Use Of Plant Tissue Culture Research Articles

植物培養組織を用いたトロパンアルカロイド生合成の解析

植物培養組織を用いたトロパンアルカロイド生合成の解析

Further Approaches in the Production of Secondary Metabolites by Plant Tissue Cultures.

The production of useful compounds via plant tissue and organ culture systems has received increasing attention during recent years. Often applied tehniques are cell culture, organ culture and culture of genetically transformed cells and tissues. In most of the cases only low yields of the desired secondary metabolites were obtained by cell culture systems. Many efforts have been made to improve the productivity of the plant tissue cultures, such as studies on hormone-dependency, media composition or light effects. Examples of recent work in this field which may lead to a wider use of plant tissue culture are discussed.

Polyamines in growth and differentiation of plant cell cultures: the effect of nitrogen nutrition, salt stress and embryogenic media.

In vitro culture of plant cells and tissues has an increasingly important role in the advancement of both basic and applied aspects of plant growth and development. This includes the use of plant tissue cultures for the introduction of new traits by cell selection and genetic engineering, clonal micropropagation, pathogen elimination, as well as for elucidation of several molecular and metabolic events. Controlled organo-genesis and/or embryogenesis in cell and tissue cultures (i. e. regeneration of new plants) and selection of specific cell lines, are prerequisites for the practical utilization of the aspects mentioned above. Regeneration from tissue cultures is easily achieved in some plant species such as tobacco and carrot. Several agricultural crops and most woody plants are especially recalcitrant. In most cases growth of cell cultures and in vitro embryogenesis and organogenesis is manipulated by the use of known plant hormones only, in an empirical manner, and very little is known on the underlying mechanisms, and on the use of additional or alternative means which may regulate regeneration.