Mapping cardiac conduction velocity may provide assessment of myocardial substrate, fiber anisotropy and zones of myocardium that may be critical to arrhythmia maintenance. However, validating new methodology is limited by the lack of a true reference standard. Optical mapping provides true local assessment of myocardial depolarization and true wave front mapping. To validate Omnipolar mapping technology derived wave speed (OT-WS) and wave direction (OT-DI) from an optical mapping standard. Optical mapping data was acquired from whole heart rabbit Langendorff model. Waves were produced by electrically stimulating the heart at the edge of the mapping field. Conduction velocity (CV) measurements were performed on a 4 x 4 array of virtual electrodes equally spaced by 2.4 mm, located in the middle of the mapping field. Traditional CV measurement methods based on local activation time (LAT, Dubois, Bayly) produced a gold standard of wave speed (LAT-WS) and direction (LAT-DI). These parameters were compared against OT-WS and OT-DI respectively. Beat to beat variability of speed and direction from both methods were also compared. 3 rabbit hearts experiments and 6 pacing locations were studied. Average OT-WS was 45.2 ± 10.6 cm/s, compared to 48.6 ± 14.6 for LAT_WS. OT-WS variability was comparable to LAT-WS (3.8 cm/s vs. 4.0 cm/s, p=0.85) as well as OT-DI variability versus LAT-DI (5.8° vs. 6.6°, p=0.76). OT measurements of speed and direction are comparable to gold standard in an optical mapping model without the need of LAT annotation. Beat-to-beat stability of OT measurements suggests OT might be superior predictor than LAT-based methods.