Use Of Magnetic Nanoparticles Research Articles

Investigation of magnetic resonance contrast properties of PEG-coated gadolinium oxide nanoparticles in various biological environments

Abstract Magnetic nanoparticles (MNPs) are promising tools for biomedical applications, particularly in molecular imaging using magnetic resonance imaging (MRI). The unique magnetic properties of MNPs, combined with their similarity in size to biological objects, make them ideal candidates for in situ imaging probes. The present study explores the use of magnetic nanoparticles (MNPs) as contrast agents in magnetic resonance imaging (MRI) for improved diagnostic accuracy. Specifically, the study investigates the MR contrast properties of polyethylene glycol-coated gadolinium oxide nanoparticles (PEG@GONPs) in five different biological fluids. The nanoparticles were synthesized using the polyol route and their size, shape, and morphology were characterized using TEM, SEM, and FT-IR spectroscopy. The magnetic resonance (MR) relaxivity of PEG@GONPs was studied in different biologically relevant media, and results revealed highest relaxivity in plasma as compared to other media. In addition, comparative analysis of proton relaxivity of the synthesized nanoparticles was carried out with a well-known gadolinium-based contrast agent, Omniscan, in various medium. The present findings revealed that PEG@GONPs can serve as an effective contrast agent for MRI imaging in biological fluids such as plasma, which is crucial for preclinical diagnosis of specific diseases and lesions. The high relaxivity observed in plasma could be attributed to the interaction of the nanoparticles with plasma proteins, amplifying their magnetic properties which further improve their ability to produce contrast in MR images.

Synthesis of magnetic polystyrene nanocomposite by emulsion polymerization using a photo-responsive emulsifier for efficient crude oil absorption

Both human health and marine life are seriously threatened by crude oil spills into bodies of water. For effective crude oil spill cleaning, we created a magnetic polystyrene (m-PS) nanocomposite. The use of magnetic nanoparticles makes crude oil absorption more environmentally friendly by making it easier to collect and recycle using an external magnetic field. To make Fe3O4 nanoparticles compatible with the hydrophobic styrene monomer used in emulsion polymerization, they were treated using a hydrophobic surface modification reaction. This alteration facilitated the grafting of polystyrene (PS) chains onto the nanoparticles, which then underwent emulsion polymerization. As an emulsifier, a light-responsive amphiphilic block copolymer containing coumarin was created via reversible addition-fragmentation chain transfer polymerization. This allowed for regulated emulsification and demulsification in response to UV stimulation. The synthesized m-PS nanocomposite demonstrated a crude oil absorption capacity of up to 2.31 times of its own weight, indicating its high efficiency for crude oil spill cleanup. The synthetic emulsifier exhibited a significantly lower critical micelle concentration compared to the commercial P105 emulsifier (0.0976 against 0.354 mg/mL, respectively), indicating higher efficiency and reduced environmental impact. For a more thorough comprehension of the reported results, we also assessed the Hofmeister effect in PS produced using commercial and synthetic emulsifiers.

REVIEW OF LITERATURE ON NANOSCALE TOPOGRAPHIC MODIFICATION, MAGNETIC NANOPARTICLES, AND THEIR ROLES IN OSSEOINTEGRATION AND BONE TISSUE ENGINEERING

Here, we will review together the roles of nanoscale modification of titanium substrates and use of magnetic nanoparticles in osseointegration and bone tissue engineering. The technological advances in nanotechnology that has been achieved create new measures to increase the implants-biological systems for an optimal osseointegration through improving important cell-specific functions. We systematically review nanoscale surface modification techniques of titanium implants, covering their physicochemical properties, influence on cellular behaviors and clinical relevances. The report also lengthy discusses the clinical applicability of magnetic nanoparticles for drug and gene delivery systems, targeted cancer therapy as well bone tissue engineering regulting their intrinsic superparamagnetism properites, biocompatibility with other therapies etc. We present detailed review of these technologies and their potential applications in clinical settings.

Use of magnetic nanoparticles of iron oxide and their derivatives in the adsorption of rhodamine 6G and rhodamine B dyes

Rhodamines are cationic dyes widely used in textiles and as laser media, despite their many severe health and environmental hazards. In this work, we have assessed the potential of magnetite and cobalt ferrite nanoparticles (NPs) for removing rhodamines B (Rh B) and 6G (Rh 6G) from aqueous solutions. The magnetite (Fe3O4) NPs were synthesized by the co-precipitation method, while the cobalt ferrite (CoFe2O4) NPs were obtained hydrothermally. The structures of cubic magnetite Fe3O4 and cubic CoFe2O4 were confirmed by XRD and by FTIR and Raman, while the surface morphology checked by SEM showed clusters of polydisperse particles with average diameters around 51.0 nm and 57.0 nm, respectively. These particles presented calculated BET surface areas of 33.8 m2/g (Fe3O4) and 47.3 m2/g (CoFe2O4). Hysteresis curves confirmed the ferromagnetic behavior for both NPs. The point of zero charge was 6.46 for Fe3O4 and 7.16 for CoFe2O4, and using pH 4 in the system, the overall percent removal (%R) were above 80 % for all samples during the uptake of Rh B and 6G. The system Rh 6 G-CoFe2O4 performed better at pH 6 (%R ∼93 %). In all cases, the Elovich and pseudo-second-order (PSO) models were chosen for fitting the kinetic data, and after equilibrium was reached (∼ 30 min), the adsorptive capacities were estimated to be above 138 mg g−1 for all systems. Adsorption isotherms at 301, 311 and 321 K were obtained. Good agreement between the experimental data and the Langmuir, Freundlich and Sips models was observed for Rh 6 G adsorption by both NPs (R2 ≥ 0.90). The Freundlich model was the only one that fitted well the experimental data for Rh B (R2 ≥ 0.90, for all systems). The thermodynamic parameters were also calculated from the isotherm data.

Harnessing Magnetic Nanoparticles for the Effective Removal of Micro- and Nanoplastics: A Critical Review.

The spread of micro- (MPs) and nanoplastics (NPs) in the environment has become a significant environmental concern, necessitating effective removal strategies. In this comprehensive scientific review, we examine the use of magnetic nanoparticles (MNPs) as a promising technology for the removal of MPs and NPs from water. We first describe the issues of MPs and NPs and their impact on the environment and human health. Then, the fundamental principles of using MNPs for the removal of these pollutants will be presented, emphasizing that MNPs enable the selective binding and separation of MPs and NPs from water sources. Furthermore, we provide a short summary of various types of MNPs that have proven effective in the removal of MPs and NPs. These include ferromagnetic nanoparticles and MNPs coated with organic polymers, as well as nanocomposites and magnetic nanostructures. We also review their properties, such as magnetic saturation, size, shape, surface functionalization, and stability, and their influence on removal efficiency. Next, we describe different methods of utilizing MNPs for the removal of MPs and NPs. We discuss their advantages, limitations, and potential for further development in detail. In the final part of the review, we provide an overview of the existing studies and results demonstrating the effectiveness of using MNPs for the removal of MPs and NPs from water. We also address the challenges that need to be overcome, such as nanoparticle optimization, process scalability, and the removal and recycling of nanoparticles after the completion of the process. This comprehensive scientific review offers extensive insights into the use of MNPs for the removal of MPs and NPs from water. With improved understanding and the development of advanced materials and methods, this technology can play a crucial role in addressing the issues of MPs and NPs and preserving a clean and healthy environment. The novelty of this review article is the emphasis on MNPs for the removal of MPs and NPs from water and a detailed review of the advantages and disadvantages of various MNPs for the mentioned application. Additionally, a review of a large number of publications in this field is provided.

Rationale of using magnetically sensitive biomaterials in bone tissue therapy: a review

INTRODUCTION. Currently, new biomaterials are being intensively developed to improve the efficiency of repair of damage to hard and soft tissues. New approaches and methods for functionalizing biomaterials have been proposed. One such method is the use of magnetic nanoparticles. This approach is new and still little studied, however, the annual increase in the number of publications on this topic indicates the promise of studying the osteogenic effect of magnetic nanoparticles. AIM. To summarize the results of current research devoted to studying the effect of magnetically sensitive biomaterials on the functional activity of cells involved in the reparation of bone tissue damage. MATERIALS AND METHODS. A literature review was conducted using the databases PubMed and Scopus. Keywords used to conduct the search: electromagnetic field, magnetic nanoparticles, biomaterials, osteoinduction, bone regeneration. Request dates: February-March 2024, publication period 2000–2024 years. MAIN CONTENT. New approaches and methods for functionalizing biomaterials have been proposed. One such approach is the use of magnetic nanoparticles (MNPs). Traditionally, in medicine, MNPs are used as a contrast agent to improve the visualization of cancer tumors; in addition, MNPs can act as a matrix in targeted drug delivery systems and in hyperthermic therapy of cancer tumors. New experimental data show that the use of MNPs as a magnetically sensitive component in biomaterials is a promising way to stimulate the repair of bone defects and fractures. It has been shown that biomaterials modified by nanoparticles stimulate osteogenic differentiation of stem cells, increase proliferative activity and secretion of extracellular matrix proteins by bone cells. CONCLUSION. Integration of MNPs with organic and synthetic polymers, and other biomimetic constructs is a promising direction for creating osteogenic biomaterials for medical use, including those aimed at increasing the efficiency of regeneration of bone defects. The use of magnetically sensitive biomaterials makes it possible to create “smart” tissue-engineered structures controlled by external electromagnetic stimulus.

Magnetically Controlled Intraocular Delivery of Dexamethasone Using Silica-Coated Magnetic Nanoparticles.

Although the number of patients with eye diseases is increasing, efficient drug delivery to the posterior segment of the eyeball remains challenging. The reasons include the unique anatomy of the eyeball, the blood-aqueous barrier, the blood-retina barrier, and drug elimination via the anterior chamber and uveoscleral routes. Solutions to these obstacles for therapeutic delivery to the posterior segment will increase the efficacy, efficiency, and safety of ophthalmic treatment. Micro/nanorobots are promising tools to deliver therapeutics to the retina under the direction of an external magnetic field. Although many groups have evaluated potential uses of micro/nanorobots in retinal treatment, most experiments have been performed under idealized in vitro laboratory conditions and thus do not fully demonstrate the clinical feasibility of this approach. This study examined the use of magnetic nanoparticles (MNPs) to deliver dexamethasone, a drug widely used in retinal disease treatment. The MNPs allowed sustainable drug release and successful magnetic manipulation inside bovine vitreous humor and the vitreous humor of living rabbits. Therefore, controlled drug distribution via magnetic manipulation of MNPs is a promising strategy for targeted drug delivery to the retina.

A Short Appraisal of Magnetic Nanoparticles for Breast Cancer: In vitro and In vivo Research.

The increasing incidence of breast cancer and the associated morbidity due to higher metastasis created the urge to develop a nanocarrier that can be used as a potent therapeutic carrier with targeting efficacy. The use of superparamagnetic nanoparticles in breast cancer research and treatment has gained considerable attention in recent years. Magnetic nanoparticles (MNPs) can be used to construct nanocarriers since they possess superior properties such as superparamagnetism, easy surface functionalization to attach ligands, and non-immunogenic. MNPs are superior carriers that are used to target cancer cells without harming the normal cells in the body, which leads to therapeutic efficacy in the body. Along with their established anticancer potential and enhanced drug concentration at endosomal pH, the superparamagnetic property of MNPs was further exploited for their applications in reticuloendothelial uptake, drug delivery, medical imaging, and theranostics applications in breast cancer. Moreover, the clinical translational of MNPs, along with future prospects and key challenges in vivo, have been duly presented in the final review. The scientists preferred the ongoing research in MNPs due to their high biocompatibility and ease of targeting at molecular and cellular levels. The review highlighted the in vitro and in vivo research and patent supported data for potential use of MNPs for the treatment of breast cancer.

Gold Nanoparticle-Based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from Bovine Fecal Samples.

Current diagnostic methods for detecting foodborne pathogens are time-consuming, require sophisticated equipment, and have a low specificity and sensitivity. Magnetic nanoparticles (MNPs) and plasmonic/colorimetric biosensors like gold nanoparticles (GNPs) are cost-effective, high-throughput, precise, and rapid. This study aimed to validate the use of MNPs and GNPs for the early detection of Escherichia coli O157:H7, Salmonella enterica spp., Campylobacter jejuni, and Listeria monocytogenes in bovine fecal samples. The capture efficiency (CE) of the MNPs was determined by using Salmonella Typhimurium (ATCC_13311) adjusted at an original concentration of 1.5 × 108 CFU/mL. One (1) mL of this bacterial suspension was spiked into bovine fecal suspension (1 g of fecal sample in 9 mL PBS) and serially diluted ten-fold. DNA was extracted from Salmonella Typhimurium to determine the analytical specificity and sensitivity/LOD of the GNPs. The results showed that the CE of the MNPs ranged from 99% to 100% and could capture as little as 1 CFU/mL. The LOD of the GNPs biosensor was 2.9 µg/µL. The GNPs biosensor was also tested on DNA from 38 naturally obtained bovine fecal samples. Out of the 38 fecal samples tested, 81.6% (31/38) were positive for Salmonella enterica spp., 65.8% (25/38) for C. jejuni, 55.3% (21/38) for L. monocytogenes, and 50% (19/38) for E. coli O157:H7. We have demonstrated that MNP and GNP biosensors can detect pathogens or their DNA at low concentrations. Ensuring food safety throughout the supply chain is paramount, given that these pathogens may be present in cattle feces and contaminate beef during slaughter.

Ultra-fast in vivo directional dark-field x-ray imaging for visualising magnetic control of particles for airway gene delivery

Objective. Magnetic nanoparticles can be used as a targeted delivery vehicle for genetic therapies. Understanding how they can be manipulated within the complex environment of live airways is key to their application to cystic fibrosis and other respiratory diseases. Approach. Dark-field x-ray imaging provides sensitivity to scattering information, and allows the presence of structures smaller than the detector pixel size to be detected. In this study, ultra-fast directional dark-field synchrotron x-ray imaging was utlilised to understand how magnetic nanoparticles move within a live, anaesthetised, rat airway under the influence of static and moving magnetic fields. Main results. Magnetic nanoparticles emerging from an indwelling tracheal cannula were detectable during delivery, with dark-field imaging increasing the signal-to-noise ratio of this event by 3.5 times compared to the x-ray transmission signal. Particle movement as well as particle retention was evident. Dynamic magnetic fields could manipulate the magnetic particles in situ. Significance. This is the first evidence of the effectiveness of in vivo dark-field imaging operating at these spatial and temporal resolutions, used to detect magnetic nanoparticles. These findings provide the basis for further development toward the effective use of magnetic nanoparticles, and advance their potential as an effective delivery vehicle for genetic agents in the airways of live organisms.

Carboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine.

In the present study, an innovative and simple electrochemical magneto biosensor based on carboxyethylsilanetriol-modified iron oxide (Fe3O4) magnetic nanoparticles was designed for ultrasensitive and specific analysis of cotinine, an important marker of smoking. Anticotinine antibodies were covalently immobilized on carboxylic acid-modified magnetic nanoparticles, and the cotinine-specific magnetic nanoparticles created a specific surface on the working electrode surface. The use of magnetic nanoparticles as an immobilization platform for antibodies provided a large surface area for antibody attachment and increased sensitivity. In addition, the advantages of the new immobilization platform were reusing the working electrode numerous times, recording repeatable and reproducible signals, and reducing the necessary volume of biomolecules. The specific interaction between cotinine and cotinine-specific antibody-attached magnetic nanoparticles restricted the electron transfer of the redox probe and changed the impedimetric response of the electrode correlated to the concentration of cotinine. The magneto biosensor had a wide detection range (2-300 pg/mL), a low LOD (606 fg/mL), and an acceptable recovery (97.24-105.31%) in real samples. In addition, the current biosensor's measurement results were in good agreement with those found by the standard liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) methods. These results showed that a simple impedimetric immunosensing platform was generated for the cotinine analysis.

Application of Nanoparticles for Magnetic Hyperthermia for Cancer Treatment-The Current State of Knowledge.

Hyperthermia (HT) is an anti-cancer therapy commonly used with radio and chemotherapies based on applying heat (39-45 °C) to inhibit tumor growth. However, controlling heat towards tumors and not normal tissues is challenging. Therefore, nanoparticles (NPs) are used in HT to apply heat only to tumor tissues to induce DNA damage and the expression of heat shock proteins, which eventually result in apoptosis. The aim of this review article is to summarize recent advancements in HT with the use of magnetic NPs to locally increase temperature and promote cell death. In addition, the recent development of nanocarriers as NP-based drug delivery systems is discussed. Finally, the efficacy of HT combined with chemotherapy, radiotherapy, gene therapy, photothermal therapy, and immunotherapy is explored.

Thermomagnetic convection control strategies for electromagnetic devices immersed in a ferrofluid

This paper presents new control strategies for improving the thermomagnetic convection intensity in electromagnetic devices. The system consists of a winding prototype immersed in a ferrofluid solution based on magnetic nanoparticles. The whole system is exposed to an external magnetic field generated by an annular magnet placed against the tank. When possible, experimental results are compared with numerical ones obtained using the finite element method in a 2D-axisymmetric setting. The first control strategy consists of assessing the impact of Curie’s temperature on heat removal. Numerical calculations show that the maximum winding temperature decreases by 2.2°C with the use of magnetic nanoparticles with the lowest Curie temperature. Employing an auxiliary magnetic field may be another method for monitoring the thermomagnetic convection. The number of streamlines increases in the upper part of the tank due to the intensification of the magnetic force at the top of the winding. It is shown that magnetization, orientation, and location of the magnet play a crucial role in maximizing heat transfer inside the tank. The experiment shows a temperature decrease of 9 °C at the top of the winding if an external magnetic field is applied.

Magnetic Metallic Nanoparticles Coated with Carbon for the Catalytic Removal of Bromate from Water

Bromate, often detected in drinking water, is associated with a significant risk of cancer. Catalytic reduction has been recognized as an effective treatment technique to remove ions by reducing them over metal catalysts in the presence of a reducing agent, usually hydrogen. This work aims to synthesize metallic magnetic nanoparticles of iron oxide (FeO) and mixed iron oxides with manganese (MnFeO), cobalt (CoFeO), and copper (CuFeO) coated with carbon via chemical vapor deposition (C-MNP) to be applied as catalysts to the reduction of bromate in water. The use of magnetic nanoparticles coated with carbon enables catalyst recovery via magnetic separation and takes advantage of the catalytic properties of the carbon materials. The iron particles proved to be the most promising catalysts for the reduction of bromate into bromide, the highest removal being obtained with the CFeO@CVD750 sample, resulting in a 99% conversion after 120 min of reaction under the conditions tested. Due to its magnetic nature, the catalytic material was easily removed after the reaction and applied in four consecutive cycles without losing its catalytic properties. These results highlight the great potential of carbon-coated magnetic nanoparticles for reducing bromate in water.

Magnetic Nanoparticles: Synthesis, Characterization, and Their Use in Biomedical Field

In recent years, the use of magnetic nanoparticles (MNPs) in biomedical applications has gained more and more attention. Their unusual properties make them ideal candidates for the advancement of diagnosis, therapy, and imaging applications. This review addresses the use of MNPs in the field of biomedicine encompassing their synthesis, biofunctionalization, and unique physicochemical properties that make them ideal candidates for such applications. The synthesis of magnetic nanoparticles involves a range of techniques that allow for control over particle size, shape, and surface modifications. The most commonly used synthesis techniques that play a crucial role in tailoring the magnetic properties of nanoparticles are summarized in this review. Nevertheless, the main characterization techniques that can be employed after a successful synthesis procedure are also included together with a short description of their biomedical applications. As the field of magnetic nanoparticles in biomedical applications is rapidly evolving, this review aims to serve as a valuable resource, especially for young researchers and medical professionals, offering basic but very useful insights into recent advancements and future prospects in this highly interdisciplinary research topic.

Magnetic Nanocomposites for Removal of Arsenic from Water

Arsenic significantly impacts human health and the environment and its removal from wastewater is still difficult. Magnetic nanoparticles have come to light as a viable arsenic remediation technique, providing a fresh and long-lasting water purification method. This study investigates the use of magnetic nanoparticles to remove arsenic by concentrating on their adsorption mechanism, kinetics, potential for adsorption, recovery, and promising use of this method in the future. Due to the extensive surface area and variable surface chemistry of magnetic nanoparticles, they can effectively adsorb arsenic from water sources. Because their magnetic properties simplify separation and regeneration, they may be used again with little to no efficiency loss. As a result, they reduce trash output by providing an ecologically acceptable alternative to traditional adsorbents. The present study also examines the kinetics and adsorption process of magnetic nanoparticles, emphasising their improved selectivity and capacity for adsorption. Due to these characteristics, the authors were able to successfully remove arsenic from wastewater, resulting in better water quality and decreased health hazards after exposure to arsenic. Additionally, the potential applications of magnetic nanoparticles in removing arsenic have been highlighted. It is envisaged that advances in material science and nanotechnology will create unique magnetic nanoparticles with even better performance. Combining hybrid materials and surface alterations can increase their effectiveness in wastewater treatment settings.

Nanotechnology Innovations in Pediatric Cardiology and Cardiovascular Medicine: A Comprehensive Review.

(1) Background: Nanomedicine, incorporating various nanoparticles and nanomaterials, offers significant potential in medical practice. Its clinical adoption, however, faces challenges like safety concerns, regulatory hurdles, and biocompatibility issues. Despite these, recent advancements have led to the approval of many nanotechnology-based products, including those for pediatric use. (2) Methods: Our approach included reviewing clinical, preclinical, and animal studies, as well as literature reviews from the past two decades and ongoing trials. (3) Results: Nanotechnology has introduced innovative solutions in cardiovascular care, particularly in managing myocardial ischemia. Key developments include drug-eluting stents, nitric oxide-releasing coatings, and the use of magnetic nanoparticles in cardiomyocyte transplantation. These advancements are pivotal for early detection and treatment. In cardiovascular imaging, nanotechnology enables noninvasive assessments. In pediatric cardiology, it holds promise in assisting the development of biological conduits, synthetic valves, and bioartificial grafts for congenital heart defects, and offers new treatments for conditions like dilated cardiomyopathy and pulmonary hypertension. (4) Conclusions: Nanomedicine presents groundbreaking solutions for cardiovascular diseases in both adults and children. It has the potential to transform cardiac care, from enhancing myocardial ischemia treatment and imaging techniques to addressing congenital heart issues. Further research and guideline development are crucial for optimizing its clinical application and revolutionizing patient care.

Pair correlations of the easy magnetisation axes of superparamagnetic nanoparticles in a ferrofluid/ferrocomposite.

The widespread use of magnetic nanoparticles in modern technologies and medical applications highlights the need for reliable theoretical models that can predict their physical properties. The pair correlation function of two randomly selected superparamagnetic nanoparticles in a ferrofluid/ferrocomposite is studied to depict the joint probability density of the easy magnetisation axes across the planes of parameters of major importance; these are the interaction of ferroparticles with an external magnetic field, the energy of magnetic anisotropy inside the superparamagnetic nanoparticle, and the interparticle magnetic dipole-dipole interaction. Assuming the rotational symmetry of the system, we come to the conclusion that the pair correlations of interest are dependent only on the polar angles, determining the inclinations of the ferroparticle easy axes from the direction of an external magnetic field. The dimer configuration, where two ferroparticles are in close contact along a magnetic field with their easy magnetisation axes aligned, is the most probable. This configuration becomes more pronounced with increasing anisotropy energy, dipolar coupling constant, and external magnetic field.

Magnetic nanoparticles for use in bioimaging.

Magnetic nanoparticles (MNPs) are well-known contrast agents for use in medical imageology, facilitating disease detection via magnetic resonance imaging (MRI). With the development of nanotechnology, various MNPs have been exploited with strong contrast enhancement effects as well as multiple functions to conquer challenges related to the low detection accuracy and sensitivity. In this review, the typical characteristics and types of MNPs are outlined, and the design and fabrication of MNP-based MRI contrast agents as well as multi-mode imaging agents are also introduced by discussing the representative studies. In the pursuit of performance-enhanced MNPs, novel MNPs are expected to be developed as the next generation of contrast agents for precise bioimaging applications in a broad spectrum of fields.

Use of magnetic nanoparticles in an Ionic liquid (BMIM-PF6) based vortex‐assisted dispersive liquid–liquid microextraction (VSLLME) for measuring the traces amounts of cadmium in environmental, biological and nutritional samples

In the present project, the magnetic nanoparticles were used in a type of an ionic liquid based micro-extraction to measure trace amounts of cadmium in different real samples containing environmental water samples, saliva and human hair. In the optimum condition, the recovery percentages for the real sample were about 94.5% to 101.3%, showing the designed method is suitable to be used for analyzing the real environmental and biological samples. The main parameters were evaluated and optimized as follows: pH was 2.5, the volume of ionic liquid was 150 µl, the amount of Fe3O4 magnetic nanoparticles was 50mg, the volume of the complexing agent was 12 µl, and the vortex time was 1 min and 2 min for ionic liquid, and magnetic nanoparticle distribution steps, respectively. Also, the volume of the medium was 10 to 20 ml.