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Articles published on Uncinula necator

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  • Research Article
  • 10.1002/bip.70013
Deciphering the Synergistic Action of Irradiated Chitosan and Biocontrol Agents for the Management of Powdery Mildew in Grapevines.
  • Apr 11, 2025
  • Biopolymers
  • Komal R Shinde + 4 more

This study aimed to evaluate the synergistic action of electron beam irradiated chitosan and Ampelomyces quisqualis for the management of powdery mildew, the most significant disease incited by the obligate fungus Erysiphe necator Schw. (Formerly known as Uncinula necator (Schw.) Burr.) that causes substantial losses in grapes. Invivo field trials conducted during 2020-21 and 2021-22, the evaluation of irradiated chitosan and bioagent and fungicide for the efficient in managing the grape powdery mildew disease. The fungicide sulfur 80% WDG was determined to be the most efficient. However, it was followed by a friendly combination of irradiated chitosan (150 ppm) with A. quisqualis (0.5%). Eco-friendly molecules, that is irradiated chitosan 150 ppm with A. quisqualis (0.5%), were found to be the best alternative for chemical molecules to achieve the disease control63.60% and were identified alternative to chemical treatments to manage the powdery mildew disease of grapes. Irradiated chitosan and biocontrol agents showed synergistic action for the management of powdery mildew in grapevines.

  • Research Article
  • 10.21608/jpd.2025.410809
Integrated Management Of Grapes Powdery Mildew (Uncinula necator), In Nubaria Locations, And Its Effect On The Grapes Yield
  • Apr 1, 2025
  • Journal of Productivity and Development
  • Sahar Sharkawy + 6 more

Integrated Management Of Grapes Powdery Mildew (Uncinula necator), In Nubaria Locations, And Its Effect On The Grapes Yield

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  • Research Article
  • Cite Count Icon 2
  • 10.33093/jiwe.2025.4.1.8
Machine Learning Approaches for Detecting Vine Diseases: A Comparative Analysis
  • Feb 14, 2025
  • Journal of Informatics and Web Engineering
  • Waheed Ahmad + 4 more

This study investigates the classification of vine leaf diseases using convolutional neural networks (CNNs), focusing on three major diseases: powdery mildew, caused by fungus Uncinula necator, Red Blotches associated with pathogens such as Phomopsis viticola, Grapevine Leafroll Disease and leafroll associated Grape -linked virus (GLRaV). Accurate diagnosis of these high-risk diseases is critical to vine health and yields. We evaluated the performance of three CNN algorithms—MobileNetV2, ResNet50, and VGG16 —by comparing their training and validation accuracies, as well as loss over ten seasons. MobileNetV2 emerged as the most robust model, exhibiting high accuracy and low loss, indicating strong generalizability. ResNet50 showed a steady increase in accuracy, but with high variability, indicating that probabilities with complex models or extended training requirements VGG16 showed notable improvements in training accuracy but encountered difficulties it involves consistency during validation, which means overfitting. Although MobileNetV2 proved to be the most efficient for this task, our analysis suggests that replicating ResNet50 and VGG16 can improve their performance. Future research will explore longer training times, larger data sets, and other methods to further improve the generalizability and robustness of this model This work highlights the ability of CNN to detect vine leaves emphasize early diseases and provide a strategy for sustainable viticultural practices.

  • Research Article
  • 10.36495/phss.2024.70.135-146
Monitoring of the phytosanitary state of grape plantations in the south of Ukraine in 2022—2024
  • Dec 30, 2024
  • Interdepartmental Thematic Scientific Collection of Phytosanitary safety
  • Yu Klechkovskyi + 3 more

Goal. The protection of vineyards is based on monitoring, which provides early detection, assessment of the degree of infection and forecasting of future populations of pests, which increases the chances of avoiding economic losses from them. Phytosanitary monitoring in regulating the intensity of development and spread of phytopathogenic organisms is of undoubted economic and environmental importance. Methods of the work was to study the peculiarities of the spread and development of harmful objects and weeds in the vineyards of the South of Ukraine. The main research methods were: field — to determine the distribution, development and harmfulness of the main harmful objects of vineyards and weeds; laboratory — to study pathogens of grape diseases; visual and mathematical-statistical. The objects of monitoring were grape plantations of different planting dates and varietal assortment. Results. Field surveys in 2022—2024 revealed the spread and development of Plasmopara viticola Berl. et Toni and Uncinula necator Berk. on grape plantations in the South of Ukraine. It was found that the most favorable for Plasmopara viticola Berl. et Toni was the vegetation period of 2024, when a high degree of disease development was observed on the inflorescences and the spread in untreated areas was almost 80%, with the development of the disease 20%. It was determined that during the years of research the conditions were favorable for the manifestation of Uncinula necator Berk., the highest percentage of distribution was observed at the end of the growing season on the clusters and reached 60%, with 30% development. Pheromone monitoring conducted during the study period revealed the dynamics of Lobesia botrana Schiff. population development, the timing and duration of all stages of the pest. Observations showed a different number of males of the first generation of the butterfly per trap. The largest number of males, compared to 2023—2024, was recorded in 2022. It was found that a mixed type of weediness is observed in vineyards, namely, root and rhizome perennials, which are the most widespread and harmful weed species. Conclusions. The results of the research conducted in 2022—2024, taking into account the phytosanitary condition of vineyards in the following years, provide the basis for the development of a system of protective measures for further implementation in the technology of grape growing.

  • Research Article
  • 10.3389/fpls.2024.1439776
Biocontrol agents transform the stability and functional characteristics of the grape phyllosphere microenvironment.
  • Oct 16, 2024
  • Frontiers in plant science
  • Tao He + 11 more

The spread of grape leaf diseases has a negative impact on the sustainable development of agriculture. Diseases induced by Uncinula necator significantly affect the quality of grapes. Bacillus biocontrol agents have been proven effective in disease management. However, limited research has been conducted on the impact of biocontrol agents on the assembly and potential functions of plant phyllosphere microbial communities. This study used high-throughput sequencing combined with bioinformatics analysis and culture omics technology for analysis. The results showed that biocontrol bacteria B. subtilis utilized in this study can significantly reduce the disease index of powdery mildew (p<0.05); concurrently, it exhibits a lower disease index compared to traditional fungicides. A comprehensive analysis has revealed that biocontrol bacteria have no significant impact on the diversity of phyllosphere fungi and bacteria, while fungicides can significantly reduce bacterial diversity. Additionally, biocontrol agents can increase the complexity of fungal networks and enhance the degree of modularity and stability of the bacterial network. The results also showed that the biocontrol agents, which contained a high amount of B. subtilis, were able to effectively colonize the grapevine phyllosphere, creating a microenvironment that significantly inhibits pathogenic bacteria on grape leaves while enhancing leaf photosynthetic capacity. In conclusion, biocontrol agents significantly reduce the grape powdery mildew disease index, promote a microenvironment conducive to symbiotic microorganisms and beneficial bacteria, and enhance plant photosynthetic capacity. These findings provide a basis for promoting biocontrol agents and offer valuable insights into sustainable agriculture development.

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  • Research Article
  • Cite Count Icon 1
  • 10.31742/isgpb.84.1.10
Evaluation for powdery mildew resistance in grapevine (Vitis venifere L.) parental germplasm under in-vitro and natural field conditions
  • Apr 10, 2024
  • Indian Journal of Genetics and Plant Breeding (The)
  • M Ramya Sree + 7 more

Powdery mildew (PM) caused by Erysiphe necator Schwein [(syn. Uncinula necator (Schweinf.) Burrill.] is an ascomycete biotrophic fungus of grapevine, leading to substantial yield and economic losses in infected crop. Thirty-four genotypes of Vitis vinifera L., including commercial varieties and hybrids, two genotypes of V. champini, three interspecific hybrids of Vitis species and two wild indigenous Himalayan species, namely V. parviflora and V. jacquemontii were assessed for resistance to powdery mildew using the leaf disc method in-vitro with E. necator isolate and in the field under natural condition during 2022 and 2023. Significant differences in the scores among the grape genotypes were observed in both in-vitro and field conditions. Resistance evaluation under both conditions classified V. parviflora, 110R, Coudere 1613, Dogridge, St. George and V. vinifera cultivars like Male Hybrid, Pusa Navrang, Blank Prince and Merlot as resistant sources, which can serve as valuable donor parents for breeding programs. Correlation analysis illustrated the negative correlation between disease severity index (DSI) and temperature (r = -0.50 (max), r = -0.48 (min)), rainfall (r = -0.07) and a positive correlation with relative humidity (r = 0.42). In-vitro inoculation, Organisation Internationale de la Vigne et du Vin Descriptors (OIV) scores (7 days post inoculation (dpi)) showed a negative correlation with hyphal area percentage (r = -0.90) and a positive correlation with necrosis (r = 0.86) indicating the resistant genotypes showing necrosis at the infection site and thus restricted the pathogen growth

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  • Research Article
  • 10.3329/bjb.v53i1.72297
Identification of he-ne laser based mutation induced bacteria against grape disease
  • Mar 31, 2024
  • Bangladesh Journal of Botany
  • Ruimin Fu + 6 more

In the present study six bacterial strains from the soil of grape orchard were isolated and identified. Their antagonistic ability against Uncinula necator and Plasmopara viticola which cause downy mildew and powdery mildew of grapes, respectively were studied. Through phenotypic, physiological and biochemical studies, SF6 strain was identified as Bacillus subtilis which can effectively inhibit the growth of Uncinula necator and Plasmopara viticola of grapes. In order to enhance the antagonistic ability of the SF6 strain, mutation was induced by He-Ne laser radiation and, six mutant strains were obtained. Among the six mutant strains, SF66 showed the strongest antagonistic activity to Uncinula necator and Plasmopara viticola of grapes with its genetic stability. The finding of this research provides important impetus for the development of beneficial antagonistic microorganisms through He-Ne laser radiation breeding. Bangladesh J. Bot. 53(1): 33-40, 2024 (March)

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  • Research Article
  • 10.1051/e3sconf/202456303002
Damage periods of the main diseases occurring in vineyards during the annual development phases
  • Jan 1, 2024
  • E3S Web of Conferences
  • Asror Rakhmatov + 3 more

In the study, the researchers focused on understanding the developmental stages and damage periods of several key pathogens affecting vineyards. Gloeosporium ampelophagum Sacc, Uncinula necator Burrill, Agrobacterium tumefaciens Conn, Plasmopara viticola Berl &amp; De Toni, Cercospora vitis Sacc, and Botrytis cinerea Persoon are known to significantly impact vine health and grape yield. By analyzing the optimal periods of development for these pathogens, the researchers aimed to provide vineyard managers with valuable insights for implementing effective disease management strategies. Vineyard diseases pose a significant threat to grape production worldwide, affecting not only crop yield but also the quality of the final product. Gloeosporium ampelophagum, for example, causes anthracnose, leading to fruit rot and leaf lesions. Uncinula necator, the causal agent of powdery mildew, can reduce grape quality and yield if not controlled. Agrobacterium tumefaciens causes crown gall disease, affecting the vascular system of the vine and ultimately leading to reduced vigor and yield. Plasmopara viticola, responsible for downy mildew, can cause severe defoliation and reduce fruit quality. Understanding the developmental stages and damage periods of these pathogens is crucial for implementing timely and effective control measures. Integrated disease management strategies that combine cultural practices, biological controls, and chemical interventions can help mitigate the impact of these diseases. By identifying the optimal periods for disease development, vineyard managers can tailor their management practices to minimize the impact of these pathogens on vine health and grape production.

  • Research Article
  • 10.22268/ajpp-001244
The Effect of Foliar Spray Application with Salicylic Acid and Bacillus subtilis AB1 on The Growth and Protection of Grapevine Bushes from Powdery Mildew Disease Caused by the Fungal Pathogen Uncinula necator
  • Jan 1, 2024
  • Arab Journal for Plant Protection
  • Elham El Mosto + 2 more

El-Mosto, E., Z. Hassani and M. Abou Shaar. 2024. The Effect of Foliar Spray Application with Salicylic Acid and Bacillus subtilis AB1 on The Growth and Protection of Grapevine Bushes from Powdery Mildew Disease Caused by the Fungal Pathogen Uncinula necator. Arab Journal of Plant Protection, 42(3): 328-334. https://doi.org/10.22268/AJPP-001244 This study was conducted to evaluate the effect of foliar spray with three concentrations of salicylic acid (100, 200 and 300 mg/L), Bacillus subtilis AB1 at a concentration 2×106 cfu/ml, and the combined mutual effect of both, compared with a farmer's control on vegetative growth, clusters weight, productivity, and reduction of powdery mildew disease spread on grape vines. The growth length was significantly increased (140.7 cm) by applying the foliar spray of the bacteria + salicylic acid (200 mg/L) compared to the two treatments of salicylic acid 100 mg/L and the farmer's control (55.2 and 82.5 cm, respectively), whereas no significant differences were obtained between them and the rest of the treatments. The foliar spray with the treatment of both components (bacteria + salicylic acid 300 mg/L) was superior to all studied concentrations in relation to the average leaf surface area, clusters weight and productivity. All studied treatments reduced the severity of powdery mildew infection on leaves, except for salicylic acid at 200 mg/L (0.19) and no significant differences were recorded between all treatments (0.11-0.12) except for the two treatments (bacteria alone and farmer's control (0.15 and 0.14), respectively, in terms of reducing disease the severity of infection on fruit clusters. Keywords: Grapevine, salicylic acid, Bacillus subtilis AB1, vegetative growth, productivity, powdery mildew

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  • Research Article
  • Cite Count Icon 1
  • 10.46568/bios.v4i4.154
Optimizing Fungicide Sprays to Tackle Powdery Mildew (Uncinula necator) At The Right Time For healthy grapes production
  • Oct 10, 2023
  • BioSight
  • Mueen Uddin + 7 more

Introduction: Powdery mildew, caused by the fungus Uncinula necator, is a prevalent and harmful disease affecting grapevines, resulting in decreased fruit set and yield. This study aimed to evaluate the effectiveness of various fungicides and spray timings in managing U. necator in grape vineyards. Methods: A field trial was conducted in Pishin, Balochistan in 2020, employing a randomized complete block design with two factors: four fungicides (control, protective fungicide-Bordeaux mixture, curative fungicide-Elite 45 wp, systemic fungicide-Quintec) and four spray timings (dormant spray, bud break, one week before bloom, and berry formation). Results: The findings revealed significant variations in disease severity (PDI) among leaves, inflorescence, and bunches across different fungicides, spray timings, and their interactions. Application of the protective fungicide one week before bloom proved highly effective in preventing U. necator infection, resulting in the lowest PDI values for leaves (0.44%), inflorescence (0.67%), and bunches (0.0%). Curative fungicides sprayed at bloom stage also reduced PDI for inflorescence (2.17%) and bunches (3.56%). Systemic fungicides applied during berry formation exhibited lower PDI for inflorescence (6.44%) and bunches (4.0%) compared to other fungicides. The highest grape production (27.10 t ha-1) was achieved with the protective fungicide sprayed at bloom stage, followed by the curative fungicide at bloom stage (25.87 t ha-1). The negative and highly significant relationship (R2=0.809) between PDI of leaves and grape yield indicated that higher disease severity led to decreased yield. Conclusion: In conclusion, a protective fungicide spray before bloom, followed by a systemic fungicide spray at berry formation, effectively controls U. necator and ensures healthier and higher grape yields.

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  • Research Article
  • 10.31018/jans.v15i3.4654
Antimicrobial nature of specific compounds of Ampelomyces quisqualis identified from gas chromatography-mass spectrometry (GCMS) analysis and their mycoparasite nature against powdery mildew of grapes
  • Sep 19, 2023
  • Journal of Applied and Natural Science
  • Ranjan Kumar Jena + 7 more

Grapevine powdery mildew is the world's most important plant disease, and Ampelomyces frequently fight them. While it does not usually cause plant death, its major infections can result in significant production losses and severely impact wine quality. Fungicides are frequently used to control the disease, which can have long-term adverse effects on the ecosystem. As a result, alternative and environmentally friendly disease management approaches must be developed. The study aimed to reduce costly and toxic fungicide use by using Ampelomyces, a natural biofungicide, against various powdery mildew fungi. GC-MS analysis was also used to determine the antagonistic potential and efficacy of volatile organic chemicals produced by several Ampelomyces spp. against Erysiphe necator, which causes powdery mildew of grapes. The molecular characterization of A. quisqualis isolates based on using rDNA ITS region was also carried out and sequenced. GC-MS analysis identified various antimicrobial compounds, such as squalene (4.643%), octadecanoic acid (3.862%), tetradecanoic acid (3.600%), and 9,12-octadecadienoic acid (Z,Z) (1.451%). The least abundant compounds were 2-Hexadecanol, 1-Tricosanol, and 2-propenyl ester, with percentages of 0.485, 0.519, and 0.560, respectively. These bioactive compounds revealed by GC-MS analysis in crude extracts of A. quisqualis had a stronger antifungal and antibacterial activity against E. necator. As a result, using A. quisqualis to control the powdery mildew of grapes significantly reduced pathogen growth and disease incidence.

  • Research Article
  • Cite Count Icon 1
  • 10.30574/ijsra.2023.9.2.0637
Chabazitic zeolite in the cultivation and spray protection of Vitis vinifera
  • Aug 30, 2023
  • International Journal of Science and Research Archive
  • Domenico Prisa

Research objective: This article aims to highlight how chabazite zeolite can lead to improved growth and protection of vine plants, in particular the benefits it can bring to crops in terms of plant development, reduced incidence of fungal diseases and pesticide reduction. Materials and Methods: The experiments, which began in January 2023, were conducted in the CREA-OF greenhouses in Pescia (Pt), Tuscany, Italy (43°54′N 10°41′E) on Vitis vinifera cv 'Moscato giallo' and 'Passerina' plants. The plants were placed in pots with a diameter of 16, 50 plants per 3 replications, for a total of 150 seedlings per experimental thesis. The first trial on Vitis vinifera involved the following theses (irrigated and fertilized): i) peat 70% + pumice 30%; ii) peat 70% + pumice 20% + zeolite chabazite 10%; iii) peat 70% + pumice 10% + chabazite zeolite 20%. The second trial included spray treatments with micronized zeolite on the leaves to assess the control of diseases such as Botrytis cinerea, Oidium tuckeri and downy mildew. The trial included the following theses (irrigated and fertilized): · Control with treatment with water sprayed on the leaves every 10 days; · Control with copper-based treatment 1 kg/hl (copper oxychloride + lime) + folpet + sulphur 200g/hl, every 10 days; · Treated with chabazite zeolite 1.5 kg/hl + 250 g/hl (copper oxychloride) + sulphur, every 10 days. On 17 July 2023, plant height, vegetative weight, root volume and length, leaf area and the number of microorganisms in the substrate were determined. The number of plants affected by Botrytis cinerea, Oidium tuckeri and downy mildew was also assessed. Results and Discussion: The experiment showed that the use of zeolite has chabazite added to the cultivation substrate at the rate of 10-20% can effectively improve the vegetative and root growth of Vitis vinifera. Furthermore, when micronized chabazite zeolite is sprayed on the leaves, it can better contain diseases such as Botrytis cinerea, Oidium tuckeri and Downy mildew, compared to the use of copper and sulphur alone. According to the researchers, they have extensively researched chemicals that have excellent antifungal activity against a wide variety of fruit and vegetable diseases but can also be safely applied to crops. This has led them to find out that copper-containing zeolites have excellent fungicidal activity while exhibiting no problematic phytotoxicity. Plant pathogenic fungi are controlled by using crystalline zeolite (in particular chabazite), an agricultural and horticultural fungicide. Conclusions: In recent years, rapid population growth and urbanization have limited the agricultural area's ability to produce more crops. To perform more efficient agricultural activities, higher nutrient application rates are needed and more efficient water irrigation systems must be used. The problem, however, is that a substantial amount of nutrients might be washed out, polluting water resources and reducing product yields if high rates of fertilizers are applied to soils. For this reason, a cost-effective, pervasive, and green solution is essentially required to increase crop yields. Zeolites have been extensively investigated to enhance agricultural productivity.

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  • Research Article
  • Cite Count Icon 2
  • 10.23910/1.2023.3363
Strategic Management of Powdery Mildew of Grapes Using Fungicides and Bio-Control Agents
  • Jul 22, 2023
  • International Journal of Bio-resource and Stress Management
  • Sujoy Saha + 3 more

The study was carried out at ICAR-NRCG, Pune, Maharashtra, India during November–February of 2019–2020 and 2020–2021 to evaluate the bio-efficacy of fungicides and biocontrol agents and level of tolerance against powdery mildew of grapes. Total 18 treatments categorised into three groups were taken into account. Three groups viz. fungicides, biocontrol agents (Bacillus licheniformis, Trichoderma afroharzianum, Ampelomyces quisqualis) along with metrafenone and commercial formulations of biocontrol agents were analysed for disease control. A total of five applications of each treatment were made during the growing season. Results showed that, in case of Group I (registered fungicides), Hexaconazole 5 EC @ 1 ml l-1/ Difenoconazole 25 EC @ 0.5 ml l-1/Myclobutanil 10 WP @ 0.4 g l-1/Two sprays of sulphur @ 2 g l-1 was most effective in controlling the disease. In case of pure culture, Trichoderma afroharzanium had proved to be most effective in the control of powdery mildew when sprayed in alteration with Metrafenone. In Group III, alternate applications of commercial formulations of Bacillus subtilis (2 ml l-1), Trichoderma viridae (2 g l-1), Ampelomyces quisqualis (5 ml l-1) and sulphur (2 g l-1) was most effective in controlling the disease. It was concluded that the application of bio-control agents in alternation with chemical fungicides controlled the disease effectively.

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  • Research Article
  • 10.20870/oeno-one.2023.57.3.7064
Investigation of powdery &amp; downy mildew segregation in Bogazkere hybrids of Turkish wine grape
  • Jul 4, 2023
  • OENO One
  • Metehan Gunhan + 3 more

Powdery mildew (Erysiphe necator syn. Uncinula necator) and downy mildew (Plasmapora viticola) are the most harmful diseases to grapevine production in Turkey. The aim of this study was to generate hybrids between the susceptible Turkish wine grape variety ‘Bogazkere’ and powdery and downy mildew resistant microvine breeding lines and evaluate their resistance to these important diseases. Marker Assisted Selection (MAS) was used to determine the inheritance of powdery and downy mildew resistance and flower sex loci of the 36 F1 progeny plants obtained by cross-pollination at genotypic level. Based on the PCR results, phenotyping and leaf bioassay were performed to validate initial MAS for 11 and 9 representative progeny with four resistant and susceptible controls. As a result, two tall powdery and downy mildew-resistant vines, three powdery mildew-resistant microvines and two downy mildew-resistant microvines were obtained. The MAS results were also validated by leaf bioassays, which confirmed that resistance genes segregated to the F1 progeny are functional and provide resistance. To the best of our knowledge, this research provides the first photographic validation of the powdery and downy mildew resistance of the Turkish variety ‘Bogazkere’. Our findings could potentially provide the wine industry with a resistant wine grape cultivar and an addition to the wine grape germplasm, which could contribute to future breeding programs. Most importantly, the success of the resistance genes segregated into the candidate progeny could help significantly reduce the use of chemicals against grapevine pathogens.

  • Research Article
  • Cite Count Icon 1
  • 10.61475/jfm.v36i02.9
Effect of different temperatures, relative humidity, incubation period on conidialgermination of Erysiphe necator causing powdery mildew of grapes
  • Jun 25, 2023
  • Journal of Farm Sciences
  • Sagar N + 4 more

In vitro studies were conducted to assess the effect of different temperatures, relative humidity and incubationperiod on conidial germination of Erysiphe necator (powdery mildew) at the Department of Plant Pathology, College ofAgriculture, Vijayapur during 2020-22. Physiological studies of E. necator revealed that incubation of powdery mildewfungal spore suspension at various intervals indicated fairly satisfactory conidial germination from 18 to 22 hrs andultimately reached maximum at 24 hrs after incubation in distilled water. Among the different relative humidity (RH) levelsused for conidial germination, maximum germination was observed at 80 per cent RH (82.67%). howver, relative humidityranging from 80 to 85 per cent was found suitable for conidial germination of E. necator. Maximum conidial germination(83.69%) of E. necator was observed at 25 °C after 24 hrs and optimum temperature range for the conidial germination was20-25 °C.

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  • Research Article
  • 10.15835/buasvmcn-hort:2023.0002
The Influence of Climatic Conditions on Downy Mildew and Powdery Mildew in Târnave Vineyard
  • Jun 21, 2023
  • Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture
  • Maria Comșa + 7 more

The main diseases that affect the vineyards in the Târnave vineyard are: grapevine downy mildew caused by Plasmopara viticola and grapevine powdery mildew caused by Uncinula necator. The present study aimed to address the relationship between grapevine cultivars and the main diseases, downy mildew and powdery mildew, according to climatic variability and treatments applied. The analysis was carried out over two consecutive years 2021 and 2022 on five new cultivars, the creation of SCDVV Blaj: Selena, Blasius, Rubin, Radames and Brumăriu. Disease attack was determined during the vegetation period, until harvest. Cultivar susceptibility varied, some cultivars were relatively tolerant and no cultivar was highly resistant to both diseases. In addition, a difference between foliar and grape berry susceptibility to the two diseases was observed for several cultivars. This data provide a basis for developing low-treatment disease management strategies for specific grapevine cultivars based on downy mildew and powdery mildew susceptibility/tolerance.

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  • Cite Count Icon 1
  • 10.30901/2658-6266-2022-4-o1
&lt;i&gt;In vitro&lt;/i&gt; regeneration of grape
  • Apr 5, 2023
  • Plant Biotechnology and Breeding
  • T. V. Kovalenko + 3 more

Considering the global and Russian experience in grape accessions preservation, one of the most reliable ways is the creation of a duplicate in vitro collection. However, in connection with the creation of duplicate grape collections and development of genome editing techniques, there is a need for selecting the most optimal medium composition that will ensure the maximum rate of callus formation and regeneration during the introduction of grape plants into in vitro culture. This will make it possible to obtain the necessary amount of material for further editing and subsequent regeneration of plants with knockout of target genes to improve economically valuable traits. For grapes, this is primarily an increase in resistance to powdery mildew caused by Uncinula necator Burill.The effect of active substances of biological and synthetic origin on the morphogenesis has been studied for many crops, including regenerant plants of the genus Vitis L. However, the genus Vitis is very diverse and heterogeneous in its genetic, physiological and morphological structure, as a result of which the recommended media and components for cultivation under in vitro conditions may not suit every cultivar. Local Russian grape cultivars are better suited to local growing conditions, so efforts should be focused on the development of techniques related to the preservation of local varieties in collections in vitro.Knowledge of genes controlling certain traits, as well as the availability of grape accessions whose genome has been sequenced, contribute to successful in silico analysis for creating editing constructs.

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  • Cite Count Icon 1
  • 10.59904/gi.v1.i1.2023.9
Compatibility of &lt;i&gt;Trichoderma asperelloides&lt;/i&gt; with Fungicides Controlling Downy Mildew and Powdery Mildew in Grapes
  • Jan 16, 2023
  • Grape Insight
  • S Saha + 3 more

The grape (Vitis vinifera) is an important horticultural crop which belongs to the family Vitaceae. Downy mildew (casual organism: Plasmopara viticola) and powdery mildew (casual organism: Uncinula necator) are two major diseases of grapevine which limit the production of grapes significantly. Both fungicides and bio control agents are used to manage diseases, but both have their own limitations. Integration of bio control agents along with fungicides in disease management helps in reduction of fungicide usage. To understand the effect of these fungicides with commonly used bio-control agents, a compatibility study was carried out under in-vitro condition at ICAR-NRC for Grapes, Pune. Registered fungicides against downy mildew and powdery mildew viz., Mancozeb 75% WP, Dimethomorph 50% WP, Fosetyl Al 80% WP, Amisulbrom 17.7% SC w/w (20% SC w/v), Copper oxychloride 50% WP, Kresoxim methyl 44.3% SC, Tetraconazole 3.8% EW, Metrafenone 50% SC, Cyflufenamid 5% EW and Azoxystrobin 11% + Tebuconazole 18.3% w/w were evaluated for compatibility with a potent bio-control agent viz. Trichoderma asperelloides. Trichoderma asperelloides was found to be fully compatible with Fosetyl Al 80% WP, Amisulbrom 17.7% SC w/w (20% SC w/v) and Cyflufenamid 5% EW and to some extent with Mancozeb 75% WP, Dimethomorph 50% WP at their recommended doses. Such compatibility studies help in understanding the gap between the application of synthetic chemicals and bio control agents.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 2
  • 10.1051/e3sconf/202338903050
Monitoring of disease and pests in vineyards and their levels of harmfulness
  • Jan 1, 2023
  • E3S Web of Conferences
  • Asror Rakhmatov + 7 more

This article monitors the spread and damage of oidium (Uncinula necator Burrill), anthracnose (Gloeosporium ampelophagum Sacc) and mildew (Plasmopara viticola Berl &amp; De Toni) diseases in the vineyards of Tashkent, Samarkand and Fergana regions of Uzbekistan. In addition, additional monitoring work was carried out on the spread of the external quarantine vine bacteriosis Pierce's disease (Xylella fastidiosa Wells et al) in the vineyards of Uzbekistan. According to the results of research and observation in 2022, vine oidium disease is relatively low in the vineyards of Samarkand and Fergana regions (from 5.0% to 30.0% in leaves, branches and grape bunches), and strongly in the vineyards of Tashkent region (12% in leaves, branches and grape bunches). .0% to 100.0%) have been found to be harmful. Anthracnose disease is widespread in the vineyards of Tashkent and Samarkand regions (from 10.0% to 75.0% on leaves, branches and bunches of grapes), causing severe damage to vineyards, and it is relatively rare in Fergana region (from 5.0% to 20.0% on leaves, branches and bunches of grapes) was found to be spread.

  • Open Access Icon
  • Research Article
  • 10.29329/ijiaar.2022.506.9
Technological Characteristic of Cabernet Sauvignon Wine Produced from Organically Grown Grapes
  • Dec 31, 2022
  • International Journal of Innovative Approaches in Agricultural Research
  • Tatyana Yoncheva

The opportunities for organic growing of the Cabernet Sauvignon variety in the region of the town of Pleven, Central Northern Bulgaria, were studied during the period 2017-2020. Conventional and organic plant protection measures against the diseases downy mildew (Plasmopara viticola) and powdery mildew (Oidium tuckeri) were undertaken in the experimental plantations. In the organic cultivation of the variety, the products Funguran OH 50 WP and Thiovit Jet 80 WG were used, with seven treatments carried out annually. In the conventional cultivation, five or six combined treatments were applied. In the period of technological maturity, a minor attack of downy mildew and powdery mildew was found on the foliage and the clusters in both variants. The damage index from both diseases was higher in the organic production, but the differences found did not have a significant impact on the quality of the grape harvest. In the organic grape-growing, the amount of the input elemental Cu was strictly monitored. A technological characteristic of the Cabernet Sauvignon wines obtained from both growing variants was made. The difference in the main indicators of the grapes chemical composition was insignificant. The average rate of sugars, total acidity and glucoacidimetric index was similar. No significant differences were observed in the ratios of most indicators of the wines’ chemical composition. The contents of alcohol, sugars, total acidity and extract in the samples of both variants were identical. More differences stood out relating the total phenolic compounds, anthocyanins and colour intensity, as their rates were higher in the conventional wines. Due to their better taste and color features, these samples had higher tasting scores and were superior in their organoleptic qualities compared to the organic wines.

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