White Grain Research Articles

DETERMINATION OF THE AMOUNT OF ZINC IN PURE LINE MAIZE AND SEASONAL VARIATIONS IN GRAINS AND SILKS GROWN ACROSS NIGERIA.

Approximately, 17 percent of the world's population is at risk of zinc (Zn) deficiency. According to literature maize grains and silks are rich in Zn, and the amount varies reflecting different varieties and ecosystems. The amount of Zn in some pure line maize and seasonal variations in grains and silks grown across Nigeria's six geopolitical and seven vegetation zones were determined using acid digestion, and analysis by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). The amount of Zn in the pure line (22.40 mg kg- 1 to 22.46 mg kg-1) and the open-pollinated (22.39 mg kg-1 to 22.48 mg kg-1) maize grains are within the same range, likewise, the white and yellow maize silks (27.99 mg kg-1 to 28.10 mg kg-1) but are higher than the amount in their respective grains. The Mann-Whitney U test at 0.05 significant level was used to evaluate the seasonal variation of Zn. There is no significant difference between the amount of Zn in the white maize grains, likewise, the white and yellow maize silks grown in the dry and rainy seasons across Nigeria's geopolitical and vegetation zones. Except in the South-East where the amount of Zn in the yellow maize grains is significantly higher in the dry season compared with the rainy season. Potentially, 0.67 kg, 0.54 kg, 0.45 kg, and 0.22 kg of the maize grains, and 0.54 kg, 0.43 kg, 0.36 kg, and 0.18 kg silks are recommended for Zn daily dietary intake for men, females, adolescents, and formula-fed babies, respectively.

Grain yellowness is an effective predictor of carotenoid content in global sorghum populations

Identification of high carotenoid germplasm is crucial to assist breeders in provitamin-A biofortification of sorghum (Sorghum bicolor [L.] Moench). High-performance liquid chromatography is the gold standard for carotenoid quantification, however, it is not feasible for large scale phenotyping due to its high cost and low throughput. In this study, we tested the feasibility of using grain color as a high-throughput method of carotenoid biofortification breeding. We hypothesized that visual, color-based selection can be an effective strategy to identify high-carotenoid accessions. Yellow grain had significantly higher carotenoid content than red, brown, and white grain. The degree of yellowness could distinguish the presence or absence of carotenoids, but could not distinguish carotenoid concentrations within yellow-only accessions. The degree of luminosity of the grain, however, was able to better predict carotenoid concentrations within yellow-only accessions. Genome-wide association studies identified significant marker-trait associations for qualitative and quantitative grain color traits and carotenoid concentrations near carotenoid pathway genes—ZEP, PDS, CYP97A, NCED, CCD, and LycE—three of which were common between grain color and carotenoid traits. These findings suggest that using grain color as a method for screening germplasm may be an effective high-throughput selection tool for prebreeding and early-stage breeding in carotenoid biofortification.

Changes in Nutritional and Techno-Functional Properties of Whole Grain Maize Flours Induced by Dry-Heat Treatment.

The present study was carried out to demonstrate the effects of dry heat treatment (DHT) at different temperatures (100, 125, 135, 150, and 165 °C) on the nutritional and techno-functional properties of white, blue, and yellow whole grain maize flour. Results showed that DHT increased the insoluble dietary fiber and free phenolic compounds of the investigated maize flours, while the bound phenolic compounds, anthocyanins, and pasting properties decreased with the rising of the applied temperature. The application of DHT caused the most notable changes regarding the amount of dietary fiber. Content of NDF (neutral detergent fiber) ranged from 11.48% to 44.35%, 14.19% to 37.84%, and 15.15% to 45.86% in white, yellow, and blue maize samples, respectively. Furthermore, at the highest temperature applied in the DHT (165 °C) the content of soluble free phenolic compounds in yellow and blue maize flour samples was 1.2- and 1.4-fold higher compared to control flour samples. DHT significantly improved the functionality of maize flour in terms of water absorption capacity, water solubility, and digestibility, thus it can be effectively used to make up for the poor functionality of raw maize flour. This study shows that DHT at moderate temperatures (125-135 °C), could be a viable solution for the pre-processing of maize flour to enhance the potential for its utilization in the food industry.

EFFECT OF THREE QUINOA VARIETIES AND EDIBLE FLOWERS ON ANTIOXIDANT PROPERTIES OF ALE BEER

Fermentation experiments were conducted with a top-fermenting brewing yeast strain and wort enriched with three varieties of quinoa (with white, red and black grain color), as well as edible flowers (marigold and dandelion) to study their influence on the antioxidant properties of ale beer. Plant additives led to a slight increase in pH and a lower alcohol content of the beer. By itself, quinoa did not improve the functional properties of the beverage, but the combination of black quinoa and edible flowers resulted in an increased content of polyphenols in beer; marigold showed a 48.7 % increase in this parameter compared to the control, and dandelion - 51.2 %. The content of flavonoids in beer was not affected by the addition of plant additives to the wort. A slight increase in anthocyanins (0.7 - 4.4 %) was reported for black quinoa beer as well as black quinoa with flowers. A significant increase in antioxidant activity was reported for the variants with flowers (54 % for marigold and 56.6 % for dandelion). The increased antioxidant capacity combined with the reduced alcohol content of the beers with black quinoa and flowers suggests that a beverage with improved functional properties was obtained. Black quinoa, combined with edible flowers, was more favorable for increasing the antioxidant capacity of beer than other pseudo-cereals (amaranth and chia).

Understanding the molecular regulation of flavonoid 3’-hydroxylase in anthocyanin synthesis: insights from purple qingke

BackgroundThe Flavonoid 3’-hydroxylase gene(F3’H) is an important structural gene in the anthocyanin synthesis pathway of plants, which has been proven to be involved in the color formation of organs such as leaves, flowers, and fruits in many plants. However, the mechanism and function in barley are still unclear.ResultsIn order to explore the molecular mechanism of the grain color formation of purple qingke, we used the cultivated qingke variety Nierumzha (purple grain) and the selected qingke variety Kunlun 10 (white grain) to conduct transcriptomic sequencing at the early milk, late milk and soft dough stage. Weighted Gene Co-expression Network Analysis (WGCNA) was used to construct weighted gene co-expression network related to grain color formation, and three key modules (brown, yellow, and turquoise modules) related to purple grain of qingke were selected. F3’H (HORVU1Hr1G094880) was selected from the hub gene of the module for the yeast library, yeast two-hybrid (Y2H), subcellular localization and other studies. It was found that in purple qingke, HvnF3’H mainly distributed in the cytoplasm and cell membrane and interacted with several stress proteins such as methyltransferase protein and zinc finger protein.ConclusionsThe results of this study provide reference for the regulation mechanism of anthocyanin-related genes in purple grain qingke.

A Wheat Cultivar, “Joongmo2015” with Good Noodles Quality, White Grain Wheat, Higher Winter Hardiness and Adaptable Paddy Culture

A Wheat Cultivar, “Joongmo2015” with Good Noodles Quality, White Grain Wheat, Higher Winter Hardiness and Adaptable Paddy Culture

The utilization of Tepache Probiotic Drink for health benefits and creating household-scale economic opportunities

Probiotic drinks are needed to enrich the microbiome in the digestive organs. Increasing age causes a decrease in the ability of cells to metabolize food, resulting in various degenerative diseases. Tepache probiotic drinks, made from pineapple skin, contain magnesium minerals for bone health, vitamins A, B, and C. Through fermentation, it is enriched by lactic acid bacteria which play a role in helping food metabolism in the intestines and increasing the immune response. Counseling on making tepache probiotic drinks for Family Empowerment and Welfare (PKK) mothers RW 11 Pamulang Timur is expected to improve public health and stimulate the household economy. The method of implementing community service is carried out by means of socialization of the benefits of probiotic drinks, training in making tepache probiotic drinks, financial management and marketing training, and production assistance. Tepache drinks are made from pineapple skin fermented for 24 hours using brown sugar, water, cloves, and cinnamon. The success of fermentation is indicated by the presence of white grains on the surface of the liquid. The fermentation results are filtered and can be consumed directly. The results of the counseling produced a tepache drink product that has been routinely consumed by PKK mothers RW 11 Pamulang Timur, and has an impact on reducing knee pain, tension, blood sugar, uric acid, and cholesterol. The results of household-scale sales have not shown significant profits, but simple calculations if pursued will increase household income.

Investigating the regulatory role of HvANT2 in anthocyanin biosynthesis through protein-motif interaction in Qingke.

Currently, there are no reports on the HvbHLH gene family in the recent barley genome (Morex_V3). Furthermore, the structural genes related to anthocyanin synthesis that interact with HvANT2 have yet to be fully identified. In this study, a bioinformatics approach was used to systematically analyze the HvbHLH gene family. The expression of this gene family was analyzed through RNA sequencing (RNA-seq), and the gene with the most significant expression level, HvANT2, was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in different tissues of two differently colored varieties. Finally, structural genes related to anthocyanin synthesis and their interactions with HvANT2 were verified using a yeast one-hybrid (Y1H) assay. The study identified 161 bHLH genes, designated as HvbHLH1 to HvbHLH161, from the most recent barley genome available. Evolutionary tree analysis categorized barley bHLH TFs into 21 subfamilies, demonstrating a pronounced similarity to rice and maize. Through RNA-Seq analysis of purple and white grain Qingke, we discovered a significant transcription factor (TF), HvANT2 (HvbHLH78), associated with anthocyanin biosynthesis. Subsequently, HvANT2 protein-motifs interaction assays revealed 41 interacting motifs, three of which were validated through Y1H experiments. These validated motifs were found in the promoter regions of key structural genes (CHI, F3'H, and GT) integral to the anthocyanin synthesis pathway. These findings provide substantial evidence for the pivotal role of HvANT2 TF in anthocyanin biosynthesis.

AMARANTH: PROBLEMS AND PERSPECTIVES OF PROCESSING

Amaranth is one of the ancient, so-called pseudo-cereal crops, the first mentions of it date back more than 8,000 years. Despite the sufficiently high study of amaranth and its wide distribution, according to the volume of its cultivation, it can be classified either as a niche crop or as a special purpose crop. Amaranth can be attributed to small-seeded grain crops, the grain has a rounded lenticular, oval-rounded shape with a diameter of 0.9 to 1.7 mm, the weight of 1000 grains is in the range of 0.6 to 1.1 g. Amaranth grains can be white, red, golden, black, or brown in color, with the white grain having the highest manufacturability. In Ukraine, up to 50 enterprises are engaged in the processing of amaranth, while receiving oil, cereals, flakes and flour, but at the moment, despite the high usefulness of these products, a permanent circle of its consumers has not yet been formed in our country, which in turn affects the volume of amaranth cultivation and volumes of its processing into food products. 15 varieties of amaranth are included in the register of plant varieties suitable for distribution on the territory of Ukraine, among which seven varieties are intended for grain. Sterkh, the forage amaranth variety, was the first to enter the Register... in 1994. In the following year, 1998, three amaranth varieties Atstek and Ul'tra and Kremovyi rannii were registered. It should be noted that the first two varieties are grain requirements according to the recommendations of the Register..., and the Kremovyi rannii variety is a fodder variety. In 1999, two more grain varieties of amaranth, Orkhideia and Polischuk, were registered. In 2000, the grain requirements variety Zhaivir was registered. In 2003, the Liera and Sem grain requirements varieties were registered, and in 2009, the Students'kyi variety. Indirectly, amaranth can be characterized by a mass fraction of protein up to 19%, fat up to 9%, carbohydrates up to 60 %, fiber up to 4 %, ash up to 3 %. An impeding factor in increasing the volume of amaranth grain processing is the lack of approved regulations and the lack of enterprises with the appropriate understanding of how to effectively clean amaranth grain from impurities, carry out dehulling, sorting of dehulling products and, most importantly, what kind of product range to produce. All this stops the wide spread of this crop in our country and, accordingly, amaranth products are not widely distributed compared to other traditional crops and are not well known to the domestic consumer.

Adaptation of Conventional Wheat Flour Mill to Refine Sorghum, Corn, and Cowpea

This study evaluated the refinement of sorghum, corn, and cowpea grains using the processing steps and equipment originally designed for wheat milling that consists of a conventional gradual reduction system. The need to mill these grains resulted from a desire to produce alternative ingredients for developing new fortified blended extruded foods used for food aid programming. Milling of white sorghum grain resulted in a crude protein content of 7.4% (wb) for both whole and coarse-milled flour. The crude protein content in whole fine-milled sorghum was 6.8% (wb), which was significantly lower than that of whole coarse flour at 9.3% (wb). A decrease in the ash content of sorghum flour correlates with the decortication process. However, degermed corn, fine and coarse, had significantly different crude protein content of 6.0 ± 0.2% (wb) and 7.7 ± 0.06% (wb), respectively. Degerming of corn improved the quality of corn flour (fine and coarse) by reducing the crude fat content from 3.3 ± 0.18% (wb) to 1.2 ± 0.02% (wb) and 0.6 ± 0.13% (wb), respectively. This helped increase the starch content from 60.1 ± 0.28% (wb) in raw corn to 74.7 ± 0.93% (wb) and 71.8 ± 0.00% (wb) in degermed fine and coarse corn flour, respectively. Cowpea milling did not produce differences in the milling stream outputs when the crude fat and crude protein were compared. Whole flour from the grains had higher milling yields than decorticated flour. This study demonstrated that a mill dedicated to wheat size reduction can be adapted to refine other grains to high quality.

Current challenges and forecasts in maize grain production and consumption in Mexico

Objective: to analyze production and consumption of maize grain in Mexico, with time series and recurrent neural networks, to describe the present and future situation of maize cultivation. Design/ Methodology/ Approach: key variables were analyzed in graphs and maps created in Excel® and SCImago Graphica®, respectively. Forecasts for the year 2050 were obtained in Python© with Recurrent Neural Network (RNN) of the Long Short-Term Memory (LSTM) type, and were compared with the years 1980 and 2020. Results: the largest production of white and yellow maize grain was obtained by the United States and China. Mexico ranks seventh, is not competitive in exports, and relies on imports of yellow maize grain from the United States to supply demand. The Mexican states that implemented technology packages showed higher yields and production. By 2050, maize grain production in Mexico will increase due to the technological advances of Agriculture 5.0 Although it would not be enough to supply the apparent consumption of the growing population, for this reason imports will increase. Limitations/ Implications of the study: analysis of the possible future, created from time series through RNN-LSTM, helps to guide decision-making in the present. Findings/ Conclusions: new agricultural public policies are needed to guide, in the long term, the challenges of maize grain production and consumption in Mexico to guarantee food sovereignty.

Mobile phones and Mozambique farmers: Less asymmetric information and more trader competition?

We measure how the introduction of mobile phones in Mozambique affected unit values of household maize sales, maize producer prices and traders’ margins. Our estimations are based on both representative household surveys for the years 2002, 2003, 2005, 2006 and 2008, and weekly producer and market prices of white maize grain from July 1997 to December 2009 for 15 major producer markets. We find household sales unit values and producer price decreases, and traders’ margin increases between 14% and 22%, indicating benefits for traders. Next, we investigate heterogeneity of impacts in order to explore underlying mechanisms: impacts on margins decrease with population density and network density, and increase with gains from long-distance trade. Impacts on household selling prices are not significantly correlated with educational attainment, but the fall in prices is less with larger household wealth. Impacts on household selling prices and producer prices also become less negative over time, suggesting delayed adoption by farmers. Our results are robust to various threats.

Farmers’ production constraints, preferred varietal traits and perceptions on sorghum grain mold in Senegal

Improving sorghum adoption rates by developing adapted varieties that meet end-user preferences is a major challenge in West Africa. In this study, a participatory rural appraisal was undertaken to identify the main sorghum production constraints, farmers’ preferred variety traits and their perceptions on sorghum grain mold. The study was conducted in four representative rural communities located in the main sorghum producing area of Senegal. A total of 260 farmers were interviewed and data were collected through focus group discussions and individual questionnaires. Our results indicated that Striga, insects, poor soil fertility and drought are the major sorghum producing constraints in Senegal. Grain mold was identified as the second most important sorghum disease after the damping-off. Discoloration on grain surface was the most important criteria farmers used to recognize the disease. The most important sorghum traits farmers desired in improved varieties are medium to short plant maturity cycle, medium plant height, large open or semi-compact panicle, big and white grain, and adaptation to local growing conditions. The results showed that the sorghum cropping system is dominated by male farmers who mainly grow local landraces. These results will provide updated recommendations for the breeding products profile to meet end-user preferences in Senegal.

Association between Fusarium head blight resistance and grain colour in wheat (Triticum aestivum L.)

AbstractThe global yield of wheat is limited by Fusarium head blight (FHB), the most damaging disease of wheat accompanied by mycotoxin contamination. Use of resistant cultivars, from an economical point of view, is the most effective control method for plant diseases. Many naturally occurring secondary metabolites in plants are involved in resistance mechanisms against FHB, especially phenolic compounds with antioxidant properties which caused various colouration of the grain in wheat. The objective of this paper was to evaluate the resistance of wheat with different grain colour on the base of accumulation of deoxynivalenol (DON) in grain and other important FHB traits after inoculation with Fusarium culmorum. Visual symptom score (VSS), Fusarium damaged kernels (FDK) and reduction of grain weight per spike (GWS-R) were determined. This study compared current conventional red wheat cultivars and coloured-grain wheat cultivars or lines with blue aleurone, purple pericarp, red grain and white grain. It was found that the cultivars with a purple pericarp (e.g. Rufia) had the lowest DON content and FDK. Statistically significant interactions between grain colour and year were found for all the variables: DON, VSS, FDK, GWS-R. Red grain materials had the lowest DON levels of all the groups studied in 2016 and 2017, but not in 2018. The most constant and second lowest DON levels in all three years were found in the cultivars/lines with purple pericarp.

Water productivity at two sowing dates of simple white grain maize hybrids

In Mexico, maize stands out for its economic, food and cultural importance. Since ancient times, attempts have been made to increase maize grain yields with different alternatives, including hybridization. Currently, maize hybridization offers great advantages since from the first simple hybrids it is possible to evaluate important variables such as water productivity. The present work was carried out under field conditions in southern Sonora, in two sowing seasons: December 2022- May 2023 (SD 1) and April-August 2023 (SD 2). The research aimed to evaluate the effect of two sowing dates (SD) on phenology, water requirements, water productivity and grain yield. Three single white grain hybrids (L5 X L7; L1 X L2; L2 X L7) obtained from a dialelic design were evaluated. Phenophase occurrence time, irrigation time and interval, total water volume, evapotranspiration, grain yield and water productivity were the evaluated variables. The results showed that in SD 2 significantly reduced the time of occurrence of phenophases in all hybrids due to the effect of temperature. Irrigation time was significantly shorter in SD 2 compared to SD 1. Similarly, the irrigation interval decreased in SD 2 compared to SD 1 with a significant increase in the irrigation lamina. It was necessary to apply two more irrigations to achieve grain filling. Water productivity showed highly significant differences between hybrids and between sowing dates. The simple hybrid L5 X L7 showed better agronomic performance on both sowing dates, demonstrating tolerance to the increase in temperature on SD 2.

Malted sorghum as a maize substitute in broiler diets: effect on feed utilisation, growth performance and haemo-biochemical parameters

Sorghum is amongst the most drought-tolerant cereals in the driest rain-fed regions of the tropics and its utilisation in sustainable poultry production is limited by presence of phytochemicals. Thus, this study evaluated the effects of malting dietary sorghum on growth performance and haemo-biochemical parameters of Ross 308 broiler chickens. A total of 150 unsexed, 2 weeks-old chicks were evenly distributed into 15 replicated pens which were randomly allocated into three experimental diets and fed in two phase feeding. Three diets were formulated by completely replacing maize grain in commercial grower and finisher diets (CON) with red malted sorghum (RSD) and white malted sorghum (WSD). Broilers fed CON and WSD diets gained more weight compared to those fed RSD in week 3 and 5. The lowest feed conversion ratio (FCR) was observed in CON in week 4 and 6. The protein efficiency ratio was the lowest with broilers fed malted sorghum-based diets (MSD) through out the study. Protein intake was the highest in broilers fed WSD in week 3 and 5, but lowest in week 4 and 5 in broilers fed RSD. Diets affected white blood cells basophils, corpuscular (volume and haemoglobin), corpuscular haemoglobin concentration, creatinine, bilirubin, and enzymes (GTP and GOT). Overall, the results from the present study suggest that maize grains can be replaced with malted white sorghum grains in poultry diets, without any adverse effects on the birds’ growth performance and general health status.

Germination: A Powerful Way to Improve the Nutritional, Functional, and Molecular Properties of White- and Red-Colored Sorghum Grains.

This study explored the effects of the germination of red and white sorghum grains (Sorghum bicolor [Moench (L.)]) for up to seven days on various properties of the grain. Germination enriched sorghum's nutritional and sensory qualities while mitigating existing anti-nutritional factors. The study employed Fourier-transformed infrared spectroscopy (FT-IR) and scanning electron microscopy techniques to support its findings. Germination increased protein and lipid content but decreased starch content. White sorghum grains showed elevated calcium and magnesium but decreased iron, potassium, and zinc. Red sorghum grains showed a consistent decrease in mineral content during germination. Germination also increased fiber and lignin values in both sorghum varieties. The results of the FT-IR analysis demonstrate that germination induced significant changes in the molecular structure of white sorghum samples after 24 h, whereas this transformation was observed in red sorghum samples at four days. Total phenolic content (TPC) in red sorghum ranged from 136.64 ± 3.76 mg GAE/100 g to 379.5 ± 6.92 mg GAE/100 g. After 72 h of germination, the germinated seeds showed a threefold increase in TPC when compared to ungerminated seeds. Similarly, the TPC of white sorghum significantly increased (p < 0.05) from 52.84 ± 3.31 mg GAE/100 g to 151.76 mg GAE/100 g. Overall, during the 7-day germination period, all parameters showed an increase, and the germination process positively impacted the functional properties that contributed to the health benefits of white and red sorghum samples.

Tamyb10-D1 restores red grain color and increases grain dormancy via suppressing expression of TaLTP2.128, non-specific lipid transfer protein in wheat

Grain dormancy of wheat is closely associated with grain color: red-grained lines show higher dormancy than white-grained lines. The production of red pigments is regulated by R-1, Tamyb10 gene. However, the relation between grain color and dormancy remains unknown. For this study, we generated transgenic lines which were introduced a DNA fragment containing Tamyb10-D1 gene and its a 2 kb promoter including the 5′ untranslated region into white-grained wheat. Transgenic lines showed red-grained and higher dormant traits. Contents of plant hormones and gene expression of embryos at 30 days after pollination were examined in a wild type and a transgenic line. No differences were observed in the contents of plant hormones, but several genes are differentially expressed between these lines. One differentially expressed gene, TaLTP2.128, is a member of non-specific lipid transfer proteins. It was expressed higher in white grains than in red grains. A putative amino acid sequence showed similarity to that of OsHyPRP5, which is identified as QTL controlling low-temperature germinability in rice. Expression of TaLTP2.128 was increased by grain imbibition. The increasing levels were higher not only in other white-grained lines, but also in non-dormant red-grained lines. TaLTP2.128 was expressed at a quite early stage of germination. These study findings indicate that Tamyb10 regulates dormancy release by the modification of TaLTP2.128 acting as trigger of germination.

Polyphenol oxidase genes in barley (Hordeum vulgare L.): functional activity with respect to black grain pigmentation.

Polyphenol oxidase (PPO) is an oxidoreductase. In damaged plant tissues, it catalyzes enzymatic browning by oxidizing o-diphenols to highly reactive o-quinones, which polymerize producing heterogeneous dark polymer melanin. In intact tissues, functions of PPO are not well understood. The aim of the study was to investigate the barley PPO gene family and to reveal the possible involvement of Ppo genes in melanization of barley grain, which is controlled by the Blp1 gene. Based on known barley Ppo genes on chromosome 2H (Ppo1 and Ppo2), two additional genes-Ppo3 and Ppo4-were found on chromosomes 3H and 4H, respectively. These genes have one and two exons, respectively, contain a conserved tyrosinase domain and are thought to be functional. Comparative transcriptional analyzes of the genes in samples of developing grains (combined hulls and pericarp tissues) were conducted in two barley lines differing by melanin pigmentation. The genes were found to be transcribed with increasing intensity (while grains mature) independently from the grain color, except for Ppo2, which is transcribed only in black-grained line i:BwBlp1 accumulating melanin in grains. Analysis of this gene's expression in detached hulls and pericarps showed its elevated transcription in both tissues in comparison with yellow ones, while it was significantly higher in hulls than in pericarp. Segregation analysis in two F2 populations obtained based on barley genotypes carrying dominant Blp1 and recessive ppo1 (I) and dominant Blp1 and recessive ppo1 and ppo2 (II) was carried out. In population I, only two phenotypic classes corresponding to parental black and white ones were observed; the segregation ratio was 3 black to 1 white, corresponding to monogenic. In population II, aside from descendants with black and white grains, hybrids with a gray phenotype - light hulls and dark pericarp - were observed; the segregation ratio was 9 black to 3 gray to 4 white, corresponding to the epistatic interaction of two genes. Most hybrids with the gray phenotype carry dominant Blp1 and a homozygous recessive allele of Ppo2. Based on transcription and segregation assays one may conclude involvement of Ppo2 but not Ppo1 in melanin formation in barley hulls.

Effective protocol for rice phenotyping based on grain quality: Ranking of rice samples from a segregating BC2 population

The physical properties of rice backcross samples were studied in connection with the development of an effective breeding methodology for creating rice genotypes based on an accelerated breeding process. The purpose of the research was to study the traits of size, vitreousity, and fracturing of samples of the segregating population of BC2 reseedings from the 2022 and 2023 yield. As part of the program for developing varieties based on the use of modern post-genomic and cellular technologies, BC2 samples were used; as parent forms – samples of the USI “Collection of Federal Scientific Rice Centre” of various groups according to the shape of the grain. The crop was grown in artificial climate chambers under controlled conditions of light and air temperature and on the growing site of Federal Scientific Rice Centre. In the study, white grain samples from the segregating BC2 population were isolated for large-scale genotyping. The cultivation of parental forms for hybridization and the crossing process were carried out in artificial climate chambers. Hybridizations were performed using pneumocastration, which included cutting off the flower scales by 1/3 and then removing the anthers using a vacuum compressor. White grain BC2 samples were isolated for research. Seeds were sown on the growing area in containers with 6 kg of soil and Pryanishnikov’s mixture as the main fertilizer. After ripening, the seeds were manually harvested. Plant phenotyping of experimental populations was carried out basing on grain quality traits using certified equipment in accordance with the instructions for the devices and GOST standards. For 15 combinations, samples with the best characteristics of grain quality were selected. In connection with the indicators of physical grain quality traits, BC2 samples were classified into three groups with indicators for each trait: high, medium, low.