Differential regulation of key virulence factors by SbfR in Vibrio parahaemolyticus.

Combined histopathological, immunoenzymatic and transcriptomic analyses reveal the immune response mechanisms of silver pomfret infected by Vibrio parahaemolyticus.

RPA-CRISPR/Cas12a-coupled microfluidic biosensor enabling on-site, sensitive quantification of Vibrio parahaemolyticus.

Correlation of Elastic Light Scatter pattern analysis of clinical and shellfish strains of Vibrio parahaemolyticus from New Zealand with virulence characteristics.

Profiles of gut microbiome in Litopenaeus vannamei artificially infected with Vibrio parahaemolyticus causing translucent post-larva disease.

Whole-genome insights into antibiotic resistance in Vibrio strains isolated from shrimp farms in Mexico.

Exploratory analysis of epigenetic dynamics and small extracellular vesicles in Penaeus vannamei hemocytes under inactivated Vibrio parahaemolyticus priming.

Proteomic analysis of the hepatopancreas in Litopenaeus vannamei artificially infected by Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease

Vietnamese herbal extracts exhibit potent antibacterial activity against Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in shrimp aquaculture.

Structural interactions and transcriptional regulation by caffeic acid against multidrug-resistant Vibrio species: Mechanistic insights and efficacy in shrimp.

Genome-wide association study for Vibrio parahaemolyticus resistance in the swimming crab Portunus trituberculatus

Vibrio parahaemolyticus infection in Manila clams (Ruditapes philippinarum): A case study of microbiome perturbation.

CRISPR/Cas12a empowered electrochemical biosensor for ultrasensitive detection of Vibrio parahaemolyticus in seafood samples.

Vibrio parahaemolyticus persisters: Novel insights into stress resilience, metabolic reprogramming, and implications for food safety

Bolstering shrimp resilience against AHPND: Biofloc reduce Vibrio parahaemolyticus population, affects microbial composition, and increase Penaeus vannamei shrimp growth and resistance

Immunostimulation of Pacific white shrimp (Penaeus vannamei) through dietary NagroWall enhances resistance to Vibrio parahaemolyticus infection.

Design, synthesis, and antibacterial activity evaluation of tryptanthrin derivatives.

Characterization of novel Vibrio parahaemolyticus JD2305 and comparative genomics of the Tc toxin mobilome in Vibrio pathogens associated with translucent postlarvae disease

Biofloc-based bacterial community forms an ecological barrier for resisting Vibrio parahaemolyticus infection in shrimp

The pathogenic bacterium Vibrio parahaemolyticus represents a substantial economic and public health concern; however, elucidating its virulence mechanisms has been significantly impeded by its inherent resistant to genetic manipulation, primarily attributed to sophisticated immune defense systems including restriction-modification (R-M) modules, CRISPR-Cas systems, standalone DNases, and DdmDE systems. Paradoxically, while genetic modification is essential for overcoming these barriers, the very barriers themselves obstruct DNA introduction. Our investigation focused on the V. parahaemolyticus X1 strain, where initial plasmid transformation attempts proved unsuccessful. However, low-efficiency conjugation allowed knockout of defense genes, thereby silencing the host's defense mechanisms. Our findings revealed a standalone DNase, Vpn, as the predominant obstacle to foreign DNA entry in the X1 strain, while a DdmDE system executes elimination of invaded plasmids. Leveraging these insights, we created the V. parahaemolyticus X2 strain via sequential depletion of the Vpn nuclease and the DdmDE system. Capitalizing on the bacterium's exceptional growth rate, characterized by a generation time of approximately 10.5 min, we established a highly efficient molecular cloning platform capable of creating a new plasmid construct within a single day. This work not only presents a strategic framework for genetic manipulation of previously recalcitrant bacterial species but also underscores the potential of fast-growing marine bacteria as promising candidates for next-generation biotechnological applications.