumpkins, etc.). The sentiments for such traditional Halloween motifs changed according to era and race. The symbolic meaning would continuously change - from positive to negative and vice versa - to reflect various socio-cultural phenomena. The results of this study are expected to be used as basic data for developing Halloween costume designs and the related entertainment culture.

ELECTROCONDUCTIBILITY OF POLY N-ISOPROPYLACRYLAMIDE THERMORESPONSIVE HYDROGEL INHIBITS THE OCCURRENCE OF MALIGNANT ARRHYTHMIAS POST MYOCARDIAL INFARCTION

ObjectivesRecentresearch porved the protective effects of synthesizced hydrogels (Gels) formyocardial infarction (MI) animals due to the inhibition of ventricularremodeling. However, cardiac malignant arrhythmia is one of the most seriouscomplications after...

Greatest Evolvability: A Place of Chaos, White Rabbits, Fear, Receptivity and Invention

Based on interviews with successful writers, this essay introduces the concept of greatest evolvability – an intellectual place of uncertainty writers purposefully approach to accelerate the invention process. Aspects of greatest evolvability discussed in finer detail include the edge of chaos, following the white rabbit and fear as indicator. Finally, the receptive stance is offered as a heuristic to transition from fear to invention.

Alpha-lipoic acid reduces peridural fibrosis after laminectomy of lumbar vertebrae in rabbits

Peridural fibrosis is an inevitable healing process causing failed back surgery syndrome after lumbar spinal operations. In this study, alpha-lipoic acid (ALA), reported to reduce fibrosis in liver, oral mucosa, and peritoneum, investigated as a potential candidate for prevention of peridural fibrosis. Twelve adult New Zealand white male rabbits were divided into control (n = 5) and ALA groups (n = 7). Laminectomy of lumbar spine was performed and ALA was applied on the exposed dura mater topically in ALA group. According to histological peridural grading, the ALA group (median grade 1) showed significantly less peridural fibrosis than the control group (median grade 3, p = 0.005). ALA is a promising substance in the prevention of peridural fibrosis, especially in early preoperative and postoperative period.

I Hate to Read! by R. Marshall

Marshall, Rita, and Etienne Delessert. I Hate to Read! Mankato, MN: Creative Education, 1992. Print. As you might guess from the title, this book invites reluctant readers to discover the wondrous adventures that can be accessed through turning the pages of a book, as opposed to tuning into TV programs. The cover features the narrator—third-grader Victor Dickens—on a dragon’s back, with flames licking out of the pages of a stack of books. Readers are told that Victor is a “really good kid” “most of the time,” and many children will be able to relate to his academic difficulties: “Victor got As in math and Bs in science, but Fs when it came to the ABCs”. He calls himself a “victim of the hate to read syndrome”. One night, Victor is visited by a series of storybook creatures, including a crocodile in a white coat, a field mouse carrying gold coins, a pirate parrot, and a white rabbit in black barn boots; all of whom attempt to convince him to read. He repeats his usual mantra, “I hate to read,” until gradually he begins turning pages, and his imagination takes over. Fictional characters morph into people from his real life: Sleeping Beauty turns into Natalie Nickerson, on whom Victor appears to have a bit of a crush. The turning point comes when one of the characters whispers: “It’s fun to read even when you’re not supposed to.” This thought appeals to his sense of juvenile rebellion, and he continues in his literary reverie, even missing his favourite TV program. He imagines his teacher as a witch, throwing books into a cauldron, and his classmates, led by Natalie, shouting, “We hate to read!” Instead of joining in, however, Victor thinks of how sad he would be to lose all of his new friends, so he looks for them in his book: “And as he read each page, he just hated…to come to the end.” Writer and designer Rita Marshall and illustrator Etienne Delessert have each been awarded numerous literary prizes for their work. The large-scale drawings of the storybook creatures are whimsical and inviting. They also contain small details that might not be noticed on the first reading. For example, the toes and talons of the dragon on the cover also form crow-like birds with pointy beaks. In another image, letters of the alphabet are hidden in animal tracks. On the other hand, the book includes some literary references that would likely be lost on a typical third-grader. For example, Victor’s parents are said to have bought all 56 volumes of the Encyclopedia Britannica to help pique his interest in reading. It is unlikely that many families would have this set in their homes, nor would a child normally encounter one in an elementary school library. However, these details will perhaps prompt questions from curious readers. On the whole, “I hate to read” is highly likely to achieve its principal goal. Much like Victor, most children will probably hate to see it come to an end. Not to fear, they can continue to follow Victor’s adventures in “I Still Hate to Read,” which was published in 2007. Highly recommended: 4 out of 4 starsReviewer: Kim Frail Kim is a Public Services Librarian at the H.T. Coutts Education Library at the University of Alberta. Children’s literature is a big part of her world at work and at home. She also enjoys gardening, renovating and keeping up with her two-year old.

Abstract 5462: Single dose dermal irritation study of apaziquone in rabbits

Abstract Introduction and Objectives: Apaziquone is a novel bioreductive alkylating agent in development for intravesical therapy of nonmuscle invasive bladder cancer (NMIBC). The standard dose of apaziquone in all ongoing clinical studies is 4 mg/ 40 mL (0.1mg/mL). For clinical use, lyophilized apaziquone is reconstituted with diluent and instilled in the bladder. During the preparation and handling of the product, there is a possibility of accidental dermal contact for the healthcare professionals. To date, dermal irritation potential of apaziquone has not been investigated. The purpose of this study was to evaluate the dermal irritation potential of apaziquone in rabbits. Materials &amp; Methods: Apaziquone was administered topically on the skin (non-occlusive) to New Zealand White (NZW) rabbits as a single dose for 4 hours followed by removal of excess material, and skin examination for up to 72 hours after removal of excess material. Each male NZW rabbits (3/group; one group) had three identified treatment sites on the back: (1) untreated (2) vehicle (diluent for apaziquone) and (3) apaziquone (0.1 mg/mL). Animals were treated with a single non-occlusive dermal dose of apaziquone (0.5 mL of 0.1 mg/mL) and vehicle for 4 hours followed by removal of excess material. No test material or vehicle was applied to the site designated as untreated. The treatment sites were observed and scored at baseline and at various times (4, 24, 48, and 72 hours) after removal of excess test article and vehicle. The following parameters were evaluated: viability, clinical observations, and dermal observations/scoring by modified Draize method. Results: No clinical signs of toxicity were observed during the study and no signs of dermal irritation related to test article or vehicle/diluent were observed at any of the dose sites in any of animals. The Primary Dermal Irritation Index for apaziquone, administered at clinically relevant exposures, had a value of zero (0) indicating no irritation at the concentration and for the duration of exposure. Conclusion: Apaziquone did not cause any dermal irritation in rabbits at a clinically relevant concentration. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5462. doi:1538-7445.AM2012-5462