Unidentified Features Research Articles

Multilevel Dirichlet process mixture analysis of railway grade crossing crash data

This article introduces a flexible Bayesian semiparametric approach to analyzing crash data that are of hierarchical or multilevel nature. We extend the traditional varying intercept (random effects) multilevel model by relaxing its standard parametric distributional assumption. While accounting for unobserved cross-group heterogeneity in the data through intercept, the proposed method allows identifying latent subpopulations (and consequently outliers) in data based on a Dirichlet process mixture. It also allows estimating the number of latent subpopulations using an elegant mathematical structure instead of prespecifying this number arbitrarily as in conventional latent class or finite mixture models. In this paper, we evaluate our method on two recent railway grade crossing crash datasets, at province and municipality levels, from Canada for the years 2008–2013. We use cross-validation predictive densities and pseudo-Bayes factor for Bayesian model selection. While confirming the need for a multilevel modeling approach for both datasets, the results reveal the inadequacy of the standard parametric assumption in the varying intercept model for the municipality-level dataset. In fact, our proposed method is shown to improve model fitting significantly for the latter data. In a fully probabilistic framework, we also identify the expected number of latent clusters that share similar unidentified features among Canadian provinces and municipalities. It is possible thus to further investigate the reasons for such similarities and dissimilarities. This can have important policy implications for various safety management programs.

Analysis of extreme ultraviolet spectra from laser produced rhenium plasmas

Extreme ultraviolet spectra of highly-charged rhenium ions were observed in the 1–7 nm region using two Nd:YAG lasers with pulse lengths of 150 ps and 10 ns, respectively, operating at a number of laser power densities. The maximum focused peak power density was 2.6 × 1014 W cm−2 for the former and 5.5 × 1012 W cm−2 for the latter. The Cowan suite of atomic structure codes and unresolved transition array (UTA) approach were used to calculate and interpret the emission properties of the different spectra obtained. The results show that n = 4-n = 4 and n = 4-n = 5 UTAs lead to two intense quasi-continuous emission bands in the 4.3–6.3 nm and 1.5–4.3 nm spectral regions. As a result of the different ion stage distributions in the plasmas induced by ps and ns laser irradiation the 1.5–4.3 nm UTA peak moves to shorter wavelength in the ps laser produced plasma spectra. For the ns spectrum, the most populated ion stage during the lifetime of this plasma that could be identified from the n = 4-n = 5 transitions was Re23+ while for the ps plasma the presence of significantly higher stages was demonstrated. For the n = 4-n = 4 4p64dN-4p54dN+1 + 4p64dN−14f transitions, the 4d-4f transitions contribute mainly in the most intense 4.7–5.5 nm region while the 4p-4d subgroup gives rise to a weaker feature in the 4.3–4.7 nm region. A number of previously unidentified spectral features produced by n = 4-n = 5 transitions in the spectra of Re XVI to Re XXXIX are identified.

Seismic structure of the European upper mantle based on adjoint tomography

We use adjoint tomography to iteratively determine seismic models of the crust and upper mantle beneath the European continent and the North Atlantic Ocean. Three-component seismograms from 190 earthquakes recorded by 745 seismographic stations are employed in the inversion. Crustal model EPcrust combined with mantle model S362ANI comprise the 3-D starting model, EU00. Before the structural inversion, earthquake source parameters, for example, centroid moment tensors and locations, are reinverted based on global 3-D Green's functions and Frechet derivatives. This study consists of three stages. In stage one, frequency-dependent phase differences between observed and simulated seismograms are used to constrain radially anisotropic wave speed variations. In stage two, frequency-dependent phase and amplitude measurements are combined to simultaneously constrain elastic wave speeds and anelastic attenuation. In these two stages, long-period surface waves and short-period body waves are combined to simultaneously constrain shallow and deep structures. In stage three, frequency-dependent phase and amplitude anomalies of three-component surface waves are used to simultaneously constrain radial and azimuthal anisotropy. After this three-stage inversion, we obtain a new seismic model of the European curst and upper mantle, named EU60. Improvements in misfits and histograms in both phase and amplitude help us to validate this three-stage inversion strategy. Long-wavelength elastic wave speed variations in model EU60 compare favourably with previous body- and surface wave tomographic models. Some hitherto unidentified features, such as the Adria microplate, naturally emerge from the smooth starting model. Subducting slabs, slab detachments, ancient suture zones, continental rifts and backarc basins are well resolved in model EU60. We find an anticorrelation between shear wave speed and anelastic attenuation at depths 1, indicating predominantly horizontal flow within the upper mantle. In addition, subduction zones, such as the Apennines and Hellenic arcs, are characterized by vertical flow with ξ < 1 at depths greater than 150 km. We find that the direction of the fast anisotropic axis is closely tied to the tectonic evolution of the region. Averaged radial peak-to-peak anisotropic strength profiles identify distinct brittle-ductile deformation in lithospheric strength beneath oceans and continents. Finally, we use the ‘point-spread function’ to assess image quality and analyse trade-offs between different model parameters.

Из диалектологического архива: польская диалектная речь жителей окрестностей Бражуоле (Тракайский район, 1992 г.)

The article provides dialectological recordings of Polish (the so-called polszczyznа kresowа) from the Trakai region, specifically Bražuolė and its surroundings, made during the expeditions in the 1990’s organized by the Department of Slavic Philology at Vilnius University. The Polish recordings are from an areal characterized sociolinguistically by the interface of four languages: the Polish dialect, Belarusian (po-prostu), Russian, and Lithuanian. These recordings reflect crucial and previously unidentified features of the sociolinguistic environment, as well as constitute historical evidence of language contact in Bražuolė and its surroundings. The data presented in the article can be used for further work on the history of language contacts in Lithuania.

Sulphur-bearing species in molecular clouds

We study several molecules that could help in the solution of the missing sulfur problem in dense clouds and circumstellar regions, as well as in the clarification of the sulfur chemistry in comets. These sulfur molecules are: the trimer (CH2S)3 and the tetramer (CH2S)4 of thioformaldehyde, pentathian S5CH2, hexathiepan S6CH2, thiirane C2H4S, trisulfane HSSSH, and thioacetone (CH3)2CS. Infrared spectra of these species are calculated using density functional theory methods. The majority of calculated bands belong to the mid-infrared, with some of them occurring in the near and far-infrared region. We suggest that some of unidentified spectral features measured by Infrared Space Observatory in several active galactic nuclei and starburst galaxies could be caused by 1,3,5-trithiane ((CH2S)3), 1,3,5,7-tetrathiocane ((CH2S)4), and thiirane (C2H4S). The objects whose unidentified infrared features we compare with calculated bands are: NGC 253, M82, NGC 1068, Circinus, Arp 220, 30 Doradus, Orion KL, and Sgr B2.

Organic molecules in translucent interstellar clouds.

Absorption spectra of translucent interstellar clouds contain many known molecular bands of CN, CH+, CH, OH, OH+, NH, C2 and C3. Moreover, one can observe more than 400 unidentified absorption features, known as diffuse interstellar bands (DIBs), commonly believed to be carried by complex, carbon-bearing molecules. DIBs have been observed in extragalactic sources as well. High S/N spectra allow to determine precisely the corresponding column densities of the identified molecules, rotational temperatures which differ significantly from object to object in cases of centrosymmetric molecular species, and even the 12C/13C abundance ratio. Despite many laboratory based studies of possible DIB carriers, it has not been possible to unambiguously link these bands to specific species. An identification of DIBs would substantially contribute to our understanding of chemical processes in the diffuse interstellar medium. The presence of substructures inside DIB profiles supports the idea that DIBs are very likely features of gas phase molecules. So far only three out of more than 400 DIBs have been linked to specific molecules but none of these links was confirmed beyond doubt. A DIB identification clearly requires a close cooperation between observers and experimentalists. The review presents the state-of-the-art of the investigations of the chemistry of interstellar translucent clouds i.e. how far our observations are sufficient to allow some hints concerning the chemistry of, the most common in the Galaxy, translucent interstellar clouds, likely situated quite far from the sources of radiation (stars).

The PML domain of PML-RARα blocks senescence to promote leukemia.

In most acute promyelocytic leukemia (APL) cases, translocons produce a promyelocytic leukemia protein-retinoic acid receptor α (PML-RARα) fusion gene. Although expression of the human PML fusion in mice promotes leukemia, its efficiency is rather low. Unexpectedly, we find that simply replacing the human PML fusion with its mouse counterpart results in a murine PML-RARα (mPR) hybrid protein that is transformed into a significantly more leukemogenic oncoprotein. Using this more potent isoform, we show that mPR promotes immortalization by preventing cellular senescence, impeding up-regulation of both the p21 and p19(ARF) cell-cycle regulators. This induction coincides with a loss of the cancer-associated ATRX/Daxx-histone H3.3 predisposition complex and suggests inhibition of senescence as a targetable mechanism in APL therapy.

Coronene and pyrene (5, 7)-member ring defects

PAHs are known to be one of the carriers of the ubiquitous aromatic IR bands. The IR spectra of many objects show IR emission features derived from PAH molecules of different size. Still some of the characteristics of the emitting population remain unclear. The emission bands show details which cannot be explained so far. These unidentified IR features require further laboratory and observational investigations. We present a theoretical study of the IR spectra of PAHs containing (5,7)-member ring defects, focusing on pyrene and coronene. Using density functional theory, we investigate the effects of such defects on the IR spectra of pyrene and coronene and their cations and anions. In addition, we explore parts of the potential energy surface of the neutral species and discuss alternative formation pathways. The addition of (5,7)-membered ring defects in pyrene and coronene results in a change of the IR spectra, both molecules lose their typical spectroscopic signature. We find shifts in the positions of the band as well as different intensities and a rise in the number of features. The boundaries in terms of the size of the PAHs exhibiting a (5,7)-membered ring defect are studied and shown. Investigation of the minimal energy pathway leads to a result of 8.21 eV for pyrene and 8.41 eV for coronene as minimum activation barriers for the transformation from ground state to defected state. Whereas pyrene retains some of its symmetry due to the symmetry exhibited by the Stone-Wales defect itself, coronene loses much more of its symmetry. The formation of these (5,7)-ring defects in PAHs may be well supported in AGB stars or PNe. Those environments strongly enable the transition from the ground state to the defect state. Therefore the knowledge of the IR spectra of these molecules will support future investigations aiming for a thorough understanding of the unidentified IR emission bands.

CARBON-RICH DUST PAST THE ASYMPTOTIC GIANT BRANCH: ALIPHATICS, AROMATICS, AND FULLERENES IN THE MAGELLANIC CLOUDS

Infrared spectra of carbon-rich objects which have evolved off the asymptotic giant branch reveal a range of dust properties, including fullerenes, polycyclic aromatic hydrocarbons (PAHs), aliphatic hydrocarbons, and several unidentified features, including the 21 um emission feature. To test for the presence of fullerenes, we used the position and width of the feature at 18.7-18.9 um and examined other features at 17.4 and 6-9 um. This method adds three new fullerene sources to the known sample, but it also calls into question three previous identifications. We confirm that the strong 11 um features seen in some sources arise primarily from SiC, which may exist as a coating around carbonaceous cores and result from photo-processing. Spectra showing the 21 um feature usually show the newly defined Class D PAH profile at 7-9 um. These spectra exhibit unusual PAH profiles at 11-14 um, with weak contributions at 12.7 um, which we define as Class D1, or show features shifted to ~11.4, 12.4, and 13.2 um, which we define as Class D2. Alkyne hydrocarbons match the 15.8 um feature associated with 21 um emission. Sources showing fullerene emission but no PAHs have blue colors in the optical, suggesting a clear line of sight to the central source. Spectra with 21 um features and Class D2 PAH emission also show photometric evidence for a relatively clear line of sight to the central source. The multiple associations of the 21 um feature to aliphatic hydrocarbons suggest that the carrier is related to this material in some way.

Phosphorylation of hepatitis C virus RNA polymerases ser29 and ser42 by protein kinase C-related kinase 2 regulates viral RNA replication.

Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), an RNA-dependent RNA polymerase (RdRp), is the key enzyme for HCV RNA replication. We previously showed that HCV RdRp is phosphorylated by protein kinase C-related kinase 2 (PRK2). In the present study, we used biochemical and reverse-genetics approaches to demonstrate that HCV NS5B phosphorylation is crucial for viral RNA replication in cell culture. Two-dimensional phosphoamino acid analysis revealed that PRK2 phosphorylates NS5B exclusively at its serine residues in vitro and in vivo. Using in vitro kinase assays and mass spectrometry, we identified two phosphorylation sites, Ser29 and Ser42, in the Δ1 finger loop region that interacts with the thumb subdomain of NS5B. Colony-forming assays using drug-selectable HCV subgenomic RNA replicons revealed that preventing phosphorylation by Ala substitution at either Ser29 or Ser42 impairs HCV RNA replication. Furthermore, reverse-genetics studies using HCV infectious clones encoding phosphorylation-defective NS5B confirmed the crucial role of these PRK2 phosphorylation sites in viral RNA replication. Molecular-modeling studies predicted that the phosphorylation of NS5B stabilizes the interactions between its Δ1 loop and thumb subdomain, which are required for the formation of the closed conformation of NS5B known to be important for de novo RNA synthesis. Collectively, our results provide evidence that HCV NS5B phosphorylation has a positive regulatory role in HCV RNA replication. While the role of RNA-dependent RNA polymerases (RdRps) in viral RNA replication is clear, little is known about their functional regulation by phosphorylation. In this study, we addressed several important questions about the function and structure of phosphorylated hepatitis C virus (HCV) nonstructural protein 5B (NS5B). Reverse-genetics studies with HCV replicons encoding phosphorylation-defective NS5B mutants and analysis of their RdRp activities revealed previously unidentified NS5B protein features related to HCV replication and NS5B phosphorylation. These attributes most likely reflect potential structural changes induced by phosphorylation in the Δ1 finger loop region of NS5B with two identified phosphate acceptor sites, Ser29 and Ser42, which may transiently affect the closed conformation of NS5B. Elucidating the effects of dynamic changes in NS5B phosphorylation status during viral replication and their impacts on RNA synthesis will improve our understanding of the molecular mechanisms of NS5B phosphorylation-mediated regulation of HCV replication.

Characterization of melt and ejecta deposits of Kepler crater from remote sensing data

AbstractWe used Moon Mineralogy Mapper (M3), Arecibo and Mini‐RF radar, and Diviner radiometer data with Lunar Reconnaissance Orbiter (LRO) Camera and Kaguya Terrain Camera images to characterize the target, ejecta, and impact melt‐rich lithologies in and around lunar central peak crater Kepler. M3 data indicate the impact melt rocks of crater floor to be high‐Ca pyroxene dominated, distinct from the low‐Ca pyroxene‐dominated crater wall. The central uplift is high‐Ca pyroxene dominated, and has higher albedo. These observations are consistent with thin mare basalts underlain by noritic Imbrium ejecta, underlain by gabbroic crustal material. M3 data reveal an enigmatic, splash‐like feature of melt‐rich material on the southeastern (uprange) crater wall and flank. M3 data also highlight halos around Kepler. In detail the halos are slightly variable, but in broad terms they define a consistent feature, offset to the inferred downrange direction, and interpreted to reflect the distribution of glass‐bearing impact breccia. The radar data sets show most of the proximal ejecta to be radar‐bright. However, Diviner rock abundance data do not indicate the presence of blocks on the surface nor can they be seen using LRO Narrow Angle Camera images. Thus, the blocks giving rise to the enhanced radar signal are buried. Beyond the radar‐bright zone, a subtle radar‐dark halo emerges, coincident with a region of very low rock abundance in Diviner data. This multidisciplinary approach provides a robust analysis of the main characteristics of a lunar complex crater and reveals previously unidentified features related to the distribution of impact melt.

Core level studies of calcite and dolomite

Conventional X‐ray photoelectron spectroscopy (XPS) and synchrotron‐based X‐ray photoelectron spectroscopy (HRXPS) have been used to study Iceland spar calcite (CaCO3) and dolomite (CaMg(CO3)2). The obtained full widths at half maximum (FWHMs) are mostly narrower than in the previous results, which together with the symmetry of the fitted peaks indicate effective neutralisation of surface charging. Some previously unidentified features observed in the Ca 2p, C 1s and O 1s spectra of calcite have been suggested to be bulk plasmons. Also, surface core level shifts in Ca 2p (in calcite) and Mg 2p (in dolomite) spectra have been obtained and found to be consistent between XPS and HRXPS measurements. A peak attributed to carbide (CaC2) has been suggested to indicate beam‐assisted interaction with hydrocarbons found on the surface. Copyright © 2014 John Wiley &amp; Sons, Ltd.

Analysis of laser produced plasmas of gold in the 1–7 nm region

Extreme ultraviolet (EUV) spectra from gold laser produced plasmas were recorded in the 1–7 nm region using two Nd:YAG lasers with pulse lengths of 150 ps and 10 ns, respectively, operating at a range of power densities. The maximum focused peak power density was 9.5 × 1013 W cm−2 for the former and 5.3 × 1012 W cm−2 for the latter. Two intense quasicontinuous intensity bands resulting from n = 4–n = 4 and n = 4–n = 5 unresolved transition arrays dominate the 4–5.5 and 1.5–3.6 nm regions of both spectra. Comparison with atomic structure calculations performed with the Cowan suite of atomic structure codes as well as consideration of previous experimental and theoretical results aided identification of the most prominent features in the spectra. For the ns spectrum, the highest ion stage that could be identified from the n = 4–n = 5 arrays was Au28+ while for the ps plasma the presence of significantly higher stages was deduced and lines due to 4d104f–4d94f2 transitions in Ag-like Au32+ give rise to the strongest observed features within the n = 4–n = 4 array while in the n = 4–n = 5 array it was possible to identify a number of previously unidentified spectral features as resulting from 4f–5g transitions in the spectra of Au XX to Au XXXIII.

Rapid identification of plant materials by wooden-tip electrospray ionization mass spectrometry and a strategy to differentiate the bulbs of Fritillaria

The counterfeit plant products, especially by using incorrect plant materials in pharmaceutical industry, have become a global problem. The plant materials belonging to closely related species but differing in medicinal properties are difficult to be identified. Here, a novel and generally applicable approach to identify the sources of plant materials was developed, which was based on the use of wooden-tip electrospray ionization mass spectrometry (wooden-tip ESI-MS) and multivariate statistical analysis of unidentified MS features (non-targeted). Using this approach, six officinal species of Fritillariae Cirrhosae Bulbus had been successfully differentiated. In addition, Fritillariae Pallidiflorae Bulbus, a common adulterant of Fritillariae Cirrhosae Bulbus, was also identified by using the strategy reported here. Compared with DNA phylogenetic trees, our approach provided finer resolution in distinguishing the closely related Fritillaria species. By combining wooden-tip ESI-MS and multivariate statistical analysis, a useful method was developed here for rapid identification of the sources of herbs, which showed promising perspectives in tracking the supply chain of pharmaceutical suppliers.

Biochemical Diversity in the Trypanosoma congolense Trans-sialidase Family

Trans-sialidases are key enzymes in the life cycle of African trypanosomes in both, mammalian host and insect vector and have been associated with the disease trypanosomiasis, namely sleeping sickness and nagana. Besides the previously reported TconTS1, we have identified three additional active trans-sialidases, TconTS2, TconTS3 and TconTS4, and three trans-sialidase like genes in Trypanosoma congolense. At least TconTS1, TconTS2 and TconTS4 are found in the bloodstream of infected animals. We have characterised the enzymatic properties of recombinant proteins expressed in eukaryotic fibroblasts using fetuin as model blood glycoprotein donor substrate. One of the recombinant trans-sialidases, TconTS2, had the highest specific activity reported thus far with very low sialidase activity. The active trans-sialidases share all the amino acids critical for the catalytic reaction with few variations in the predicted binding site for the leaving or acceptor glycan. However, these differences cannot explain the orders of magnitudes between their transfer activities, which must be due to other unidentified structural features of the proteins or substrates selectivity. Interestingly, the phylogenetic relationships between the lectin domains correlate with their specific trans-sialylation activities. This raises the question whether and how the lectin domains regulate the trans-sialidase reaction. The identification and enzymatic characterisation of the trans-sialidase family in T. congolense will contribute significantly towards the understanding of the roles of these enzymes in the pathogenesis of Animal African Trypanosomiasis.

A Sensitive Spectral Survey of Interstellar Features in the Near-UV [3040-3700Å

AbstractWe present a comprehensive and sensitive unbiased survey of interstellar features in the near-UV range (3040-3700 Å). We combined a large number of VLT/UVES archival observations of a sample of highly reddened early type stars – typical diffuse interstellar band (DIB) targets. We stacked the individual observations to obtain a spectrum with a signal-to-noise ratio exceeding 1500. Careful inspection of this spectrum reveals tens of absorption features of interstellar nature, most of which can be identified with various atomic and molecular features. We furthermore detect four weak unidentified features, but we cannot establish their interstellar nature. Our sensitivity is limited by telluric and instrumental residuals; this precludes us from detecting broader features (e.g. DIBs). For each detected feature, we measured fundamental parameters (radial velocities, line widths, and equivalent widths). We also compare our co-added spectrum to cold gas-phase laboratory measurements of small, neutral polycyclic aromatic hydrocarbon (PAH) molecules.

Diffuse Interstellar Bands in (Proto-) Fullerene-Rich Environments

AbstractThe recent infrared detection of fullerenes (C60 and C70) in Planetary Nebulae (PNe) and R Coronae Borealis (RCB) stars offers a beautiful opportunity for studying the diffuse interstellar bands (DIBs) in sources where fullerenes are abundant. Here we present for the first time a detailed inspection of the optical spectra of the hot RCB star DY Cen and two fullerene PNe (Tc 1 and M 1-20), which permits us to directly explore the fullerenes - DIB connection. The DIB spectrum of DY Cen (García-Hernández et al. 2012a) is remarkably different from that in fullerene PNe (García-Hernández &amp; Díaz-Luis 2013). In particular, Tc 1 displays unusually strong 4428 Å and 6309 Å DIBs, which are normal (or not seen) in DY Cen. On the other hand, DY Cen displays an unusually strong 6284 Å DIB that is found to be normal in fullerene PNe. We also report the detection of new broad and unidentified features centered at 4000 Å and 6525 Å in DY Cen and Tc 1, respectively. We suggest that the new 4000 Å band seen in DY Cen may be related to the circumstellar proto-fullerenes seen at infrared wavelengths (García-Hernández et al. 2012a). However, the intense 4428 Å DIB (probably also the 6309 Å DIB and the new 6525 Å band) may be related to the presence of larger fullerenes (e.g., C80, C240, C320, and C540) and buckyonions (multishell fullerenes such as C60@C240 and C60@C240@C540) in the circumstellar envelope of Tc 1 (García-Hernández &amp; Díaz-Luis 2013).

THE DETECTION OF INTERSTELLAR ETHANIMINE (CH 3 CHNH) FROM OBSERVATIONS TAKEN DURING THE GBT PRIMOS SURVEY

We have performed reaction product screening measurements using broadband rotational spectroscopy to identify rotational transition matches between laboratory spectra and the Green Bank Telescope PRIMOS radio astronomy survey spectra in Sagittarius B2 North (Sgr B2(N)). The broadband rotational spectrum of molecules created in an electrical discharge of CH3CN and H2S contained several frequency matches to unidentified features in the PRIMOS survey that did not have molecular assignments based on standard radio astronomy spectral catalogs. Several of these transitions are assigned to the E- and Z-isomers of ethanimine. Global fits of the rotational spectra of these isomers in the range of 8 to 130 GHz have been performed for both isomers using previously published mm-wave spectroscopy measurements and the microwave measurements of the current study. Possible interstellar chemistry formation routes for E-ethanimine and Z-ethanimine are discussed. The detection of ethanimine is significant because of its possible role in the formation of alanine - one of the twenty amino acids in the genetic code.

THE DETECTION OF C60 IN THE WELL-CHARACTERIZED PLANETARY NEBULA M1-11

We performed multiwavelength observations of the young planetary nebula (PN) M1-11 and obtained its elemental abundances, dust mass, and the evolutionary status of the central star. The AKARI/IRC, VLT/VISIR, and Spitzer/IRS spectra show features due to carbon-rich dust, such as the 3.3, 8.6, and 11.3 um features due to polycyclic aromatic hydrocarbons (PAHs), a smooth continuum attributable to amorphous carbon, and the broad 11.5 and 30 um features often ascribed to SiC and MgS, respectively. We also report the presence of an unidentified broad feature at 16-22 um, similar to the feature found in Magellanic Cloud PNe with either C-rich or O-rich gas-phase compositions. We identify for the first time in M1-11 spectral lines at 8.5 (blended with PAH), 17.3, and 18.9 um that we attribute to the C60 fullerene. This identification is strengthened by the fact that other Galactic PNe in which fullerenes are detected, have similar central stars, similar gas-phase abundances, and a similar dust composition to M1-11. The weak radiation field due to the relatively cool central stars in these PNe may provide favorable conditions for fullerenes to survive in the circumstellar medium. Using the photo-ionization code CLOUDY, combined with a modified blackbody, we have fitted the ~0.1-90 um spectral energy distribution and determined the dust mass in the nebula to be ~3.5x10^{-4} Msun$. Our chemical abundance analysis and SED model suggest that M1-11 is perhaps a C-rich PN with C/O ratio in the gas-phase of +0.19 dex, and that it evolved from a 1-1.5 Msun star.

A specialized microvascular domain in the mouse neural stem cell niche.

The microenvironment of the subependymal zone (SEZ) neural stem cell niche is necessary for regulating adult neurogenesis. In particular, signaling from the microvasculature is essential for adult neural stem cell maintenance, but microvascular structure and blood flow dynamics in the SEZ are not well understood. In this work, we show that the mouse SEZ constitutes a specialized microvascular domain defined by unique vessel architecture and reduced rates of blood flow. Additionally, we demonstrate that hypoxic conditions are detectable in the ependymal layer that lines the ventricle, and in a subpopulation of neurons throughout the SEZ and striatum. Together, these data highlight previously unidentified features of the SEZ neural stem cell niche, and further demonstrate the extent of microvascular specialization in the SEZ microenvironment.