Vastus Lateralis Fascicle Research Articles

Residual force enhancement decreases when scaling from the single muscle fiber to joint level in humans

BackgroundResidual force enhancement (rFE), defined as increased isometric force following active lengthening compared to a fixed-end isometric contraction at the same muscle length and level of activation, is present across all scales of muscle. While rFE is always present at the cellular level, often rFE “non-responders” are observed during joint-level voluntary contractions. MethodsWe compared rFE between the joint level and single fiber level (vastus lateralis biopsies) in 16 young males. In vivo voluntary knee-extensor rFE was measured by comparing steady-state isometric torque between a stretch-hold (maximal activation at 150°, stretch to 70°, hold) and a fixed-end isometric contraction, with ultrasonographic recording of vastus lateralis fascicle length (FL). Fixed-end contractions were performed at 67.5°, 70.0°, 72.5°, and 75.0°; the joint angle that most closely matched FL of the stretch-hold contraction's isometric steady-state was used to calculate rFE. The starting and ending FLs of the stretch-hold contraction were expressed as % optimal FL, determined via torque-angle relationship. ResultsIn single fiber experiments, the starting and ending fiber lengths were matched relative to optimal length determined from in vivo testing, yielding an average sarcomere excursion of ∼2.2–3.4µm. There was a greater magnitude of rFE at the single fiber (∼20%) than joint level (∼5%) (p = 0.004), with “non-responders” only observed at the joint level. ConclusionBy comparing rFE across scales within the same participants, we show the development of the rFE non-responder phenomenon is upstream of rFE's cellular mechanisms, with rFE only lost rather than gained when scaling from single fibers to the joint level.

In vivo vastus lateralis fascicle excursion during speed skating imitation

Mechanical power is a key performance indicator in long track speed skating. Maximal power output in athletic performance can be achieved when mechanical properties of muscles, such as the force-length relationship, are optimized. The purpose of this study was to determine the in vivo operating range of vastus lateralis (VL) fascicle lengths during speed skating imitation and compare the fascicle lengths to those that define the VL force-length relationship. Sixteen sub-elite long track speed skaters (7 females and 9 males; body mass: 72.5 [11.5] kg; age: 22.1 [2.7] years) performed maximal voluntary isometric knee extensions at nine different knee joint positions (20–120°) on the left leg to obtain the maximal vastus lateralis (VL) force-length relationship. Participants then performed a speed skating imitation exercise, the turn-cable, at three progressive perceived efforts (50%, 75%, 100%) to identify the VL fascicle excursion during a complete imitation skating stroke. Fascicle lengths and knee joint angles were examined at initial-contact, peak EMG, and take-off. Fascicles between initial contact and peak EMG covered the descending limb of both the maximal and submaximal force-length relationships while operating over the plateau region from peak EMG to take-off. We conclude that the VL works at sub-optimal length during the gliding phase of skating, but at optimal length for maximal force production during the crucial push-off phase where propulsion is provided.

The effects of crank power and cadence on muscle fascicle shortening velocities, muscle activations and joint-powers during cycling.

Whilst people typically chose to locomote in most economical fashion, during bicycling they will, unusually, chose cadences that are higher than metabolically optimal. Empirical measurements of the intrinsic contractile properties of the vastus lateralis (VL) muscle during submaximal cycling suggest that the cadences that people self-selected (SSC) might allow for optimal muscle fascicle shortening velocity for the production of knee extensor muscle power. It remains unclear, however, whether this is consistent across different power outputs where the SSC varies. We examined the effect of cadence and external power requirements on muscle neuromechanics and joint powers during cycling. VL fascicle shortening velocities, muscle activations and joint-specific powers were measured during cycling between 60 and 120rpm (including SSC), while participants produced 10%, 30%, and 50% of peak maximal power. VL shortening velocity increased as cadence increased but was similar across the different power outputs. Although no differences were found in the distribution of joint powers across cadence conditions, the absolute knee joint power increased with increasing crank power output. Muscle fascicle shortening velocities increased in VL at the SSC as pedal power demands increased from submaximal towards maximal cycling. A secondary analysis of muscle activation patterns showed minimized activation of VL and other muscles near the SSC at the 10% and 30% power conditions. Minimization of activation with progressively increasing fascicle shortening velocities at the SSC may be consistent with the theory that the optimum shortening velocity for maximizing power increases with intensity of exercise and recruitment of fast twitch fibers.

Linking muscle mechanics to the metabolic cost of human hopping.

Many models have been developed to predict metabolic energy expenditure based on biomechanical proxies of muscle function. However, current models may only perform well for select forms of locomotion, not only because the models are rarely rigorously tested across subtle and broad changes in locomotor task but also because previous research has not adequately characterised different forms of locomotion to account for the potential variability in muscle function and thus metabolic energy expenditure. To help to address the latter point, the present study imposed frequency and height constraints to hopping and quantified gross metabolic power as well as the activation requirements of medial gastrocnemius (MG), lateral gastrocnemius (GL), soleus (SOL), tibialis anterior (TA), vastus lateralis (VL), rectus femoris (RF) and biceps femoris (BF), and the work requirements of GL, SOL and VL. Gross metabolic power increased with a decrease in hop frequency and increase in hop height. There was no hop frequency or hop height effect on the mean electromyography (EMG) data of ankle musculature; however, the mean EMG of VL and RF increased with a decrease in hop frequency and that of BF increased with an increase in hop height. With a reduction in hop frequency, GL, SOL and VL fascicle shortening, fascicle shortening velocity and fascicle to MTU shortening ratio increased, whereas with an increase in hop height, only SOL fascicle shortening velocity increased. Therefore, within the constraints that we imposed, decreases in hop frequency and increases in hop height resulted in increases in metabolic power that could be explained by increases in the activation requirements of knee musculature and/or increases in the work requirements of both knee and ankle musculature.

Age-related alterations in human motor units properties and in muscle innervation profile

Introduction: Mounting evidence suggests that motoneuron and neuromuscular junction (NMJ) degeneration, muscle fiber denervation and loss of motor units contribute to muscle wasting and weakness in old age. However, these aspects are still poorly investigated in humans. This study aimed at comparing the neuromuscular system integrity and function of young and older adults, with particular focus on NMJ and motor unit potential (MUP) characteristics. We hypothesized that older individuals would present altered electrophysiological properties and innervation profile. Methods: We recruited 55 healthy young individuals (YI) (aged 26.1±4.5 yr; 50% females) and 88 older individuals (OI) (aged 75.9±4.7 yr; 55% females). Muscle force was evaluated by isometric dynamometry, muscle morphology by ultrasound and neuromuscular excitability by compound motor unit action potential (CMAP). MUP properties and motor unit number estimate (iMUNE) were obtained by intramuscular electromyography (iEMG). In a sub-cohort of individuals (n=16 YI and n=34 OI), vastus lateralis (VL) muscle biopsies were obtained. We assessed denervation status by scoring neural cell adhesion molecule (NCAM) positive fibers. Generalized linear mixed models were carried out for iEMG data, while unpaired t-tests were carried out for all the other parameters. Results: OI showed alterations in muscle morphology when compared to YI, evident as smaller whole quadriceps cross-sectional area (p<0.001) and VL fiber diameter (p<0.01), together with smaller VL pennation angles (p<0.001) and fascicle lengths (p<0.05), resulting in an increased ultrasound sarcopenia index (p=0.001). Muscle force (p<0.001) and CMAP (p<0.001) were also largely impaired in OI, suggesting that muscle function and neuromuscular excitability are considerably affected by aging. iEMG analysis revealed changes in MUP turns (p<0.001), near fiber (NF) MUP duration (p<0.001) and NF count (p<0.001) in OI, pointing towards an increased MUP complexity, in absence of sex differences. The iMUNE was reduced (p<0.001) and the NMJ transmission stability was significantly altered in OI, as suggested by elevated NF jiggle (p<0.001) and segmented jitter (p<0.05). These electrophysiological alterations seem likely due to altered muscle innervation profile, as highlighted by an increased percentage of NCAM-positive fiber (p<0.001). Conclusions: This study showed that changes in muscle morphology and function, MUP properties, motor units loss and denervation status occur in older humans; these may be serve as useful markers of human neuromuscular aging. The present work was funded by PRIN project NeuAge (2017CBF8NJ_001) to MVN and the Milky Way Foundation, the Baxter Foundation, and the Li Ka Shing Foundation to HMB. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Quadriceps Muscle Morphology Is an Important Determinant of Maximal Isometric and Crank Torques of Cyclists.

The aim of this study was to determine if quadriceps morphology [muscle volume (MV); cross-sectional area (CSA)], vastus lateralis (VL) muscle architecture, and muscle quality [echo intensity (ECHO)] can explain differences in knee extensor maximal voluntary isometric contraction (MVIC), crank torque (CT) and time-to-exhaustion (TTE) in trained cyclists. Twenty male competitive cyclists performed a maximal incremental ramp to determine their maximal power output (POMAX). Muscle morphology (MV; CSA), muscle architecture of VL and muscle quality (ECHO) of both quadriceps muscles were assessed. Subsequently, cyclists performed three MVICs of both knee extensor muscles and finally performed a TTE test at POMAX with CT measurement during TTE. Stepwise multiple regression results revealed right quadriceps MV determined right MVIC (31%) and CT (33%). Left MV determined CT (24%); and left VL fascicle length (VL-FL) determined MVIC (64%). However, quadriceps morphological variables do not explain differences in TTE. No significant differences were observed between left and right quadriceps muscle morphology (p > 0.05). The findings emphasize that quadriceps MV is an important determinant of knee extensor MVIC and CT but does not explain differences in TTE at POMAX. Furthermore, quadriceps morphological variables were similar between the left and right quadriceps in competitive cyclists.

Non-Invasive estimation of muscle fiber type using ultra Sonography

Introduction: The aim of this study was to estimate the muscle fiber type i.e., fast/white muscle fibers and slow/red muscle fibers) on the basis of selected muscle architectural parameters i.e., Pen-nation angle, Muscle thickness and Fascicle Length of Rectus Femoris and Vastus Lateralis using ultrasound image analysis. Materials and Methods: In this work of investigation, a total sample comprised of 30 highly trained male athletes of Lakshmibai National Institute of Physical Education Gwalior, Madhya Pradesh was considered as sample for present investigation. Purposive sampling technique was employed for selecting sample, out of which 15 were marathon runners and 15 were 100-meter sprinters. The selected subjects’ age ranged between 19 to 25 years. Muscle architectural parameters such as Pen-nation angle, Fascicle length, and Muscle thickness were measured using a B-Mode ultrasonography method (Model-WIPRO GE VOLUSON E 6). Results: The developed estimate model explains 54.6% to 72.8% of the variation in muscle fiber type. Thus, the logistic regression equation developed was: - Log p/(1-p) = 10.955 - 3.278 (VL_FL) + 1.267 (VL_PA). Conclusion: The wald statistics obtained from the logistic model revealed that decreasing the vastus lateralis pen nation angle by one unit increases the likelihood of having white muscle fiber by 2.54 times, while increasing the Vastus Lateralis Fascicle Length by one unit increases the likelihood of having white muscle fiber by 0.62 times. In order to determine muscle fiber type on the basis of muscle architectural parameters Vastus lateralis pen-nation angle and fascicle length were the most discriminating variables among selected fiber types.

Quadriceps Muscle Action and Association With Knee Joint Biomechanics in Individuals with Anterior Cruciate Ligament Reconstruction.

Insufficient quadriceps force production and altered knee joint biomechanics after anterior cruciate ligament reconstruction (ACLR) may contribute to a heightened risk of osteoarthritis. Quadriceps muscle lengthening dynamics affect force production and knee joint loading; however, no study to our knowledge has quantified in vivo quadriceps dynamics during walking in individuals with ACLR or examined correlations with joint biomechanics. Our purpose was to quantify bilateral vastus lateralis (VL) fascicle length change and the association thereof with gait biomechanics during weight acceptance in individuals with ACLR. The authors hypothesized that ACLR limbs would exhibit more fascicle lengthening than contralateral limbs. The authors also hypothesized that ACLR limbs would exhibit positive correlations between VL fascicle lengthening and knee joint biomechanics during weight acceptance in walking. The authors quantified VL contractile dynamics via cine B-mode ultrasound imaging in 18 individuals with ACLR walking on an instrumented treadmill. In partial support of our hypothesis, ACLR limb VL fascicles activated without length change on average during weight acceptance while fascicle length on the contralateral limb decreased on average. The authors found a positive association between fascicle lengthening and increase in knee extensor moments in both limbs. Our results suggest that examining quadriceps muscle dynamics may elucidate underlying mechanisms relevant to osteoarthritis.

Walking speed does not affect net vastus lateralis fascicle length change on average during weight acceptance

Faster walking speeds increase the demand on quadriceps muscles to produce adequate force to decelerate body mass and control knee flexion. Quadriceps fascicle behavior (i.e., fascicle length changes) influences force generation, which in turn affects mechanical loading of the articular cartilage during walking and the biochemical environment of the knee joint. The fascicle behavior underlying different walking speeds remains unclear but should be characterized to better understand how the quadriceps muscles accommodate faster walking speeds, speeds that often associate with better cartilage health outcomes. Our purpose was to quantify quadriceps muscle net fascicle length change during weight acceptance across a range of walking speeds in the context of more well-documented changes in muscle activity and knee joint moments. We hypothesized that vastus lateralis (VL) fascicles in healthy young adults would produce force with more overall lengthening in early stance at faster walking speeds with concomitant increases in muscle–tendon unit (MTU) lengthening, internal peak knee extensor moment (pKEM), vertical ground reaction force (GRF), and muscle activity. Participants walked for two-minute trials at their preferred speed and at 0.75 m/s and 1.75 m/s. We found that on average, the VL accommodates the greater mechanical demands of walking at faster speeds with greater muscle activity and while resisting muscle lengthening behavior. We infer then that tendon stretch accommodates MTU lengthening in healthy young adults across a range of speeds and suggest these results motivate additional studies aimed at evaluating VL fascicle behavior individuals with known quadriceps strength deficits, inhibition, or heightened risk for developing osteoarthritis.

Differences in Rate of Force Development, Muscle Morphology and Maximum Strength between Weightlifters and Track and Field Throwers

The purpose of this study was to investigate the differences between weightlifters and track and field throwers in terms of the rate of force development (RFD), lean mass, muscle architecture and one-repetition maximum (1-RM) muscle strength. Sixteen elite male athletes (eight weightlifters and eight track and field throwers) participated in the study. Measurements were performed one week after the annual national championships and included anthropometry, body composition analysis (dual X-ray absorptiometry), vastus lateralis (VL) muscle architecture (ultrasonography), countermovement jump (CMJ), leg press isometric RFD and 1-RM strength in snatch and back squat. RFD was similar between groups (p > 0.05), although RFD relative to lean mass was higher for weightlifters (p < 0.05). Throwers had a higher trunk lean mass compared to weightlifters (p = 0.007). Weightlifters had a longer VL fascicle length compared to throwers (p = 0.037). Performances in CMJ height, CMJ power relative to body mass and 1-RM strength were higher for the weightlifters (p < 0.05). Lean body mass index was correlated with back squat (r = 0.667) and snatch (r = 0.498). VL fascicle length was significantly correlated with snatch (r = 0.631) and back squat (r = 0.718). These results suggest that weightlifters may produce greater power outputs and 1-RM strength than track and field throwers, which may be associated with longer VL fascicle length.

Altered countermovement jump force profile and muscle-tendon unit kinematics following combined ballistic training.

Combined heavy‐ and light‐load ballistic training is often employed in high‐performance sport to improve athletic performance and is accompanied by adaptations in muscle architecture. However, little is known about how training affects muscle‐tendon unit (MTU) kinematics during the execution of a sport‐specific skill (e.g., jumping), which could improve our understanding of how training improves athletic performance. The aim of this study was to investigate vastus lateralis (VL) MTU kinematics during a countermovement jump (CMJ) following combined ballistic training. Eighteen young, healthy males completed a 10‐week program consisting of weightlifting derivatives, plyometrics, and ballistic tasks under a range of loads. Ultrasonography of VL and force plate measurements during a CMJ were taken at baseline, mid‐test, and post‐test. The training program improved CMJ height by 11 ± 13%. During the CMJ, VL's MTU and series elastic element (SEE) length changes and velocities increased from baseline to post‐test, but VL's fascicle length change and velocity did not significantly change. It is speculated that altered lower limb coordination and increased force output of the lower limb muscles during the CMJ allowed more energy to be stored within VL's SEE. This may have contributed to enhanced VL MTU work during the propulsion phase and an improved CMJ performance following combined ballistic training.

Age-related differences of vastus lateralis muscle morphology, contractile properties, upper body grip strength and lower extremity functional capability in healthy adults aged 18 to 70 years

BackgroundThere is a lack of of cross-sectional research that has investigated muscle morphology, function, and functional capability in all age-bands of healthy adults. The primary aim of this study was to evaluate age-related differences in indices of vastus lateralis (VL) muscle morphology, function and functional capability in a sample of healthy males and females aged 18-70yrs. Secondary aims were to evaluate relationships between age and VL muscle morphology and function and functional capability.MethodsB mode Ultrasonography and Tensiomyography were used to measure VL muscle thickness, pennation angle, fascicle length, and contractile properties in 274 healthy adults aged 18-70yrs. Measurements of grip strength and functional capability (1-min chair rise test) were also taken. Data analysis included descriptive statistics, correlations, one-way ANOVAs, and multiple regressions.ResultsNegative correlations were found between age and muscle thickness (rs = -.56), pennation angle (rs = -.50), fascicle length (rs = -.30), maximal displacement (rs = -.24), grip strength (rs = -.27) and the 1-min chair rise test (rs = -.32). Positive correlations were observed between age and the echo intensity of the muscle (rs = .40) and total contraction time (rs = .20). Differences in the indices of muscle health were noticeable between the 18–29 age band and the 50–59 and 60–70 age bands (p < 0.05). The interaction of age and level of physical activity predicted changes in the variables (r2 = .04—.32).ConclusionAge-related differences in muscle health are noticeable at 50 years of age, and age-related differences are larger in females compared to males. It was suggested that the thickness of the VL changed the most with age across the adult lifespan and that physical activity likely acts to abate detrimental change.

Residual force enhancement is affected more by quadriceps muscle length than stretch amplitude.

Little is known about how muscle length affects residual force enhancement (rFE) in humans. We therefore investigated rFE at short, long, and very long muscle lengths within the human quadriceps and patellar tendon (PT) using conventional dynamometry with motion capture (rFETQ) and a new, non-invasive shear-wave tensiometry technique (rFEWS). Eleven healthy male participants performed submaximal (50% max.) EMG-matched fixed-end reference and stretch-hold contractions across these muscle lengths while muscle fascicle length changes of the vastus lateralis (VL) were captured using B-mode ultrasound. We found significant rFETQ at long (7±5%) and very long (12±8%), but not short (2±5%) muscle lengths, whereas rFEWS was only significant at the very long (38±27%), but not short (8±12%) or long (6±10%) muscle lengths. We also found significant relationships between VL fascicle length and rFETQ (r=0.63, p=0.001) and rFEWS (r=0.52, p=0.017), but relationships were not significant between VL fascicle stretch amplitude and rFETQ (r=0.33, p=0.126) or rFEWS (r=0.29, p=0.201). Squared PT shear-wave-speed-angle relationships did not agree with estimated PT force-angle relationships, which indicates that estimating PT loads from shear-wave tensiometry might be inaccurate. We conclude that increasing muscle length rather than stretch amplitude contributes more to rFE during submaximal voluntary contractions of the human quadriceps.

Effect of the Knee and Hip Angles on Knee Extensor Torque: Neural, Architectural, and Mechanical Considerations.

This study examined the influence of knee extensors’ hip and knee angle on force production capacity and their neuromuscular and architectural consequences. Sixteen healthy men performed sub-maximal and maximal voluntary isometric contractions (MVIC) of knee extensors with four different combinations of the knee and hip angles. Muscle architecture, excitation-contraction coupling process, muscular activity, and corticospinal excitability were evaluated on the vastus lateralis (VL) and rectus femoris (RF) muscles. MVIC and evoked peak twitch (Pt) torques of knee extensors increased significantly (p < 0.05) by 42 ± 12% and 47 ± 16% on average, respectively, under knee flexed positions (110° flexion, 0° = full extension) compared to knee extended positions (20° flexion) but were not different between hip positions (i.e., 0° or 60° flexion). Knee flexion also affected VL and RF muscle and fascicle lengths toward greater length than under knee extended position, while pennation angle decreased for both muscles with knee flexion. Pennation angles of the VL muscle were also lower under extended hip positions. Alternatively, no change in maximal muscle activation or corticospinal activity occurred for the VL and RF muscles across the different positions. Altogether these findings evidenced that MVIC torque of knee extensors depended particularly upon peripheral contractile elements, such as VL and RF muscle and fascicle lengths, but was unaffected by central factors (i.e., muscle activation). Furthermore, the hip position can affect the pennation angle of the VL, while VL muscle length can affect the pennation angle of the RF muscle. These elements suggest that the VL and RF muscles exert a mutual influence on their architecture, probably related to the rectus-vastus aponeurosis.

Suppressed quadriceps fascicle behavior is present in the surgical limbs of those with a history of ACL reconstruction

The balance of published data have largely focused on adaptations in muscle and fiber size after anterior cruciate ligament reconstruction (ACLR), failing to account for the dynamic changes in the behavior of the muscles’ contractile elements that strongly contribute to force production. To better understand the sources of quadriceps dysfunction, the purpose of our research was to determine if alterations in fascicle behavior are present after ACLR. Unilateral ACLR individuals (9 m/9f; 21 ± 3 yrs; 1.74 ± 0.12 m;71.58 ± 13.31 kg; months from surgery:38 ± 36) and healthy controls (3 m/6f; 23 ± 2 yrs; 1.67 ± 0.10 m; 63.51 ± 10.11 kg) participated. In-vivo vastus lateralis fascicle behavior was recorded using ultrasonography during three maximal isokinetic knee extensions (60°·s−1). Fascicle length, angle, and shortening velocity were calculated and analyzed from rest to peak torque. Peak knee extension torque was averaged between isokinetic trials (Nm·kg−1). Group by limb interactions were assessed using separate two-way analyses of variance and were further evaluated by comparing 95% confidence intervals where appropriate. Significant interactions were present for fascicle angle at peak torque (P = 0.01), fascicle length excursion (P = 0.05), fascicle angle excursion (P < 0.01), fascicle shortening velocity (P = 0.05) and strength (P = 0.03). Upon post-hoc evaluation, the surgical limb displayed altered in-vivo fascicle behavior compared to all limbs (P < 0.05) and reduced strength compared to the contralateral and right control limbs (P < 0.05). No other significant interactions were present (P > 0.05). Our data show that those with a history of ACLR have fascicles that are slower, lengthen less and operate with lower angles relative to the axis of force production. Altered fascicle behavior after ACLR may be an important underlying factor to explaining the protracted quadriceps dysfunction.

Architectural anatomy of the quadriceps and the relationship with muscle strength: An observational study utilising real-time ultrasound in healthy adults.

Quadriceps atrophy and morphological change is a known phenomenon that can impact significantly on strength and functional performance in patients with acute or chronic presentations conditions. Real-time ultrasound (RTUS) imaging is a noninvasive valid and reliable method of quantifying quadriceps muscle anatomy and architecture. To date, there is a paucity of normative data on the architectural properties of superficial and deep components of the quadriceps muscle group to inform assessment and evaluation of intervention programs. The aims of this study were to (1) quantify the anatomical architectural properties of the quadriceps muscle group (rectus femoris, vastus intermedius, and vastus lateralis) using RTUS in healthy older adults and (2) to determine the relationship between RTUS muscle parameters and measures of quadriceps muscle strength. Thirty middle aged to older males and females (age range 55-79years; mean age=59.9±7.08years) were recruited. Quadriceps muscle thickness, cross-sectional area, pennation angle, and echogenicity were measured using RTUS. Quadriceps strength was measured using hand-held dynamometry. For the RTUS-derived quadriceps morphological data, rectus femoris mean results; circumference 9.3cm; CSA 4.6cm2 ; thickness 1.5cm; echogenicity 100.2 pixels. Vastus intermedius mean results; thickness 1.8cm; echogenicity 99.1 pixels. Vastus lateralis thickness 1.9cm; pennation angle 17.3°; fascicle length 7.0cm. Quadriceps force was significantly correlated only with rectus femoris circumference (r=0.48, p=0.007), RF echogenicity (r=0.38, p=0.037), VI echogenicity (r=0.43, p=0.018), and VL fascicle length (r=0.43, p=0.019). Quadriceps force was best predicted by a three-variable model (adjusted R2 =0.46, p<0.001) which included rectus femoris echogenicity (B=0.43, p=0.005), vastus lateralis fascicle length (B=0.33, p=0.025) and rectus femoris circumference (B=0.31, p=0.041). Thus respectively, rectus femoris echogenicity explains 43%, vastus lateralis fascicle length explains 33% and rectus femoris circumference explains 31% of the variance of quadriceps force. The study findings suggest that RTUS measures were reliable and further research is warranted to establish whether these could be used as surrogate measures for quadriceps strength in adults to inform exercise and rehabilitation programs.

Effects of a 25-Week Periodized Training Macrocycle on Muscle Strength, Power, Muscle Architecture, and Performance in Well-Trained Track and Field Throwers.

Anousaki, E, Zaras, N, Stasinaki A-N, Panidi, I, Terzis, T, and Karampatsos, G. Effects of a 25-week periodized training macrocycle on muscle strength, power, muscle architecture, and performance in well-trained track and field throwers. J Strength Cond Res 35(10): 2728-2736, 2021-The purpose of the study was to investigate the effect of a 25-week macrocycle on strength, power, vastus lateralis (VL) muscle architecture, and competitive track and field throwing performance, in well-trained track and field throwers. Twelve well-trained throwers (age: 24.3 ± 5.5 years, mass: 96.6 ± 9.9 kg, and height: 1.82 ± 0.02 m), participated in the study. All athletes followed a 25-week periodized training program divided into 3 training phases: the hypertrophy/maximum strength phase, the maximum strength/power phase, and the power/speed phase. Measurements were performed at the beginning of the training period (T1), after the first training phase (T2), and after the end of the training period (T3). Measurements included the following: competitive track and field throwing performance; shot put throws; maximum strength (1-RM) in snatch, clean, and squat; countermovement jump (CMJ); and VL muscle architecture. Competitive track and field throwing performance and shot put throws increased from T1 to T3 by 10.9 ± 3.2% (p = 0.001) and 5.1 ± 6.4% (P = 0.049), respectively. 1 RM strength in snatch and squat increased significantly from T1 to T3 by 9.7 ± 8.9% (p = 0.001) and 9.9 ± 7.1% (p = 0.002), respectively. Countermovement jump height increased only from T1 to T2 by 10.9 ± 11.8% (p = 0.026). A significant increase was found for VL fascicle length between T1 and T3 (9.6 ± 11.1%, p = 0.049). Strong correlations were found between the percentage increase of competitive track and field throwing performance with 1 RM snatch (r = 0.571, p = 0.046) and with shot put throws (r = 0.715, p = 0.001). Twenty five weeks of training may increase performance and VL fascicle length, whereas increases in 1 RM snatch and in shot put throws are associated with competitive track and field throwing performance in well-trained throwers.

Seven months of aerobic intradialytic exercise training can prevent muscle loss in haemodialysis patients: an ultrasonography study.

This study aimed to evaluate the effects of 7-month intradialytic exercise training (IDE) on vastus lateralis (VL) muscle architecture, functional capacity and quality of life in stable haemodialysis (HD) patients. 44 HD patients were randomly assigned into an Exercise Group (EG = 21), which they received 7 months of IET, at 60% of pre-assessed maximum aerobic power, 3/week) or into a Control Group (CG = 23, no exercise). VL architecture was evaluated with ultrasonography, functional capacity was assessed using a series of functional tests (6min walking test, 5 repetitions sit-to-stand, sit-to-stand 60s, handgrip strength), and maximal aerobic power determined with a modified version of the Åstrand test. Quality of life and fatigue levels were evaluated using validated questionnaires. VL fascicle angle and length did not change significantly in both groups (P > 0.05). Muscle thickness decreased in CG (P = 0.02) while it was fully preserved in the EG. Functional capacity increased only in the EG (6min walking test 15.79 ± 12.35%, P < 0.001; max aerobic power 26.36 ± 1.24%, P < 0.001; STS-60rep: 10.23 ± 1.60%, P < 0.000; hand grip strength: 5.14 ± 28.30%, P < 0.02). Intradialytic aerobic exercise training, improves functional capacity and prevents thigh muscle mass loss in HD patients. It is evident that muscle ultrasonography could play a pivotal role in assessing muscle quality changes in haemodialysis patients. A higher level of training intensity or combination with resistance exercises may be required to further improve anabolism and influence muscle architecture in this patient population. NCT03905551.

Faster intrinsic rate of torque development in elbow flexors than knee extensors: Effect of muscle architecture?

We studied the effect of pennate vs. fusiform muscle architecture on the rate of torque development (RTD) by examining the predominately fusiform elbow flexors (EF) and highly-pennate knee extensors (KE). Seventeen male volunteers (28.4 ± 6.2 years) performed explosive isometric EF and KE contractions (MVCs). Biceps brachii and vastus lateralis fascicle angles were measured to confirm their architecture, and both the rate of voluntary muscle activation (root-mean-square EMG in the 50 ms before contraction onset; EMG-50) and electromechanical delay (EMD; depicting muscle-tendon series elasticity) were assessed as control variables to account for their influence on RTD. MVC torque, early (RTD50) and late (RTD200) RTDs were calculated and expressed as absolute and normalized values. Absolute MVC torque (+412%), RTD50 (+215%), and RTD200 (+427%) were significantly (p < 0.001) higher in KE than EF. However, EF RTD50 was faster (+178%) than KE after normalization (p = 0.02). EMG-50 and EMD did not differ between muscle groups. The results suggest that the faster absolute RTD in KE is largely associated with its higher maximal torque capacity, however in the absence of differences in rates of muscle activation, fiber type, and EMD the fusiform architecture of EF may be considered a factor allowing its faster early RTD relative to strength capacity.

Force–Time Characteristics of Dynamic and Isometric Muscle Actions: Association with Muscle Architecture in Female Athletes

The association between force–time characteristics of isometric leg press (ILP) and countermovement jump (CMJ) with vastus lateralis (VL) muscle architecture, was examined in 19 female athletes (aged 23.2 ± 5.4 years). Peak force (PF), average rate of force development (ARFD) and rate of force development (RFD) at different time epochs were calculated from the force–time curve, as well as CMJ jump height and power. Significant correlations were found between ILP-PF and CMJ power (r = 0.658, p &lt; 0.01), while both variables were correlated with VL thickness and fascicle length (r = 0.471 to 0.648, p &lt; 0.05). Significant correlations were also observed between ILP-RFD epochs and VL fascicle length (r = 0.565 to 0.646, p &lt; 0.05) and between CMJ height with VL thickness (r = 0.523, p &lt; 0.05). Furthermore, positive correlations were found between ILP and CMJ in ARFD (r = 0.625, p &lt; 0.01) and RFD epochs (r = 0.464 to 0.566, p &lt; 0.05). ILP-PF and muscle thickness accounted for 52.8% (p = 0.002) of the variance in CMJ power. These results suggest that isometric force time characteristics are associated with power generation during dynamic muscle actions. Furthermore, VL muscle thickness and fascicle length are associated with rapid force production in female athletes, irrespective of the type of muscle action.