Loss Of Vitality Research Articles

Microbial Photoinactivation by Visible Light Results in Limited Loss of Membrane Integrity.

Interest in visible light irradiation as a microbial inactivation method has widely increased due to multiple possible applications. Resistance development is considered unlikely, because of the multi-target mechanism, based on the induction of reactive oxygen species by wavelength specific photosensitizers. However, the affected targets are still not completely identified. We investigated membrane integrity with the fluorescence staining kit LIVE/DEAD® BacLight™ on a Gram positive and a Gram negative bacterial species, irradiating Staphylococcus carnosus and Pseudomonas fluorescens with 405 nm and 450 nm. To exclude the generation of viable but nonculturable (VBNC) bacterial cells, we applied an ATP test, measuring the loss of vitality. Pronounced uptake of propidium iodide was only observed in Pseudomonas fluorescens at 405 nm. Transmission electron micrographs revealed no obvious differences between irradiated samples and controls, especially no indication of an increased bacterial cell lysis could be observed. Based on our results and previous literature, we suggest that visible light photoinactivation does not lead to rapid bacterial cell lysis or disruption. However, functional loss of membrane integrity due to depolarization or inactivation of membrane proteins may occur. Decomposition of the bacterial envelope following cell death might be responsible for observations of intracellular component leakage.

In Vivo Transcutaneous Monitoring of Hemoglobin Derivatives Using a Red-Green-Blue Camera-Based Spectral Imaging Technique.

Cyanosis is a pathological condition that is characterized by a bluish discoloration of the skin or mucous membranes. It may result from a number of medical conditions, including disorders of the respiratory system and central nervous system, cardiovascular diseases, peripheral vascular diseases, deep vein thrombosis, and regional ischemia. Cyanosis can also be elicited from methemoglobin. Therefore, a simple, rapid, and simultaneous monitoring of changes in oxygenated hemoglobin and deoxygenated hemoglobin is useful for protective strategies against organ ischemic injury. We previously developed a red-green-blue camera-based spectral imaging method for the measurements of melanin concentration, oxygenated hemoglobin concentration (CHbO), deoxygenated hemoglobin concentration (CHbR), total hemoglobin concentration (CHbT) and tissue oxygen saturation (StO2) in skin tissues. We leveraged this approach in this study and extended it to the simultaneous quantifications of methemoglobin concentration (CmetHb), CHbO, CHbR, and StO2. The aim of the study was to confirm the feasibility of the method to monitor CmetHb, CHbO, CHbR, CHbT, and StO2. We performed in vivo experiments using rat dorsal skin during methemoglobinemia induced by the administration of sodium nitrite (NaNO2) and changing the fraction of inspired oxygen (FiO2), including normoxia, hypoxia, and anoxia. Spectral diffuse reflectance images were estimated from an RGB image by the Wiener estimation method. Multiple regression analysis based on Monte Carlo simulations of light transport was used to estimate CHbO, CHbR, CmetHb, CHbT, and StO2. CmetHb rapidly increased with a half-maximum time of less than 30 min and reached maximal values nearly 60 min after the administration of NaNO2, whereas StO2 dramatically dropped after the administration of NaNO2, indicating the temporary production of methemoglobin and severe hypoxemia during methemoglobinemia. Time courses of CHbT and StO2, while changing the FiO2, coincided with well-known physiological responses to hyperoxia, normoxia, and hypoxia. The results indicated the potential of this method to evaluate changes in skin hemodynamics due to loss of tissue viability and vitality.

The Symptom Structure of Seasonal Affective Disorder: Integrating Results from Factor and Network Analyses in the Context of the Dual Vulnerability Model

Research on seasonal affective disorder (SAD) has produced several etiological models of SAD symptomatology, including a common cause model that conceptualizes symptoms as the result of a single underlying disease process and the Dual Vulnerability Model (Young et al., Journal of Affective Disorders, 22, 191–197 1991) which posits that cognitive/affective symptoms of depression are responses to vegetative symptoms (fatigue, hypersomnia, increased appetite) in individuals with a cognitive vulnerability to these seasonal changes. This study used exploratory factor analysis and network analyses to examine 22 symptoms of winter SAD in 177 adults at the start of a randomized SAD treatment trial (Rohan et al., American Journal of Psychiatry, 172, 862–9 2015). The factor analysis supported a novel four-factor model that included Negative Cognition, Loss of Vitality, Increased Appetite/Weight and Dysregulation across symptom domains. The results of the network model of interacting symptoms paralleled those of the factor analysis in producing four clusters of inter-correlated symptoms. A directed acyclic graph was constructed to model possible causal relations between symptom factors/clusters. Results suggest that vegetative symptoms (Loss of Vitality and Appetite/Weight) contribute to Dysregulation and that Loss of Vitality and Dysregulation contribute to Negative Cognition, supporting the overall structure of the Dual Vulnerability Model but with greater symptom differentiation. Findings have implications for how SAD should be researched (e.g., clusters vs. diagnosis or individual symptoms), opportunities for intervention, and the expected course of onset and remission.

Loneliness in older people: Spiritual practices as an alternative pathway to action, a treatise from India

Loneliness is an abstract construct defined in multiple ways. It is a feeling of disconnectedness, emotional isolation, and subjective feeling of lacking social relationships. Research shows loneliness to be common in older people, which, in turn, is a potent risk factor for various physical and psychosocial health conditions. Chronic loneliness is a predisposing factor for suicide, worsens cognitive outcomes, and impairs quality of living. Conventionally, aging is associated with “loss of vitality” and the “desperate need to retain older abilities.” In contrast to these concepts, eastern views center around acceptance, “letting go” and aging with a greater sense of purpose. Traversing loneliness with self-esteem is viewed over a consistent resistance against loneliness. Self-acceptance, wisdom, and understanding the processes of emotional aging can foster hope and resilience which help navigate the inevitable loneliness that may arise in old age due to a multitude of factors. Spirituality has various intersections with mental well-being, however, it is often considered to be an esoteric concept. In this paper, we provide an Indian understanding of accepting and dealing with loneliness through the daily implementation of spiritual practices in life as well as mental health interventions. The four ashramas of Hinduism are discussed with special relevance to Vanaprastha Ashrama (forest-dweller), which signifies renunciation and acceptance associated with aging. The actionable areas with respect to spirituality and its philosophical underpinnings in mitigating loneliness in older people are also highlighted.

Michel Houellebecq’in Teslimiyet Romanında Asabiyet Bozumu ve Kültürel İntihar

This article identifies the correspondences that Ibn Khaldun’s concepts of asabiya, mulk and dynasty have in Michel Houellebecq’s 2015 novel Submission. It analyses the work of a contemporary author through the method sketched out by a Muslim scholar of the 14th century, thereby provincializing a European text, and showing the continuities of cultural thought in the Mediterranean. While putting the role of asabiya at the centre, the article deploys Dipesh Chakrabarty’s understanding of provincialization, and Fernand Braudel’s concept of encounters in the Mediterranean. In Submission, Houellebecq describes a France in which a Muslim candidate has become the president. As the perceived source of Western European culture, Mediterranean has become uncanny, and Houellebecq’s novels reflect this uneasy relationship between the continent and the basin. Houellebecq’s narrator reflects how the decline of France and its apparent cultural suicide is due to lack of solidarity between the classes, how White French people are very quick to adopt the ways of the Maghrebi immigrants, and how, ultimately, the Maghrebi immigrants who are now in power will suffer the same loss of vitality as their White French predecessors. These observations comply with the cyclical social behaviour that Ibn Khaldun has mapped out in the Muqaddimah.

SHED, PRF, and Chitosan as Three-Dimensional of Tissue Engineering for Dental Pulp Regeneration

The gold standard for pulpitis irreversible treatment is root canal treatment. However, it caused the loss of tooth vitality. To restore tooth vitality, materials that have regenerative ability in the pulp is needed. The exfoliated deciduous teeth stem cells (SHED) not only expressed specific markers for mesenchymal stem cells (MSC) but also induced the odontoblastic differentiation, and stimulating the formation of endothelium and fibroblast. The combination SHED with platelet rich fibrin (PRF) and chitosan were able to facilitate and increase the migration, proliferation and odontoblastic differentiation of dental pulp cells. Based on that fact, the combination of SHED, PRF and chitosan as three-dimensional tissue engineering is promising as new modality to pulp regeneration in the clinical setting. The purpose of this review is describing the potential combination of SHED, PRF, and chitosan scaffold as three-dimensional tissue engineering for pulp regeneration.

Efficacy of direct pulp capping for management of cariously exposed pulps in permanent teeth: a systematic review and meta-analysis.

The outcome of vital pulp treatment after carious pulp exposure is multifactorial and related to the procedure, biomaterial and pre-operative pulpal diagnosis. To conduct a systematic review and meta-analysis determining the outcome of direct pulp capping (DPC) in mature permanent teeth with a cariously exposed pulp and a clinical diagnosis of reversible pulpitis, and ascertain whether the capping material influences the outcome. Sources: MEDLINE Ovid-SP, Cochrane Central Register of Controlled Trials (CENTRAL), International Clinical Trials Registry Platform (ICTRP), ClinicalTrials.gov, Embase and Web of Science until April 2020. Inclusion: Prospective, retrospective cohort studies and randomized trials investigating DPC outcome or comparing different capping materials after carious pulp exposure. Exclusion: Primary teeth, mechanical, traumatic or not specified pulp exposure, teeth with irreversible pulpitis or no pulpal diagnosis. Risk of bias assessed using Cochrane and modified Downs and Black quality assessment checklist. Meta-analysis on combined clinical/radiographic outcome was performed using a random effect model. Success was defined as absence of signs and symptoms of irreversible pulpitis, apical periodontitis or loss of pulp vitality. Quality assessment highlighted four non-randomized studies to be of fair and five of poor quality. Four randomized trials had a high risk of bias. The pooled success rate differed based on material and follow-up. Calcium hydroxide success rate was 74% at 6-months, 65% at 1-year, 59% at 2-3years and 56% at 4-5years. Mineral trioxide aggregate (MTA) success was 91%, 86%, 84% and 81% at the same time points. Biodentine success was 96% at 6-months, 86% at 1year and 86% at 2-3years. The meta-analysis revealed MTA had better success than calcium hydroxide at 1-year (OR 2.66, 95% CI; 1.46- 4.84, P=0.001) and 2- to 3-year follow-up (OR 2.21, 95% CI; 1.42-3.44, P=0.0004). There was no difference between MTA and Biodentine. These results were based on poor methodological quality studies. The effect size for of MTA vs Ca(OH)2, although modest, was consistent with narrow CI. Low-quality evidence suggests a high success rate for direct pulp capping in teeth with cariously exposed pulps with better long-term outcomes for MTA and Biodentine compared with calcium hydroxide.

The use of MTA in the modern management of teeth affected by dens invaginatus

Dens invaginatus is a dental anomaly that can result in loss of tooth vitality and the need for root canal treatment. The pulpal morphology of these teeth can be complex which makes successful root canal treatment difficult to achieve. To maintain vitality attempts have been made to prophylactically treat these teeth by sealing the invagination with a variety of materials. This paper describes the use of mineral trioxide aggregate (MTA) in both the prophylactic treatment of teeth with minor invaginations and the incorporation of MTA in the final obturation of non-vital teeth with invaginations with grossly atypical morphology.

Tissue Viability of Free Flaps after Extracorporeal Perfusion Using a Modified Hydroxyethyl Starch Solution.

Background: In free flap surgery, tissue is stored under hypothermic ischemia. Extracorporeal perfusion (EP) has the potential to extend storage time and the tissue’s perspective of survival. In the present study, the aim is to improve a recently established, simplified extracorporeal perfusion system. Methods: Porcine musculus rectus abdominis were stored under different conditions. One group was perfused continuously with a simplified one-way perfusion system for six hours, while the other received only a single flush but no further treatment. A modified hydroxyethyl starch solution was used as a perfusion and flushing solution. Vitality, functionality, and metabolic activity of both groups were analyzed. Results: Perfused muscles, in contrast to the ischemically stored ones, showed no loss of vitality and significantly less functionality loss, confirming the superiority of storage under continuous perfusion over ischemic storage. Furthermore, in comparison to a previous study, the results were improved even further by using a modified hydroxyethyl starch solution. Conclusion: The use of EP has major benefits compared to the clinical standard static storage at room temperature. Continuous perfusion not only maintains the oxygen and nutrient supply but also removes toxic metabolites formed due to inadequate storage conditions.

The efficacy of the anticancer 3-bromopyruvate is potentiated by antimycin and menadione by unbalancing mitochondrial ROS production and disposal in U118 glioblastoma cells

Metabolic reprogramming of tumour cells sustains cancer progression. Similar to other cancer cells, glioblastoma cells exhibit an increased glycolytic flow, which encourages the use of antiglycolytics as an effective complementary therapy. We used the antiglycolytic 3-bromopyruvate (3BP) as a metabolic modifier to treat U118 glioblastoma cells and investigated the toxic effects and the conditions to increase drug effectiveness at the lowest concentration. Cellular vitality was not affected by 3BP concentrations lower than 40 μM, although p-Akt dephosphorylation, p53 degradation, and ATP reduction occurred already at 30 μM 3BP. ROS generated in mitochondria were enhanced at 30 μM 3BP, possibly by unbalancing their generation and their disposal because of glutathione peroxidase inhibition. ROS triggered JNK and ERK phosphorylation, and cyt c release outside mitochondria, not accompanied by caspases-9 and -3 activation, probably due to 3BP-dependent alkylation of cysteine residues at caspase-9 catalytic site. To explore the possibility of sensitizing cells to 3BP treatment, we exploited 3BP effects on mitochondria by using 30 μM 3BP in association with antimycin A or menadione concentrations that in themselves exhibit poor toxicity. 3BP effect on cyt c release and cell vitality loss was potentiated due the greater oxidative stress induced by antimycin or menadione association with 3BP, supporting a preeminent role of mitochondrial ROS in 3BP toxicity. Indeed, the scavenger of mitochondrial superoxide MitoTEMPO counteracted 3BP-induced cyt c release and weakened the potentiating effect of 3BP/antimycin association. In conclusion, the biochemical mechanisms leading U118 glioblastoma cells to viability loss following 3BP treatment rely on mitochondrial ROS-dependent pathways. Their potentiation at low 3BP concentrations is consistent with the goal to minimize the toxic effect of the drug towards non-cancer cells.

Treatment outcome of full pulpotomy using Biodentine in cariously exposed vital permanent teeth: A case report

Preserving the vitality of pulp can never be overemphasized. Conventionally, pulp exposures were root canal treated but it results in loss of vitality of pulp. Vital pulp therapy maintains tooth vitality, functionality and to render tooth asymptomatic. Pulpotomy using Calcium silicate based cements could be a good alternative for RCT for management of symptomatic mature permanent teeth with carious pulp exposure, however large scale clinical trials are highly encouraged to confirm this hypothesis.

Loss of nonvital abutment teeth for removable partial dentures: a systematic review and meta-analysis.

To analyze the loss of nonvital abutment teeth compared to vital abutment teeth supporting removable partial dentures (RPDs). An electronic search was conducted in the Ovid MEDLINE, Embase, and Web of Science databases, and supplemented by a manual search. The search was done to identify clinical studies reporting the loss of nonvital abutment teeth compared to vital abutment teeth for RPDs. The data were extracted from each selected article and meta-analysis was performed using a random effects model to estimate adjusted proportion of abutment loss with RPDs along with 95% confidence interval (CI). Risk of bias assessment was conducted using Cochrane Risk of Bias tools and Newcastle-Ottawa Scale. The statistical significance was set as P value < .05. A total of 3,898 records were identified from electronic databases and 8 studies were included for quantitative synthesis of 834 subjects with 1,036 RPDs. No additional records were identified through manual search. Among 1,152 nonvital abutment teeth, 123 teeth were lost. The estimate of nonvital abutment loss was 13% (95% CI 9-18%]. The data were statistically significant (P < .0001), and were heterogenous between the studies (χ2 [df = 7] = 35.9, P < .0001; τ2 = 0.25, I2 = 76.46%). A total of 2,186 vital abutment teeth were compounded where 114 teeth were lost. The estimate of vital abutment loss was 4% (95% CI 2-7%). The data were statistically significant (P < .0001), and were heterogenous between the studies (χ2 [df = 7] = 23.7, P = .01; τ2 = 0.56, I2 = 86.31%). The risk of abutment loss was approximately three times greater with the loss of vitality (odds ratio = 3.04, 95% CI 1.53-6.05; P = .001). In addition, abutment loss was significantly greater with increasing follow-up time (P = .01). None of the included studies were considered to be at high risk of bias. Within the limitations of this systematic review and meta-analysis, the loss of nonvital abutment teeth was significantly greater than that of vital abutment teeth for RPDs. Further research is needed to identify critical factors associated with the loss of nonvital abutment teeth.

Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen.

Simple SummaryA significant effort has been devoted to the enhancement of boar sperm preservation media and techniques, which have become a critical pillar of modern swine production. Despite the availability of numerous semen extenders, a higher antioxidant protection of male gametes is highly required, which may be achieved by the supplementation of natural biomolecules such as quercetin and naringenin. In this regard, we have performed a number of experiments in order to define the optimal concentration range of both biomolecules that could ensure a higher structural integrity, functional activity, and antioxidant profile of boar spermatozoa subjected to short-term storage. The beneficial outcomes achieved in this study shall be tested in vivo at our collaborating pig farm, with their potential contribution to the optimization of the use of stored boar semen in the porcine breeding industry.In this study, we evaluated the impact of 5–50 μM quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p < 0.001 and p < 0.0001; 10 μM), and which correlated with the most prominent maintenance of sperm motility (p < 0.01, 48 h; p < 0.05, 72 h). A significant membrane stabilization (p < 0.01, 24 h and 48 h; p < 0.0001, 72 h) and prevention of lipid peroxidation (p < 0.05, 24 h and 48 h; p < 0.01, 72 h) was primarily observed following administration of 10 and 25 μM NAR; respectively. Administration of 10 μM QUE led to a significant decrease of superoxide (p < 0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 μM NAR (p < 0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.

Influence of Lateral Sinus Floor Elevation Procedures on the Pulpal and Periapical Status of Adjacent Vital Teeth

The aim of the present study was to evaluate the pulpal and periapical status of vital teeth within the area of a lateral sinus floor elevation (LSFE). The files of patients treated by LSFE between 2009 and 2015 were retrospectively evaluated. The radiographic bone measurements and the periapical status of the teeth adjacent to the LSFE area were evaluated preoperatively and 6 months following the surgery by CBCT. The pulpal status of the adjacent teeth was clinically evaluated using thermal pulp vitality testing. Vital teeth within the sinus floor elevation area were the main inclusion criteria for this study. A total of 158 LSFE procedures were evaluated; 18 cases fulfilled the inclusion criteria, and 20 adjacent teeth were vital. There were no cases of periapical pathology or loss of pulp vitality. There was a statistically significant difference in the ridge height between the baseline and 6 months postoperatively and in the distance from the apex to the sinus floor between pre- and 6 months postoperatively (p &lt; 0.01).The pulpal and periapical status of vital teeth was not affected following LSFE.

Dens Evaginatus – ‘Addition Beats Subtraction’

This case describes the presentation of dens evaginatus on a maxillary central incisor in an 8-year-old Caucasian male, its subsequent monitoring and later management. Dens evaginatus is a dental anomaly most commonly noted as an accessory tubercle on the occlusal surface of premolars and the palatal aspect of incisors as a talon cusp. Complications can result in loss of pulp vitality, sometimes causing periapical periodontitis. Clinicians should be aware of the presentation and the advantages of different approaches to the management of dens evaginatus.CPD/Clinical Relevance: To raise awareness of dens evaginatus in dental professionals working in primary and secondary care.

Mycological effects as a sign of a phytopathological situation in green landscape

Visual signs of the negative influence of various factors in woody plants include the loss of decorativeness and vitality, discolouration of needles, uneven growth, etc. Conifers of the boreal zone, including Siberian fir (Abies sibirica Ledeb.), have the most pronounced decrease in the ability of plants of green plantations to resist extreme environmental conditions. In perennial plantations of Siberian fir, scientists identified a complex of fungi distinguished not only by low biodiversity but also by the abundance of phytopathogens and moulds from the genera Alternaria, Diplodia, Macrophoma, Pestalotia, Sclerophoma, Cucurbitaria. The article shows that under stressed conditions (imbalance in nutrients, etc.), the fir plants in the root zone have critical disturbances or mycological effects which characterizes the deterioration of their viability and loss of resistance to disease. This data are useful for phytosanitary monitoring of green plantations and planning environmental protection measures in urban areas.

Harvesting of Autogenous Bone Graft from the Ascending Mandibular Ramus Compared with the Chin Region: a Systematic Review and Meta-Analysis Focusing on Complications and Donor Site Morbidity.

ABSTRACTObjectivesThe aim of this systematic review was to test the hypothesis of no difference in complications and donor site morbidity following harvesting of autogenous bone graft from the ascending mandibular ramus compared with the chin region.Material and MethodsMEDLINE (PubMed), Embase and Cochrane Library search in combination with a hand-search of relevant journals was conducted including human studies published in English through June 26, 2020. Randomized and controlled trials were included. Outcome measures included pain, infection, mucosal dehiscence, altered sensation or vitality of adjacent tooth/teeth, neurosensory disturbances and patient-reported outcome measures. Risk of bias was assessed by Cochrane risk of bias tool and Newcastle-Ottawa Scale.ResultsTen controlled trials of high-quality fulfilled inclusion criteria. Risk of infection and mucosal dehiscence seems to be comparable with the two treatment modalities. However, harvesting from the chin seems to be associated with increased risk of pain, altered sensation or loss of tooth vitality, and neurosensory disturbances. Willingness to undergo the same treatment again was reported with both treatment modalities, but significant higher satisfaction, lower discomfort and acceptance of the surgical procedure was reported following harvesting from the ascending mandibular ramus.ConclusionsThe hypothesis was rejected due to higher prevalence and severity of complications and donor site morbidity following harvesting of autogenous bone graft from the chin region. Dissimilar evaluation methods and various methodological confounding factors posed serious restrictions for literature review in a quantitative systematic manner. Conclusions drawn from results of this systematic review should therefore be interpreted with caution.

Evaluation of the anti-aging and antioxidant action of Ananas sativa and Moringa oleifera in a fruit fly model organism.

aging is an inevitable biological complex process. It involves the gradual loss of cellular vitality due to accumulative damage to cellular macromolecules by reactive oxygen species (ROS). These ROS are highly implicated in health, disease, and lifespan. The biochemical pathways involved in the aging process are highly influenced by both exogenous (environmental factors) and endogenous stress factors. These cellular processes are the same in most organisms including the fruit fly, nematode, yeast, mammalian cell line, and rodents. These model organisms have been extensively used in the screening of potent antioxidant botanicals for anti-aging bioactivity. Moringa oleifera and Ananas sativa are great sources of health-promoting nutrients and antioxidants, however, their anti-aging impact is still an evolving area of research interest. Therefore, this review focused on their anti-aging mode of action and some other anti-aging nutriceuticals in different model organisms including the fruit fly. PRACTICAL APPLICATIONS: Staying forever young and healthy is everyone's right. Aside from genetic trait, healthy feeding is peculiar to the world's longest-living people. Ananas sativa (pineapple) and Moringa oleifera leaves are highly valued fruit and herb with nourishing, antioxidant, and medicinal properties. Their extract exhibit antioxidant, anticancer, anti-inflammatory, and anti-aging activities. The ancient Greeks, Egyptians, and Romans used Moringa seed oil for cosmetics and perfumes. Moringa tea leaves is consumed for its nutritive and medicinal value. Its antioxidant potency endorses its use for anti-aging and other health-promoting purposes. The bioactive compound in pineapple, bromelain, promotes wound healing and it is a component of postsurgical applications due to its anti-inflammatory property. Consumption of Ananas fruit provides the recommended daily allowance of vitamin C, a potent antioxidant. To identify new anti-aging bioactive compounds of therapeutic importance, and understanding the mode of action of these nutriceuticals will contribute to new anti-aging research prospects.

Temporary Anchorage Device as an Innovative Alternative for Unilateral Arch Mesialization in Medical Application

Like any other medical intervention, orthodontic treatment has, in addition to its benefits, also associated risks and complications that are sometimes caused by wrongful treatment objectives. Correction of previous orthodontic malpractice is always a challenging problem in orthodontics. The present study aimed to introduce an innovative approach for total arch mesialization. In this study, the treatment course of a 25-year-old woman dissatisfied with her previous improper orthodontic treatment plan and unnecessary extraction of the upper and lower right first premolar teeth. Maxillary and mandibular dental midline deviation was evident concurrent with asymmetric dental arches and excessive gingival display a smile. The proposed corrective treatment plan consisted of right side temporary anchorage devices assisted total arch mesialization in both maxilla and mandible, followed by orthognathic surgery at the end. The patient’s final occlusion and facial profile improved significantly after the second treatment. The result was determined to be stable over a 1-year period. No significant evidence of root resorption was detected. Overbite decreased from 95% to 25%. Yaw correction was completely accomplished, and the final lateral cephalometric analysis after orthognathic surgery showed skeletal cl I relationship, normal proclination of upper and lower incisor teeth, and no evidence of periodontal complication and loss of vitality in the mandibular and maxillary teeth. The unilateral mesialization approach used in the current study prevented invasive therapies such as dental implants employing a special approach, which is less aggressive and more durable for the patient and revealed successful results with minimum side effects.

Tatalaksana Perawatan Doskolorasi Intrinsik Dengan Perawatan Saluran Akar Dan Metode Walking Bleach

Background: Intrinsic discoloration of the tooth can be caused following trauma, loss of vitality, endodontic treatment, and restorative procedures apart from known local and systemic factors. Tooth bleaching, veneering or placement of a full coverage crown are treatment options for discolored tooth. Non-vital teeth that are extensively discolored are highly receptive for bleaching techniques. This case report discusses a patient having discoloration in the anterior region with caries in proximal distal. In clinical aspects considerations, it was essential for a minimally invasive and achieve esthetic treatment. Objective: This case report is written to bring forward the proper and successful management in esthetic treatment of anterior maxilla in discoloration with caries in proximal case. Case and management : A 20 years old female patient complained of discoloration in 11 and feels uncomfortable with her condition. On examination, 11 caries D6 ICDAS, Mount &amp; Hume site 2 size 3 and Class IV G.V Black. In first appointment, patient was informed about the treatment and signed informed consent. Endodontics treatment was performed in multiple visit. Root canal preparation was performed using TF Adaptive 25/.08, 35/.06, 50/.04. Obturation used warm vertical compaction. After finishing the endodontics treatments 2 mm wing barrier was built to prepare the walking bleach method. Walking bleach using hydrogen peroxide 35% was applicated in pulp chamber for 1 week. In next visit, composite restoration was done. Conclusion: Management in intrinsic discoloration can be done by performing endodontic treatment , walking bleach combined with composite restoration and they can give satisfactory result.