Unique Environment Research Articles

A Multi-Scale Feature Fusion Deep Learning Network for the Extraction of Cropland Based on Landsat Data

In the face of global population growth and climate change, the protection and rational utilization of cropland are crucial for food security and ecological balance. However, the complex topography and unique ecological environment of the Qinghai-Tibet Plateau results in a lack of high-precision cropland monitoring data. Therefore, this paper constructs a high-quality cropland dataset for the YarlungZangbo-Lhasa-Nyangqv River region of the Qinghai-Tibet Plateau and proposes an MSC-ResUNet model for cropland extraction based on Landsat data. The dataset is annotated at the pixel level, comprising 61 Landsat 8 images in 2023. The MSC-ResUNet model innovatively combines multiscale features through residual connections and multiscale skip connections, effectively capturing features ranging from low-level spatial details to high-level semantic information and further enhances performance by incorporating depthwise separable convolutions as part of the feature fusion process. Experimental results indicate that MSC-ResUNet achieves superior accuracy compared to other models, with F1 scores of 0.826 and 0.856, and MCC values of 0.816 and 0.847, in regional robustness and temporal transferability tests, respectively. Performance analysis across different months and band combinations demonstrates that the model maintains high recognition accuracy during both growing and non-growing seasons, despite the study area’s complex landforms and diverse crops.

Autonomous and Electric Vehicles in Urban Living Labs: Smart Mobility Strategies for the Future

The deployment of electric, connected, and autonomous vehicles on public roads presents a significant challenge that can be addressed through previously established frameworks developed globally for implementing these technologies as part of an urban living lab (ULL). This systematic review, based on records from four distinct databases, focuses on projects that have conducted deployments of self-driving technologies in streets within urban environments. The review describes relevant information about various initiatives, including a classification of the stages of development reached according to the urban area covered, safety considerations, and lessons learned for optimal deployment. On-board sensing technology, digital infrastructure, and energy and communication systems emerge as the essential components of a ULL with autonomous vehicles (AVs). A crucial goal for smart cities is ensuring the scalability of large-scale deployments of such ULLs for safe, clean, and future mobility experimentation. This can only be achieved through effective coordination among academia, government, industry, and society to guarantee the successful integration of multiple projects in a unique environment.

Synthesis, Characterization, and Biological Investigation of Antimony(III) Halide Complexes With Different Coordination Architectures Constructed From Thiophene Thiosemicarbazones

ABSTRACTIn this study, we report the synthesis, characterization, and biological investigation of antimony(III) halide complexes with various coordination architectures constructed from thiophene thiosemicarbazones. Antimony(III) thiophene‐2‐carbaldehyde thiosemicarbazone complexes (1, 2, 4, and 5) exhibit a square pyramidal geometry, with ligands coordinated to the central antimony atom in two distinct binding modes. In contrast, antimony(III) 2‐acetylthiophene thiosemicarbazone complexes (3, 6, and 7) adopt a seesaw geometry. These complexes (1–7) represent the first reported examples of antimony(III) halide thiosemicarbazone compounds. The unique coordination environments observed in these complexes are of significant importance within the realm of antimony chemistry. These synthesized complexes exhibit different coordination geometries as well as potential biological activities. The antiproliferative activity against the human breast adenocarcinoma (MCF‐7) cell line and antimicrobial activity against Gram‐positive and Gram‐negative bacteria were assessed. Antimony(III) thiophene‐2‐carbaldehyde thiosemicarbazone complexes (1, 2, 4, and 5) showed significant antiproliferative activity with IC50 values ranging from 8.5 to 19.1 μM, while antimony(III) 2‐acetylthiophene thiosemicarbazone complexes (3, 6, and 7) had higher IC50 values. Additionally, the antimony complexes demonstrated selective antimicrobial activity against Gram‐negative bacteria.

Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high-density growth.

Cyanobacteria provide a potential avenue for both biomanufacturing and combatting climate change via high-efficiency photosynthetic carbon sequestration. This study identifies novel photosynthetic organisms isolated from a unique geochemical environment and describes their genomes, growth behavior in culture, and biochemical composition. These cyanobacteria appear to make a tractable research model, and cultures are made publicly available alongside information about their culture and maintenance. Application of these organisms to carbon sequestration and/or biomanufacturing is discussed, including unusual, rapid settling characteristics of the strains relevant to scaled culture.

Biopsychosocial Health Considerations for Astronauts in Long-Duration Spaceflight: A Narrative Review.

Long-duration spaceflights beyond low-Earth orbit, including missions to the Moon and Mars, pose significant health risks. Although biomedical approaches commonly appear in the literature, considering psychological and social factors alongside physiologic health offers a more holistic approach to astronaut care. Integrating the biopsychosocial (BPS) framework into medical planning addresses complex spaceflight challenges and aids in developing mitigation strategies. This review examined health risks associated with long-duration spaceflight within a BPS framework. Sources included governmental space agencies, academic textbooks, and relevant publications from multiple databases. Considering the National Aeronautics and Space Administration's Human Research Program's 5 main hazards, a conceptual model was developed to highlight the multifactorial BPS effects of spaceflight. In space, astronauts face unique environments and biological adaptations, including fluid shift, plasma volume loss, bone density loss, and muscle atrophy. Noise and the absence of natural light disrupt circadian rhythms, causing sleep disturbances and fatigue, which affect physical and mental health. Studies on crews in isolated and confined extreme environments reveal psychosocial challenges, including impaired mood and cognition, interpersonal tension, and miscommunication. International collaboration in spaceflight introduces differences in communication, problem solving, and social customs due to diverse cultural backgrounds. Upcoming long-distance missions likely will amplify these challenges. This review emphasizes BPS health considerations in long-duration spaceflight. It highlights the interplay among psychological, social, and biological factors, advocating for multidisciplinary teams and a holistic approach to astronaut health and mission planning and the potential added value of BPS perspectives in considering countermeasures.

Comparative genomic analysis of two putative novel Idiomarina species from hypersaline miocene deposits

BackgroundHypersaline habitats, as extreme environments, are a great source of well-adapted organisms with unique properties as they have evolved various strategies to cope with these extreme conditions. Bioinformatics and genomic mining may shed light on evolutionary relationships among them. Therefore, the aim of this study was to assess the biodiversity and especially the strategies evolved within the Idiomarina genus, with the primary focus on the taxonomy and genomic adaptations of two novel strains affiliated with Idiomarina genus isolated from unique environment – brines of two Early Miocene salt deposits.ResultsBoth analyzed species belonged to the Idiomarina loihiensis cluster with similarity levels of 16S rRNA gene sequences as high as 99.5% and showed a significant genome size reduction, known characteristic of Idiomarina genomes, though within the genome of Sol25 strain the lowest extent of the carbohydrate utilization genes reduction was observed t among the Idiomarina species. Moreover, the comparative genome analyses indicated that despite both strains being isolated from geographically and geologically similar environments (brines from at least 12 Ma), the species showed higher relatedness to other Idiomarina species than to each other.ConclusionThe present findings highlighted the importance of genomic data in resolving taxonomic uncertainties and understanding of adaptation strategies of extremophiles. Geographic isolation likely contributed to population divergence of the Idiomarina genus, and the recent study offered insights into biogeographic patterns and allopatric speciation of this bacterial group.

Innovation in border regions: from inequality to equity! Starting with acknowledgment in policy

Abstract Living in a border area can create health inequalities due to limited access to healthcare compared to people in central regions. In centrally located areas, citizens can choose healthcare providers 360 degrees around them, benefiting from full prevention and health promotion coverage. In contrast, border residents have only 180 degrees of access, leading to partial inclusion in health services. National systems typically prioritize health equality but not necessarily equity, especially for those living in border regions. To address this gap, innovative policies that account for cross-border interactions are needed, benefiting over 35% of the EU population living in border areas. A key question is how to ensure health equity in border regions. To explore this, cross-border case studies, like air pollution, food purchases, antibiotic availability and q fever were looked at to identify the situation, the public health challenges it entails and existing solutions. The Essential Public Health Functions (EPHF) framework, developed by the World Health Organization and the International Association of National Public Health Institutes, was used to evaluate public health functions with a focus on achieving greater equity and inclusiveness. Currently in cross-border health key EPHF functions such as health promotion and disease prevention are emphasized, while others like public health governance and community participation need improvement. To better implement health equity in border regions, for use of the EPHF framework an additional and short guideline was developed on how border regions should be considered in national, regional, and local public health policies. This approach offers a pathway to addressing health inequalities in these unique environments: a crucial step towards creating health equity. Key messages • The EPHF framework as an innovative tool to strengthen public health in border regions. • Take the geographical living space of citizens in a border region into account to achieve health equity.

Deciphering decadal urban ozone trends from historical records since 1980.

Ozone pollution is a major environmental threat to human health. Timely assessment of ozone trends is crucial for informing environmental policy. Here we show that for the most recent decade (2013-2022) in the northern hemisphere, warm-season (April-September) mean daily 8-h average maximum ozone increases much faster in urban regions with top ozone levels (mainly in the North China Plain, 1.2±1.3 ppbv year-1) than in other, low-ozone regions (0.2±0.9 ppbv year-1). These trends widen the ozone differences across urban regions, and increase extreme pollution levels and health threats from a global perspective. Comparison of historical trends in different urban regions reveals that ozone increases in China during 2013-2022 differ in magnitude and mechanisms to historical periods in other regions since 1980. This reflects a unique chemical environment characterized by exceptionally high nitrogen oxides and aerosol concentrations, where reducing ozone precursor emissions leads to substantial ozone increase. Ozone increase in China has slowed down in 2018-2022 compared to 2013-2017, driven by ongoing emission reductions, but with ozone-favorable weather conditions. Historical ozone evolution in Japan and South Korea indicates that ozone increases should be suppressed with continuous emission reduction. Increasing temperature and associated wildfires have also reversed ozone decreases in the USA and Europe, with anthropogenic ozone control slowing down in recent decades.

Whole Genome Analysis Reveals Evolutionary History and Introgression Events in Bale Monkeys.

Background/Objective: The Bale monkey (Chlorocebus djamdjamensis) is a threatened primate species endemic to Ethiopia and, in contrast to other members of the genus Chlorocebus, lives at high altitudes and feeds mainly on bamboo. Two populations of the species are present, one in continuous bamboo forest (CF) in the eastern part of the species' range, and the other in fragmented forest (FF) in the western part. Based on mitochondrial DNA and phenotypic characteristics, previous studies have suggested introgression by parapatric congeners into the FF population but not into the CF population. The objective of this study was to gain insights into the evolutionary history of Bale monkeys and their potential genetic adaptations to high altitudes and for bamboo consumption. Methods: We sequenced the whole genomes of individuals from both populations and compared their genomes with those of the other five Chlorocebus species. We applied phylogenetic methods and conducted population demographic simulations to elucidate their evolutionary history. A genome-wide analysis was conducted to assess gene flow and identify mutations potentially associated with adaptations to high altitudes and for bamboo metabolism. Results: Our analyses revealed Bale monkeys as the sister clade to Chlorocebus aethiops and showed that gene flow occurred between C. aethiops and FF but not between C. aethiops and CF. In addition, we detected non-synonymous mutations in genes potentially associated with the adaptation to high altitudes (EPAS1) in both populations and with the adaptation for bamboo metabolism (TAS2R16, MPST, and TST) mainly in the CF population. Conclusions: Our study provides insights into the evolutionary history of a threatened primate species and reveals the genetic basis for its adaptions to unique environments and for diet specialization.

Analyzing and Strategizing the Emergency Language Landscape: A Case Study of Sanya City, China

The construction of language landscape for urban emergency services is an important part of public services. As a current city for the construction of Hainan Free Trade Port, Sanya City has a unique natural environment and is also a hotbed of various natural disasters in island cities. The challenges faced by emergency language services in Sanya City are not only how to effectively communicate emergency information to domestic citizens, but also how to cross language and cultural differences to ensure that all tourists can understand and follow emergency instructions. Against this background, it is of great significance to study the emergency language landscape of Sanya City.

ACSS2 regulates ferroptosis in an E2F1-dependent manner in breast cancer brain metastatic cells.

Brain metastasis diagnosis in breast cancer patients is considered an end-stage event. The median survival after diagnosis is measured in months, thus there is an urgent need to develop novel treatment strategies. Breast cancers that metastasize to the brain must adapt to the unique brain environment and are highly dependent on acetate metabolism for growth and survival. However, the signaling pathways that regulate survival in breast cancer brain metastatic (BCBM) tumors are not known. Primary brain tumor cells can convert acetate to acetyl-CoA via phosphorylation of acetyl-CoA synthetase 2 (ACSS2) by the cyclin-dependent kinase-5 (CDK5) regulated by the nutrient sensor O-GlcNAc transferase (OGT). Here, we show that breast cancer cells selected to metastasize to the brain contain increased levels of O-GlcNAc, OGT and ACSS2-Ser267 phosphorylation compared to parental breast cancer cells. Moreover, OGT and CDK5 are required for breast cancer cell growth in the brain parenchyma in vivo. Importantly, ACSS2 and ACSS2-S267D phospho-mimetic mutant are critical for in vivo breast cancer growth in the brain but not in the mammary fat pad. Mechanistically, we show that ACSS2 regulates BCBM cell survival by suppressing ferroptosis via regulation of E2F1-mediated expression of anti-ferroptotic proteins SLC7A11 and GPX4. Lastly, we show treatment with a novel brain-permeable small molecule ACSS2 inhibitor induced ferroptosis and reduced BCBM growth ex vivo and in vivo. These results suggest a crucial role for ACSS2 in protecting from ferroptosis in breast cancer brain metastatic cells and suggests that breast cancer brain metastatic cells may be susceptible to ferroptotic inducers.

FORMATION OF GENDER IDENTIFICATION IN THE PROCESS OF SOCIALIZATION OF ADOLESCENTS

The article describes the essence of gender identification in the process of socialization of adolescents. The social transformations taking place in modern society are changing the established ways of life, social norms, and ideological structures that define them, as well as attitudes toward them. These transformations have a significant impact on the formation of the individual, his or her status and position in society, professional development, and family roles. The purpose of the article is to substantiate the conditions for the formation of gender identification in the process of socialization of adolescents based on a gender approach, which is implemented in the family, school, and society. The concepts of gender and gender culture are highlighted. Attention is focused on the rapid increase in the percentage of so-called civil marriages, which lead to an increase in the number of single mothers and disorientation of sex education of children in the family. In these marriages, young people are not responsible for an “artificial” family, do not strive to have and raise children, and live together to satisfy their sexual needs. Relationships formed in this way are perceived and evaluated differently by boys and girls. Thus, among the 40 adolescent males surveyed, 70% were in favor of civil marriage, while 30% were against it. Among female adolescents, 31.5% approve of civil marriages, and 68.5% are against them. The results show that boys are more loyal to free, unencumbered relationships, but girls also perceive them quite favorably, not fully realizing that it is the woman who is socially and personally more vulnerable in such a situation. It has been found that adolescents show a high awareness of their personal identity through various aspects, such as gender perceptions, social roles, moral values, and interests. They are actively rethinking their place in society and their own views, which indicates their developed self-awareness and readiness to interact with the world around them. It is concluded that the rapid development of technology, diverse cultural influences, and new forms of communication create a unique environment in which gender norms and stereotypes are constantly being revised and evolved. This process is of particular importance for adolescents, as they are actively in the phase of self-determination and personal development. Understanding the gender aspects of social interaction among this group will help to identify and address the problems of inequality and discrimination at the initial stages of their occurrence. Prospects for further research of the problem are seen in the study of the influence of gender factors of adolescents on self-determination in modern society. Keywords: gender, identity, society, socialization, construct, personality, adolescents

Exploring Distributed Leadership in Special Schools (DLSS): Implications for Practice and Policy

ABSTRACT This research aims to understand distributed leadership (DL) in special school settings. It differs from existing studies that have been focused on mainstream school settings. Unlike prior research primarily focused on mainstream schools, this study explores these leadership structures within the unique environment of special schools. Consequently, it provides new insights into the implementation and effects of DL in this distinct educational context. The research is based on mixed methods research of convergent parallel design involving semi-structured interviews, questionnaires, examination of school policies, and focus group session. Special schools in the United Kingdom were used as case studies. Among other findings, the research found that common knowledge and experience were shared by practitioners in special schools. In addition, contrary to existing studies indicating that workload and pressure are key issues that are detrimental in mainstream schools, the findings of this research did not identify similar concerns. Furthermore, it found that special schools have higher numbers of Teaching Assistants (TA) with a certain degree of independence in their roles when compared to experiences reported in mainstream schools in existing literature. An implication of these findings for the management of special schools is the identification of a ready, competent and flexible workforce capable of delivering its objectives.

Botnets Unveiled: A Comprehensive Survey on Evolving Threats and Defense Strategies

ABSTRACTBotnets have emerged as a significant internet security threat, comprising networks of compromised computers under the control of command and control (C&C) servers. These malevolent entities enable a range of malicious activities, from denial of service (DoS) attacks to spam distribution and phishing. Each bot operates as a malicious binary code on vulnerable hosts, granting remote control to attackers who can harness the combined processing power of these compromised hosts for synchronized, highly destructive attacks while maintaining anonymity. This survey explores botnets and their evolution, covering aspects such as their life cycles, C&C models, botnet communication protocols, detection methods, the unique environments botnets operate in, and strategies to evade detection tools. It analyzes research challenges and future directions related to botnets, with a particular focus on evasion and detection techniques, including methods like encryption and the use of covert channels for detection and the reinforcement of botnets. By reviewing existing research, the survey provides a comprehensive overview of botnets, from their origins to their evolving tactics, and evaluates how botnets evade detection and how to counteract their activities. Its primary goal is to inform the research community about the changing landscape of botnets and the challenges in combating these threats, offering guidance on addressing security concerns effectively through the highlighting of evasion and detection methods. The survey concludes by presenting future research directions, including using encryption and covert channels for detection and strategies to strengthen botnets. This aims to guide researchers in developing more robust security measures to combat botnets effectively.

Environmental characterization and impact of climatic fluctuations on the diversity of extremophilic bacterial communities of a high-altitude seasonal meltwater pond on Ojos del Salado (Dry Andes, Chile)

Earth has unique environments where only microorganisms adapted to extreme conditions can survive. The focus of this study was a high-altitude seasonal meltwater pond on the Altiplano plateau at 5900 m (Dry Andes). The polyextremophilic bacteria of this habitat must adapt to a range of extremities, including cold and dry climate, high UV radiation, and low nutrient availability. A macro-scale analysis was performed to characterize the environment using satellite imagery. To study the composition of bacterial communities, samples were taken from permafrost, water, and sediment of a meltwater pond during three subsequent expedition years under different weather conditions (average, high precipitation, severe drought). The bacterial diversity of the samples was revealed by Illumina MiSeq 16S rRNA gene amplicon sequencing. The results showed that the relative abundance of dominant phyla, such as Pseudomonadota, Bacteroidota, Acidobacteriota, and Verrucomicrobiota varied according to sample types and weather conditions, supported by the satellite environmental state analysis that showed correlations between the availability of snow, water surfaces, and the corresponding surface temperature values. Strong moisture-dependent quantitative changes were detected in the phyla Cyanobacteriota and Actinomycetota. Predominant sequences were linked to orders Chitinophagales, Burkholderiales, Flavobacteriales, and Sphingomonadales, known for their ability to degrade various organic compounds.

Cellulose Acetates in Hydrothermal Carbonization: A Green Pathway to Valorize Residual Bioplastics.

Bioplastics possess the potential to foster a sustainable circular plastic economy, but their end-of-life is still challenging. To sustainably overcome this problem, this work proposes the hydrothermal carbonization (HTC) of residual bioplastics as an alternative green path. The focus is on cellulose acetate - a bioplastic used for eyewear, cigarette filters and other applications - showing the proof of concept and the chemistry behind the conversion, including a reaction kinetics model. HTC of pure and commercial cellulose acetates was assessed under various operating conditions (180-250 °C and 0-6 h), with analyses on the solid and liquid products. Results show the peculiar behavior of these substrates under HTC. At 190-210 °C, the materials almost completely dissolve into the liquid phase, forming 5-hydroxymethylfurfural and organic acids. Above 220 °C, intermediates repolymerize into carbon-rich microspheres (secondary char), achieving solid yields up to 23 %, while itaconic and citric acid form. A comparison with pure substrates and additives demonstrates that the amounts of acetyl groups and derivatives of the plasticizers are crucial in catalyzing HTC reactions, creating a unique environment capable of leading to a total rearrangement of cellulose acetates. HTC can thus represent a cornerstone in establishing a biorefinery for residual cellulose acetate.

Artificial intelligence in public service and governance in Nigeria

Purpose: This study explores the current state of artificial intelligence implementation in Nigeria’s public service and the potential benefits, challenges, and strategic steps needed to harness AI for improved governance and service delivery. Methods: The research design was qualitative. The data were collected using secondary data collection, in which a thorough literature review of academic articles, books, and reports related to AI was consulted. This study applied a thematic research approach to clarify the underlying issues, beliefs, and experiences related to artificial intelligence in governance and public services. The study was also anchored to content analysis. Results: The findings revealed that AI application in Nigeria’s public service is still in its early stages, with promising developments in areas such as e-governance, healthcare, banking sector, real estate business, and law enforcement/security outfits. There is a need for the government in Nigeria to invest significantly in infrastructural advancement and human capital development, which in turn will close the skill gaps, infrastructural deficits, and lapses that crop up from the unawareness of Artificial Intelligence in the technological advancement of Nigeria. Limitations: This study examined the current state of AI in Nigeria's public services and governance by identifying the key barriers that affect the adoption and implementation of AI. The study made progressive recommendations that integrated the application of artificial intelligence in public services and governance in Nigeria. Contributions: This study provides a comprehensive understanding of how AI can be adopted in Nigeria’s unique environment. Findings: This study did not receive any funding from any agency or organization.

Management of high grade primary cerebellar tumours.

Cerebellar high-grade gliomas (cHGG) are uncommon in adults, making up only about 1% of all high-grade gliomas. These tumours differ from supratentorial high-grade gliomas (sHGG) in terms of epidemiology, molecular traits, and the age of the patients. cHGG patients are typically younger and show a higher frequency of neurofibromatosis 1 (NF1) mutations, atypical RAS mutations, and H3K27M mutations. Standard treatment includes surgical resection followed by chemotherapy and radiation. Recent studies emphasize the genetic differences between cerebellar and supratentorial tumours, with new treatments targetting specific molecular abnormalities. Immunotherapy has shown limited effectiveness due to the unique tumour environment in cHGG, and further research is required to improve treatment strategies for these rare tumours.

Proposed Quality Metrics for Congenital Cardiac Anesthesia: A Scoping Review.

Congenital cardiac anesthesiologists practice in a unique environment with high risk for morbidity and mortality. Quality metrics can be used to focus clinical initiatives on evidence-based care and provide a target for local quality improvement measures. However, there has been no comprehensive review on appropriate quality metrics for congenital cardiac anesthesia to date. Members of the Quality and Safety Committee for the Congenital Cardiac Anesthesia Society proposed 31 possible candidate topics for metrics. Using a scoping review strategy, 3649 abstracts were reviewed with 30 articles meeting final criteria. Of these, 5 candidate metrics were unanimously proposed for local collection and national benchmarking efforts: use of a structured handover in the intensive care unit, use of an infection prevention bundle, use of blood conservation strategies, early extubation of cardiopulmonary bypass cases, and cardiac arrest under the care of a cardiac anesthesiologist. Many metrics were excluded due to a lack of primary data and perceived complexity beyond the scope of cardiac anesthesia practice. There is a need to develop more primary data including linking process measures with outcomes, developing risk-stratification for our patients, and collecting national data for benchmarking purposes.

Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight

Low Earth Orbit (LEO) has emerged as a unique environment for evaluating altered stem cell properties in microgravity. LEO has become increasingly accessible for research and development due to progress in private spaceflight. Axiom Mission 2 (Ax-2) was launched as the second all-private astronaut mission to the International Space Station (ISS). Frozen human induced pluripotent stem cells (hiPSCs) expressing green fluorescent protein (GFP) under the SOX2 promoter, as well as fibroblasts differentiated from SOX2-GFP hiPSCs, were sent to the ISS. Astronauts then thawed and seeded both cell types into commercially available 96-well plates, which provided surface tension that reduced fluid movement out of individual wells and showed that hiPSCs or hiPSC-derived fibroblasts could survive either in suspension or attached to a Matrigel substrate. Furthermore, both cell types could be transfected with red fluorescent protein (RFP)-expressing plasmid. We demonstrate that hiPSCs and hiPSC-fibroblasts can be thawed in microgravity in off-the-shelf, commercially-available cell culture hardware, can associate into 3D spheroids or grow adherently in Matrigel, and can be transfected with DNA. This lays the groundwork for future biomanufacturing experiments in space.