Use Of Various Antibiotics Research Articles

Fabrication of a novel Ag2S-ZnS/ZIF-8 hollow heterojunction by in-situ formation of Ag2S and ZnS on ZIF-8 with enhanced tetracycline degradation performance

Currently, antibiotics are extensively utilized in the pharmaceutical industry to assist humans and animals in combating various infectious diseases. The extensive use of various antibiotics enhances the quality of life. However, it can also have negative effects on the environment and human health, including water pollution and the rise of antibiotic-resistant microorganisms. To date, antibiotics have been detected in rivers, groundwater, and even drinking water. There is an urgent need to design and develop advanced materials for the remediation of antibiotics present in our environment. In this paper, we successfully synthesized a novel Ag2S-ZnS/ZIF-8 hollow heterojunction via facile sulphuration and solvothermal methods. This heterojunction was employed as a catalyst for the degradation of tetracycline (TC) in water under UV and visible light irradiation. The physicochemical properties of the as-synthesized catalysts were thoroughly analyzed using XRD, EDS, SEM, BET, XPS, TEM, ESR, UV–vis techniques. The results demonstrate that Ag2S-ZnS/ZIF-8 displays enhanced photocatalytic activity for TC degradation no matter under visible or UV light irradiation. After 10 min of UV light irradiation, its photocatalytic degradation efficiency was achieved 92.74 %. Additionally, Ag2S-ZnS/ZIF-8 was able to degrade 92.14 % of TC (visible light, with 35 min). The corresponding kinetic rate constant of Ag2S-ZnS/ZIF-8 is 0.145 min−1 (UV light) and 0.043 min−1 (visible light), respectively. Furthermore, Ag2S-ZnS/ZIF-8 also shown excellent stability for the degradation of TC in the presence of visible light. This is a result of its high specific surface area, superior photocurrent and efficient separation of photo-generated electrons-hole pairs. Apart from this, the photocatalytic degradation mechanism of TC over Ag2S-ZnS/ZIF-8 is discussed in detail.

Impacts of cefalexin on nitrite accumulation, antibiotic degradation, and microbial community structure in nitrification systems

The intensive use of various antibiotics for clinical and agricultural purposes has resulted in their widespread use in wastewater treatment plants. However, little research has been conducted on the effects of antibiotics on nitrite accumulation, antibiotic degradation pathways, or the microbial community structure in nitrification systems. In this study, a laboratory-scale sequencing batch reactor was used to treat wastewater containing cefalexin (CFX) at different doses (5, 10, 15, and 20 mg/L). The results showed that the nitrification performance was gradually inhibited with increasing CFX concentration. Ammonia-oxidizing bacteria (AOB) are more tolerant to CFX than nitrite-oxidizing bacteria (NOB). Under 15 mg/L of CFX, NOB were completely suppressed, whereas AOB were partially inhibited, as evidenced by an ammonium removal efficiency of 60 % and a 90 % of nitrite accumulation ratio. The partial nitritation was achieved. CFX can be degraded into 2-hydroxy-3phenylpyrazine and cyclohexane through bacterial co-metabolism, and CFX degradation gradually diminishes with decreasing nitrification performance. The abundance of Nitrospira gradually decreased with increasing CFX concentration. Ferruginibacter, Hydrogenophaga, Thauera, and Pseudoxanthomonas were detected at relative abundances of 13.2 %, 0.4 %, 0.9 %, and 1.3 %, respectively, indicating their potential roles in antibiotic degradation. These findings provide insight into the interactions between antibiotics and microbial communities, which are beneficial for a better understanding of antibiotic degradation in nitrification systems.

MICROFLORA OF COMBAT WOUNDS OF THE MALE PELVIC ORGANS AND DYSBACTERIOSIS OF THE URINARY SYSTEM

Introduction. Identification of microorganisms that colonize combat wounds and cause wound infection is of primary importance for the subsequent successful treatment of the patient. The resistance of microorganisms to antimicrobial drugs makes the efforts of modern medicine in the fight against infectious agents ineffective. The problem of infertility is closely related to combat injuries, their infection, stress, and neurotic disorders. Aim. Obtaining and summarizing data on microbial colonization of mine-blast wounds of pelvic organs and the microbiome of the genitourinary system of combatants. Materials and methods. 84 smears were taken from 56 wounds of 36 patients with injuries of the pelvic organs who were being treated. 73 patients with injuries were examined for the presence of mycoflora in the urogenital tract. Isolation of pure bacterial cultures was carried out by inoculating the studied material using meat-peptone agar, blood agar, chromogenic agars. For the diagnosis of urogenital or other infections by the PCR method, a scraping from the back wall of the urethra was taken from the patients. Results. Predominant microorganisms in positive cultures of smears were non-fermenting gram-negative rods, which in 28% of cultures belonged to the genus Acinetobacter, in 26% to the genus Pseudomonas. As for associated infections, 20% of them consisted of the genus Acinetobacter, 32% – Enterobacter, 4% – Klebsiella and 29% – Pseudomonas. Gram-positive cocci were isolated in 37% of positive smear cultures. The frequency of isolation of the genus Streptococcus in monoinfection was 2.5%, followed by the genus Clostridium – 2%, Bacillus – 3%, Enterococcus – 4% and Actynomycceas – 4%. In associated infections, the frequency of isolation of the genus Streptococcus was 4%, followed by the genus Clostridium – 2%, Bacillus – 4%, Enterococcus – 3% and Actynomycceas – 5%. When analyzing the microflora of the genitourinary system, it was found that the priority role belongs to the combined infection, when there are associations of specific pathogens such as Ureaplasma spp., Mycoplasma spp., Chlamidia spp., Neisseria gonorrhoeae, Trichomonas vaginalis, Streptococcus spp., Enterococcus faecalis, which is 80% of the entire microbiome. Conclusions. Acinetobacter baumanii and Klebsiella pneumoniae are the dominant microflora complicating the course of combat wounds during almost two years of Russia's full-scale war against Ukraine. Probably, the duration of hostilities, the large number of wounded, and the forced mass unsystematic use of various antibiotics lead to rapid changes in the spectrum of pathogens of combat wounds. Combat wounds and their infection, stress, and nervous disorders lead to an imbalance of microflora, in particular microflora of the genitourinary system, which can be one of the causes of infertility. Chlamydia and Ureaplasma are the most common microorganisms that colonize the urogenital tract of men injured as a result of hostilities.

Phytobiotics: Response to Aquaculture as Substitute of Antibiotics and other Chemical Additives

The use of phytobiotics in aquafarming is nowadays an interesting topic because of its eco-friendly nature for the growth and antimicrobial performance of aqua livestock. Currently, the use of various antibiotics in aquaculture are restricted due to concerns about the potential development of antibiotic-resistant pathogenic bacteria. Overuse of antibiotics and other chemicals (like different disinfectants, chemicals, and preservatives) increases the potentiality of residual antibiotics and other chemicals being present in fish, fish flesh as well as in fishery products. Various phytobiotics from plant extracts or direct plant or animal resources have changed the present traditional scenario of using an antibiotic component against livestock performance in aquaculture. The bioactive compounds (like alkaloids, tannins, saponins, glycosides, terpenoids, phenolics, flavonoids, essential oils, or steroids) present in the phytobiotics act as an immunity booster, growth promoter, bio-appetizer, reproductive stimulator, bio-preservatives, etc. So, phytobiotics becoming an effective additive in aquaculture as a substitute for antibiotics and other chemical components. This present article is designed to provide a comprehensive and insightful overview of the current state of knowledge about phytobiotics that supports the need for sustainable and environmental friendly practices in the context of aquaculture.

Cu-hemin multilayer nanosheets with integration of peroxidase and photothermal properties for efficient synergistic sterilization

The increasing drug resistance of bacteria due to the inappropriate use of various antibiotics has become an ongoing public health problem around the world. In this work, a novel Cu-hemin nanosheet with multilayer structure was synthesized by a simple green method. Integrating the peroxidase properties of hemin with the excellent photothermal conversion performance of IR780, the as-prepared Cu-hemin@IR780 can not only effectively catalyze hydrogen peroxide to produce reactive oxygen species (ROS), but also has a highly efficient and stable photothermal effect for sterilization. The antibacterial efficiency against MRSA can reach up to 98.96% by using chemodynamic therapy (CDT) and photothermal therapy (PTT) for synergistic sterilization.

Current status and development prospects of aquatic vaccines.

Diseases are a significant impediment to aquaculture's sustainable and healthy growth. The aquaculture industry is suffering significant financial losses as a result of the worsening water quality and increasing frequency of aquatic disease outbreaks caused by the expansion of aquaculture. Drug control, immunoprophylaxis, ecologically integrated control, etc. are the principal control strategies for fish infections. For a long time, the prevention and control of aquatic diseases have mainly relied on the use of various antibiotics and chemical drugs. However, long-term use of chemical inputs not only increases pathogenic bacteria resistance but also damages the fish and aquaculture environments, resulting in drug residues in aquatic products, severely impeding the development of the aquaculture industry. The development and use of aquatic vaccines are the safest and most effective ways to prevent aquatic animal diseases and preserve the health and sustainability of aquaculture. To give references for the development and implementation of aquatic vaccines, this study reviews the development history, types, inoculation techniques, mechanisms of action, development prospects, and challenges encountered with aquatic vaccines.

Genomic Characterization of Clinical Acinetobacter baumannii Isolates Obtained from COVID-19 Patients in Russia.

The coronavirus disease 2019 (COVID-19) pandemic has already affected all realms of public healthcare and, in particular, has led to increasing use of various antibiotics to treat possible bacterial coinfections even in cases for which such infections were not confirmed clinically. This could lead to an increase in the fraction and severity of multidrug-resistant bacterial isolates in healthcare facilities, especially in intensive care units (ICU). However, detailed epidemiological investigations, possibly including whole genome sequencing (WGS), are required to confirm the increase in antibiotic resistance and changes, if any, in the population and clonal structures of bacterial pathogens. In this study, we performed a comprehensive genomic and phenotypic characterization of selected multidrug-resistant A. baumannii isolates obtained from the patients of a dedicated COVID-19 ICU in Moscow, Russia. Hybrid short- and long-read sequencing allowed us to obtain complete profiles of genomic antimicrobial resistance and virulence determinants, as well as to reveal the plasmid structure. We demonstrated the genomic similarity in terms of cgMLST profiles of the isolates studied with a clone previously identified in the same facility. We believe that the data provided will contribute to better understanding the changes imposed by the COVID-19 pandemic on the population structure and the antimicrobial resistance of bacterial pathogens in healthcare facilities.

Innate Lymphoid Cells and Natural Killer Cells in Bacterial Infections: Function, Dysregulation, and Therapeutic Targets.

Infectious diseases represent one of the largest medical challenges worldwide. Bacterial infections, in particular, remain a pertinent health challenge and burden. Moreover, such infections increase over time due to the continuous use of various antibiotics without medical need, thus leading to several side effects and bacterial resistance. Our innate immune system represents our first line of defense against any foreign pathogens. This system comprises the innate lymphoid cells (ILCs), including natural killer (NK) cells that are critical players in establishing homeostasis and immunity against infections. ILCs are a group of functionally heterogenous but potent innate immune effector cells that constitute tissue-resident sentinels against intracellular and extracellular bacterial infections. Being a nascent subset of innate lymphocytes, their role in bacterial infections is not clearly understood. Furthermore, these pathogens have developed methods to evade the host immune system, and hence permit infection spread and tissue damage. In this review, we highlight the role of the different ILC populations in various bacterial infections and the possible ways of immune evasion. Additionally, potential immunotherapies to manipulate ILC responses will be briefly discussed.

The Influence of Diet and Probiotics on the Response of Solid Tumours to Immunotherapy: Present and Future Perspectives

Currently, various immunotherapeutic treatments are revolutionizing therapies that treat solid neoplasms. For these treatments, within immunotherapy, immune checkpoint inhibitors (ICIs) are the most widely used drugs. Diverse studies have shown the influence of diet and probiotics on the response to ICIs and consequently on the survival rates associated with different neoplasms. The use of various antibiotics, probiotics, and prebiotics has been associated with changes in the gut microbiota, and this, in turn, with resistance to immunotherapy. Together with the above, a lower intake of red meat and greater consumption of a Mediterranean, vegetarian, or vegan diet have led to a new way of understanding the mechanisms of resistance to ICIs. Omega-3 and polyphenol supplements are also powerful regulators of the microbiome whose influence on the immune system. Therefore, this review covers the influence of diet and probiotics on the response to immunotherapy in patients who have solid tumours.

Alternative Strategies in the Treatment of Clostridioides difficile Infection

Clostridioides difficile (C. diff.) is a leading cause of nosocomial infections worldwide and is a major challenge to public health. Widespread use of antibiotic agents have caused increasing incidence rates of C. diff. infections and the emergence of antibiotic-resistant strains of the potentially-deadly bacteria. The current treatment guidelines include the use of various antibiotics, which further contributes to the problem of antibiotic resistance. There is an urgent need for novel treatment methods in order to halt the emergence of even more antibiotic-resistant bacteria. This review discusses the pathogenesis of C. diff. infections, current treatment strategies, and possible alternative treatment strategies based on breakthrough scientific research.

Screening of bacterial kidney disease in the North-West region of the Russian Federation

Our country has all potential for development of an aquaculture as one of sectors of agriculture, due to a huge number of fresh water areas and the extended line of the seas. Bacterial kidney disease, a chronic disease in the Salmonidae family, causes significant damage to aquaculture development. Modern proposals for the use of various antibiotics and chemotherapeutic drugs do not bring a significant effect to control the spread of this disease, and due to the peculiarity of the pathogenesis of the disease, the development of an effective vaccine against bacterial kidney disease is currently difficult, therefore the most effective method of controlling and preventing the disease is early diagnosis of the disease. The article discusses the results of exploratory studies of bacterial kidney disease in fish-breeding enterprises of the North-West region of the Russian Federation for the period from March 2019 to March 2020.

Detection and analysis of antibiotic resistance variability among Staphylococcus aureus isolates from animal and human sources

In consideration of the importance of Staphylococcus aureus regarding its contribution to antibiotic resistance, the present study was designed to find variability among S. aureus isolates in relation to their multidrug resistance patterns. A total of 157 species-specific 23S rRNA based confirmed S. aureus isolates from various clinical and non-clinical animal sources (cattle, buffalo, goat, sheep, dog, camel, pig and horse), human and pieces of meat from butcher shops were included in the present study. Overall more than 95% isolates were recorded resistant to ampicillin and penicillin-G, while approximately 100% isolates were sensitive to chloramphenicol, meropenem and nitrofurantoin. The isolates from different sources showed highly significant (P≤0.01) variation in their resistance patterns for 39 antibiotics, significant variation (p≤0.05) for levofloxacin and nitrofurantoin, and no significant variation (P>0.05) for clindamycin. In Bonferroni correction, human isolates were significantly variable with a p˂0.0001 probability level of variance in relation to other pieces of meat and animal origin sources for most of the antibiotics. Human isolates had the highest (0.40) MAR index. A highly significant difference was observed in the antibiogram pattern between different sources of S. aureus, which may indicate the pattern and frequency of use of various antibiotics in humans and animals.

Osteomyelitis in the feline pelvic limb secondary to bacterial infection: Case report

Osteomyelitis is defined as bone inflammation caused by an infectious agent that may be bacteria or fungi, including associated structures such as soft tissues, periosteum and endosteum. The causes for the appearance of osteomyelitis are diverse, such as exposed fractures, traumatism and extensive bone exposure. The main clinical signs found are claudication in the affected limb, pain on palpation of the region, edema and presence of purulent discharge at the affected site. The diagnosis involves radiographic, hematological and antibiogram examinations. The treatment is long and requires persistence of both the tutor and the professional responsible. The best form of prevention is the attention and care in any injury that the animal may present in order to avoid outbreaks of infection. The objective of this study was to report a case of a 3-years-old feline, with symptomatology of pain, claudication and presence of mucopurulent secretion in the pelvic limb, from the city of Manaus, at the Veterinary Medical Center of Manaus, approaching clinical history, diagnosis and treatment. Through physical and radiographic examinations and antibiogram, it was possible to diagnose osteomyelitis. Treatment consisted of scraping, removal bone fragments and use of various antibiotics, with lincomycin showing the best results. The animal presented a satisfactory recovery. It was concluded that, through clinical signs, the correct approach and complementary tests such as bacterial culture and antibiogram is necessary to treat the infection.

On the innate immune response to intracellular bacterial infections

Various bacteria are able to infect human organisms to induce less or more dangerous diseases. In response to them, the human immune system is usually activated. In addition, medical therapies to the patients can be applied. One of the most popular approaches is the use of various antibiotics. However, some bacteria are able to develop resistance against the applied antibiotics. Various approaches to study these phenomena are proposed in the literature, among them tools for big data analysis, machine learning, mathematical modeling, etc. In our paper, we consider a mathematical model describing the interactions between susceptible uninfected cells, infected cells, immune cells and bacteria. We study the properties of the model. We propose numerical scheme for obtaining the approximate solutions to the model system for various values of parameters and initial conditions.

Pathogenic gut microbiota associated with striped catfish, Pangasianodon hypophthalmus cultured in Bangladesh and their antibiotic sensitivity pattern

To identify the gut bacteria of Pangasianodon hypophthalmus (striped catfish; Pangas) using classical and molecular approach was carried out. Total bacterial count (TBC) in the gut of pangas from farm and market samples were found 5.07 ± 1.70 × 106 and 1.40 ± 0.47 × 106 cfu/g, respectively. The gut microbiota of pangas was dominated by members of the Gram-negative genera. Only three isolates (MyF1/1, MyF1/4 and GaW1/2) were found to be Gram-positive among the 16 representative isolates. Using 16S rRNA gene sequencing; Bacillus, Macrococcus, Citrobacter, Aeromonas, Proteus, Klebsiella, Enterobacter, Escherichia and Edwardsiella were found to be associated with the gut of this fish. Among them, Aeromonas was the most dominant genus (5 out of 16). Antibiotic sensitivity pattern reflected that all the isolates were sensitive to gentamycin. Multiple antibiotics resistant isolates were also identified of which MyF3/13 (identified as Citrobacter amalonaticus) was found resistant against seven tested antibiotics. The presence of pathogenic bacteria in fish gut revealed the improper handling practices in fish market and unhygienic condition in the culture sites which might be a reason of fish-borne disease outbreaks. On the other hand, widespread use of various antibiotics in aquaculture without proper awareness may lead to resistance to multiple antibiotics.
 Dhaka Univ. J. Biol. Sci. 29(1): 61-73, 2020 (January)

Study on use of antibiotics for prophylaxis of surgical site infection and compare with standard guidelines

Background: Surgical site infection (SSI) is an infection that occurs at or near a surgical incision within 30 days of surgery or within 1 year if an implant is left in place. In low- and middle-income countries, SSI incidence may be approximately up to 4 times higher than in high-income countries. Proper choice of antibiotic is very important to prevent SSI. Aims and Objectives: This study was conducted with objectives to know the incidence of SSI, to study the use of various antibiotics for the prevention of SSI, and to compare the data with standard guidelines. Materials and Methods: An observational, prospective study was conducted after getting ethical clearance. All the patients, admitted in post-operative surgical wards, were enrolled in the study over the period of 2 months after getting informed consent. Data were collected from pre-formed Google questionnaire filled by the patients and indoor patients’ file evaluated further. Results: Out of 198 enrolled patients, 192 patients were given antibiotics as single drug or in combination of two or three. Overall incidence of SSI was 7.57%. The highest incidence rate of SSI was reported in general surgery department, 12.69%. Cefazolin was prescribed as surgical antibiotic prophylaxis (SAP) in all the cases of general surgery. Out of 198, a total of 128 (64.64%) patients were given a single drug as prophylactic antibiotic. In total 124 patients, the duration of antibiotic prophylaxis was

Frequency and etiology of tubulo-interstitial nephritis in an adult renal biopsies in a tertiary renal care hospital: A single-center study.

Tubulo-interstitial nephritis (TIN) is an important cause of acute renal failure which may progresses to chronic kidney disease (CKD). TIN is often under diagnosed with there are no specific signs and symptoms. As this entity has paramount importance, so we evaluated the frequency and etiological of TIN both acute TIN (ATIN) and chronic tububulo-interstitial nephritis (CTIN) in renal biopsies. This is a retrospective observational, descriptive study carried out in the Department of Nephrology at The Kidney Centre Post Graduate Training Institute from 2004 to 2016. A total of 1560 adult renal biopsies were done during this period with 125 biopsies of TIN, of which 70 (56%) cases were ATIN and 55 (44%), were CTIN. Thirty-eight (30%) patients had a history of taking proton-pump inhibitors, use of various antibiotics in 21 (16%) cases, and 11 (8%) patients had a history of taking Hakeemi (traditional healer using herbs and sometimes trace amounts of heavy metals) medications. The incidence of TIN is higher than suspected and can be caused by variety of etiological agents. Therefore, clinical awareness will help in the diagnosis and early identification of the disease.

Antifungal and Antibacterial Properties of Graphene-based Nanomaterials: A Mini-review

In recent years, the availability and use of various antibiotics and antimicrobial agents have resulted in increase of drug resistant pathogens. Therefore, scientist’s attention has been diverted to find a suitable replacement for antimicrobial treatment. Graphene (G), as a two-dimensional (2D) carbon-based nanomaterials (CBNs) has a unique physicochemical properties including thermal, optical and electrically conductive activities along with high surface-to-volume ratio and mechanical strength. Also, they have gained significant attention in biomedical application, such as regenerative medicine and drug delivery carriers. With the emergent of nanomaterials over the past decades, there has been a growing interest in using GBNs to develop new antifungal and antimicrobial nanomaterials due to their diverse antifungal and bactericidal mechanisms and relatively low cytotoxicity towards mammalian cells. Numerous reviews on GBNs are available with different perspective. In this review, we summarized the latest progresses towards an understanding of the antifungal and antibacterial properties of GBNs for developing a new antifungal and antibacterial materials.

The Anti-Helicobacter pylori Effects of Lactobacillus acidophilus, L. plantarum, and L. rhamnosus in Stomach Tissue of C57BL/6 Mice

Background and Aim: Helicobacter pylori is one of the most common pathogenic bacteria in the human gut, and is also one of the most important factors that cause digestive disorders such as chronic inflammation, gastric ulcers, and even gastric cancer. Since the use of various antibiotics to treat H. pylori infection is associated with the development of resistance in this bacterium, the aim of this study was to determine the anti-H. pylori effects of Lactobacillus acidophilus, L. plantarum, and L. rhamnosus in the stomach tissue of C57BL/6 mice. Materials and Methods: In this experimental study, 70 mice in ten groups were evaluated from July to September 2017 in the microbiology laboratory of the School of Medicine, Alborz University of Medical Sciences, Karaj, Iran. After induction of H. pylori infection in mice with the standard strain of H. pylori (ATCC 43504), the infected mice were treated with drug and Lactobacillus species in different groups. Then, the anti-H. pylori effects of lactobacilli were evaluated by stool antigen test and tissue staining. Results: Based on ELISA results and histological findings, a reduction of inflammation was observed. The group which was only exposed to L. rhamnosus and the one which was exposed to all three strains of Lactobacillus showed the highest antimicrobial effect on H. pylori. Conclusion: According to the results of this study, probiotic bacteria including L. acidophilus, L. plantarum, and L. rhamnosus could be useful in the reduction of H. pylori infection in the mouse model.

Relapse multifocal osteomyelitis secondary to septicemia: A case study

Osteomyelitis is an inexorable and debilitating infection of bones. Mostly, it is a stubborn recurrent infection and follows a long aggressive medical and surgical treatment. The present patient (54 year old female) is a case of chronic osteomyelitis with open trauma following septicemia as a result of accident. The patient got an open fracture of proximal right tibia, and surgical intervention along with pharmacological treatment was performed. A couple of days later, it was noted that there was swelling of the patient right leg, and pus discharge was evident. This infection was due to Enterobacter species as identified by laboratory results. Surgery was repeated for a complete debridement of wound. The case is of unusual interest because of its relentless nature, as even after, more than 38 months of treatment, the patient still suffers from the same discomfort of osteomyelitis and pus discharge is frequently being observed. Prolonged use of various antibiotics (ciprofloxacin, penicillin, fusidic acid, and cloxacillin) was found to control pus discharge to some extent controlled by the usage of pus discharge.