Water Dispersible Granule Research Articles

Using Pyroxasulfone for Downy Brome (<i>Bromus tectorum</i> L.) Control in Winter Wheat

Downy brome is one of the most troublesome weeds in no-till wheat production systems of the US Great Plains. Pyroxasulfone is a relatively new, soil-applied residual herbicide (root/shoot growth inhibitor) labeled for use in wheat. Multiple field experiments were conducted near Huntley, MT from 2012 through 2016 to determine the efficacy of pyroxasulfone to control downy brome in imidazolinone (IMI)-tolerant (Clearfield&#8482) winter wheat. Pyroxasulfone did not cause any injury to wheat in any of the three studies. Downy brome injury with pyroxasulfone preemergence (PRE) only program did not differ between 89 or 178 g·ai (active ingredient)·ha-1 rates, and averaged 82% and 84% in 2 separate studies. In a preplant (PP) burndown program, the addition of pyroxasulfone (178 g·ai·ha-1) to glyphosate improved downy brome end-season injury from 15% to 74%. In a separate study, the end-season injury with pyroxasulfone was greater when applied PRE (84%) compared to the delayed PRE (DPRE) timing (74%). In addition, the water dispersible granule (WDG) formulation of pyroxasulfone performed slightly better than the suspension concentrate (SC) formulation for downy brome injury. Pyroxasulfone applied PRE in the fall at a rate of 89 g·ai·ha-1 followed by (fb) imazamox (44 g·ai·ha-1 rate) applied postemergence (POST) in the spring effectively controlled downy brome (99% end-season injury). Furthermore, the injury was consistent with the standard program comprising of propoxycarbazone (29 g·ai·ha-1) PRE fb imazamox POST in IMI-tolerant winter wheat. In conclusion, pyroxasulfone applied PRE in the fall can be effectively utilized in conjunction with a standard acetolactate synthase (ALS)-inhibitor-based POST herbicide program for a season-long downy brome management in winter wheat.

Effect of particle size of active ingredient on physicochemical properties of water dispersible granules obtained through direct granulation of agrochemical suspension using fluidized bed

Effect of particle size of active ingredient on physicochemical properties of water dispersible granules obtained through direct granulation of agrochemical suspension using fluidized bed

Simultaneous detection of sulfoxaflor and its metabolites, X11719474 and X11721061, in lettuce using a modified QuEChERS extraction method and liquid chromatography-tandem mass spectrometry.

An analytical method has been developed to quantify the residual levels of sulfoxaflor and its metabolites (X11719474 and X11721061) in/on cultivated lettuce grown under greenhouse conditions. Samples were extracted and purified using a quick, easy, cheap, effective, rugged, and safe 'QuEChERS' method (original version) following systematic method optimization and were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity with coefficient of determination ≥0.9930 was obtained and the limits of detection (LOD) and quantification (LOQ) were in the ranges of 0.003-0.006 and 0.01-0.02 mg/kg, respectively. The recovery rates of both the parent compound and its metabolites (fortified at 10 and 50× the LOQ) estimated from six replicates ranged between 81.9 and 115.5% with a relative standard deviation <12%. The validated method was applied to field-incurred samples (collected over 7 days) sprayed once or twice with a water-dispersible granule formulation. Notably, a substantial reduction in rate was observed after 3 days and the half-life was short, only 1.5 days. The developed method is simple and versatile and can be used for various leafy vegetables.

Bacterial Larvicide, Bacillus thuringiensis israelensis Strain AM 65-52 Water Dispersible Granule Formulation Impacts Both Dengue Vector, Aedes aegypti (L.) Population Density and Disease Transmission in Cambodia.

A multi-phased study was conducted in Cambodia from 2005–2011 to measure the impact of larviciding with the bacterial larvicide, Bacillus thuringiensis israelensis (Bti), a water dispersible granule (WG) formulation on the vector, Aedes aegypti (L.) and the epidemiology. In our studies, all in-use containers were treated at 8 g/1000 L, including smaller containers and animal feeders which were found to contribute 23% of Ae aegypti pupae. The treated waters were subjected to routine water exchange activities. Pupal production was suppressed by an average 91% for 8 weeks. Pupal numbers continued to remain significantly lower than the untreated commune (UTC) for 13 weeks post treatment in the peak dengue vector season (p<0.05). Suppression of pupal production was supported by very low adult numbers in the treated commune. An average 70% of the household harbored 0–5 Ae aegypti mosquitoes per home for 8 weeks post treatment, but in the same period of time >50% of the household in the UTC harbored ≥11 mosquitoes per home. The adult population continued to remain at significantly much lower numbers in the Bti treated commune than in the UTC for 10–12 weeks post treatment (p<0.05). In 2011, a pilot operational program was evaluated in Kandal Province, a temephos resistant site. It was concluded that 2 cycles of Bti treatment in the 6 months monsoon season with complete coverage of the target districts achieved an overall dengue case reduction of 48% in the 6 treated districts compared to the previous year, 2010. Five untreated districts in the same province had an overwhelming increase of 352% of dengue cases during the same period of time. The larvicide efficacy, treatment of all in-use containers at the start of the monsoon season, together with treatment coverage of entire districts interrupted disease transmission in the temephos resistant province.

Field evaluation and determination of four herbicides in a wheat ecosystem by a simple and versatile QuEChERS method with liquid chromatography-tandem mass spectrometry

ABSTRACTWheat products are consumed worldwide and every day; therefore, herbicides misuse in the wheat ecosystem has become a global food safety issue. Here, a simple and versatile method, QuEChERS (quick, easy, cheap, effective, rugged, and safe) with liquid chromatography-tandem mass spectrometry was developed to measure four herbicides in soil, wheat, and wheat straw – florasulam, carfentrazone-ethyl, fluroxypyr-meptyl, and fluroxypyr. By adjusting the amount of graphitized carbon black from 0 to 10 mg, the herbicides could be extracted with satisfactory recoveries of 80%–110%. Application of two water-dispersible granules showed first-order kinetics as well as half-lives between 1.2 and 5.1 d under open-field conditions. The dissipation kinetics of the four herbicides differed in the soil, wheat, and wheat straw, with residual concentrations in straw at 2 h after application being higher than in soil and faster degradation occurring in straw. The terminal residues in soil, wheat grain, and wheat straw were all below the maximum residue limits. The developed method was easy to handle and versatile; thus, it will facilitate regulation and inspection for possible misuse against Good Agriculture Practices. Moreover, the results of this study will contribute to global environmental protection as well as food safety issues.

Evaluation of different formulations of IGRs against Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae)

ObjectiveTo test the relative efficacy of pyriproxyfen and methoprene on mortality, deformity, inhibition and emergence to adult stages of Culex quinquefasciatus and Aedes albopictus. MethodsSerial dilutions (0.01–0.05 mg/L) of methoprene, pyriproxyfen 0.5 water dispersible granules (WDG) and pyriproxyfen 1.0 WDG were used to assess mortality and inhibition of 3rd instar larvae of Aedes albopictus and Culex quinquefasciatus. Each concentration and control was replicated four times in completely randomized design. Data on larval mortality, growth inhibition, deformities and adult's emergence was recorded weekly. On the basis of best comparative performance, the efficacy of pyriproxyfen 1.0 WDG at 0.1 g/m3 was also tested in the field by collecting treated water samples monthly for 1–6 months after field application. Twenty five 3rd instar larvae of Aedes and Culex spp. of the same cohorts were used for bioassays and compared with larvae in control cups containing 1 L of untreated tap water. ResultsResults revealed variations in fatality of different insect growth regulators (IGRs) to the 3rd instar larvae of Culex and Aedes mosquitoes. Among the IGRs, pyriproxyfen 1.0 WDG was found best that exhibited significantly high emergence inhibition against Culex and Aedes spp. Based on the results, the IGRs were classified in terms of the tested parameters in order of pyriproxyfen 1.0 WDG > pyriproxyfen 0.5 WDG > methoprene. In case of field studies, pyriproxyfen 1.0 WDG, pool data of the entire target treated sites showed minimum adult emergence from water sampled of habitats treated with 0.1 g/m3 of pyriproxyfen 1.0 WDG. ConclusionsIt is thus concluded that IGRs can be utilized as environment friendly control measures for Culex and Aedes spp. of mosquitoes on small and large scale. This will reduce the use of conventional insecticides by the public health authorities and help in reducing selection pressure of insecticides.

Efficacy of water-dispersible formulations of biological control strains of Aspergillus flavus for aflatoxin management in corn

ABSTRACTField experiments were conducted in 2011 and 2012 to evaluate the efficacy of water-dispersible granule (WDG) formulations of biocontrol strains of Aspergillus flavus in controlling aflatoxin contamination of corn. In 2011, when aflatoxin was present at very high levels, there was no WDG treatment that could provide significant protection against aflatoxin contamination. The following year a new WDG formulation was tested that resulted in 100% reduction in aflatoxin in one field experiment and ≥ 49% reduction in all five WDG treatments with biocontrol strain 21882. Large sampling error, however, limited the resolution of various treatment effects. Corn samples were also subjected to microbial analysis to understand better the mechanisms of successful biocontrol. In the samples examined here, the size of the A. flavus population on the grain was associated with the amount of aflatoxin, but the toxigenic status of that population was a poor predictor of aflatoxin concentration.

Preparation and Evaluation of Emamectin Benzoate Solid Microemulsion

The solid microemulsions of emamectin benzoate with the same content of surfactants were prepared by a self-emulsifying method. Emulsifier 600#and emulsifier 700#(3/2, w/w) screened from eleven kinds of commonly used surfactants displayed great emulsifying properties. The redispersed solution of the solid microemulsion presented aqueous microemulsion characteristic. The mean particle size and polydispersity index were 10.34 ± 0.10 nm and 0.283 ± 0.013, respectively. The solid microemulsion showed excellent storage stability and the bioassay compared with water dispersible granules against diamondback moths provided a proof of its improved biological activities. This formulation could significantly reduce surfactants and is perspective in plant protection for improving bioavailability and environmental friendliness.

시설재배 아스파라거스 중 살충제 acetamiprid의 잔류특성

Abstract This study was carried out to investigate residual characteristics of insecticide acetamiprid inasparagus under greenhouse condition from July to August and consequentially to obtain basic data forguideline on safe use of this pesticide in asparagus. Residues of acetamiprid in young stem of asparagusbefore and after removing foliage were analyzed from samples harvested at 0, 1, 3, 5 and 7 days after singleapplication before harvest. As a result, residues of acetamiprid in young stem of asparagus before and afterremoving foliage at 0 day were 0.27 mg/kg and 0.14 mg/kg, respectively, which were higher than tentativelimit (0.1 mg/kg). However, 3 days later residues of acetamiprid were lower than the tentative limit, representing0.08 mg/kg and 0.03 mg/kg in the asparagus before and after removing foliage, respectively. Acetamiprid wasundetectable in both samples at 5 days since the concentrations were less than detection limit (0.02 mg/kg) inthis study. In summary, the half-life of acetamiprid in asparagus regardless of removing foliage was quiteshort under greenhouse condition from July to August, in the range of 1-3 days, and single application ofacetamiprid water dispersible granule in/on asparagus at 7 days before harvest would have no problem onsafety issues about pesticide residue. This result might be basic information to construct guideline for safeuse of acetamiprid in asparagus.Key words Acetamiprid, Asparagus, Pesticide residues

농작업자의 Kresoxim-methyl과 fenthion에 대한 호흡노출량 측정을 위한 IOM 채집기의 효율성 평가

본 연구는 농작업자의 호흡노출 측정에 사용되어오던 고체흡착제 외에 최근에 사용되는 유리섬유여과지가 장착된 IOM 채집기에 대해서, 고체 제제인 kresoxim-methyl 입상수화제와 액상 제제인 fenthion 유제를 이용하여 포집효율과 파과율을 측정하여 효율성을 검증하고자 하였다. LC-MS/MS 기기상 최소검출량은 12.5 pg, 분석법상 검출한계는 5.0 ng/mL이었고, 각 노출 시료의 matrix matched standard의 직선성은 <TEX>$R^2$</TEX> 값이 0.999 이상이었다. 사용된 유리섬유여과지와 고체흡착제에서의 두 가지 농약의 회수율은 유리섬유여과지는 kresoxim-methyl 102-109%, fenthion 97-104%, XAD-2 resin 고체흡착제는 각각 94-98%, 93-100%이었다. 포집효율은 IOM 채집기(유리섬유여과지 장착)와 고체흡착제(XAD-2 resin)를 연결한 후 개인용 공기펌프에 연결하여 측정하였다. 고체 및 액상 2가지 제제를 표준배율로 희석하여 IOM 채집기에 분무하여 포집효율을 본 결과 두 제제 모두 유리섬유여과지에 포집되었고 고체흡착제로 통과되지 않았다. 파과시험은 IOM 채집기의 유리섬유여과지에 농약 표준 용액을 가하고 고체흡착제를 연결한 후 개인용 공기펌프에 연결하여 측정하였다. 파과시험 결과, kresoxim-methyl은 87-101%, fenthion은 96-105%가 첫번째 유리섬유여과지에 흡착/보유되어 있었고 두 번째 유리섬유여과지나 고체흡착제로 파과되지 않았다. 따라서 유리섬유여과지가 장착된 IOM 채집기는 고체 제제나 액상 제제나 상관없이 포집효율과 흡착/보유 능력이 뛰어난 것으로 검증되어 농약 호흡노출 연구에 적극 활용될 것으로 판단한다. An IOM sampler equipped with glass fiber filter has been recently utilized instead of solid adsorbent, which was used to measure the inhalation exposure of agricultural operator to pesticides. The aim of this study is to validate the efficacy of an IOM sampler by measuring the trapping efficiency and breakthrough using kresoxim-methyl water-dispersible granule and fenthion emulsifiable concentrate. On LC-MS/ MS, minimum detection level was 12.5 pg and method limit of detection was 5.0 ng/mL. Good linearity (<TEX>$R^2$</TEX> > 0.999) for matrix matched standards was obtained. Recoveries of pesticides from glass fiber filter were 102-109% (kresoxim-methyl) and 97-104% (fenthion) while those from XAD-2 resin were 94-98% (kresoxim methyl) and 93-100% (fenthion). Trapping efficiency test was performed with personal air pumps and IOM sampler (glass fiber filter) connected with solid adsorbent (XAD-2 resin) with two types of formulation (solid and liquid) which were diluted by standard rate and sprayed to IOM sampler. Those pesticides were trapped only in glass fiber filter without any breakthrough to solid adsorbent. After spiking of pesticides to glass fiber filter, breakthrough test was carried out with IOM sampler (glass fiber filter) which was connected with solid adsorbent. As a results, 87-101% of kresoxim-methyl and 96-105% of fenthion remained in spiked glass fiber filter, however, no pesticides were detected in second glass fiber filter and solid adsorbent. In conclusion, IOM sampler which equipped with glass fiber filter can be applied widely for pesticide inhalation exposure study since it has good trapping efficiency and adsorption capacity, regardless of the solid or liquid formulation.

Determination and analysis of the dissipation and residue of cyprodinil and fludioxonil in grape and soil using a modified QuEChERS method

A simple and accurate method coupled with a gas chromatography-nitrogen phosphorus detector was developed to detect cyprodinil and fludioxonil in grape and soil. The accuracy and precision of the method in detecting the two fungicides were evaluated by conducting intra- and inter-day recovery experiments. The limits of detection were 0.017 mg/kg for cyprodinil and 0.030 mg/kg for fludioxonil. The limits of quantitation were 0.05 mg/kg for cyprodinil and 0.10 mg/kg for fludioxonil in grape and soil. The recoveries of the fungicides in grape and soil were investigated at three spiked levels and were found to range from 85.81 to 102.94% for cyprodinil and from 92.00 to 106.86% for fludioxonil, with relative standard deviations below 7%. Field experiments were conducted in two experimental locations in China. The half-lives of cyprodinil were 9.6-20.8 days in grape and 5.8-15.6 day in soil, and the half-lives of fludioxonil were 6.2-7.2 days in grape and 6.0-12.1 days in soil. When the cyprodinil and fludioxonil 62% water-dispersible granule formulation was sprayed at a low dosage three times, terminal residues of cyprodinil and fludioxonil were below 1.0 mg/kg in grape 14 days after harvest. This work may serve as a reference to establish the maximum residue limits for cyprodinil and fludioxonil in grape and promote the proper and safe use of these two fungicides.

Method validation and dissipation kinetics of four herbicides in maize and soil using QuEChERS sample preparation and liquid chromatography tandem mass spectrometry

A versatile liquid chromatography tandem mass spectrometry method with modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation was developed for the determination of rimsulfuron, mesotrione, fluroxypyr-meptyl, and fluroxypyr. By adjusting the amount of graphitized carbon black, the herbicide analytes could be quantified with satisfactory recoveries in the range of 80–110%. A dissipation kinetics study conducted under open field conditions at two sites during 2014 showed first order equations with half-lives between 0.6d and 3.6d, illustrating an appropriate degree of stability and safety. The dissipation kinetics were different in the different matrices. Although the herbicides had higher initial residues in straw than those in soil, they degraded faster in straw. The terminal residues for the herbicides formulated in two water dispersible granules were all below maximum residue limits. These results not only gave insights about the analytes but also contributed to environmental protection and food safety.

Dissipation, residues, and safety evaluation of trifloxystrobin and tebuconazole on ginseng and soil.

Supervised field trials at two locations in 2012 and 2013 were conducted to evaluate the dissipation, terminal residues, and safety evaluation of Nativo 75 water dispersible granule (WG) (25 % trifloxystrobin + 50 % tebuconazole) on ginseng and soil following foliar application at a recommended dose 150 (50 + 100) and 1.5 times of the recommended dosage 225 (75 + 150) g a.i. ha(-1). The average recoveries of trifloxystrobin and tebuconazole at three spiking levels in ginseng root, stem, and leaf and in soil were in the ranges of 81.0-96.8 % and 80.2-97.5 % with relative standard deviations (RSDs) of 4.92-13.13 % and 4.67-8.35 %, respectively. The half-lives of trifloxystrobin and tebuconazole were 5.92-9.76 days and 4.59-7.53 days, respectively. The terminal residues were all below the maximum residue limits (MRLs) of EU, USA, Canada, Japan, and South Korea. The food safety was evaluated by comparing the estimated daily intake (IEDI) with its acceptable daily intake (ADI). IEDI values calculated from residue data were found to be far less than the ADI on ginseng. Therefore, it would be unlikely to cause health problems induced by Nativo 75 WG use on ginseng at a dosage of 150-225 g a.i. ha(-1).

顆粒水和剤の崩壊性に影響を与える要因に関する研究

顆粒水和剤の崩壊性に影響を与える要因に関する研究

경구흡수율을 반영한 농약의 AOEL 설정 및 농작업자 위해성 평가

Abstract Methods of establishment of AOEL (Acceptable Operator Exposure Level), application of oralabsorption by country, and calculation of exposure dose for operator risk assessment in USA, EU and Koreawere investigated. Oral absorption of 141 active substances for pesticides was also investigated, then operatorrisk assessment was conducted with AOEL including oral absorption and Korean AOEL. Internal doseconverted to external dose with oral or dermal absorption in USA and EU, but external dose to which oralabsorption was not applied was used for establishment of AOEL in Korea. Oral absorption of 50 activesubstances among 141 were below 80%. In case of application of oral absorption as a correction factor inbelow 80%, AOELs of about 36% active substances were considered to be lower than the current KoreanAOELs. Operator risk assessment of 28 active substances among 50 active substances with oral absorptionbelow 80% was conducted with EU AOELs. TER (Toxicity Exposure Ratio) of 12 plant protection productsincluding chlorothalonil WG (Water-dispersible Granule) was less than 1 and the risk was high. Operator riskassessment of 24 active substances among 50 active substances with oral absorption below 80% wasconducted with Korean AOELs. TER of 6 plant protection products including chlorothalonil WG were lessthan 1 and the risk was high. Operator risk assessment of 4 plant protection products not having KoreanAOEL was conducted with converted EU AOEL into AOEL not including oral absorption. The resultsindicated TER of 4 products including daminozide WP (Wettable Powder) was over 1 and risk was low. 22products except 6 products such as oxadiagyl SC (Suspension Concentration) were shown the same results ofrisk assessment between EU AOELs and Korean AOELs. As a result, it was considered that AOELsincluding oral absorption was possible to be used for operator risk assessment. It was considered operator riskassessment with AOEL including oral absorption was more like real assessment method, and improvement ofassessment was needed for application to evaluate pesticides in registration. Key words AOEL, oral absorption, risk assessment

Experimental hut and bioassay evaluation of the residual activity of a polymer-enhanced suspension concentrate (SC-PE) formulation of deltamethrin for IRS use in the control of Anopheles arabiensis.

BackgroundThe Stockholm Convention on Persistent Organic Pollutants (POPs) came into effect in 2004; the use of DDT for malaria control has been allowed to continue under exemption since then due to a perceived absence of equally effective and efficient alternatives. Alternative classes of insecticide for indoor residual spraying (IRS) have a relatively short residual duration of action (2-6 months according to WHO). In areas of year-round transmission multiple spray cycles are required, resulting in significantly higher costs for malaria control programs and user fatigue. This study evaluated performance of a new formulation of deltamethrin (pyrethroid) with polymer (SC-PE) to prolong the effective residual action to >6 months.MethodsDeltamethrin SC-PE was evaluated alongside an existing water dispersible granule (WG) formulation and DDT water dispersible powder (WP) in laboratory and hut bioassays on mud, concrete, palm thatch and plywood substrates. An experimental hut trial was conducted in Lower Moshi Rice Irrigation Zone, Tanzania from 2008-2009 against wild, free-flying, pyrethroid susceptible An. arabiensis. Performance was measured in terms of insecticide-induced mortality, and blood-feeding inhibition. Bioassays were carried out monthly on sprayed substrates to assess residual activity.ResultsBioassays in simple huts (designed for bioassay testing only) and experimental huts (designed for testing free flying mosquitoes) showed evidence that SC-PE improved longevity on mud and concrete over the WG formulation. Both deltamethrin SC-PE and WG outperformed DDT in bioassays on all substrates tested in the laboratory and simple huts. In experimental hut trials SC-PE, WG and DDT produced high levels of An. arabiensis mortality and the treatments were equivalent over nine months’ duration. Marked seasonal changes in mortality were recorded for DDT and deltamethrin treatments, and may have been partly influenced by outdoor temperature affecting indoor resting duration of mosquitoes on sprayed surfaces, although no clear correlation was demonstrated.ConclusionsThere is a limited range of alternative insecticides for IRS, and deltamethrin SC-PE is likely to have an important role as part of a rotation strategy with one or more different insecticide classes rotated annually, particularly in areas that currently have low levels of pyrethroid resistance or low LLIN coverage and year-round malaria transmission.

Effect of two formulations on the decline curves and residue levels of rotenone in cabbage and soil under field conditions

The dissipation and residue levels of rotenone with suspension concentrate (SC) as well as water dispersible granule (WDG) formulations in cabbage and soil were investigated under field conditions. The decline curves of rotenone residues in cabbage and soil corresponded with first-order kinetics. The dissipation rate of rotenone in cabbage was faster than that in soil. The half-lives of the SC formulations in cabbage and soil were 1.14d to 1.85d and 2.26d to 3.98d, respectively. For the WDG formulation, the values were 1.33d to 1.96d and 2.41d to 3.38d. Less spraying, lower dosage, and longer pre-harvest intervals could reduce terminal residue levels. Terminal residues below the MRL (0.5mgkg−1) were achieved after pre-harvest intervals of 3d at either once (45ga.i.ha−1) or twice (90ga.i.ha−1) the recommended dosage. The different dissipation processes of rotenone in cabbage and soil under field conditions could be attributed to the different climate and soil properties, and the types of formulation had no apparent effect on half-life. Formulation type affected the initial concentrations and finally led to different terminal residues. Prolonged pre-harvest intervals are necessary to ensure that the products are sufficiently safe for consumption.

Experimental hut and bioassay evaluation of the residual activity of a polymer-enhanced suspension concentrate (SC-PE) formulation of deltamethrin for IRS use in the control of Anopheles arabiensis

BackgroundThe Stockholm Convention on Persistent Organic Pollutants (POPs) came into effect in 2004; the use of DDT for malaria control has been allowed to continue under exemption since then due to a perceived absence of equally effective and efficient alternatives. Alternative classes of insecticide for indoor residual spraying (IRS) have a relatively short residual duration of action (2-6 months according to WHO). In areas of year-round transmission multiple spray cycles are required, resulting in significantly higher costs for malaria control programs and user fatigue. This study evaluated performance of a new formulation of deltamethrin (pyrethroid) with polymer (SC-PE) to prolong the effective residual action to >6 months.MethodsDeltamethrin SC-PE was evaluated alongside an existing water dispersible granule (WG) formulation and DDT water dispersible powder (WP) in laboratory and hut bioassays on mud, concrete, palm thatch and plywood substrates. An experimental hut trial was conducted in Lower Moshi Rice Irrigation Zone, Tanzania from 2008-2009 against wild, free-flying, pyrethroid susceptible An. arabiensis. Performance was measured in terms of insecticide-induced mortality, and blood-feeding inhibition. Bioassays were carried out monthly on sprayed substrates to assess residual activity.ResultsBioassays in simple huts (designed for bioassay testing only) and experimental huts (designed for testing free flying mosquitoes) showed evidence that SC-PE improved longevity on mud and concrete over the WG formulation. Both deltamethrin SC-PE and WG outperformed DDT in bioassays on all substrates tested in the laboratory and simple huts. In experimental hut trials SC-PE, WG and DDT produced high levels of An. arabiensis mortality and the treatments were equivalent over nine months’ duration. Marked seasonal changes in mortality were recorded for DDT and deltamethrin treatments, and may have been partly influenced by outdoor temperature affecting indoor resting duration of mosquitoes on sprayed surfaces, although no clear correlation was demonstrated.ConclusionsThere is a limited range of alternative insecticides for IRS, and deltamethrin SC-PE is likely to have an important role as part of a rotation strategy with one or more different insecticide classes rotated annually, particularly in areas that currently have low levels of pyrethroid resistance or low LLIN coverage and year-round malaria transmission.

Degradation Dynamics and Residue Analysis of Flubendiamide in Cabbage and Soil by Liquid Chromatography-Tandem Mass Spectrometry with Dispersive Solid Phase Extraction

To formulate a scientific basis for a reasonable spray dose and safe interval period of 20% flubendiamide water dispersible granule (WDG) on controlling vegetable pests, degradation dynamics of flubendiamide in cabbage and soil was analyzed in this study. Dissipation and residue of flubendiamide in 20% flubendiamide WDG in cabbage and soil under field conditions were investigated by liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction. Results showed that the degradation dynamic equations of flubendiamide in cabbage and soil were based on the first-order reaction dynamic equations. The half-lives of the degradation of flubendiamide were 3.51 d to 3.96 d and 3.43 d to 3.87 d in the cabbage of Yangzhou and Jingzhou, respectively, and 4.42 d to 5.13 d and 4.37 d to 4.99 d in the soil of Yangzhou and Jingzhou, respectively. The terminal residues of flubendiamide in the cabbage of Yangzhou and Jingzhou were 0.0247 mg&middotkg-1 to 0.0393 mg&middotkg-1 and 0.0225 mg&middotkg-1 to 0.0273 mg&middotkg-1, respectively, when 20% flubendiamide WDG was applied at a dose of0.050g&middotm-2. Flubendiamide is safe to be applied in cabbage fields at the recommended dose.

Nutrient and water management for higher sugarcane production, better juice quality and maintenance of soil fertility-A review

The application of at least 150 kg N/ha for the plant and 225 kg N/ha for the ratoon crops is required and N should be applied in three equal split one third at planting, one third at about 6–10 weeks after planting and one third at before on set of mousoon. 30th of June. The application three spray of 2.5% urea in May and June and rest N through soil application produced significantly higher cane yield (6.2% and 6.5% for plant and ratoon crops, respectively), yield parameters and CCS% compared to recommended use of N application. The application of phosphorus @ 50 kg P2O5/ha and potash @ 50 kg K2O/ha in both sugarcane plant and ratoon crops showed substantial increase in cane yield and net return over no P and K application respectively. The recommendation of application of 50 kg P2O5/ha and 50 kg K2O/ha in both sugarcane plant and ratoon crops has been included in the package of practices for the farmers of Haryana. Application of Water Dispersible Granules of sulphur @ 18 kg S/ha, 13.5 kg S/ha and gypsum @ 25 kg S/ha. increased 9.6%, 6.5% and 7.7% of cane yield over no S application. Iron deficiency in sugarcane can be corrected with 2–3 spray of 1% FeSO4 at 10–15 days interval. Application of znson 25kg/ha is only recommended for light textured soil. The leaf analysis could be justified as a mean for predicting cane and sugar yield. Integrated use of organic manure (FYM, pressmud and green manuring of dhaincha) with NPK produced significantly higher cane yield of plant and ratoon crops over NPK alone and improved soil fertility. The application of sugarcane trash @ 3t/ha in combination with N significantly increased the organic carbon (OC%), available P and K, infiltration rate and moisture retention in the soil over no trash. The bio-fertilizers application helps in saving of 1/3rd dose of N and also helps in phosphorus application through rock phosphate source. Five sugarcane genotypes were identified as nitrogen (N) efficient genotypes and five sugarcane genotypes were identified as phosphorus (P) efficient genotypes. Amelioration of irrigation water having RSC with gypsum or press mud significantly increased the cane yield of sugarcane. The genotypes CoH97, CoH35, CoH56, CoJ 64, CoH 92 and CoS8436 were sensitive to sodicity whereas genotypes CoH99, CoH 110, CoH 119 and CoS 767 were tolerant. Drip irrigation system in sugarcane showed increase of 15% in cane yield or saving of 2230% irrigation water at the equivalent yield, than continuous furrow irrigation.