Coconut Oil Research Articles

Novel foam‐draining and anti‐polymerization integrated agent for gas well application

AbstractIn this article, surfactants such as cetyl trimethyl ammonium chloride (CTAC) and coconut oil amide propyl betaine (CAB) were compounded (TCJ) and used as a double function with foam drainage and hydrate anti‐polymerization. According to the result it was found that 0.05% of CTAC and 0.7% of CAB exhibited both excellent foam property and hydrate anti‐polymerization property. For further clarifying the stability characteristic of foam, the foam half‐life, drainage process, microcosmic liquid film structure, and stability mechanism were investigated in this article. The high initial foam volume of 415 mL with relatively long half‐life time of 5.5 min under high‐speed agitation at 50°C after 25 min indicated a good temperature resistance. The excellent foam properties still remained even amount of methanol and NaCl was added, which indicated its well stability and salt resistance. Furthermore, it was found that TCJ exhibited high liquid carrying rate of 62.5% at 65°C and good compatibility with poly vinyl pyrrolidone (PVP), which played great potential to the application in oil field. By the results of DSC curves, it can be found that the phase transformation point of hydrate decreases after addition of TCJ, which indicates the inhibition to the formation of hydrate as thermodynamic inhibitors.

Bacterial Cellulose-Silk Hydrogel Biosynthesized by Using Coconut Skim Milk as Culture Medium for Biomedical Applications.

In this study, hydrogel films of biocomposite comprising bacterial cellulose (BC) and silk (S) were successfully fabricated through a simple, facile, and cost-effective method via biosynthesis by Acetobacter xylinum in a culture medium of coconut skim milk/mature coconut water supplemented with the powders of thin-shell silk cocoon (SC). Coconut skim milk/mature coconut water and SC are the main byproducts of coconut oil and silk textile industries, respectively. The S/BC films contain protein, carbohydrate, fat, and minerals and possess a number of properties beneficial to wound healing and tissue engineering, including nontoxicity, biocompatibility, appropriate mechanical properties, flexibility, and high water absorption capacity. It was demonstrated that silk could fill into a porous structure and cover fibers of the BC matrix with very good integration. In addition, components (fat, protein, etc.) in coconut skim milk could be well incorporated into the hydrogel, resulting in a more elastic structure and higher tensile strength of films. The tensile strength and the elongation at break of BC film from coconut skim milk (BCM) were 212.4 MPa and 2.54%, respectively, which were significantly higher than BC film from mature coconut water (BCW). A more elastic structure and relatively higher tensile strength of S/BCM compared with S/BCW were observed. The films of S/BCM and S/BCW showed very high water uptake ability in the range of 400-500%. The presence of silk in the films also significantly enhanced the adhesion, proliferation, and cell-to-cell interaction of Vero and HaCat cells. According to multiple improved properties, S/BC hydrogel films are high-potential candidates for application as biomaterials for wound dressing and tissue engineering.

Structure changes and carotenoids release of tomato during in vitro dynamic digestion: Effect of heating and oil addition

There is still a gap between the food processing and carotenoids release and structure degradation during digestion. This study investigated the effect of heating and coconut oil addition on the digestion behavior of tomatoes during in vitro dynamic digestion. Coconut oil and heating increased gastric retention, and the value of heat-treated tomatoes with coconut oil maintained at a highest level throughout the gastric digestion. The contents of lycopene and β-carotene increased after heating and coconut oil addition. After stimulated intestinal digestion, coconut oil and heating increased the particle size of tomato slurry. Besides, heat-treated tomatoes released more particles under the light microscopy and SEM observation, while the oil-treated tomatoes showed rougher cell surfaces. Heating and coconut oil also significantly increased the bioaccessibility of lycopene to 70 % ± 5 % and 81 % ± 4 %, respectively. These findings would provide theoretical guidance to develop tomato-derived foods with high bioaccessibility.

Comparison of Coconut and Sunflower Seed Oils in Improving the Skin Integrity and Weight Gain of Infants in the Neonatal ICU

ABSTRACT OBJECTIVE To determine the effects of sunflower seed oil and coconut oil on the skin integrity and weight gain of preterm infants in the neonatal ICU. METHODS In this randomized controlled trial, 66 preterm neonates (34–37 weeks’ gestation) in the neonatal ICU of a training and research hospital were equally divided into three groups: sunflower seed oil, coconut oil, and control. The weights of neonates in all three groups were measured at admission to the neonatal ICU, at discharge, and at 1 month postdischarge. Their skin conditions were evaluated using the Neonatal Skin Condition Score and the Revised Northampton Neonatal Skin Assessment Tool at the same three time points. RESULTS At 1 month postdischarge, neonates in the sunflower seed oil and coconut oil groups weighed significantly more than those in the control group (P = .004). Mean Neonatal Skin Condition Score scores were significantly higher in the control group at 1 month postdischarge than in the sunflower seed oil and coconut oil groups (P = .000). CONCLUSIONS Skin care with coconut oil and sunflower seed oil was effective in maintaining the skin integrity and weight of neonates at 1 month postdischarge. The authors recommend that randomized controlled trials on skin care in preterm infants using sunflower, coconut, and other oils be conducted with larger sample sizes.

Impact of Coconut Oil and Its Bioactive Metabolites in Alzheimer's Disease and Dementia: A Systematic Review and Meta-Analysis.

Background/Objectives: Alzheimer's disease (AD) is the most common form of dementia and affects approximately 50 million individuals worldwide. Interest in coconut oil (CO) as a potential dietary intervention has surged owing to its substantial medium-chain triglyceride (MCT) content. Therefore, sustaining cognitive function and potentially slowing the progression of AD are crucial. This systematic review and meta-analysis evaluated the effects of CO and its bioactive metabolites on AD and dementia. Methods: The review protocol is registered in PROSPERO (CRD42023450435). Relevant research articles published between January 2015 and June 2023 were systematically searched. Seven studies met the predetermined eligibility criteria. Thematic analysis was utilized to synthesis the data about the qualitative features, while meta-analysis was employed for the quantitative findings. A meta-analysis was conducted to assess the standardized mean difference (SMD) and the corresponding 95% confidence interval (CI). Forest plots were generated using Review Manager 5.3 (RevMan 5.3). Results: The analysis revealed that all studies showed consistent results regarding the effects of CO on cognitive scores, with little variability in the true effects of CO on cognitive scores across the studies included in the meta-analysis. Conclusions: CO improved cognitive scores in patients with AD compared with those in the control group (p < 0.05). The results of this study add to the increasing amount of evidence indicating that MCTs found in CO might be a way to improve abilities and potentially slow the advancement of AD. The findings of this study may encourage the development of targeted dietary strategies and interventions for individuals at risk of or diagnosed with AD.

Captivating Combustion Traits of Bio-Oil Droplets Enriched with Bio-Additives from the Areca Shell Waste

Fuel derived from crude vegetable oil, such as coconut oil, holds promise as an alternative energy source to mitigate the increasing reliance on fossil fuels driven by population growth and industrial activities. The experiment involved suspending a single droplet of crude coconut oil mixed with activated carbon from areca shell waste and placed at the junction on R-type thermocouple (Pt/Pt-Rh13%). The droplets were ignited using a hot wire and subjected to atmospheric pressure and room temperature. Coconut oil comprises a saturated triglyceride carbon chain compound of approximately 91%, and areca shell waste possesses a porous structure that fosters favorable interactions between fuel molecules. The droplet combustion method was selected to streamline the process and enhance the contact area between air and fuel, thereby boosting the reactivity of fuel molecules. The research found that adding activated carbon shortens the carbon chain, making it more reactive and easier for the fuel to ignite. Specifically, activated carbon significantly enhances fuel performance at a concentration of two parts per million (ppm). At this level, the fuel absorbs heat more effectively and ignites faster compared to one ppm and three ppm levels. Moreover, the results show that heat absorption occurs slowly at one ppm, while at three ppm, the increased molecular mass of the fuel can strengthen carbon-bonding forces. These factors contribute to a longer ignition time for the fuel. The findings suggest that the activated carbon from areca shell waste can play a good role as a combustion catalyst, where overall, fuel performance increases.

Characterization and evaluation of physicochemical properties, phytochemicals and antimicrobial analysis of coconut oil

Characterization and evaluation of physicochemical properties, phytochemicals and antimicrobial analysis of coconut oil

CLINICAL EVALUATION OF COCONUT OIL PULLING PRACTICE VERSUS CURCUMIN BASED MOUTHWASH IN PLAQUE INDUCED GINGIVITIS: AN INTERVENTIONAL STUDY

Introduction:Periodontitis is an inflammatory disease caused by a complex mix of microbes. Effective prevention and management of periodontal disease depend on the regular and thorough removal of bacterial plaque. There are various mechanical and chemical methods available for plaque control. Historically, essential oils have been utilized to treat numerous oral conditions, and many have been studied for their anti-plaque and anti-gingivitis properties. However, the use of coconut oil for these purposes has been explored in only a few studies. Objectives:The current study was conducted to compare and assess the therapeutic efficacy of Coconut Oil-Pulling and Curcumin-based Mouthwash in patients with plaque and gingivitis. Additionally, we measured the IL-1β levels in the saliva of patients with gingivitis. Materials and Method:A total of 40 periodontally healthy volunteers with the age range of 20-55 years were randomly divided into two groups: ‑Group 1: virgin coconut oil mouthrinse (Anveshan Coconut oil) and Group 2: Curcumin mouthrinse(Turmix Mouthwash). Clinical parameters such as Plaque Index (PI), Gingival Index (GI), and Gingival Bleeding Index (GBI) were recorded for all the subjects. An unstimulated salivary sample was collected at baseline and after 1 month for estimation of IL-1β levels. Results:Curcumin mouthwash was determined to be more effective when compared to coconut oil pulling in cases of mild gingivitis. Conclusion:Both the study groups showed reductions in PI, GI,GBI scores and IL-1β levels. It was observed that Coconut oil pulling and Curcumin mouthwash both served as a good alternative but curcumin mouthwash was found to be more effective when compared to coconut oil pulling in cases of mild gingivitis.

UMUR SIMPAN TELUR AYAM YANG DILAPISI DENGAN KAPUR (CaCO3) DAN MINYAK KELAPA

Preservation of fresh eggs can be done by coating the centipede with coating material. The purpose of this study was to determine the physical quality of chicken eggs treated with centipede coating and shelf life at room temperature. The ingredients used are purebred chicken eggs, coconut oil and chalk. The parameters observed are: Egg weight, egg index, egg white index, yolk index, egg white pH, yolk pH, egg color. The study design is a complete randomized design of factorial patterns. The results showed that the type of egg centipede coating material and shelf life showed a marked difference in egg weight. The coating material and shelf life have no effect on the egg index. The treatment of centipede coating material has a very noticeable effect on the egg white index and egg yolk index. The treatment of centipede coating material and shelf life have a significant influence on the pH value of egg white and egg yolk pH. The color of the yolk of purebred chickens with the treatment of centipede coating materials and the length of storage at room temperature has a noticeable effect on the color of the yolk. The conclusion that the coating of the egg centipede using chalk and coconut oil and shelf life showed a marked difference in egg weight, egg white index, yolk index, white pH, yolk pH and egg color, while the egg index did not differ markedly.

Phytochemical Content towards Weak Antioxidants Activity of Traditional Fermented VCO as the Basic Ingredient for Ear Drops

Chronic Suppurative Media Otitis is caused by pathogenic bacteria, which, if the growth is not controlled, will cause meningitis and can lead to death. Virgin Coconut Oil is an oil made through the traditional fermentation process of coconut milk and can inhibit the growth of pathogenic bacteria that cause Chronic Suppurative Media Otitis. So far, pathogenic bacteria can be controlled using ear drops made from artificial chemicals, which can cause resistance. Only a few have studied the manufacture of natural-based ear drops from VCO. The purpose of this study is to study the potential of VCO as a natural essential ingredient for ear drops for patients with OMSK by analyzing its antioxidant content using the DPPH method and its phytochemical content using Ultraviolet-Visible Spectroscopy (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Its antioxidants in the IC50 (ppm) region are 583.92mg/L. The types of fatty acids contained in VCO consist of Medium-Chain Fatty Acid (MCFA), Long-Chain Fatty Acid (LCFA) and Very Long Chain Fatty Acid (VLCFA), the most of which is lauric acid (MCFA), as much as 49.28%. Conventional analysis of secondary metabolites showed the presence of Alkaloids, Terpenoids, Ascorbic Acid, and Saponins. It can be concluded that the chemical content of Virgin Coconut Oil made from fermentation can be used in the health field as a primary ingredient for making ear drops for CSOM patients.

Microemulsion-based topical hydrogels containing lemongrass leaf essential oil (Cymbopogon citratus (DC.) Stapf) and mango seed kernel extract (Mangifera indica Linn) for acne treatment: Preparation and in-vitro evaluations.

Current treatments for severe acne include combinations of synthetic anti-inflammatory and antibacterial drugs, which possess numerous side effects. Therefore, this study developed microemulsion-based hydrogel containing lemongrass leaf essential oil (Cymbopogon citratus (DC.) Stapf) and mango seed kernel extract (Mangifera indica Linn) as a potential natural therapy for inflammatory acne. To this end, the microemulsions were first prepared using pseudo-ternary phase diagrams with soybean oil and coconut oil, cremophor RH40, and PEG 400. The optimal formula could load 1% lemongrass oil and 10% mango extract, possessed a spherical droplet size of ~18.98 nm, a zeta potential of -5.56 mV, and a thermodynamic stability. Secondly, the microemulsion-based hydrogel was developed by simple mixing the optimal microemulsion in carbopol-940 hydrogel (3.5% w/w). The product showed a viscosity of ~3728 cPs, a pH of 5.4-6.2, a spreadability of ~24 cm, an in-vitro Franz-cell cumulative release rate of ~80% for polyphenol content and ~60% for citral within 12 h, and a good physicochemical stability of > 3 months. Thirdly, the skin compatibility/irritability of the microemulsion-based hydrogel was determined by the HET-CAM assay, which showed non-irritation level. Finally, the anti-inflammatory activities of the hydrogel, using heat-induced BSA denaturation assay and LPS-stimulated RAW 264.7 NO inhibition assay, was 4-times higher than that of the reference drug Klenzit-C® (adapalene and clindamycin gel). Moreover, the hydrogel possessed strong anti-biofilm activity in Cutibacterium acnes, comparable with Klenzit-C®. Conclusively, the microemulsion-based hydrogel containing lemongrass oil and mango seed extract demonstrated much potentials to be a promising natural drug for acne treatment.

FATTY ACID COMPOSITION OF DIFFERENT COMMERCIALLY SOLD VEGETABLE OILS WITHIN SOUTHERN KADUNA, KADUNA STATE, NIGERIA

Most of the edible vegetable oils in southern Kaduna are locally processed and are available in a large commercial quantity with little known about their fatty acid (FA) content. The aim of this work was to investigate the fatty acid content of these oils and their potential impact on human health. Six commonly used edible vegetable oil samples sold in various Southern Kaduna markets were analyzed for different fatty acids content using gas chromatography-mass spectrometry (GC-MS). The result of the analysis showed differences in the contents of saturated fatty acids (SFAs), monounsaturated (MUFAs), and polyunsaturated (PUFAs). Coconut oil and kernel oil showed high content of SFAs (86.21% and 78.76%) respectively, while olive oil and peanut oil displayed significant amounts of MUFAs (75.40% and 47.62%) respectively due to high content of oleic acid (C18:1). Soybean oil with 58.06% of linoleic acid (C18:2) showed the highest total PUFA content of 65.23%. The Health Risk Index (HRI) was evaluated to assessed the impact associated with the overall intake of fatty acids in vegetable oils. The HRI were 1.78 (peanut), 0.04 (kernel), 0.23 (palm), 0.2(coconut), 4.05(soybean) and olive (0.71). Thus, soybean oil, peanut oil and olive oil have the optimum PUFA:SFA ratio according to FAO/WHO recommendations. Hence, they are recommended for mass consumption. The health promotion index (HPI) was also evaluated to assessed the content of certain essential fatty acids in various oils which have specific health benefits. The results of HPI investigation showed that only Soybean oil contains the balanced ω-6: ω-3 fatty acid ratio of 8.1 than others.

Alleviating Active Labor Pain: The Impact of Virgin Coconut Oil (VCO) Back Massage During the First Stage of Labor

Labor pain is one of the most intense sensations experienced by women during childbirth, particularly during the first stage of labor. This study aimed to evaluate the effect of back massage with Virgin Coconut Oil (VCO) on the intensity of active labor pain during the first stage. The research employed a quantitative, pre-experimental one-group pretest-posttest design, involving 18 mothers in active phase labor at IMP Restu Bunda, West Tulang Bawang Regency. Pain levels were measured using the Visual Analog Scale (VAS) before and after the back massage with VCO. Results showed that the average labor pain intensity before the intervention was 7.0, and after the back massage with VCO, the average pain decreased to 5.2. Statistical analysis using the Wilcoxon test revealed a significant reduction in pain intensity (p-value = 0.000), indicating that back massage with VCO effectively reduced labor pain. Seventeen respondents reported a decrease in pain, while only one respondent experienced no change. The findings suggest that back massage with VCO is a viable non-pharmacological method for managing labor pain, with minimal side effects.

RANCANG BANGUN MESIN PEMURNIAN VIRGIN COCONUT OIL (VCO) DENGAN METODE SENTRIFUGAL BERBASIS INTERNET OF THGINGS (IoT)

Virgin Coconut Oil (VCO) is a virgin coconut oil produced from the extraction process of fresh coconut meat without the use of chemicals or high temperatures. VCO has high economic value with various health benefits. The process of making VCO involves a centrifugal machine to separate the oil from coconut milk. However, the control of the VCO Purification machine still relies on manual intervention by the operator, which can lead to human error and affect production efficiency. In order to improve efficiency and reduce the risk of human error, Internet of Things (IoT) and Mobile Application technologies can be applied in the control of VCO Purification machines. The programming language used is PHP because it can be accessed on various platforms, especially Mobile. The sensor used is an IR (Infrared) speed sensor for measuring rotational speed. The Mobile Application can be a convenient interface for operators to control and record the operation history of the VCO purification machine. This research has succeeded in developing an IoT and Mobile Application-based control system that is effective in controlling VCO purification machines, increasing production efficiency, and reducing the risk of human error.

The Effect Of Virginia Coconut Oil (VCO) On The Prevention Of Decubitus In Prolonged Bed Rest Patients

Decubitus is a condition where external pressure causes damage or injury to local tissue. Trauma and surgical wounds can cause damage to skin integrity, long-term pressure on the skin can also cause irritation and the formation of decubitus, People who experience nerve damage due to stroke, traumatic injury, diabetes mellitus, or coma are also more likely to experience decubitus. The purpose of this study was to determine the effect of administering virgin coconut oil (VCO) on the risk of decubitus. The research method used the Pre Experiment design method with a one group pre post test design. The population in this study were all patients in the Akasia Inpatient Room, Siti Fatimah Az-Zahra Hospital, South Sumatra Province. The total number of patients treated was 30 respondents. Sampling was carried out using the total sampling technique. The results of the study showed that there was an effect of administering virginia coconut oil on the risk of decubitus in patients with long-term bed rest, the significant value of the non-parametric statistical test (Wilcoxon) was 0.000 (p value 0.000 &lt;0.05). Suggestions are expected as input and sources of information in providing counseling to patients who are bedridden for a long time to use pharmacological therapy in preventing decubitus, and routinely use Virginia Coconut Oil which has many benefits.

Perspective on the health effects of unsaturated fatty acids and commonly consumed plant oils high in unsaturated fat.

Epidemiological and clinical trial evidence indicates that n-6 polyunsaturated fatty acid (PUFA) intake is cardioprotective. Nevertheless, claims that n-6 PUFA intake promotes inflammation and oxidative stress prevail. This narrative review aims to provide health professionals with an up-to-date evidence overview to provide the requisite background to address patient/client concerns about oils containing predominantly unsaturated fatty acids (UFA), including MUFA and PUFA. Edible plant oils, commonly termed vegetable oils, are derived from vegetables, nuts, seeds, fruits and cereal grains. Substantial variation exists in the fatty acid composition of these oils; however, all are high in UFA, while being relatively low in saturated fatty acids (SFA), except for tropical oils. Epidemiological evidence indicates that higher PUFA intake is associated with lower risk of incident CVD and type 2 diabetes mellitus (T2DM). Additionally, replacement of SFA with PUFA is associated with reduced risk of CVD and T2DM. Clinical trials show higher intake of UFA from plant sources improves major CVD risk factors, including reducing levels of atherogenic lipids and lipoproteins. Importantly, clinical trials show that increased n-6 PUFA (linoleic acid) intake does not increase markers of inflammation or oxidative stress. Evidence-based guidelines from authoritative health and scientific organisations recommend intake of non-tropical vegetable oils, which contain MUFA and n-6 PUFA, as part of healthful dietary patterns. Specifically, vegetable oils rich in UFA should be consumed instead of rich sources of SFA, including butter, tallow, lard, palm and coconut oils.

Performance enhancement, life cycle assessment, and feature analysis of wheat starch-based NaCl-binder as a sustainable alternative to OPC mortar

The primary aim of this investigation is to advance the properties of an innovative and sustainable wheat starch-based NaCl-binder (WSB) material. The scope encompasses the material's ecological and structural applications and potential for paving usage. The creation of hydrophobic and environmentally friendly WSB samples under microwave curing conditions was achieved by using a salt mortar with varying sizes of salt grains, water, and wheat starch as a binding agent. This study investigated the effects of curing time, water and starch amounts, and salt particle size on strength, modulus of rupture, and modulus of elasticity. Additionally, XRD, XRF, and SEM analyses were carried out to assess the chemical characteristics of WSB and the bonding between its particles. The mix design with the highest compressive strength, which consisted of 1 wt% starch, 8 min of microwave treatment, and 10 wt% water, attained a strength of 25.27 MPa. Furthermore, coconut oil was employed to effectively make the samples water-resistant. Following different guidelines, WSB could be categorized as structural concrete and utilized for structural and paving bricks. A vital aspect of this study is the exceptional environmental performance of WSB as a structural material, highlighting its originality and importance.

Formulation &amp; Evaluation of Hair Growth Serum from Hibiscus Flowers and Leaves

The need for cosmetics is very high in this fast-paced environment. The pharmaceutical industry's use of cosmetics is growing daily. We use cosmetics on a daily basis. Cosmetics are meant to be used on teeth, nails, hair, and skincare. Note that toothpastes, hair oils, and hair dyes are all considered cosmetics that are used by everyone on a daily basis. Because using synthetic or chemical products might have unfavorable side effects, individuals are increasingly gravitating toward organic, natural, and herbal formulations that have negligible or no side effects. Herbal remedies are typically recognized for having "No side effect." People deal with a variety of issues, including premature greying of the hair, split ends, dandruff, hair thinning, and excessive sebum production. People are therefore searching for strategies to improve hair growth, prevention, and maintenance. The present investigation focuses at the preparation and evaluation of a herbal hair growth serum made with a combination of natural substances, including olive oil, peppermint oil, vitamin E, hibiscus flowers and leaves, Amla Powder, and curry leaves. The purpose of the serum is to improve scalp health, strengthen hair follicles, and promote hair growth. Hibiscus flowers and leaves are used to extract bioactive chemicals, which are then infused with curry leaves and Amla powder in olive oil. For added advantages, vitamin E and lavender oil are added. Hibiscus flowers are rich in amino acid, which is prime building block of keratin. The leaves also reduce the growth of dandruff-causing fungi, reduce dandruff flakes and prevent its recurrence. Amla Powder has antimicrobial and anti-inflammatory properties that can help reduce dandruff. Curry leaves are rich in antioxidants and proteins, these antioxidants neutralize the free radicals and keep hair healthy and strong. Coconut oil absorbs into your hair quickly, providing moisture to tame frizz and heal breakage. The effectiveness of the serum is assessed in the study using variables including hair growth rate, thickness, strength, scalp health, and general health of the hair. Preliminary findings point to encouraging results in terms of strengthening hair growth and enhancing hair quality.

Characterization of quality parameters and phytosterol content in oils and their formulated margarines

AbstractUnlike lipid stability and oxidation studies in commonly used edible oils and margarines, margarines formulated with unconventional oils are not well characterized. This study investigated the effect of heat treatment (HT) on the stability and content of phytosterol in njangsa seed oil (NSO), bush mango oil (BMO), soybean oil (SBO), coconut oil (CCO), and margarines formulated from their blends: BN (BMO and NSO), BS (BMO and SBO), CN (CCO and NSO), CS (CCO and SBO), and commercial margarines (CM1 and CM2). Both oils and margarines were heat‐treated at 130, 170, and 210°C for 10, 15, 20, and 120 min (only oils). Changes in free fatty acid (FFA), peroxide value (PV), para‐anisidine value (AnV), and fatty acid (FA) composition and phytosterol content were determined after 20 min (margarines) and 120 min (oils) of HT and compared to their control/pre‐HT/unheated (UH) counterparts. The FA composition did not change significantly with HT. Polyunsaturated fatty acids (PUFA)‐rich oils such as NSO and SBO showed significantly higher increase in FFA content with HT compared with oils with higher saturated fatty acid content (BMO and CCO). Oils with higher proportions of linoleic acid, such as SBO (68.3%) and NSO (60.35%), had higher AnV at the end of the HT compared with oils with lower content, such as BMO (0.51%). Phytosterol content fluctuated with HT, and changes in content were generally more pronounced in β‐sitosterol than in stigmasterol and campesterol. Both principal component analysis (PCA) and partial least‐squares discriminant analysis (PLS‐DA) were carried out to observe possible clusters. The results suggest that changes in quality and content of oils and margarines during heating are dependent on more than their fatty acid composition.

Enhanced lipase production and characterization from Aeromonas media VBC8: Applications in biodegradation of lubricating oil waste

This study aimed to explore the potential of a novel indigenous strain for the improved production of lipase from castor oil-contaminated soil. Among the various isolates, Aeromonas media VBC8 was found to be the most effective for lipase production. The effect of different inducer oils (olive, peanut, soybean, rice bran, sunflower, coconut, sesame, and fish liver oil) on the biomass of A. media VBC8 and its lipase activity was determined. Among the various oils assessed, fish liver oil exhibited highest lipase activity, with 89 U/mL with 9.1 g/L of biomass. Furthermore, Box-Behnken Design was used to optimize the cultural conditions resulting in an enhanced lipase activity of 1156 U/mL. The lipase was purified through ammonium salt (60 w/v %) precipitation, desalting and ion exchange column, achieving a yield of 16 % and specific activity of 98.4 U/mL. The purified lipase remained active over a wide range of pH 4.0–11.0 and temperature of 10–80 °C with maximum activity at pH 8.0 and 40 °C. SDS-PAGE analysis revealed the lipase's molecular weight to be 94 kDa. The study also evaluated the role of crude and purified lipase in the biodegradability of lubricating oil waste, achieving a maximum fatty acid conversion of 39 and 76 %, respectively, after 7 h incubation at room temperature.