Clear, quantitative biological standards are needed for stream protection. Three hundred ninety-seven sites in Wisconsin were used to develop and test stream macroinvertebrate indices of biotic integrity (IBI) designed to provide such standards for the upper Midwestern United States. The IBI development approach was unique in 1) selecting base assemblage metrics a priori, 2) using watershed land cover and local habitat to quantify human influence, 3) weighting local- and water- shed-scale variables to characterize human influence in a biologically meaningful way, and 4) weight- ing macroinvertebrate metrics to characterize the assemblage in a way that detects human influence. Canonical correlation analysis provided IBI models by identifying the strongest relation between a set of macroinvertebrate metrics and a set of environmental condition measures. Metrics related to assemblage composition, structure, and function represented the macroinvertebrates, including or- ganic pollution tolerance, sediment tolerance, species richness, proportion or number of Ephemer- optera-Plecoptera-Trichoptera, Amphipoda, Isopoda, Diptera, and Chironomidae taxa, and propor- tion that feed by shredding, scraping, and gathering. Environmental condition measures included proportions of urban, forage crop, row crop, forest, open-water, and wetland land cover at the wa- tershed scale, and riparian condition, bed and bank condition, and habitat heterogeneity at the local scale. The Driftless Area and Northern Forest ecoregions were unique and warranted separate models, whereas the Central and Southeast ecoregions were merged into one model. Partial regressions iden- tified the relative influence of variables at the watershed and local scales. Model testing with inde- pendent data indicated the appropriateness of classification into the 3 regions, and that the IBIs could predict human influence well. The IBI models show promise for land-cover planning, pollution mon- itoring, and developing biological criteria for stream and watershed protection.
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