Values Of Blood Indices Research Articles

Spatiotemporal distribution and bionomics of Anopheles stephensi in different eco-epidemiological settings in Ethiopia

BackgroundMalaria is a major public health concern in Ethiopia, and its incidence could worsen with the spread of the invasive mosquito species Anopheles stephensi in the country. This study aimed to provide updates on the distribution of An. stephensi and likely household exposure in Ethiopia.MethodsEntomological surveillance was performed in 26 urban settings in Ethiopia from 2021 to 2023. A kilometer-by-kilometer quadrant was established per town, and approximately 20 structures per quadrant were surveyed every 3 months. Additional extensive sampling was conducted in 50 randomly selected structures in four urban centers in 2022 and 2023 to assess households’ exposure to An. stephensi. Prokopack aspirators and CDC light traps were used to collect adult mosquitoes, and standard dippers were used to collect immature stages. The collected mosquitoes were identified to species level by morphological keys and molecular methods. PCR assays were used to assess Plasmodium infection and mosquito blood meal source.ResultsCatches of adult An. stephensi were generally low (mean: 0.15 per trap), with eight positive sites among the 26 surveyed. This mosquito species was reported for the first time in Assosa, western Ethiopia. Anopheles stephensi was the predominant species in four of the eight positive sites, accounting for 75–100% relative abundance of the adult Anopheles catches. Household-level exposure, defined as the percentage of households with a peridomestic presence of An. stephensi, ranged from 18% in Metehara to 30% in Danan. Anopheles arabiensis was the predominant species in 20 of the 26 sites, accounting for 42.9–100% of the Anopheles catches. Bovine blood index, ovine blood index and human blood index values were 69.2%, 32.3% and 24.6%, respectively, for An. stephensi, and 65.4%, 46.7% and 35.8%, respectively, for An. arabiensis. None of the 197 An. stephensi mosquitoes assayed tested positive for Plasmodium sporozoite, while of the 1434 An. arabiensis mosquitoes assayed, 62 were positive for Plasmodium (10 for P. falciparum and 52 for P. vivax).ConclusionsThis study shows that the geographical range of An. stephensi has expanded to western Ethiopia. Strongly zoophagic behavior coupled with low adult catches might explain the absence of Plasmodium infection. The level of household exposure to An. stephensi in this study varied across positive sites. Further research is needed to better understand the bionomics and contribution of An. stephensi to malaria transmission.Graphical

The Relationship between Complement Components C1R and C5 Gene Polymorphism and the Values of Blood Indices in Suckling Piglets.

The main mechanism of innate immunity is the complement system. Its components include the protein products of the C1R and C5 genes, which are involved in the classical activation pathway as well as the inflammatory and cytolytic immune responses, respectively. The aim of this study was to determine the relationship between PCR-restriction fragment length polymorphism in C1R (726T > C) and C5 (1044A > C) genes, and the values of hematological and biochemical blood indices in suckling crossbred (Polish Large White × Polish Landrace × Duroc × Pietrain) piglets (n = 473), considering their age (younger, 21 ± 3 days, n = 274; older, 35 ± 3 days, n = 199) and health status. The frequencies of the C5 genotypes deviated from the Hardy-Weinberg expectations. Younger piglets, healthy piglets, piglets that deviated from physiological norms and older piglets with the C1R TT genotype all had lower white and red blood cell indices. In piglets with the C5 CC genotype, younger piglets, piglets that deviated from physiological norms and older piglets, a greater number and/or percentage of monocytes were recorded in the blood. Older piglets also showed an increase in the number of leukocytes and granulocytes, along with a tendency for a decrease in the percentage of lymphocytes in their blood. We concluded that a polymorphism in the C1R gene may exhibit a functional association or genetic linkage with other genes involved in the process of erythropoiesis. Furthermore the relationship between the C5 gene polymorphism and the number and/or percentage of monocytes in the blood may modify the body's defense abilities. Piglets with the CC genotype, having an increased number/proportion of these cells in their blood, probably display a weakened immune response to pathogens or a chronic stimulation of the immune system.

Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system.

Acute liver failure (ALF) is a severe liver disease with high morbidity and mortality rates. Animal models are important for research on ALF. This study aimed to establish a reproducible, Tibetan miniature pig model of D-galactosamine-induced ALF and verify it using a dual plasma molecular adsorption system (DPMAS). Tibet miniature pigs were randomly divided into four groups (A, B, C, D) after catheterization. D-galactosamine (D-gal) at 0.45, 0.40, 0.35, and 0.35 g/kg body weight, respectively, was injected through the catheter. Group D was treated with DPMAS 48 h after D-gal administration. Vital signs and blood index values were recorded every 12 h after D-gal administration. H&E, TUNEL, Ki67, and Masson staining tests were performed. After D-gal administration, Tibetan miniature pigs developed different degrees of debilitation, loss of appetite, and jaundice. Survival times of groups A, B, C, and D were 39.7 ± 5.9, 53.0 ± 12.5,61.3 ± 8.1, and 61 ± 7 h, respectively. Blood levels of ALT, AST, TBIL, ammonia, PT, and inflammation factors significantly increased compared with baseline levels in the different groups (Ps < 0.05). Pathological results revealed a clear liver cell necrosis positive correlation with D-gal dose. However, DPMAS did not increase the survival time in ALF, ammonia, or liver cell necrosis. We successfully established a reproducible Tibetan miniature pig model of d-galactosamine-induced ALF, and we believe that a dosage of 0.35 g/kg is optimal.

ИНТЕГРАЛЬНЫЕ ГЕМАТОЛОГИЧЕСКИЕ ИНДЕКСЫ НЕСПЕЦИФИЧЕСКОЙ РЕЗИСТЕНТНОСТИ СВИНЕЙ КАК КРИТЕРИЙ ИХ ПРОДУКТИВНЫХ КАЧЕСТВ В УСЛОВИЯХ ДЕЙСТВИЯ ТЕХНОЛОГИЧЕСКИХ СТРЕСС-ФАКТОРОВ

The research was carried out on a purebred livestock of the main sows of the Poltava meat breed. In the blood, the amount of leukocyte formula was determined and, on the basis of the primary data, integral leukocyte indices were calculated, characterizing the nonspecific resistance of the organism. Approximate reference values of blood indices were established in the population of healthy sows of the Poltava meat breed, in which technological stress led to the development of the stage of resistance. The main load under the action of technological stress in pigs is experienced by the agranulocytic link in the blood, especially monocytes. No reliable interrelationships between blood indices and productivity indicators were established, however, a direct dependence of some indices on other links of the body's resistance was revealed.

Effect of food domestication on the growth of Elopichthys bambusa

Elopichthys bambusa is an economically important freshwater fish with high nutritional value. In this study, we compared domesticated and wild fish at the physiological, biochemical, histological, and molecular levels to better understand the changes that occur in farmed fish in order to provide ideas about how to optimize the artificial feed formulation used in aquaculture of this species. Water, ash, and crude protein contents did not differ significantly between the two groups. The methionine and glutamic acid contents were 20% and 13.4% higher in the domesticated group than that in the wild group, respectively. Twenty-three types of fatty acids were detected in the wild group compared to 11 in the domesticated group. The iron content in the domesticated group was higher than that in the wild group. The blood index values did not differ significantly between the two groups, except for alanine aminotransferase (ALT), total protein (TP), and globulin (GLO) contents. The ALT content in the wild group was significantly higher and the TP and GLO contents were significantly lower than those in the domesticated group. Histologically, significant differences in the numbers of folds, villi, and goblet cells were detected between the two groups. The expression of t1r1 and t1r3 in the intestines and gills of the domesticated group was significantly lower than that of the wild group. This study provides an important theoretical basis for the reproduction and breeding studies and the quality identification of domesticated Elopichthys bambusa. It will promote the development of large-scale farming of Elopichthys bambusa.

Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice.

Ferroptosis is a non-apoptotic form of cell death that relies on iron and lipid peroxidation, which is associated with multiple pathological processes in several diseases. Erastin is a small molecule capable of initiating ferroptotic cell death in cancer cells, which has shown great potential for cancer therapy. However, the physiological and pathological role of erastin-induced ferroptosis on healthy tissues has not been well characterized. The present study intraperitoneally injected erastin into healthy mice to detect the metabolic changes of several tissues of mice. Erastin injection induced typical characteristics of ferroptosis with higher level of serum iron and malondialdehyde and lower level of glutathione and glutathione peroxidase 4 protein. Erastin injection enhanced iron deposition in the brain, duodenum, kidney and spleen of mice. Erastin-induced ferroptosis altered the blood index values, causing mild cerebral infarction of brain and enlarged glomerular volume of kidney. It also promoted the growth of duodenal epithelium with thicker, longer and denser villi in erastin-treated mice. The findings provided evidence that erastin induced ferroptosis and caused pathological changes in healthy tissues of mice. This suggested that the anti-tumor drug erastin was somewhat toxic to healthy tissues.

Short communication: Comparative study of Hematological variation in healthy and fungal infected Kalabans, Bangana dero (Hamilton, 1822)

An attempt has been done to explore the hematological variation in healthy and saprolegnia infected minor carp, Bangana dero. Hematological parameters i.e total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were examined in healthy and infected fish of wild and cultured stock. Except TLC, which significantly increased in the infected fish, all parameters decreased in response to infection in wild as well as cultured stock. Decreases in Hb, TEC were significant, indicating that saprolegniosis causes anemia and immunosuppression. Wild stock also has non significant slightly higher values of blood indices except the TLC, showed better health and immunity of wild stock over the cultured stock. The values and trend of the examined parameters are indicators of mycosis in fish and would be used as tool for diagnosis of fungal infected fish.

Study of anemia in diabetic and non-diabetic subjects: A hospital-based study in Lucknow, Uttar Pradesh

Background: India is expected to be the Diabetic Capital of World in the coming years. Alarmingly by 2045, it is expected that 130 million Indians will be having diabetes. It alters the physiology of the human body and the associated renal insufficiency, inflammation, and oxidative stress further complicate the disease. Anemia is expected to be present in 11–65% of diabetics. Anemia in diabetics thus requires investigation and cannot be neglected. Aims and Objectives: The present study was undertaken to study the anemia in diabetic and non-diabetic subjects. Materials and Methods: The present study included 92 subjects divided into diabetic (n = 48) and non-diabetic (n = 44) groups. Hemoglobin (Hb) and blood indices (mean corpuscular volume [MCV], mean corpuscular Hb [MCH], and MCHC concentration [MCHC]) were compared and further evaluation was done on the basis of the severity of anemia (as per the WHO classification). Statistical unpaired t-test was employed for data analysis. P < 0.05 was taken as statistically significant. Results: Among the diabetic group out of 48 subjects, mild, moderate, and severe anemia was present in 8 (Hb in g/dl was 11.25 ± 0.26), 11 (9.73 ± 0.83 g/dl Hb), and 4 (6.13 ± 1.15 g/dl Hb) subjects, respectively. In non-diabetic subjects, mild and moderate anemia was present in 5 (11.42 ± 0.33 g/dl Hb) and 7 (9.98 ± 0.83 g/dl Hb) subjects, respectively. Thus, in diabetics, the percentage of those having moderate anemia was more as compared to non-diabetics. In diabetics out of 48 subjects, 27 were female (56.25%) and 21 (43.75%) were male. Similarly, in non-diabetics, 28 (63.64%) were female and 16 (36.36%) were male. In the present study, the mean ± SD age (years) of diabetics and non-diabetics was found to be 44.17 ± 8.82 and 41.6 ± 8.80, respectively. The difference in mean ± SD values of Hb was found to be insignificant with P = 0.167. Similarly, insignificant differences were found between the mean ± SD values of blood indices, namely, MCV (P = 1.795), MCH (P = 0.897), and MCHC (P = 1.484), respectively, between diabetics and non-diabetics. Conclusions: On the basis of the study, it may be concluded that female gender and the elderly are prone to anemia and thus are expected to be more prone to complications due to anemia in diabetes. The duration of diabetes is expected to influence the results and thus requires inclusion in the study design.

Relationship between RNASE1, ANG and RNASE6 gene polymorphism and the values of blood indices in suckling piglets.

The relationship between PcR-restriction fragment length polymorphism in RNASE1 (296 A/G), ANG (149 G/T) and RNASE6 (389 C/T) genes and the values of haematological and biochemical blood indices was analysed in crossbred suckling piglets (n = 473), aged 21 ± 3 days (younger, n = 274) and 35 ± 3 days (older, n = 199), descending from Polish Large White × Polish Landrace sows and Duroc × Pietrain boars. The observed distribution of all genotypes was consistent with the Hardy-Weinberg equilibrium. Anaemia was more common in younger piglets with RNASE1 GA genotype but in the blood of older GA piglets a higher count and percentage of granulocytes were noted. This could be related to the destruction of erythrocytes in younger piglets and enhanced host defence in older ones. ANG gene polymorphism was associated with the severity of iron deficiency in younger piglets. This is supposed to be linked with the different ability to protect immune cells against suppression and degradation during iron deficiency. in older piglets, this mutation differentiated the reactivity of the immune system. Varying levels of iron status and red blood cell indices in RNASE6 genotypes presumably resulted from the coupling of genes involved in iron metabolism and expressed in an age-dependent manner.

Evaluation the Ozone Treatment To Control the Infection of Saprolegniasis in Cyprinus carpio L.

The objective of present study is to set up an alternative method of controlling of saprolegniasis, in Cyprinus carpio L. using ozone. For this purpose, six different treatments were applied of 120 fish (20 fish/treatment), of which three infected with Saprolegnia spp. (2×104 zoospors/l) and were treated with ozone (O3) in concentrations of 0.25, 0.50 and 0.75 mg l−1 (1hour), one reference treatment with formalin (0.15 ml l−1, 30 min), two treatments one as positive control (infected with Saprolegnia spp. without disinfectants) and one as negative control without any disinfectants. After 14 days of treatment with ozone and formalin, blood samples were collected from fish for hematological, biochemical and Immunological tests. Survival rate was also studied. Considerable changes have been recorded in the mean values of blood indices. RBCs count and Hemoglobin content, were found significantly increased (p<0.05) in all ozone treatments compared to C- group. Total protein and globulin were significantly increased (p<0.05) in all ozone and formalin treatments in comparison to positive and negative groups (C- and C+). Albumin showed a significant decrease (p<0.05) in all ozone and formalin treatments relative to C+ and C-group. The results of respiratory burst activity (measured by Nitro blue tetrazolium reduction assay) showed significantly different (p<0.05) in all ozone and formalin treatments respectively compared to control groups (C+ and C-); Highest respiratory activity was observed in ozone treatment at 0.50 mg/l. Among Ozone treatments 0.50 mg l−1 showed the highest survival rate (90%) followed by formalin treatment (80%). Survival rate of the control group (without disinfectant) was 20% and there was a significant difference (P<0.05) with other treatments. It was obvious that the obtained results were highly suggestive for the efficiency of ozone as an efficient antifungal disinfectant for fish.

Assessment of liver function in pregnant anemic women upon oral iron and folic acid supplementation

Oral iron therapy is the most widely prescribed treatment for iron deficiency anemia. However, oral iron supplementation may also lead to various health problems. The recognition of these physiological variations is essential for the diagnosis of liver diseases during the course of pregnancy. Therefore, the objective of this study was to assess the variations in levels of routine liver function tests (LFTs) in pregnant women before and after iron and folic acid treatment. Iron and folic acid was supplemented to 500 normal pregnant anemic women (mild=200, moderate=200 and severe=100) and 100 age matched normal pregnant non-anemic as controls daily for 100 days. Blood index values and liver function parameters were precisely monitored. Hemoglobin (Hb), total protein (TP), iron (Fe), albumin and alkaline phosphatase (ALP) levels were found increased (P<0.001; P<0.01; P<0.05) after treatment in mild, moderate, severe and control, respectively. Lipid peroxidation (LPx), aspartate transaminase (AST) and alanine transaminase (ALT) were increased in pretreated mild, moderate and severe groups and further increased after all treated subjects. Moreover, gamma-glutamyl transpeptidase (GGT) was found to decrease in pre and posttreated subjects. Treatment with iron and folic acid although has remarkable efficacy for Hb and body iron stores although for the cost of increasing the associated compartment of total bilirubin, AST and ALT concomitant with decreased GGT levels. Data obtained from the present study provide new insights into the mandatory application of liver function tests likely to be monitored at regular and specific intervals during the course of pregnancy.

Study on Impact of Iron and Folic Acid on the Plasma Trace Minerals in Pregnant Anemic Women.

Iron deficiency anemia is one of the causes that lead to significant mortality and morbidity among pregnant women and fetus. The present study was undertaken to explore oral iron supplementation can modify the metal contents in pregnant anemic women. Iron and folic acid supplementations was given to 500 anemic women (mild=200, moderate=200, and severe=100) and 100 age matched non-anemic controls daily for 100days. Blood index values and plasma trace minerals were estimated as per standard protocols. Haemoglobin and ferritin levels were found significantly increased (p<0.001) in anemic and control subjects after treatment. Moreover, the serum transferring receptor levels and total iron binding capacity were found significantly decreased in all treated groups. Iron (Fe), zinc (Zn) and copper (Cu) levels were found increased (p<0.01) after oral iron supplementation groups. Moreover, selenium (Se) manganese (Mn) and were found to be decreased in all treated groups. Data provides the conclusion that iron and folic acid supplementation recovered the essential trace minerals, except manganese, which may lead to various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus.

Haematological evaluation of sodium fluoride toxicity in oryctolagus cunniculus.

Blood is promptly affected by environmental pollutants and toxicants that can cause many metabolic disorders. The high level of fluoride acts as a potential pollutant, insecticide and rodenticide with very high toxicity, associated with the hematological damage. This study aimed to determine the toxicity of Sodium Fluoride on hematological parameters in Oryctolagus cunniculus. Twenty rabbits were acclimatized and divided in to control group and three experimental groups.Experimental group-I, II and III were treated with 10, 30 and 50 mg/kg body weight doses of Sodium Fluoride orally. Various blood parameters such as TEC, Hb, HCT, MCV, MCH, MCHC, TLC and PLT count were investigated. Result findings showed that values of blood indices in experimental groups were significantly lower than the control group. Oneway ANOVA was applied for statistical analysis. The outcomes of the current studies indicated the reduction in RBC counts (anemia), leukocyte count (leukocytopenia), monocytosis, eosinopenia, neutrophilia and thrombocytosis on fluoride intoxication. Hematological disruptions like microcytic hypochromic anemia and decreased leukocyte count may be linked to the inflammatory effects of Sodium Fluoride on lymphatic organs.

STUDY OF DHATU SARATA AND ITS ASSOCIATION WITH BLOOD INDICES

Ashta Sarata parikshana is explained especially to determine the strength of person. It is important to add modern parameter to giv e authenticity to the ancient subjective parameter . 50 healthy male students between the ages of 18 - 25 years were selected for study . Sarata parikshan was done with the help of standard sarata parikshan proforma speci ally prepared by AYUSOFT C - DAC Centre for Development of Advance Computing Pune , India to find out percentage of each dhatu separately. Blood indices hav e been formulated by modern science to detect type of anaemia by extracting information about structure, morphology of Red Blood Cells and about haemoglobin. Blood indices give complete information of Red Blood Cells. Blood sample were collected for analys is of blood indices and this was done by auto analyser . This was s urvey type co relational research . The s tatistical analysis shows that “ there is a relation between Rakta sarata and Blood indices” , it’s a positive relation. The person having higher percen tage of Ra kta sarata possesses good value of Blood indices .

Influence of Sex on the Hematological and Morphometric Parameters of Cyprinus Carpio (Linnaeus, 1758) from Shkodra Lake

The aim of the presents study was to obtain the influence of sex on some parameters of Cyprinus carpio (Linnaeus, 1758). The study has been carried out during spawning migration in the period March 2011 - May 2011 and involved 60 healthy individuals of fish, randomly collected. The specimens were weighed and measured. Blood samples were taken to determine hematocrit, hemoglobin, erythrocyte count and erythrocyte indices: mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. The mean values of blood indices and morphometric parameters were determinate annually for male and female. Comparison of blood parameters was performed according to sex differences. The results of this study showed that male fish have higher values of hemoglobin, hematocrit, and erythrocyte count, than female. Statistical analysis revealed that differences in hematological and morphometric parameters between males and females were significant. DOI: 10.5901/ajis.2013.v2n8p45

Safety and persistence of orally administered human Lactobacillus sp. strains in healthy adults

The aim of the study was to evaluate the safety and persistence of selected Lactobacillus strains in the gastrointestinal tract (GIT) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. In the first phase of the study, nine individuals consumed capsules containing Lactobacillus gasseri 177 and E16B7, Lactobacillus acidophilus 821-3, Lactobacillus paracasei 317 and Lactobacillus fermentum 338-1-1 (each daily dose 1×1010 cfu) for 5 consecutive days. Data on gut health, blood parameters, and liver and kidney function were collected. The persistence of Lactobacillus strains was assessed by culturing combined with arbitrarily primed polymerase chain reaction (AP-PCR) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) on days 0, 5, 8, 10 and 20 from faecal samples. All strains survived gastrointestinal passage and were detected on the 5th day. L. acidophilus 821-3 was detected in four volunteers on the 8th day (4.3 to 7.0 log10 cfu/g) and in two on the 10th day (8.3 and 3.9 log10 cfu/g, respectively). In the second phase of the study, five additional volunteers consumed L. acidophilus 821-3 (daily 1×1010 cfu) for 5 consecutive days. The strain was subsequently detected in faeces of all individuals using real-time PCR on the 10th day (range 4.6-6.7; median 6.0 log10 cell/g) in both phases of the study for at least 5 days after discontinuation of consumption. The administration of high doses of different Lactobacillus strains did not result in any severe adverse effects in GIT and/or abnormal values of blood indices. Thus, the strain L. acidophilus 821-3 is a promising candidate for probiotic and biotechnological applications. Further studies will be performed to confirm the strain persistence and safety in a larger number of individuals.

Oral iron supplementation leads to oxidative imbalance in anemic women: A prospective study

This study was aimed to assess the desirable and undesirable effects of iron (100mg/day as ferrous sulphate) and folic acid (500μg/day) supplementation in iron deficient anemic women. Iron and folic acid supplementations were given to 117 anemic women (mild=55, moderate=40, and severe=22) and 60 age matched placebo treated (100mg cane sugar) non-anemic controls for 100 days. Blood index values, oxidative stress parameters, antioxidant enzymes and vitamins were estimated as per standard protocols. Haemoglobin (Hb) levels along with antioxidant enzymes, namely catalase, superoxide dismutase (SOD), glutathione reductase (GSH-Rd), reduced glutathione (GSH) and total antioxidant capacity (TAC) were found significantly increased (P<0.01) in anemic women after treatment. However, the glutathione peroxidase (GSH-Px) and antioxidant vitamins A, C and E were found significantly decreased (P<0.01) in all treated groups. Lipid peroxide levels (LPO), protein carbonyl (PC), conjugated dienes (CD), lipid hydroperoxide (LOOH) and oxidized glutathione (GSSG) levels were found significantly increased (P<0.01) after oral iron supplementation groups. Moreover, undesirable side effects of iron supplementation were observed maximally in mild as compared with moderate and severe anemic groups, whereas nausea, vomiting, systemic reactions were negligible in all treated subjects. Study found recommended dose of iron effective for improving Hb, but at the cost of increased oxidative stress (mild>moderate>severe). It is suggested that blind iron supplementation should be avoided and shall be provided on need basis.

Evaluation of a Point‐of‐Care Blood Analyzer for Use in Determination of Select Hematological Indices in the Seminole Killifish

AbstractConventional methodologies for hematological analysis are gradually being replaced with new technologies. Point‐of‐care blood analyzers are both efficient and user friendly. As the use of such technologies becomes more pervasive in current literature, investigations into the accuracy and reliability of point‐of‐care analyzers for evaluating fish hematological indices are warranted. The purpose of this study was to evaluate a point‐of‐care blood analyzer (i‐STAT®) and chosen cartridge (E3+) against conventionally accepted instrumentation (CAI) for use in determination of hematocrit, sodium, potassium, and chloride values in Seminole killifish Fundulus seminolis. Whole blood, a whole‐blood heparin dilution, and undiluted plasma were analyzed in the point‐of‐care unit to determine hematological parameters of interest. The Bland–Altman method for assessing agreement between two methods of clinical measurement was used as well as calculations of correlation coefficients and two‐tailed paired Student's t‐tests. Generally, mean values obtained from CAI were higher than those from the i‐STAT using whole blood, heparin‐diluted blood, and plasma. Significant differences (P ≤ 0.05) were observed between all means analyzed by t‐tests. When compared with CAI results, none of the blood index values generated by the i‐STAT in these experiments could be considered reliable.