Fruit Waste Research Articles

Bioethanol production from pineapple fruit waste juice using bakery yeast

The declining oil production stemming from decreasing raw material reserves has clashes with rising demands and has created a supply-demand gap in the overall energy sector. Excessive consumption of fossil fuel oil exacerbates environmental issues, potentially leading to global climate change and increased natural disasters. Consequently, there are efforts in looking for alternate renewable fuel sources. The study included physical pre-treatment, natural hydrolysis, natural fermentation, fermentation of pineapple waste juice using bakery yeast, and subsequent distillation. The pineapple wastes produced juice with 12.67 °Brix and pH range of 3.16–3.18. The present study reports bioethanol production from pineapple waste mixed with bakery yeast (Saccharomyces cerevisiae) and pineapple wastes juice without bakery yeast, revealing that the yeast-amended mixture yielded bioethanol with alcohol content of 45 % compared to 36 % from pineapple juice alone. Re-distillation enhanced bioethanol content from 25 % - 45 %–85 % which aligns well with E85 fuel specifications, indicating bioethanol's suitability as fuel. Thus, bioethanol derived from pineapple fruit wastes presents a promising renewable energy solution. This study investigates the production of bioethanol from pineapple waste juice by comparing two methods: one using bakery yeast and the other without yeast. Both methods are conducted at room temperature to evaluate their efficiency and effectiveness in converting pineapple waste juice into bioethanol.

Particle Size Effect on Anaerobic Digestion of Fruit and Vegetable Waste

During the anaerobic digestion (AD) of fruit and vegetable waste (FVW), excessive particle size reduction can lead to the overproduction and inhibition of methanogenic microorganisms. This paper presents an in-depth analysis through experimental assays, modeling, and response surface analysis of the effect of particle size on methane production. A simple model was proposed considering the inhibition of the growth of methanogenic microorganisms and surface-based hydrolysis kinetics. The model parameters were estimated using experimental data from batch systems fed with FVW of varying particle sizes (ranging from 1.8 to 1000 μm). Response surface methodology establishes a statistical model for estimating methane production based on particle size and concentration. Numerical and statistical analyses were conducted using Matlab R2024a and Minitab 24 software. A model with an R2 of 0.89 was obtained, which determined an optimal concentration of 8.2 kg·m−3 and a particle size of 742.3 μm, yielding a methane production of 303.3 m3·kg−1 VS, similar to the experimentally obtained range of 300.95 to 316.7 m3·kg−1 VS.

ПОЛУЧЕНИЕ КОРМОВОГО СМУЗИ ИЗ ОТХОДОВ ОВОЩЕЙ И ФРУКТОВ

Background. Currently, about 50-60% of vegetable and fruit waste is generated at fruit and vegetable storage facilities, retail stores, distribution centers of retail chains from the total volume of discarded products. One of the solutions to reduce waste is to propose a way of their processing: fodder smoothie production. Purpose. To select technological processing modes for obtaining a sample mixture of fodder smoothie from vegetables and fruits. Materials and methods. For obtaining a model mixture of fodder smoothie 30% of tomatoes, 10% of potatoes and 20% each of carrots, cabbage, apples were used. To work out the technological parameters of obtaining the sample mixture, the following were recorded: grinding time, grinding speed, appearance of the obtained model mixture, as well as pH and mass fraction of soluble solids were determined.Results. The pH of the sample mixture of fodder smoothie was 6.4, the value of the mass fraction of soluble solids ranged from 14.2% to 16% depending on the parameters of producing the mixture. The sample mixture was mushy of brown color. Conclusion. The technological parameters were selected for the investigated sample mixture. The pH of the crushed product does not change with the change of technological parameters. Mass fraction of soluble solids increases with increasing the amount of product grinding time and the grinding speed.

Short Communication: Taxonomic Profiling of Bacteria in The Bio-Enzyme That Produced From Orange Fruit Waste

AbstractFruit wastes are reported as among the main contributors to food waste production. They have an impact on the environment so they need to be treated. To reduce the negative effects, fruit wastes must be treated before they are released into the environment. Meanwhile, the fruit wastes have been reported about their functional properties and nutritional as bio-enzyme. This preliminary study aimed to provide information about metagenome bacteria of bio-enzymes from orange fruit wastes treated with different concentrations of probiotics in anaerobic fermentation. Bio-enzyme was a fermented solution made from a mixture of 15 g brown sugar: 5 kg orange fruit waste: and 12 L water. The fermentation process was carried out for 1 month and then the bioenzyme sample was taken 1 L for metagenome analysis. The metagenomic analysis was carried out using the next-generation sequencing (NGS) method for the 16S rRNA gene of bacteria by amplicon full-length sequencing with nanopore sequencing. In this research, phylum Pseudomonadota (Proteobacteria), class Betaproteobacteria, order Burkholderiales, and family Comamonadaceae were the predominant bacterial group of the bio-enzyme that produced from orange fruit waste. Identification of bacterial species in the next research becomes important to understand the role of bacterial species in the biochemical metabolism of bio-enzyme products from orange fruit waste.AbstrakLimbah buah-buahan dilaporkan sebagai salah satu penyumbang utama produksi limbah makanan. Limbah buah mempunyai dampak terhadap lingkungan sehingga perlu ditangani. Limbah buah harus diolah terlebih dahulu untuk mengurangi dampak negatifnya sebelum dibuang ke lingkungan. Sementara itu, limbah buah telah dilaporkan mengenai sifat fungsional dan nutrisinya sebagai bio-enzim. Penelitian awal ini bertujuan untuk memberikan informasi mengenai diversitas metagenom bakteri yang terdapat di produk bioenzim yang diproduksi dari limbah buah jeruk yang diberi perlakuan konsentrasi probiotik berbeda dalam fermentasi anaerobik. Bio-enzim merupakan larutan fermentasi yang terbuat dari campuran 15 g gula merah : 5 kg limbah buah jeruk : 12 L air. Proses fermentasi dilakukan selama 1 bulan dan kemudian sampel bioenzim diambil sebanyak 1 L untuk dianalisis metagenom. Analisis metagenomik bakteri dilakukan dengan menggunakan metode next-generation sequencing (NGS) untuk analisis 16S rRNA gene melalui sekuensing amplikon dengan nanopore sequencing. Pada penelitian ini, keberadaan bakteri pada bio-enzim yang diproduksi dari limbah buah jeruk. Filum Pseudomonadota (Proteobacteria), kelas Betaproteobacteria, ordo Burkholderiales, dan famili Comamonadaceae merupakan kelompok bakteri yang dominan di bio-enzim yang diproduksi dari limbah buah jeruk. Identifikasi spesies bakteri pada penelitian selanjutnya menjadi penting untuk mengetahui peranan spesies-spesies bakteri di metabolisme biokimiawi dari produk bio-enzim dari limbah buah jeruk.

Preliminary biogas production assessment on insect frass and leachates of the organic wastes fed to larvae: A Johannesburg‐based factory case study

AbstractThe biomethane potential of insect frass and leachates from waste (larvae feedstock) stockpile was evaluated. Frass was derived from a black soldier fly larvae (BSFL) operation that uses a fruit and vegetable waste diet. The two waste streams were characterized and the results of their compositional, proximate, and ultimate analysis were used in estimating methane yields from these materials. The estimated biomethane potential (BMP) of the frass ranged 149–275 L/kgVS depending on the calculation method used. This result compares well with that of empirical studies for cow manure whose BMP ranges 110–275 L/kgVS. Cow manure is the most popular biogas feedstock globally. Leachates reported an estimated BMP of 150 L/kgCOD. We conclude that it may be technically feasible to produce biogas from BSFL frass and from fruit and vegetable waste leachates. However, standard BMP experiments and semicontinuous pilot studies are still necessary before commercialization of these findings. These extra tests are executed to validate these findings and ascertain the life cycle attribute and long‐term process stability and hence determine the economics of using these potential substrates for biogas production as a strategy to implementing circularity in the fruit and vegetable industry.

Evaluation of a pilot-scale anaerobic biofilm reactor for the treatment of slaughterhouse effluents in rural areas

ABSTRACT Rural slaughterhouses often lack strict effluent treatment rules, resulting in inadequate treatment prior to release into water bodies. Slaughterhouse wastewater (SWW) is a high-strength effluent, posing risks of contamination to ecosystems and human health. To tackle the need for suitable technologies in these areas, this study evaluated a 215 L pilot-scale fixed-bed biofilm reactor (FBBR), known for its simplicity in implementation, operation, and maintenance. During the operation, an initial startup period, with the liquid fraction of fruit and vegetable waste (LF-FVW) was conducted, followed by a gradual transition to SWW, creating a co-digestion period during the substrate transition. Organic load variations (1.59, 3.35, and 4.00 kgCOD/m3·d), corresponding to flow rates of 50, 75, and 120 L/d, respectively, were implemented to address potential alterations in effluent concentration and composition in authentic rural settings. Removal rates of up to 83.69, 91.81, 87.00, and 92.00% were achieved for COD, sCOD, TS, and VS, respectively, with an average removal of 32.00% for TN, and biogas production exceeded 300 L/h at 4.00 kgCOD/m3·d. These results suggest that FBBR implementation in rural areas effectively confronts SWW treatment challenges by reducing contamination, generating biogas, and adapting to rural conditions.

Use of Mixed Fruit Waste Aimed at Producing Bioethanol and Extracting D-Limonene

Use of Mixed Fruit Waste Aimed at Producing Bioethanol and Extracting D-Limonene

The Valorization of Fruit and Vegetable Wastes Using an Anaerobic Fixed Biofilm Reactor: A Case of Discarded Tomatoes from a Traditional Market

Tomato waste, characterized by high organic matter and moisture content, offers a promising substrate for anaerobic digestion, though rapid acidification can inhibit methanogenic activity. This study investigated the performance of a 10.25 L anaerobic fixed biofilm reactor for biogas production using liquid tomato waste, processed through grinding and filtration, at high organic loading rates, without external pH control or co-digestion. Four scouring pads were vertically installed as a fixed bed within a fiberglass structure. Reactor performance and buffering capacity were assessed over three stages with progressively increasing organic loading rates (3.2, 4.35, and 6.26 gCOD/L·d). Methane yields of 0.419 LCH4/gCOD and 0.563 LCH4/g VS were achieved during the kinetic study following stabilization. Biogas production rates reached 1586 mL/h, 1804 mL/h, and 4117 mL/h across the three stages, with methane contents of 69%, 65%, and 72.3%, respectively. Partial alkalinity fluctuated, starting above 1500 mg CaCO3/L in Stage 1, dropping below 500 mg CaCO3/L in Stage 2, and surpassing 3000 mg CaCO3/L in Stage 3. Despite periods of forced acidification, the system demonstrated significant resilience and high buffering capacity, maintaining stability through hydraulic retention time adjustments without the need for external pH regulation. The key stability indicators identified include partial alkalinity, effluent chemical oxygen demand, pH, and one-day cumulative biogas. This study highlights the effectiveness of anaerobic fixed biofilm reactors in treating tomato waste and similar fruit and vegetable residues for sustainable biogas production.

Pelatihan Strategi Branding dan Marketing Mix Produk Ecoenzym di Desa Landbaw Kecamatan Gisting Kabupaten Tanggamus

Household waste has not been utilized properly and is generally considered as garbage. In fact, it can be utilized as an ecoenzyme organic fertilizer that is not only beneficial for the soil and plants themselves, but can also reduce household waste in the form of fruit and vegetable waste. In general, household waste in Landbaw Village, Gisting District, Tanggamus Regency has been utilized properly as organic fertilizer. However, it has not been optimal. This is due to the absence of branding and marketing itself in order to introduce and distribute it to the wider community. Therefore, community service activities were held in Landbaw Village, Gisting District, Tanggamus Regency through counseling on how to package a product to make it more attractive and make other people trust our products so as to increase income.

Use of Fruit Waste as Natural Dyes in pH-Sensitive Colorimetric Sensors for Tilapia Fillets Quality Decline

Graphical Abstract Highlight Research 1. The use of dragon fruit peel, mangosteen peel and onion peel waste as natural dyes in colorimetric sensors was analyzed in this study. 2. Natural dyes are added to bioplastics with kappa carrageenan polymer, a mixture of corn starch and kappa carrageenan, and corn starch to determine the characteristics of the best bioplastic to be used as a colorimetry sensor. 3. The quality of the fish fillet compared to the color changes in colorimetry sensor was analysed in this study. 4. Mixture of kappa carrageenan and corn starch as bioplastic with casting method was analyzed in this study. Abstract Tilapia fillet aimed to prolonging shelf life, may still experience quality deterioration posing food safety risks. Colorimetric pH indicators offer a simple and affordable solution to the food industry to evaluating fish spoilage. Natural dyes reduce potential health risks associated with synthetic dyes. Anthocyanin sources haven’t been explored like dragon fruit rind, mangosteen rind, and red onion skin serve as real time quality and safety assessment tools for consumers. This study explores the potential of anthocyanin extracted from dragon fruit rind, mangosteen rind, and red onion skin as colorimetric sensors for evaluating the quality and safety of tilapia fillets. The anthocyanin-based sensors developed using kappa carrageenan and corn starch polymers to achieve sustainability fisheries program and were characterized according to the Japanese Industrial Standard for bioplastics. The results show that anthocyanin from dragon fruit skin exhibited the best color change in response to pH changes in the tilapia fillet, indicating its potential as a reliable indicator of spoilage. This research highlights the feasibility of using natural dyes as colorimetric sensors, reducing the risk of health hazards associated with synthetic dyes. This study also shows that different polymers give different characteristic of bioplastic. Carrageenan bioplastic shows best thickness values of 0,118 mm; Carrageenan and corn starch bioplastics shows best tensile strength values of 20,62 MPa; Carrageenan bioplastic shows best elongation values of 254%; and All polymers shows the same biodegradation rate values of 14,29%. Further studies are needed to explore other natural dyes and optimize the polymers for optimal bioplastic characteristics.

Pretreatment of Camellia oleifera shell by ethanolamine-based solvents for selective delignification and enhanced enzymatic saccharification

Fruit shell biomass is an agricultural waste that could be valorised into various value-added chemicals. However, the intertwined structure between lignin and holocellulose limits free sugar generation for further fermentation, requiring efficient pretreatment for selective delignification. We pretreated Camellia oleifera shell by ethanolamine-based solvents (binary deep eutectic solvents and single ethanolamine or its derivatives), and compared pretreatment effectiveness of these solvents. Single ethanolamine was the best among ethanolamine-based solvents, indicating the unnecessity of deep eutectic solvent synthesis. Furthermore, structural characterization confirms that ethanolamine could effectively deconstruct Camellia oleifera shell with the highest delignification (76.46%) and sugar yields (83.73% for glucose and 66.87% for xylose) at 130 °C for 3 h. Additionally, correlation analysis suggests that alkalinity and proton dissociation capacity together determined the delignification capacity of ethanolamine. Our findings serve a potential solution to valorise the waste fruit shell lignocellulose resources.

Characterization of fresh biowastes for biogas production and environmental health in Niger Delta, Nigeria

BackgroundEnvironmental pollution is a public health problem in Niger Delta, Nigeria. Therefore, the aims of the present study were to: identify the major fresh biowastes in Bayelsa State, Niger Delta; quantify the biogas yields from mono-digestion and co-digestion of identified biowastes; determine the first day of biogas production during hydraulic retention time; assess pH variations during anaerobic digestion; and evaluate biogas flame colours.MethodsFifteen communities in Bayelsa State were randomly selected, and on-the-spot assessment and quantification of the biowastes found in each community were carried out daily per week. Mono-digestion of 20 kg of each biowaste and co-digestion of 10 kg of animal waste with 10 kg of plant waste were carried out respectively under anaerobic conditions. Cumulative biogas yield and pH were measured using a pH meter and weighing scale respectively. Biogas flame colours during combustion were visually assessed.ResultsExactly 120.61 metric ton of fresh biowastes was found to be generated per week in Bayelsa State, Niger Delta. Industrial biowastes were the highest [47.6 tonnes, (39.46%)], followed by abattoir biowastes [33 tonnes (27%)], market and roadside sellers biowastes [25.5 tonnes (21.14%)], and farm biowastes [14.51 (12.03%)]. Biogas yields (kg) were: 0, 0, 0, 0, 0, 17, 14, 2, 18, 16, 17, 15 and 1 kg for palm oil mill effluent (POME), orange fruit waste (OFW), pineapple peels (PP), plantain peels, cassava mill effluent (CME), rumen digesta (RD), cow dung, sewage, PP-RD, plantain peels-cow dung, POME-rumen digesta, OFW-cow dung, and CME-sewage respectively. The first day of biogas production for RD, cow dung, sewage, PP-RD, plantain peels-cow dung, POME-RD, OFW-cow dung, and CME-sewage was on the 6th, 32nd. 56th, 1st, 26th, 18th, 25th, and 60th day of hydraulic retention time respectively. A dominant blue flame colour mixed reddish yellow or orange flames were found during biogas combustion. A slight increase in pH was found in all the biodigester media.ConclusionsIn the present study, a variety of biowastes yielding various quantities and qualities of biogas were identified in Bayelsa State, Niger Delta. The study’s findings have provided evidence-based data that might be explored as a road map and catalyst for policy creation against inadequate biowaste management and as sustainable alternatives to the expensive liquefied petroleum gas. The potential of current research study to be scaled up for commercial use is implicated in the present study.

Sustainable utilization of fruit and vegetable waste for the extraction of phenolics, antioxidants, and other valuables

Sustainable utilization of fruit and vegetable waste for the extraction of phenolics, antioxidants, and other valuables

Hepatoprotective Effect of Ethanolic Pomegranate Peel Extract Against Levofloxacin via Suppression of Oxidative Stress, Proinflammation, and Apoptosis in Male Rats.

One of the fluoroquinolone antibiotics, levofloxacin (LEV), is used to treat a variety of illnesses leading to oxidative stress and cellular damage. Peel from Punica granatum is a waste product abundant in phytochemicals with various biological activities. This study aimed to evaluate P. granatum peel extract's (PGPE) potential to mitigate oxidative stress, inflammation, apoptosis, and liver damage caused by LEV. There were four groups of rats: control, PGPE, LEV, and PGPE + LEV, respectively, and they were orally administered their daily treatments for 2 weeks. Results revealed that PGPE has a large number of phytochemical components with high antioxidant activity. PGPE intake alone enhanced the antioxidant status and decreased oxidative stress. On the other hand, pretreatment of the LEV group with PGPE restored oxidative stress, antioxidant enzymes, glutathione content, liver function biomarkers, and hematological parameters. Also, normalization of gene expressions (cyclooxygenase-2, transforming growth factor-beta1, caspase-3, heme oxygenase-1, B cell lymphoma-2, interleukin [IL]-10, and IL-1) and improvement in liver architecture, and immunohistochemical alpha-smooth muscle actin, were seen in comparison to the LEV group. Conclusively, PGPE exhibits strong anti-inflammatory, antiapoptotic, and antioxidant properties that shield rat liver from the damaging effects of LEV and offer a fresh viewpoint on the application of fruit waste products.

Using Household Fruit and Vegetable Waste in Recipes to Reduce Kitchen Food Waste and their Nutritional and Functional Values

The formation of food waste brings numerous issues such as inefficient use of nutrients, social injustice, and economic and ecological losses. The aim of this study is to reduce fruit and vegetable waste, which are among the most common kitchen wastes, with new recipes, and to explore the advantages they provide to human health. The study developed 20 different recipes using parts typically considered waste in the kitchen, like stems, stalks, and peels from 11 fruits and 9 vegetables, and calculated their nutritional values using the BEBIS program (Nutrition Information System). Additionally, the functional properties of the fruit and vegetable waste used in the study were examined in light of scientific literature. Results showed that parts of fruits and vegetables considered as waste might have valuable nutritional properties, such as being a good source of dietary fiber, antioxidants, vitamins, and minerals. Among the recipes created with fruit waste, the chocolate balls with pomegranate peel powder had the highest energy value (4255.3 kcal), the sweet chicken with orange flavor had the highest protein content (122.1 g), and the watermelon dessert had the highest fiber content (25.3 g). Among the recipes made with vegetable waste, the puff pastry with leek leaves had the highest energy value (2740 kcal), and the savory cake with cauliflower and potatoes contained the most protein (222.2 g) and fiber (184.1 g). Using parts like stems, peels, and leaves considered food waste in recipes also imparts functional properties to the recipes. The food waste used in this study possesses properties in the literature such as anti-cancer, antimicrobial, antiviral, anti-inflammatory, prevention of neurological disorders, and anti-obesity, anti-diabetes, and anti-aging effects.

Emerging ways to extract lycopene from waste of tomato and other fruits, a comprehensive review

Emerging ways to extract lycopene from waste of tomato and other fruits, a comprehensive review

Penanganan Pencemaran Tanah Pada Sampah Organik dengan Menggunakan Media Maggot

Based on observed research data, the volume of organic waste is increasing along with population growth, the number of consumers using food, and even the number of people interested in living necessities is increasing. With the increasing volume, it can be handled using the maggot media program to handle soil pollution by organic waste. From the data obtained, it was found that decomposition was faster in soft fruit waste and soft vegetable waste, then decomposition took longer in hard vegetable waste. This is because maggots, or fly larvae, more often choose to consume soft vegetable waste than hard ones.

Global Warming Mitigation Innovation Through Household Waste Management Becomes Eco-Enzyme: A Review

The dumping of fruit and vegetable waste into landfills contributes to an increase in methane gas that contributes to global warming. Waste materials in the form of fruits and vegetables can be used and processed into eco-enzymes. Fermented waste enzymes have a variety of benefits and add value to materials that have been tended to be wasted. This environmentally friendly enzyme has been utilized ranging from disinfectants, floor cleaners, dishwashers to liquid organic fertilizers. The production of eco-enzymes in addition to being able to reduce the volume of household waste, also contributes to the reduction of methane gas. During the fermentation process produces O3 (ozone) which is beneficial in maintaining ozone content in the atmosphere. This means that eco-enzyme production becomes an innovation in mitigating global warming from the household level. The manufacture of eco-enzymes is an effort to provide economic value to waste that has a positive impact on the environment and is a step to maximize the utilization of natural resources better known as circular economies. The advantages of eco-enzyme production in the short term are the ability to maximize raw materials and provide economic value and reduce the cost of handling household waste.

Tomato seed fractions enriched muffins: functional, textural, and qualitative characterisation

SummaryThe current study was undertaken to investigate the use of tomato seed oil (TSO) and defatted tomato seed meal (DTSM) individually and in combination for muffin preparation in an effort to utilise the fruit waste generated during food processing and harness its nutritional potential. The incorporation of DTSM and TSO at 0–25% led to a significant increase in the baking yield and weight of muffins, with a more pronounced effect in the case of DTSM‐incorporated muffins. Based on organoleptic evaluation, muffins with incorporation of DTSM and TSO at 20% and 15%, respectively, were most acceptable. Therefore, muffins (DTSM 20 + TSO 15) were prepared with DTSM and TSO replacement at 20% and 15%, respectively. The combined effect of DTSM and TSO resulted in a dietary fibre increase of 61.57% as compared to the control (wheat flour and margarine‐based muffins). As per texture profile analysis, the addition of DTSM led to a significant decrease in hardness, whereas a rise was noted for TSO‐incorporated muffins. Storage studies of muffins for 7 days in low‐density polyethylene pouches indicated no significant degradation in quality parameters. The results obtained in the study confirmed the efficient utilisation of tomato seed fractions for the preparation of nutritionally enriched muffins.

Production of Single Cell Protein (SCP) from the Peel Waste of Pea, Potato, and Banana by Aspergillus Flavus NRRL 21882 as an Efficient Organic Poultry Supplement.

Food protein deficit has become a major issue worldwide, particularly in underdeveloped countries. Scientists are searching for a variety of less expensive solutions to this issue. One of these less expensive methods is to create single cell protein as a substrate from leftover fruit and vegetable waste, which is typically thrown away. In this regard, the fungal strain Aspergillus flavus (NRRL 21882) was used for the synthesis of SCP (single cell protein) from the waste of banana, potato, and pea. In this manner, 30 samples were collected from the whole substrate with a share of 10 samples each from banana, potato, and pea peels, which were in turn dried and powdered finely. The fermentation process was done by the process of solid state fermentation. Aspergillus flavus (NRRL 21882) generated the highest percentage, i.e. 60.67%, of crude protein from the pea peels. The composition of amino acids in crude proteins was also investigated. The findings demonstrated that the highest percentage of aspartic acid (13.34 ± 0.80%) and glutamic acid (14.92 ± 0.69%) was found in A. flavus single cell protein produced from pea peels. Soybean was supplemented with single cell protein in the boilers' diet. Compared to all treated groups, there was a substantial (p ≤ 0.05) increase in the level of antibody titer against the Newcastle disease vaccine. The supplementation of single cell protein with soybean meal had no effect on the levels of liver enzymes. The liver enzymes found in all four groups (A, B, C, and D) were within normal limits. None of the examined groups experienced any change in the feed conversion ratio, with all groups exhibiting an average FCR of 1.6. The current study concludes that broiler health and immunity is increased by supplementing poultry feed with single cell protein.