Ultraviolet-visible Spectroscopy Research Articles

Green synthesis, characterization, antioxidant, anti-fungal, antibacterial activities of a new 1-isopropyl-3,5-di-2-tolyl-1,3,5-triazacyclohexane

This work presents the green synthesis of a novel of 1- isopropyl- 3, 5- di-2-tolyl- 1, 3, 5-triazacyclohexane in good yield. It is made without a solvent, energy, and a catalyst as a green technique via a one-pot multi component condensation reaction between one equivalent of isopropylamine, two equivalents of o-toluidine, and three equivalents of formaline. The compound's structure has been determined using several spectroscopic methods, including thin-layer chromatography (TLC), ultraviolet-visible spectroscopy (UV-Vis), infrared spectroscopy (IR), and nuclear magnetic resonance spectroscopy (NMR) for both 1H and 13C nuclei. Additionally, the compound 'smelting point was determined. Subsequently, the molecule was evaluated for its antioxidant and biological activities. The antioxidant activity exhibited good performance in both the ABTS and Phenanthrolinetests. The antimicrobial activity of this novel compound was tested against four bacterialstrains, including two Gram-positive bacteria (Staphylococcus aurous ATCC 25923 and Bacillus cereus ATCC 10876) and two Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853), as well as two fungal strains (Fusarium oxysporum and Alternariaalternata)using the agar diffusion method and the direct contact method, respectively. The obtained results exhibited a high level of antibacterial and antifungal activity.

PH-Sensitive and Redox-Responsive Poly(Tetraethylene Glycol) Nanoparticle-Based Platform for Cancer Treatment.

Effective drug delivery with precise tumour targeting is crucial for cancer treatment. To address the challenges posed by the specificity and complexity of the tumour microenvironment, we developed a poly(tetraethylene glycol)-based disulfide nanoparticle platform and explored its potential in cancer treatment, focusing on drug loading and controlled release performance. Poly(tetraethylene glycol) nanoparticles were characterised using nuclear magnetic resonance spectroscopy, mass spectrometry, and ultraviolet-visible spectroscopy. Additionally, we evaluated physicochemical properties, including dynamic light scattering, zeta potential analysis, drug loading capacity, and drug loading efficiency. The impact of nanoparticles on the mouse colorectal cancer cell line (CT26) and NIH3T3 cells was assessed using a cytotoxicity assay, live/dead staining assay, flow cytometry, and confocal fluorescence microscopy. The experimental results align with the expected chemical structure and physicochemical properties of poly(tetraethylene glycol) nanoparticles. These nanoparticles exhibit high drug loading efficiency (78.7%) and drug loading capacity (12%), with minimal changes in particle size over time in different media. In vitro experiments revealed that the nanoparticles can induce significant cytotoxicity and apoptosis in CT26 cells. Cellular uptake notably increases with increasing concentration and exposure time. The confocal microscopic analysis confirmed the effective distribution and accumulation of nanoparticles within cells. In conclusion, poly(tetraethylene glycol) nanoparticles hold promise for improving drug-delivery efficiency, offering potential advancements in cancer treatment.

Construction of novel nanocomposite based on the reduced graphene oxide/metalation of porphyrin for photocatalytic process by real sample analysis and antimicrobial applications

Abstract The main objective of this study is to prepare and combine reduced graphene oxide (rGO) with copper-5,15-bisdodecylporphyrin (Cu-Por) nanoparticles in order to produce a unique rGO-loaded Cu-Por nanocomposite. Characterization techniques such as scanning electron microscopy (SEM), Raman spectroscopy, X-ray diffraction (XRD), X-ray photoelectron absorption spectroscopy (XPS) and ultraviolet-visible (UV-Vis) spectroscopy were utilized to analyze the structure of the synthesized rGO/Cu-Por. The effectiveness of the resulting rGO/Cu-Por nanocomposite as a catalyst was evaluated based on several parameters, such as pH, the initial concentration of the MR dye, and the amount of nanocomposite employed. Remarkably, a quantity of 20 mg of the rGO/Cu-Por nanocomposite demonstrated a significant removal efficiency of 99.7% for MR at a pH level of 3.5. The actual sample exhibited a degradation amount of 69.21%. In addition, the antimicrobial efficacy of the nanocomposite was assessed against the G-negative and the G-positive bacterium. The findings demonstrate that the rGO/Cu-Por nanocomposite displayed significant antimicrobial activity with great zone of inhibition values.

Green synthesis of silver and gold-doped zinc oxide nanocomposite with propolis extract for enhanced anticancer activity

Metal and metal oxide nanocomposites have unique properties and are promising for antibacterial and anticancer applications. In this work, we aimed to highlight the relationship between the biosynthesis ways of silver and gold-doped zinc oxide nanocomposites and their functions as anticancer on cell lines (MCF-7 and HepG2). The propolis was used to biosynthesize four different nanoparticles with the same components, including zinc, gold and silver. The nanocomposites were characterized using various techniques, including ultraviolet–visible spectroscopy (UV–Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Energy Dispersive X-ray analysis (EDX) and cytotoxicity assays. The result of this study showed that formed nanocomposites have a similar level of Zn, Au, and Ag, ranging from 23–34%, 2–6%, and 2–3%, respectively. In addition, adding the components simultaneously produces the fastest color change, and the fabricated nanoparticles have spherical shapes with different layers. In addition, the prepared nanoparticles influenced the cell viability of the cancer cell lines, with the most effective one when Zn, Au, and Ag were added spontaneously to form a nanocomposite called (All) with IC50 of 24.5 µg/mL for MCF7 cells and 29.1 µg/mL for HepG2 cells. Thus, the study illustrates that the preparation of nanocomposite generated through green synthesis with different methods significantly affects the structure and function and may improve the synthesis of nanocomposite to be developed into an efficacious therapeutic agent for cancers. In addition, this study opens the door toward a novel track in the field of nanocomposites as it links the synthesis with structure and function. Further anti-cancer properties, as well as animal testing are needed for those nanocomposites.

Two-Dimensional Correlation Spectroscopy (2D-COS) Tracking of the Formation of Selected Transition Metal Compounds Cu(II) and Cd(II) With Cinchonine and Their Impact on Model Components of Erythrocytes.

Cinchonine is a quinoline alkaloid known for its antimalarial properties. Due to the advantages of using compounds of metal ions with alkaloids, a copper(II) compound with cinchonine was synthesized, and, for comparative purposes, a cadmium(II) compound with cinchonine. During the synthesis, the emerging interactions between the metal ion and cinchonine were studied. After crystallization, it was examined how the obtained compounds would interact with the model blood component, hematoporphyrin IX. Ultraviolet-visible (UV-Vis) spectroscopy, Raman spectroscopy, and attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) were used in the study. In the case of monitoring the synthesis, the best method turned out to be UV-Vis spectroscopy, combined with the possibility of two-dimensional correlation spectroscopy (2D-COS), which enabled the identification of peaks characteristic of the interactions of the cinchonine quinoline ring with metal ions. In turn, the obtained Raman spectra showed shifts of individual bands and changes in their intensity, and 2D-COS showed the sequence of formation of individual interactions, which confirmed the formation of cinchonine compounds with metals. ATR FT-IR also allowed us to compare the spectra of the substrates used in the synthesis with the crystallized compounds and thus confirm the formation of the expected compounds. Bands characteristic of π-π-stacking interactions between the quinoline ring and the tetrapyrrole ring of hematoporphyrin IX were also observed. Observed interaction with a model blood component may be important when designing drugs for antimalarial therapy.

Using the Plasma Jet Method to Create Zinc Nanoparticles and Study Their Structural and Optical Properties as an NH3 Gas Sensor

In this work, zinc nanoparticles were prepared using the low-temperature plasma method at different times 3 min, 5 min, 7 min, 9 min, where the initial reactions of zinc lead to the formation of nanoparticles. The metal interacts with the ionized plasma under different conditions and may turn into an oxide such as exposing the sample to air and raising the temperature of the solution a few degrees as a result of high voltage and within a certain range. The atmospheric pressure plasma system acts as the cathode, while the zinc metal strip acts as the anode. A series of techniques were used, including the use of X-ray diffraction (XRD), ultraviolet-visible spectroscopy and scanning electron microscopy (FESEM). The X-ray diffraction results showed that the samples have polycrystalline structures with hexagonal and cubic structures with a time difference, and the X-ray diffraction results showed the conversion of zinc particles into zinc oxides. FESEM images reveal particle size and shape. The ZnO nanoparticles prepared by the mentioned method seem to form different nanoshapes such as starfish and mushroom nanoparticles. The UV-visible results showed that the samples had an energy gap of about 3.4 eV at a time of 3 min, and this value decreases with increasing reaction time. The appearance of zinc oxides increases gradually with the passage of time. These results provide credibility for the fabrication of ZnO nanostructures for use in future gas sensing applications, despite the inhomogeneity of particle size among ZnO particles, the sensor was also fabricated to detect nitrogen gas (NH3) with different concentrations (17.25 ppm, 46.38 ppm, 78.58 ppm). at room temperature. The ZnO sample showed the highest sensitivity (88.1%) at 7 min. The sensitivity showed different results and increased with reaction time and gas concentration. However, response and recovery time are moderate and decrease as reaction time increases.

Pd Nanoparticle-Modified BiOBr/CdS S-Scheme Photocatalyst for Enhanced Conversion of CO2.

S-scheme heterojunction photocatalyst-coupled plasma-resonance effect can enhance the response range and absorption of light and charge transfer, and, at the same time, obtain strong redox ability, which is an effective way to improve CO2 conversion. In this work, plasma S-scheme heterojunctions of Pd/BiOBr/CdS with heterogeneous interfaces have been successfully constructed by a simple hydrothermal method. The possible reaction mechanism was proposed by in situ infrared, ultraviolet-visible spectroscopy (UV-vis), electron paramagnetic resonance (ESR), density functional theory (DFT), and electrochemical techniques. It was proved that the plasma S-scheme heterojunction can enhance the charge separation efficiency and improve the photocatalytic activity. When the loading ratio is Pd0.6-10%-BiOBr/CdS, it has the best performance, and the CO yield is 30.24 μmol/g, which is 15 and 30 times that of pure BiOBr and CdS, respectively. The results show that with the strong absorption of photon energy and the special electron transfer mode of S-scheme heterojunction, the charge can be effectively separated and transferred, and the photocatalytic activity is significantly improved. This study provides a useful strategy for charge transfer kinetics of plasma S-scheme heterojunction photocatalysts.

A smartphone sensing fluorescent detection of mercury ion based on silicon quantum dots in environment water

Mercury ion (Hg2+) pose a significant hazard to the natural environment. Conventional techniques like Inductively coupled plasma mass spectrometry, X-ray absorption spectroscopy, among others, pose some disadvantages as they demand a lot of money, need trained employees, and cannot provide on-site detection in real-time. A smartphone sensing technique based on silicon quantum dots (Si-QDs) was presented to detect Hg2+ in the environment without the usage of sophisticated equipment. Meanwhile, the technology was built by utilizing a smartphone to capture gray values of fluorescent images of the Si-QDs-Hg2+ system. Microwave-assisted Si-QDs with tiny particle size, high fluorescence, and good optical stability were created. The fluorescence of the Si-QDs was gradually quenched by raising the Hg2+ concentration from 0.5 μmol/L to 5.0 μmol/L for fluorescent detection with a detection limit of 28 nmol/L. The 94.8–97.1 % recovery demonstrated the viability of the Si-QDs approach for detecting Hg2+. Meanwhile, a smartphone sensing strategy was built by recording the gray value of the fluorescent images of the Si-QDs-Hg2+ systems using a smartphone, and the detection limit of the established approach was 3 nmol/L. The accuracy and reliability of the smartphone strategy were verified with the recovery rates of 80.3–92.5 % in tap water and 87.6–109 % in river water. Electron transfer quenching mechanism between Si-QDs and Hg2+ was evidenced by ultraviolet–visible spectroscopy, fluorescent decay curves, cyclic voltammetry, and Zeta potential. Finally, the suggested approach was used to detect Hg2+ in water samples from various environments.

A Machine Learning Approach for Efficiently Predicting Polymer Aging from UV-Vis Spectra.

This research has introduced an innovative approach that proficiently forecasts the alterations in ultraviolet-visible spectroscopy (UV-Vis) of polymer solutions during the aging effect. This method combines readily accessible feature descriptors with classical machine learning (ML) algorithms. Traditional spectral measurements, while precise in analyzing physical properties, are limited by their cost and efficiency. Therefore, this paper introduces a method that utilizes wavelength and the blue (B), green (G), and red (R) color values of the solutions as input features. We employed seven different ML models to train on these features with 10-fold cross-validation to ensure the reliability and generalizability of our results. After comparative analysis, all of the models performed excellently. Among them, the ExtraTree model demonstrated particularly high precision and excellent predictive ability on the testing set, with a Pearson correlation coefficient (r) of 0.9859 and a mean absolute error (MAE) of 0.0457. This study offers a practical solution for the rapid and cost-effective evaluation of polymer solutions' aging effect.

Solar Light CO2 Photoreduction Enhancement by Mononuclear Rhenium(I) Complexes: Characterization and Mechanistic Insights.

The catalytic efficacy of a novel mononuclear rhenium(I) complex in CO2 reduction is remarkable, with a turnover number (TONCO) of 1517 in 3 h, significantly outperforming previous Re(I) catalysts. This complex, synthesized via a substitution reaction on an aromatic ring to form a bromo-bipyridine derivative, L1 = 2-bromo-6-(1H-pyrazol-1-yl)pyridine, and further reacting with [Re(CO)5Cl], results in the facial-tricarbonyl complex [ReL1(CO)3Cl] (1). The light green solid was obtained with an 80% yield and thoroughly characterized using cyclic voltammetry, nuclear magnetic resonance (NMR), Fourier transform infrared (FTIR) spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy. Cyclic voltammetry under CO2 atmosphere revealed three distinct redox processes, suggesting the formation of new electroactive compounds. The studies on photoreduction highlighted the ability of the catalyst to reduce CO2, while NMR, FTIR, and electrospray ionization (ESI) mass spectrometry provided insights into the mechanism, revealing the formation of solvent-coordinated complexes and new species under varying conditions. Additionally, computational studies (DFT) were undertaken to better understand the electronic structure and reactivity patterns of 1, focusing on the role of the ligand, the spectroscopic features, and the redox behavior. This comprehensive approach provides insights into the intricate dynamics of CO2 photoreduction, showcasing the potential of Re(I) complexes in catalysis.

Biomedical applications of chitosan-coated phytogenic silver nanoparticles: An alternative drug to foodborne pathogens

The biogenic synthesis of silver nanoparticles (AgNPs) was performed using crude rosmarinic acid (RA) from plants as a reducing agent and coated with chitosan biopolymer. The prepared particles were characterized by ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). A surface plasmon resonance peak at 430 nm indicates the emergence of AgNPs. XRD showed that the AgNPs were crystalline with an average crystalline size of 30 nm and TEM studies revelad that AgNPs were spherical without aggregation. The prepared CS-AgNPs exhibited good bactericidal properties against foodborne pathogens, such as Escherichia coli, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. In particular, 100 μg/mL CS-AgNPs inhibited the growth of the selected bacteria and controlled their biofilm-forming ability. Band-aid cloth assay confirmed that the CS-AgNPs could be used in the medical field to prevent bacterial infections. The prepared CS-AgNPs increased the survival rate of Artemia species and exhibited antioxidant activity in conjunction with bactericidal properties against selected foodborne pathogens.

Novel biopolymeric chitosan-urea-based core-shell hydrogel for use in sustainable agriculture

Agricultural sustainability is a major issue in recent times, deficiency of water as well as insufficient fertilizer to the crop is a main reason for it. This work is focused on core-shell hydrogel aiming to overcome the agricultural issues of water as well as deficiency of fertilizer to the plant. The core-shell hydrogel was prepared by incorporating urea in κ-carrageenan to be part of the controlled release mechanism of the core of the hydrogel. Chitosan was used for the shell structure to prepare over the core and cross-linked by the cations of the salts. The prepared hydrogel was termed as Chitosan-κ-carrageenan-Urea (CK-CU). Four different types of hydrogels were prepared with the different compositions named as CK-CU 2, CK-CU 5, CK-CU 7.5 and CK-CU 10 as varying with the ratio of 2 %, 5 %, 7.5 % and 10 % of urea respectively. The core-shell morphology structure was verified with Scanning electron microscopy (SEM) in which the complete coverage of the κ-carrageenan core by a consistent chitosan shell has been verified. Fourier transform infrared spectroscopy (FTIR) was utilized to confirm the composition of the hydrogel. Thermo-gravimetric analysis (TGA) was also performed for checking the thermal endurance of the hydrogel. The release rate of the urea was determined under Ultraviolet Visible spectroscopy. The swelling index, dewatering, and urea release rate of optimized CK-CU 10 hydrogel were found to 121 %, 21.22 %, and 70.4 % respectively. The developed Core-Shell hydrogel exhibits significant promise as a vehicle for fertilizer and as a soil conditioner in agricultural applications especially in arid and semi-arid areas.

Assessment of acid catalytic properties of ferrosilicate MFI zeolite by methanol-to-hydrocarbon conversion.

Four representative synthetic methods were employed to prepare Fe-containing siliceous MFI zeolites. The obtained Fe-MFI zeolites exhibited markedly different catalytic performances in the methanol-to-hydrocarbon (MTH) conversion reaction depending on the type of Fe incorporation within the siliceous framework. The catalytically active Brønsted acid sites were analyzed using pyridine adsorption experiments combined with Fourier transform infrared spectroscopy, providing characteristic signal intensities according to the acid-base interactions. Based on the MTH conversion results and acidity analyses, a suitable synthetic method was identified for the incorporation of Fe within the MFI zeolite framework. However, compared to other catalytic reactions, structural analyses by transmission electron microscopy, ultraviolet-visible spectroscopy, and X-ray absorption spectroscopy were much less conclusive.

Novel α-N-heterocyclic thiosemicarbazone complexes: synthesis, characterization, and antimicrobial of properties investigation.

In this paper, eight novel α-N-heterocyclic thiosemicarbazone complexes were synthesized in search of new biologically active compounds, and characterized via organic elemental analysis, nuclear magnetic resonance spectroscopy, infrared spectra, thermogravimetric analysis, ultraviolet-visible spectroscopy, molar conductance and magnetic susceptibility measurements. The in vitro antimicrobial activity of these complexes was examined against ten disease-causing pathogens: Gram-positive bacteria (Micrococcus luteus ATCC9341, Staphylococcus epidermidis ATCC12228, Bacillus cereus RSKK863) and Gram-negative bacteria (Pseudomonas aeroginosa ATCC27853, Klebsiella pneumonia ATCC27853, Enterobacter aerogenes ATCC51342, Salmonella typhi H NCTC9018394, Shigella dysenteria NCTC2966, Proteus vulgaris RSKK96026) and yeast (Candida albicans Y-1200-NIH). The results revealed that the α-N-heterocyclic thiosemicarbazone compounds showed potent activity. It was observed that all thiosemicarbazone complexes were more susceptible to Gram-negative strains based on the presence of an electron-withdrawing substituent (-Br/-Cl/-F). It was determined that thiosemicarbazone Cu2+complexes showed stronger antifungal effects.

Diffusion-Regulated Interfacial Polymerization of Hierarchically Microporous Polyamide Membranes for Permselective Gas Separations.

Interfacial polymerization has emerged as a robust method for fabricating task-specific polyamide (PA) membranes. However, the limited microporosity of highly cross-linked PA membranes constrains their effectiveness in gas separation applications. Herein, we introduce an ionic liquid (IL)-regulated interfacial polymerization process to fabricate polyamide nanofilms incorporating kinked tetrakis (4-aminophenyl) methane monomers. In situ ultraviolet-visible spectroscopy demonstrates that the diffusion of 1,3,5-benzenetricarbonyl trichloride (TMC) toward the interface increases with the IL/H2O ratio, leading to the formation of a more compact membrane with a higher cross-linking degree. The PA-TAM7/3-60 min membrane exhibits a CO2 permeance of 29.8 GPU and a CO2/CH4 selectivity of 109, exceeding the 2008 Robeson upper bond. Additionally, the highly cross-linked structure imparts the membranes with notable plasticization resistance. Mixed-gas tests (CO2/CH4 = 50/50, v/v) reveal that the PA-TAM7/3-60 min membrane experiences only a 2% reduction in CO2 permeance and a 10% decrease in CO2/CH4 selectivity at a CO2 partial pressure of 300 PSIG, compared to its performance at 30 PSIG. The ease of tuning membrane structure and gas separation performance, along with its excellent plasticization resistance, underscores the potential of these PA membranes for task-specific gas separations.

A Self-Skin Permeable Doxorubicin Loaded Nanogel Composite as a Transdermal Device for Breast Cancer Therapy.

Modern drug delivery research focuses on developing biodegradable nanopolymer systems. The present study proposed a polymer-based composite nanogel as a transdermal drug delivery system for the pH-responsive targeted and controlled delivery of anticancer drug doxorubicin (DOX). Nanogels have properties of both hydrogels and nanomaterials. The β-cyclodextrin-based nanogels can enhance the loading capacity of poorly soluble drugs and promote a sustained drug release. The β-cyclodextrin-grafted methacrylic acid conjugated hyaluronic acid composite nanogel was successfully synthesized. β-Cyclodextrin was first grafted onto methacrylic acid. The composite nanogel-based drug carrier was prepared by controlled radical polymerization (CRP) of β-cyclodextrin-grafted methacrylic acid with hyaluronic acid. The doxorubicin-loaded carrier was characterized by Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, ultraviolet-visible (UV-vis) spectroscopy, zeta potential analysis, dynamic light scattering (DLS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The drug loading and release efficiencies were carried out at different pH levels. The maximum drug loading and encapsulation efficiencies of the synthesized final nanogel composite material at pH 8.0 were 86.44 ± 2.12 and 96.07 ± 2.01%, respectively. The DOX-loaded final material showed a 90.0 ± 2.6% release percentage of DOX at pH 5.5, whereas at pH 7.4, the release percentage of DOX was observed to be only 35.0 ± 0.3%. In vitro swelling, degradation, hemocompatibility, drug release kinetics, cytotoxicity, apoptosis, cell colocalization, skin irritation, and skin permeation studies, along with in vivo pharmacokinetic studies, were performed to prove the efficacy of the synthesized nanogel composite as a transdermal carrier for doxorubicin.

The Study of Calcium Chloride Effect on Silver Nanoparticles Capping with Roselle Extract Granule against Aggregatibacter actinomycetemcomitans

Abstract Objectives The primary aim of this research is to investigate the influence of calcium chloride on the synthesis of silver nanoparticles coated with roselle extract and enclosed within alginate and calcium chloride (SNP-Ro-CaCl2) beads, designated as SNP-Ro-CaCl2 beads. Additionally, the study aims to assess their antimicrobial activity. Materials and Methods For the preparation of SNP-Ro-CaCl2 beads, SNPs and alginate gel were mixed, followed by dropping in three different concentrations of CaCl2 solution (1%, 3%, and 5% w/v). The morphological structure of the SNP-Ro-CaCl2 beads was analyzed using a stereoscope and scanning electron microscope (SEM). Over a period of 14 days, the release of SNPs was monitored using ultraviolet-visible (UV-Vis) spectroscopy. Additionally, the activity against Aggregatibacter actinomycetemcomitans was evaluated using the disk diffusion technique. Statistical Analysis The data for this experiment were analyzed using one-way analysis of variance (ANOVA) and Scheffe's method. Results The results revealed that varying concentrations of calcium chloride had distinct crosslinking effects on alginate, resulting in different voids and porosity within the SNP-Ro-CaCl2 beads. In the SNP-Ro-1% CaCl2 beads, the inner element exhibited higher porosity, facilitating faster activation and greater efficiency in releasing SNPs. Regarding activity against A. actinomycetemcomitans after 14 days, SNP-Ro-1% CaCl2 beads showed a larger inhibition zone diameter compared to other concentrations, while no statistically significant difference in the inhibition zone diameter was observed between SNP-Ro-3% CaCl2 and SNP-Ro-5% CaCl2 beads. Additionally, it was observed that the antimicrobial effectiveness diminished after 17 days through testing of the lifetimes of the three concentrations. Conclusions This study developed a method for depositing SNP-Ro into alginate gel and crosslinking it with CaCl2 to produce small beads for the sustained release of SNP-Ro in periodontal lesions. Consequently, the SNP-Ro-CaCl2 beads have the potential to be developed as adjunctive locally delivered antimicrobial agents in periodontal therapy.

Biogenic Syntheses of AgNPs With Leaf Extract Abution indicum and Its Application for Antimicrobial and Photocatalytic Activity

ABSTRACTThe biogenic synthesis of silver nanoparticles (AgNPs) with leaf extract Abution indicum was done, and those were characterized by ultraviolet–visible spectroscopy (UV–vis), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), powder X‐ray diffraction (PXRD), field emission scanning electron microscopy (FESEM) and transmission electron microscope (TEM) with energy‐dispersive X‐ray (EDX) spectroscopy. The analysis by UV–vis spectroscopy showed a peak of 450 nm, and DLS and zeta potential were utilized to determine the size distribution of the biosynthesized Abution indicum–AgNPs (AI‐AgNPs) with a size range of 24–37 nm, and the X‐ray diffraction peak 38.096o was used to confirm that the crystalline structure of AI‐AgNPs. Furthermore, the antipathogenic effect of synthesized AgNPs and standard antibiotic (Ciprofloxacin) as studied the positive control in different types of bacterial pathogens likes Staphylococcus aureus and Escherichia Coli, with the zone of inhibition values of 9 mm. The synthesized AgNPs displayed excellent photocatalytic activity against reactive blue under sunlight than a UV light irradiation, and maximum degradation of 43% was achieved with 66 min of reaction time. In view of promising activity, the AgNPs could be used photocatalyst for the degradation of dyes in wastewater, and pomegranate leaf extract can be applied as eco‐benign and cost‐effective approach for AgNPs synthesis. Hence, the current findings suggest that Abutilon indicum is a valuable source for tailoring the potential of AgNPs toward various enhanced biological, photocatalytic, and adsorption activities. Consequently, the plant biological molecule‐mediated synthesized AI‐AgNPs could be excellent contenders for future therapeutic applications.

A Facile and Green Approach for the Preparation of Silver Nanoparticles on Graphene Oxide with Favorable Antibacterial Activity.

Herein, we introduce a simple precipitation method for preparing graphene oxide-silver nanoparticle (GO/AgNP) composites, utilizing Calendula officinalis (C. officinalis) seed extract as both a reducing and stabilizing agent. Our research combines the sustainable preparation of graphene oxide (GO) with the green synthesis of silver nanoparticles (AgNPs), aiming to explore the potential of the obtained composite as a novel antibacterial material. To establish a benchmark, the synthesis was also performed using sodium citrate, a conventional reducing agent. The resultant GO/AgNP composites were characterized through several analytical techniques, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDS), Raman spectroscopy, X-ray diffraction (XRD), infrared (IR) spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy, confirming the successful functionalization of GO with AgNPs. The antibacterial effectiveness of the composites was systematically assessed against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with nanoparticle concentrations spanning from 0 to 250 µg/mL, utilizing mostly disk diffusion and colony-forming unit (CFU) count assays. The AgNPs were characterized by a size range of 15-50 nm. Notably, the GO/AgNP composite prepared using C. officinalis seed extract demonstrated superior antibacterial activity at all tested concentrations, outperforming both pure GO and the GO/AgNP composite prepared with sodium citrate. The most pronounced antibacterial effect was observed at a concentration of 32.0 µg/mL. Therefore, this innovative synthesis approach may offer a valuable contribution to the development of new therapeutic agents to combat bacterial infections, suggesting further exploration into antibacterial coatings or potential drug development.

Characterization of Mung Plants Treated with Iron Oxide Nanoparticles Using Raman and Ultraviolet-Visible Spectroscopy Coupled with Chemometrics

In order to meet the demands of the expanding population, nanotechnology-based materials are being investigated for a potential solution to enhance crop yield. This study examines the effect of different concentrations (0.2, 0.6, 1.0, and 1.4 mM) of iron oxide nanoparticles (Fe2O3 NPs) on mung plants using confocal micro Raman and ultraviolet-visible spectroscopy. The analysis shows that the biochemical profile of the plants is altered. The increased intensity values of carotenoids, pectin, lignin, proteins, cellulose, carbohydrates, and aliphatics observed in Raman spectroscopy, along with increased concentrations of photosynthetic pigments in ultraviolet-visible spectroscopy, suggest that Fe2O3 NPs positively impact mung. In agro-related studies, Raman spectroscopy in assistance with chemometric analysis provides a reliable, noninvasive, fast, and label-free recognition and classification of plants under biotic or abiotic stress. Principal component analysis (PCA), linear discriminant analysis (LDA), and principal component analysis-linear discriminant analysis (PCA-LDA) were applied to the Raman spectra of treated and untreated mung leaves for rapid categorization and precise prediction. The results show that PCA-LDA achieved superior predicting performance and visualization, with an accuracy of 70%, along with higher recall, precision, f1 score, and area under receiver operating characteristics curves compared to LDA. The study reveals that spectroscopic techniques combined with chemometrics have great potential for confirmatory, nondestructive, and label-free assessments of biotic and abiotic stresses in plants.