Urine Of Healthy Women Research Articles

Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women.

Two Gram-stain-positive strains, c9Ua_26_MT and c11Ua_112_MT, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus Limosilactobacillus. Phylogenetic analysis based on pheS gene sequences and core genomes showed that each strain formed a separated branch and are closest to Limosilactobacillus vaginalis DSM 5837T. The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_MT and the closest relative DSM 5837T were 90.7 and 42.9 %, respectively. The ANI and GGDC values between c11Ua_112_MT and the closest relative DSM 5837T were 91.2 and 45.0 %, and those among the strains were 92.9% and 51,0 %, respectively. The major fatty acids were C12 : 0 (40.2 %), C16 : 0 (26.7 %) and C18 : 1 ω9c (17.7 %) for strain c9Ua_26_MT, and C18 : 1 ω9c (38.0 %), C16 : 0 (33.3 %) and C12 : 0 (17.6 %) for strain c11Ua_112_MT. The genomic DNA G+C content of strains c9Ua_26_MT and c11Ua_112_MT was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_MT and c11Ua_112_MT represent two novel species of the genus Limosilactobacillus, for which the names Limosilactobacillus urinaemulieris sp. nov. (c9Ua_26_MT=CECT 30144T=LMG 31899T) and Limosilactobacillus portuensis sp. nov. (c11Ua_112_MT=CECT 30145T=LMG 31898T) are proposed.

Virus and bacterial associations verified in the urine of healthy subjects (pilot study)

Introduction. CCurrently, there are more and more new data that the urine of the healthy subject has its own unique microbiota and virobiota. Nevertheless, in the etiology and pathogenesis of the inflammatory diseases of the urinary system, the bacterial component is the most studied, but the viral component, as a rule, remains outside the scope of the standard clinical examination of patients. Objectives. To investigate the viral-bacterial associations in the urine of healthy subjects.Materials and methods. The 20 healthy sexually active women and men were examined, which are divided into groups according to gender: Group I – women (n = 10), Group II – men (n = 10). The average age of the subjects was 22.4 ± 1.2 years.Results. Bacteriological examination showed that in the urine of healthy women predominates Lactobacillus spp. (90,0%), Peptococcus spp. (80.0%), Propionibacterium spp. (70.0%), and in the urine of healthy men - Eubacterium spp. (70.0%) and Peptostreptococcus spp. (40.0%). During the polymerase chain reaction of urine were detected the papilloma (HPV) and herpetic (HSV) viruses in 40.0% of cases. In group I were verified HPV (20.0%) and HSVII (10.0%), in group II was found only the HPV (10.0%). In all cases, when viruses were detected in the urine, they were recorded as part of virus-bacterial associations. In one healthy woman in the urine were found HPV + HSVII associationsConclusions. The findings about of verification different taxa of viruses in the urine of healthy subjects are the basis for understanding and detailing the etiological structure of infections of the urinary system. The further studies should be aimed for increasing the cohort of healthy subjects in order to obtain the correct factograms of bacterial and viral patterns which present in their urine.

Simultaneous free and glycosylated pyridinium crosslink determination in urine: Validation of an HPLC-fluorescence method using a deoxypyridinoline homologue as internal standard

Pyridinoline (Pyr), deoxypyridinoline (D-Pyr), galactosyl-pyridinoline (Gal-Pyr) and glucosyl–galactosyl pyridinoline (GluGal-Pyr) are enzymatic mature pyridinium crosslinks. Generally, only total Pyr and D-Pyr urinary amounts (free + bound forms) are evaluated by HPLC as indices of bone resorption. This report describes the validation of an HPLC-fluorescence method for the simultaneous evaluation of free Pyr and D-Pyr, together with GluGal-Pyr and Gal-Pyr, in urine of healthy women ( n = 20, aged 27–41) and girls ( n = 20, aged 5–10). The use of an unnatural D-Pyr homologue, here proposed for the first time as internal standard, and of pure Pyr, D-Pyr, GluGal-Pyr and Gal-Pyr synthesized to be used as primary calibrators, guarantees method specificity and correct crosslink quantification. Urine, spiked with IS, was solid-phase extracted prior to HPLC analysis. Total Pyr and D-Pyr amounts were also evaluated after urine hydrolysis. The HPLC method was validated for selectivity, sensitivity, linearity, precision, accuracy, recovery and stability for all measured crosslinks. Both free and total Pyr and D-Pyr as well as GluGal-Pyr and Gal-Pyr amounts were significantly higher in girls than in women ( p < 0.0001), indicating an increased collagen turnover rather than only bone turnover. Gal-Pyr, for the first time evaluated in girls, was under its lower quantification limit (<LLOQ, <21.20 pmol/mL) in women. The measurement of free and glycosylated pyridinium crosslinks might provide more information on the degradation of various types of collagen than only that of total Pyr and D-Pyr. Moreover, this validated method could be a useful non-invasive technique for studying pathological conditions characterized by modified glycosylation enzyme activity and for more clinical investigation on bone fragility.

Prevalence and susceptibility pattern of methicillin- resistant Staphylococcus aureus isolates among healthy women in Zaria, Nigeria

Multi-resistant methicillin-resistant Staphylococcus aureus (MMRSA) has been commonly reported to be one of the commonest causes of nosocomial infections worldwide. Also, recent reports describe methicillin-resistant S. aureus (MRSA) carriage in persons in the community. The study investigated its prevalence in urine of healthy women and its susceptibility pattern to other antibiotics. Urine samples collected from healthy women volunteers in Zaria were cultured and screened for S. aureus using standard microbiological procedures. The isolates were then subjected to antibiotic susceptibility testing using disc diffusion technique. A total of 54 (36%) S. aureus isolates were isolated from 150 urine samples collected. The prevalence rate for married and single women was 31% and 46%, respectively. Of the S. aureus isolates, 37 (69%) were methicillin-resistant. The MRSA were highly resistant to ampicillin 100%, cephalexin 100%, clindamycin 92%, vancomycin 89% but had low resistance to pefloxacin 35%, ofloxacin 27% ciprofloxacin 27%, sparfloxacin 24% and gentamicin 16%. All the 37 (100%) MRSA isolates showed resistance to at least two antibiotics tested while 33 (89.2%) were multi-drug resistant.

Prevalence and susceptibility pattern of methicillinresistant Staphylococcus aureus isolates among healthy women in Zaria, Nigeria

Multi-resistant methicillin-resistant Staphylococcus aureus (MMRSA) has been commonly reported to be one of the commonest causes of nosocomial infections worldwide. Also, recent reports describe methicillin-resistant S. aureus (MRSA) carriage in persons in the community. The study investigated its prevalence in urine of healthy women and its susceptibility pattern to other antibiotics. Urine samples collected from healthy women volunteers in Zaria were cultured and screened for S. aureus using standard microbiological procedures. The isolates were then subjected to antibiotic susceptibility testing using disc diffusion technique. A total of 54 (36%) S. aureus isolates were isolated from 150 urine samples collected. The prevalence rate for married and single women was 31% and 46%, respectively. Of the S. aureus isolates, 37 (69%) were methicillin-resistant. The MRSA were highly resistant to ampicillin 100%, cephalexin 100%, clindamycin 92%, vancomycin 89% but had low resistance to pefloxacin 35%, ofloxacin 27% ciprofloxacin 27%, sparfloxacin 24% and gentamicin 16%. All the 37 (100%) MRSA isolates showed resistance to at least two antibiotics tested while 33 (89.2%) were multi-drug resistant.

Cross-Reaction with Luteinizing Hormone β-Core Is Responsible for the Age-dependent Increase of Immunoreactive β-Core Fragment of Human Chorionic Gonadotropin in Women with Nonmalignant Conditions

The beta-core fragment of human chorionic gonadotropin (hCGbetacf), also termed "beta-core" and urinary gonadotropin peptide (UGP), has been reported to be present in the urine of healthy women and to increase in concentration after menopause. This could reflect cross-reaction with the equivalent metabolite of luteinizing hormone (LH), the beta-LH-core. We measured immunoreactive LH, hCG, free alpha-subunit, and free beta-subunit hCG (hCGbeta), as well as beta-core, using the S504 RIA and Triton UGP enzyme immunoassay in 274 urine samples from women with nonmalignant gynecological conditions. The molar cross-reaction of each assay with purified beta-LH-core was determined. Cross-reaction with beta-LH-core was 100% in the LH and the S504 beta-core assay, 5% in the Triton UGP assay, and <0.1% in the hCG, free alpha-subunit, and free hCGbeta assays. Median urine concentrations of all analytes showed an age-dependent increase. LH and free alpha-subunit concentrations were approximately 10(3) pmol/mol creatinine; hCG and S504 beta-core were approximately 10(2) pmol/mol creatinine; free hCGbeta and Triton UGP beta-core were in the tens of pmol/mol creatinine. The S504 beta-core concentrations were 10% of those of LH. S504 beta-core was strongly correlated with LH, but not with hCG or with free hCGbeta (LH, r2 = 0.45; hCG, r2 = 0.26; free hCGbeta, r2 = 0.03). The concentrations of beta-core detected by the Triton UGP assay, which has a 5% cross-reaction with beta-LH-core, were 2% of LH and 5% of the S504 beta-core concentrations. Triton UGP values correlated strongly with LH concentrations, but less well with S504 beta-core, intact hCG, and free hCGbeta (LH, r2 = 0.44; S504 beta-core, r2 = 0.33; hCG, r2 = 0.32; free hCGbeta, r2 = 0.19). Immunoreactive beta-core in women free of malignancies reflects cross-reaction with concentrations of the metabolite of LH, beta-LH-core, within the health-related reference interval.

Effect of exercise on the renal clearance of amylase and lysozyme in humans.

1. Total protein amount and amylase and lysozyme activities were measured in the serum and urine of healthy women and men before and after strenuous exercise. 2. Exercise produced no major difference in the serum activities of amylase and lysozyme. 3. The renal clearance of amylase was unaffected by strenuous exercise, but total protein excretion was markedly increased. 4. Very high values of renal clearance of lysozyme were observed after exercise. This implies that the proximal tubular function of protein reabsorption was specifically affected.

Elimination of corticosteroids in the urine of healthy women

Es werden Ergebnisse quantitativchemischer Harncorticoidanalysen von gesunden Frauen mitgeteilt. Wahrend des Cyclus werden im Durchschnitt 880 γ/die mit dem Harn ausgeschieden. Im letzten Drittel der Graviditat betragt die durchschnittliche Ausscheidung 924 γ mit einer biologischen Streuung von ±350 γ. Im Wochenbett sinken die Harncorticoidwerte um 60 % ab. Die Corticoidausscheidung in der Menopause halt sich auf einem relativ konstanten Niveau von 520 γ/die.