Use Of PRF Research Articles

Effect of Ethyl-Cyanoacrylate and Platelet-Rich Fibrin on Fresh Sockets of Rabbits Subjected to Anticoagulant Therapy.

Objectives: There are no studies related to the use of PRF associated with cyanoacrylates in fresh post-extraction sockets. Thus, the aim of this study was to assess the effect of ethyl-cyanoacrylate combined with PRF in fresh sockets of rabbits subjected to anticoagulant therapy. Methods: Twelve adults rabbits were selected and premedicated with heparin 1 week before surgery to induce and simulate anticoagulant therapy. Upper and lower first premolars on the right side were extracted and then were divided into four groups of three animals each, with the groups distributed according to the type of intervention in the sockets (n = 6): (1) clot and suture (control); (2) PRF and suture; (3) clot and ethyl-cyanoacrylate; (4) PRF and ethyl-cyanoacrylate. At 12 weeks, the animals were sacrificed and the sockets were analyzed histologically and quantitatively. Total bone area, inflammation infiltrate, and adhesive remnants were assessed. Results: No remnants of adhesive were found in the samples. Groups 1 and 2 showed the highest bone area (G1 = 37.87% ± 17.86; G2 = 30.31 ± 9.36) with significant differences to those treated with ethyl-cyanoacrylate adhesive (G3 = 26.6% ± 11.82; G4 = 24.29% ± 6.25). Conclusions: The groups that used ethyl-cyanoacrylate as a closure method in sockets exhibited less bone area than the groups that used sutures. Both groups that used PRF as therapy did not show a significant improvement in bone healing at 12 weeks compared with the clot groups.

FIBRIN-RICH PLASMA (PRF) EFFECT OF PLATELET COUNT ON BONE TISSUE REGENERATION

The use of autologous platelet concentrates, mainly Fibrin-Rich Plasma (PRF), has been increasingly used in dentistry as a bioactive material that presents itself as a treatment alternative for various clinical situations, such as example, periodontal defects, furcation lesions, osteonecrosis of the jaw, oral communication, maxillary sinus lifting, implant surgeries, regenerative surgeries of both soft and hard tissues and, in addition, it contributes positively to better tissue healing. Therefore, the present work consists of a systematic review of the literature seeking to analyze the evidence and effectiveness of the use of PRF in regenerative treatments. The research was carried out using the online database: PUBMED, choosing publications made in the last 5 years (2019-2024). A total of 697 articles were found, however, after applying the inclusion criteria, bias analysis and selection, 10 articles were selected. The results found in the present work allow us to affirm that the use of PRF in bone regeneration has positive and satisfactory effects.

Blood Collection for Autologous Blood-Derived Product Preparation: Technique and Application

Background: In periodontics, interest in use of autologous blood-derived products (ABPs) has increased due to demonstrated safety, enhanced initial healing, and for some applications, superior clinical outcomes. Periodontists commonly place peripheral intravenous catheters for moderate sedation, thus encountering opportunities to utilize ABPs routinely. The purpose of this report is to describe a safe blood collection technique for ABP preparation and define typical blood volume requirements for periodontal procedures. Methods: Five cases requiring various amounts of liquid and membrane-formed platelet-rich fibrin are presented. Case 1 involves treatment of an infrabony periodontal defect. Case 2 illustrates alveolar ridge preservation at a maxillary right second premolar site. Case 3 demonstrates repair of a defect related to nasopalatine duct cyst removal. Cases 4 and 5 illustrate the use of PRF in sinus elevation and root coverage, respectively. Results: Use of PRF in the presented cases added minimal procedural time and expense. Blood samples varied from 20 to 60 ml in volume. There were no complications related to blood collection or use of PRF. Each patient reported minimal discomfort limited to the first few postoperative days. Favorable early healing was observed in each case. Conclusions: The blood collection method described in this report, which is consistent with published standards of practice, necessitates few additional steps and supplies for practitioners already placing peripheral intravenous catheters. Blood volumes necessary for typical procedures in periodontics are safe and well below maximum attainable samples.

Evaluation of Modified Shell Technique in 3D Ridge Reconstruction: A Clinical and Radiographic Study

Introduction: As a result of tooth loss, alveolar ridge resorption sacrifices bone volume including bone width. In order to replace the lost dentition with implants functionally and esthetically, bone augmentation procedures are carried out. Materials and Methods: Fifteen patients were selected to treat mandibular alveolar ridge resorption with an autogenous block graft harvested using piezoelectric surgical tips and split into two shells using a diamond disk then fixated on the decorticated defective ridge. Particulate xenograft bovine bone was then inserted between the fixated shell and ridge, the second shell was used to roof the bone particles. Results: The modified shell technique could be an alternative to other ridge augmentation techniques. This study shows promising clinical and radiographic results that carry the advantages of being: safe and precise cutting using Piezoelectric tips, the statistical data showed a significant difference in 3D bone volume where no unfavorable complication was detected. Conclusion: The modified shell technique showed a reliable technique in cases of 3D ridge reconstruction since it holds the advantages of autogenous bone properties and the rigidity of the bone shells that maintain the space necessary for augmentation. The use of PRF can accelerate wound healing and minimize the risk of flap dehiscence as well as it can speed up bone formation.

To evaluate and compare the synergetic effect of Cranberry extract with PRF versus the use of PRF alone in the treatment of Chronic Periodontitis: A Clinical and a Radiological study

To evaluate and compare the synergetic effect of Cranberry extract with PRF versus the use of PRF alone in the treatment of Chronic Periodontitis: A Clinical and a Radiological study

Effectiveness of platelet rich fibrin alone or in combination with bone grafts in the treatment of infrabony defects: Systematic review and metanalysis

This systematic review aimed to investigate the effectiveness of PRF alone or in combination with bone grafts in enhancement of tissue/bone formation in infrabony defects. A search was performed in electronic databases (i.e. Pubmed/MEDLINE, EMBASE, Cochrane, Clinical trials.org) in order to identify Randomized Control Trials (RCTs). The last electronic search was run on 24 August 2021 From the search, 21 articles fulfilled the inclusion criteria and were quantitatively analyzed. In total, there were 13 articles with low risk of bias, 4 with some concerns and 4 with high risk. The meta-analysis showed that there was a statistically significant benefit from the use of PRF alone or in combination with bone graft after 6 months follow up in clinical attachment level (CAL), periodontal probing depth (PPD) and bone fill (BF). However, there was not a significant difference in CAL and PPD after 9 months In summary the use of PRF was effective in the treatment of periodontal infrabony defects especially in CAL and BF after 6 months follow up. From the interpretation of the results, we indicate that the combination of PRF with grafting materials may accelerate the bone healing process in the treatment of intrabony defects.

Can Platelet Rich Fibrin enhance the role of alloplastic bone graft in socket preservation: A Randomized Clinical Trial

Socket preservation aims at limiting the resorptive events that occurs as a consequence of tooth extraction. Besides the use of autologous and commercially available graft materials, there has been an increasing shift towards the utilization of biological substitutes like Platelet Rich Fibrin for socket preservation. : A randomized clinical trial was conducted in fourteen extraction sites, out of which seven were filled with alloplastic bone graft material alone (control group) and rest were filled with alloplastic bone graft material along with PRF (test group). The sites were assessed clinically and radiographically at baseline and six months post treatment. The mean +/- SD was calculated for alveolar ridge width and height. Although alveolar ridge width and height reduced significantly in the span of six months in both test and control groups, there was no statistically significant intergroup difference in both groups at six months post treatment in these parameters. Wound healing was comparatively better in the test group compared to the control group initially, but at the end of the study period both groups attained same level of healing. Even though alveolar socket preservation has a clinically significant effect on preserving the residual ridge dimensions, use of PRF as an adjunct to alloplastic bone graft in socket preservation procedure has no additional benefit over alloplastic bone graft material alone.

Injectable Platelet-Rich Fibrin as a Drug Carrier Increases the Antibacterial Susceptibility of Antibiotic-Clindamycin Phosphate.

The aim of this study was to investigate the change in clindamycin phosphate antibacterial properties against Gram-positive bacteria using the platelet-rich fibrin as a carrier matrix, and evaluate the changes in the antibiotic within the matrix. The antibacterial properties of CLP and its combination with PRF were tested in a microdilution test against reference cultures and clinical isolates of Staphylococcus aureus (S. aureus) or Staphylococcus epidermidis (S. epidermidis). Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) analysis was done to evaluate the changes in the PRF_CLP matrix. Release kinetics of CLP was defined with ultra-performance liquid chromatography (UPLC). According to FTIR data, the use of PRF as a carrier for CLP ensured the structural changes in the CLP toward a more active form of clindamycin. A significant decrease in minimal bactericidal concentration values (from 1000 µg/mL to 62 µg/mL) against reference cultures and clinical isolates of S. aureus and S. epidermidis was observed for the CLP and PRF samples if compared to pure CLP solution. In vitro cell viability tests showed that PRF and PRF with CLP have higher cell viability than 70% after 24 h and 48 h time points. This article indicates that CLP in combination with PRF showed higher antibacterial activity against S. aureus and S. epidermidis compared to pure CLP solution. This modified PRF could be used as a novel method to increase drug delivery and efficacy, and to reduce the risk of postoperative infection.

Sinus Lift Associated with Leucocyte-Platelet-Rich Fibrin (Second Generation) for Bone Gain: A Systematic Review.

The purpose of this systematic review was to analyze sinus lifting procedures and to compare the efficiency of this treatment associated with the second generation of platelet-rich fibrin related to its effects on bone gain and to clarify the regenerative efficacy in sinus lift procedure, whether alone or as a coadjutant to other bone graft materials. The PICOT question was, “In clinical studies with patients needing a maxillary sinus lift (P), does the use of PRF either alone (I) or in conjunction with other biomaterials (C) improve the clinical outcome associated with bone gain and density (O), with at least three months of follow-up (T)?” An electronic search was conducted in the MEDLINE (PubMed), Science Direct, and Scopus databases through a search strategy. A total of 443 articles were obtained from the electronic database search. Sixteen articles met all criteria and were included in this review. Within the limitation of this study and interpreting the results carefully, it was suggested that a higher risk for implant failure after a sinus elevation might be seen in patients with residual bone ≤4 mm, and PRF application was effective, suggesting reducing the time needed for new bone formation.

What recent evidence exists to support the use of platelet‐rich fibrin in clinical dentistry? A systematic literature review

AbstractBackgroundPlatelet‐rich fibrin (PRF) is a second‐generation platelet concentrate and biomaterial with multiple applications in clinical dentistry. Recent developments in the production of PRF have resulted in several divergent protocols that could be seen to affect the reproducibility and generalisability of the available literature. Additionally, imprecise reporting on PRF production is a further confounding factor with respect to previous trial data.AimsThe primary aim of this review was to assess the recent clinical trial data relating to the use of PRF, irrespective of centrifugal protocol, in clinical dentistry.MethodsA systematic literature review was carried out in PubMed and the Cochrane Library databases to select English language, human clinical trials involving the use of PRF published between July 2018 and July 2021.ResultsThe 50 included studies demonstrated an overall clinical benefit for the use of PRF in the fields of oral medicine, oral surgery and periodontology, with unclear benefits in the field of endodontology, and no clear benefit in the field of orthodontics. Only 16% of the included studies met published standards for reporting PRF production protocols, and meeting such standards should be an essential requirement for future publications.ConclusionsThere was little direct evidence to support one particular PRF protocol over another and this remains an area of controversy. Future research should focus on precise reporting of PRF protocols, and in areas where PRF is of known benefit, the use of different production methods should be compared to assess what, if any, clinical significance is attached to particular centrifugation protocols and tubes.

The Assessment of the Usefulness of Platelet-Rich Fibrin in the Healing Process Bone Resorption

The main subject of this research was the use of PRF in dental surgery aimed at preventing changes in alveolar height and width after tooth extraction. Due to the large growth factor content, it seems to be particularly useful in bone loss management starting from the simplest loss occurring after tooth extraction through loss resulting from tooth resection ending with loss caused by large bone cysts. The study was performed on 50 patients. The extraction of two maxillary or mandibular homonymous teeth was carried out in each patient, where PRF was placed in one alveolus while the other alveolus was left empty. Then, the alveoli were surgically managed with a split flap technique. On the extraction day, after 10 days, and after 6 months, the alveolar process was measured, soft tissues healing was assessed, and imaging examinations were analyzed. It was proved that the healing of soft tissues in the PRF group was better. In the PRF group after 6 months from surgery, the newly formed bone had higher grayscale values in volumetric tomography (CBCT). Moreover, the reduced atrophy of the alveolar process at the site of the extracted tooth was proved in this study.

Non-Interventional Prospective Observational Study of Platelet Rich Fibrin as a Therapy Adjunctive in Patients with Medication-Related Osteonecrosis of the Jaw

Background: Medication-related osteonecrosis (MRONJ) of the jaw is a severe and feared side effect of antiresorptive therapy in the oncological setting. With growing evidence that impaired angiogenesis may represent a key factor in pathogenesis, the aim of this study was to evaluate an autologous platelet concentrate as a possible additive in surgical therapy to optimize vascularization and, subsequently, resolution rates. Material and Methods: A non-interventional, prospective, multicenter study was conducted, and all patients with stage I-III MRONJ, undergoing antiresorptive therapy for an oncological indication, were included. The necrosis was treated surgically without (study arm A) or with (arm B) the addition of an autologous platelet concentrate (platelet-rich fibrin, PRF). Results: After 5, 14, and 42 days postoperative, wound healing (primary outcome: mucosal integrity) as well as downstaging, pain perception, and oral health-related quality of life (secondary outcome) were assessed via clinical evaluation. Among the 52 patients included, primarily with MRONJ stage I and II, the use of PRF as an additive in surgical therapy did not display a significant advantage for wound healing (p = 0.302), downstaging (p = 0.9), pain reduction (p = 0.169), or quality of life (p = 0.9). Summary: In conclusion, PRF as an adjunct did not significantly optimize wound healing. Further, no significant changes in terms of downstaging, pain sensation, and oral health-related quality of life were found.

Clinical Application of Platelet-Rich Fibrin in Pediatric Dentistry

AbstractPlatelet-rich fibrin (PRF) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time and that can serve as a resorbable membrane. It can be obtained from blood with the help of a simple process. PRF is basically a concentrate of growth factors that promote wound healing and regeneration, which is used in various disciplines of dentistry to repair various lesions and regenerate dental and oral tissues. Although the use of PRF is well-documented, its use in pediatric dentistry remains unexplored, due to its restrictions and on account of the fact that it is a blood-derived product. PRF has been used globally to enhance tissue healing. This article provides an insight into the use of PRF in pediatric dentistry, its benefits, limitations, and recommendations.

TREATMENT OUTCOME OF DRY SOCKET FOLLOWING IMMEDIATE IMPLANT PLACEMENT USING PLATELET RICH FIBRIN: A PROSPECTIVE MULTI-CENTRE CLINICAL STUDY

Abstract: Objectives: Dry socket is a common post-extraction complication. It is possible to occur following immediate implant placement. This study was designed to assess the efficacy of PRF for the management of established dry socket following immediate implant placement. Materials and Methods: Twenty patients with established dry sockets following immediate implant placement were included. Patients were divided randomly, patients of group I were treated by socket curettage and irrigation, while in group II PRF was inserted in the space around the implant and filled the socket. Pain and granulation tissue formation score has been used to assess both groups. Results: The pain score was 6 to 10 on the day zero, 3 to 6 on the day four and 1 to 3 on the day seven in group I, while in group II the pain score was 6 to 10 on the day zero, 0 to 3 on the day four and 0 to 1 on the day seven (p 0.001). On group II the formations of granulation tissue appeared earlier than group I. Four implants failed, three implants in group I and one implant in group II. Conclusion: This study suggests that the use of PRF for the management of dry socket following immediate implant placement relieves pain, accelerates the process of wound healing and increases implant success rate.

Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

The results of a histomorphological study of bone regenerates in rabbits after osteosubstitution with platelet-rich fibrin and its combination with hydroxyapatite granules with β-tricalcium phosphate in spongy and compact bone tissue at different periods of reparative osteogenesis are presented. Three groups of rabbits were formed. In the control group, the defects were left to heal under the blood clot. Bone lesions in the first experimental group were filled with platelet-rich fibrin, in the second – with a combination of platelet-rich fibrin and hydroxyapatite with β-tricalcium phosphate. All animals were kept in the same conditions of feeding and housing, had unlimited access to water. During the study, the rabbits were monitored clinically. The animals were taken out of the experiment on the 14th, 21st and 42nd days, samples of bone tissue were taken, they were fixed, decalcified, dehydrated in alcohols of increasing concentration and embedded in paraffin. In the presented study, it is most likely that the newly formed bone tissue is formed precisely due to osteoinduction in the experimental groups. When using granules of hydroxyapatite and β-tricalcium phosphate with platelet-rich fibrin, bone regenerate is between the composite granules and is not associated with contact with the maternal bone. Its cells appear in different places of the defect. In the spongy bone tissue on the 21st day, regeneration proceeded more fully and faster in the second experimental group, as evidenced by the significant density of cells of the osteoblastic row, the thickness of the bone trabeculae and their volume, filling the site of the defect. On the 42nd day, in the second experimental group, when using a combination of autobiomaterial and hydroxyapatite granules with β-tricalcium phosphate, the regenerate contained a significantly larger number of osteogenic cells in the thickness of the trabeculae, which indicated a more intensive course of reparative osteogenesis in comparison with the first experimental and control groups. In the compact bone on day 21, regeneration was more complete and faster in the second experimental group. On the 42nd day, according to the degree of maturity of the bone regenerate, calcium-phosphate ceramics in combination with platelet-rich fibrin optimizes reparative osteogenesis most clearly. According to the degree of intensity of the osteoregeneration process, the groups can be placed in the following sequence: control ˂PRF˂PRF+HA/β-TCP–700. According to histomorphological characteristics, the combination of hydroxyapatite with β-tricalcium phosphate and platelet-rich fibrin gives greater osteoinduction to the composite material, which is confirmed by the high cell density, namely of osteoblasts and osteocytes. The use of PRF in combination with other materials may become promising for the correction of reparative osteogenesis in conditions of limited or reduced regenerative potential of bone tissue.

Procedures for production, storage and utilization of platelet-rich plasma and correlated products in Brazil: a cross-sectional study

Introdução: O plasma rico em plaquetas (PRP) é uma tecnologia com potencial aplicação em diversas áreas da medicina e odontologia. A alta variabilidade no processo de produção desta tecnologia e de tecnologias relacionadas desafiam a regulamentação de sua produção, do controle de qualidade e do uso. Objetivo: Avaliar práticas de produção, armazenamento e utilização do PRP e produtos relacionados no Brasil, permitindo a identificação de variabilidade operacional ao longo destes processos. Método: Estudo transversal com coleta de dados realizadas por meio de um questionário eletrônico, autoaplicado e com preservação de anonimato. As perguntas foram definidas a partir de revisão de literatura e por consulta a especialista da área. O questionário foi encaminhado a pesquisadores e a profissionais de várias áreas atualmente envolvidas na produção do PRP. Esses profissionais foram identificados por diferentes estratégias, tais como participação prévia em eventos relacionados e publicações na área. Resultados: Foram obtidas 64 respostas, sendo 36 respostas completas (56,3%) e 28 respostas parciais (43,7%). A taxa de respondentes não pôde ser calculada, considerando-se as formas de divulgação do questionário. Para a produção de PRP, as respostas indicaram predomínio de protocolos com centrifugação dupla e utilização de métodos variados para ativação plaquetária. A maioria dos respondentes referiu utilizar o PRP nas áreas de ortopedia, fisiatria e medicina esportiva. Para a produção do plasma rico em fibrina (PRF), houve predomínio de respostas indicando utilização de protocolo de centrifugação única, sem utilização de métodos de ativação plaquetária. A maioria dos respondentes indicou utilizar o PRF em áreas da odontologia. No geral, observou-se grande variabilidade nos procedimentos de obtenção e do perfil de utilização entre os principais tipos de concentrados de plaquetas. Conclusões: Os achados corroboram a necessidade do desenvolvimento de medidas regulatórias com foco nos pontos críticos identificados, visando promover a segurança durante a produção e utilização dos concentrados de plaqueta.

Platelet Rich Fibrin in the Treatment of Localised Gingival Recession: A Case Report

Root recession presents a matter of aesthetic concern in every dental practice. Also it may illicit sensitivity and is more prone to caries than the covered root surfaces. Platelet- rich fibrin is a second generation platelet concentrate. It is basically autologous leukocyte and platelet-rich fibrin biomaterial. The use of PRF in combination with coronally advanced flap in the treatment of gingival recession has shown promising results and is gaining immense popularity among the dental practitioners. This case reports the use of PRF membrane in root coverage on the labial surface of maxillary canine. Keywords: Coronally advanced flap, platelet rich fibrin, gingival recession, growth factors.

Efficacy of platelet rich fibrin in osseous regeneration after periapical surgeries

Efficacy of platelet rich fibrin in osseous regeneration after periapical surgeries - IJASHNB- Print ISSN No: - 2581-5210 Online ISSN No:- 2581-5229 Article DOI No:- 10.18231/j.ijashnb.2021.006, IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain-IP Indian J Anat Surg Head Neck Brain

The effects of platelet-rich fibrin on post-surgical complications following removal of impacted wisdom teeth: A pilot study

ObjectivesPlatelet-rich fibrin (PRF) is a fibrin matrix composed of cells, cytokines, and growth factors, which may be liberated from blood at specific times and act as a resorbable membrane. Many studies have demonstrated the benefits of PRF for bone healing in oral and maxillofacial implant surgery. Our study assesses the impact of PRF on postoperative complications following the extraction of impacted wisdom teeth. MethodsTwenty patients were recruited in this blind controlled randomised clinical trial, i.e. ten patients in the study group and ten patients in the control group. The patients were aged between 18 and 40 years. Surgical removal of impacted teeth #38 and #48 was performed in all the patients. The study group underwent dentoalveolar surgery with the use of PRF, while the control group underwent surgery without PRF. ResultsAll patients in the study group reported significantly less postoperative pain (p = 0.02) and excellent soft tissue healing during the follow-up period (p = 0.021). In contrast, only 80% of patients in the control group exhibited sufficient soft tissue healing. The difference in postoperative swelling between the two groups was not statistically significant. Additionally, 69% of patients in the study group reported comfortable night sleep during the first 24 h after the operation compared to 31% in the control group. This difference was statistically significant (p = 0.02). ConclusionBased on the outcomes of our study, implanting PRF into surgical extraction sites can be helpful in reducing postoperative pain and improving soft tissue healing. The use of PRF in extraction sockets is simple and biologically safe. The beneficial effects of PRF support its possible application in the field of dental surgery.

AESTHETIC AMELIORATION OF GINGIVAL RECESSION BY MODIFIED CORONALLY ADVANCED FLAP PROCEDURE: A 6 MONTHS FOLLOW-UP INTERVENTIONAL STUDY

Background:In recent times, aesthetics has become a major reason for the patients to seek the dentist. Gingival recession of the anterior teeth leads to long tooth appearance thereby hampering the aesthetics. The aim of the present study is to assess the efciency of modied coronally advanced ap (MCAF) technique in combination with platelet-rich brin (PRF) for recession coverage of Miller's class I and II cases. Materials and method: 10 patients with Miller's class I or class II gingiva were treated by modied coronally advanced ap (MCAF) technique in combination with platelet-rich brin (PRF). The clinical parameters such as gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), clinical attachment level (CAL), width of attached gingiva (WAG), thickness of attached gingiva (TAG) and the height of gingival recession (HGR) were recorded at baseline, 3 months and 6 months follow-up visits. Results: All the clinical parameters showed signicant improvement at 3 months and 6 months post-operatively when compared with the baseline values. The results obtained during the initial follow-up visit remained stable over a period of 6 months. Conclusion: MCAF is a foreseeable technique to achieve gingival recession coverage. Additionally, the use of PRF enables in improved WAG and TAG due to the release of growth factors.