The seasonal pattern of species diversity and abundance of waterbirds of the Chambal River in the extent of Mukundra Hills National Park, Rajasthan, India was evaluated. The pre-monsoon (March–April 2021) and post-monsoon surveys (August–September 2021) were carried out using the direct count method with the help of a motorboat to monitor the population status of waterbirds. A total of 44 species of waterbirds belonging to 15 families and nine orders were identified, including 11 winter migratory species. Forty species of birds were recorded during pre-monsoon and 27 species during post-monsoon seasons. The species richness and relative abundance varied significantly between observed seasons (x2 = 532.77, df = 43, p <0.05). Breeding activities of three species were recorded, namely, Grey Heron, Black-crowned Night Heron, and the ‘Near Threatened’ Woolly-necked Stork. The present study reveals the status of waterbirds in the protected area of Mukundra Hills National Park.