Pantoea is an opportunistic bacterium primarily involved in nosocomial infections, impacting newborns and immunocompromised patients. The study aimed to investigate the virulence factor of Pantoea in urinary tract infections in hospitals in Al-Nasiriyah City, Iraq. The outcome appeared that 22 isolates out of 100 urine samples had been confirmed as Pantoea species by VITEK- 2. RcsA and hrpA genes were detected using conventional Polymerase Chain Reactions (PCR) and specific primers. The distribution of rcsA and hrpA was 40.9% and 59.1% respectively. The Pantoea species were distributed according to age, gender, and ABO blood groups. The percentage was 72.3% for ages 40–70, and females recorded 54.5% more than males. Individuals with the A blood group had a higher percentage (50%) than other blood groups; there were two types of variations (Transversion and Transition) of the16S rRNA gene. It was determined through the sequence alignment, and the symmetry was 90% with the Italian isolate of Pantoea species LL92 (ID: KF202812.1). These variations in the Pantoea local Iraqi isolate were registered in NCBI (ID: OM971851).This bacterial genus recently descended from the species of Enterobacter sp.. The isolation of Pantoea sp.was from UTIs, while previously, in most studies, they were isolated from the surrounding environment.