Two-channel Detection Research Articles

A multifunctional electrochemiluminescence and photoelectrochemical biosensor based on a quantum dot ion-exchange reaction for two-channel detection of thrombin.

Herein, a multifunctional electrochemiluminescence (ECL) and photoelectrochemical (PEC) biosensor based on exchange of Ag+ with CdTe QDs was developed for dual-mode detection of thrombin. First, CdTe QDs assembled on an electrode displayed superior ECL and PEC signals. At the same time, C-rich hairpin (HP) DNA linked to silicon spheres loaded a large amount of Ag+, and the specific binding of thrombin to an aptamer led to the release of DNA P; then, DNA P interacted with HP DNA to produce numerous Ag+ ions by an enzyme-digestion amplification reaction. Ag+ underwent ion exchange with CdTe QDs to generate AgTe/CdTe QDs, resulting in much reversed PEC and changed ECL signals for dual-mode detection of thrombin. This work takes advantage of outstanding multi-signals of QDs coupled with convenient ion exchange to achieve multi-mode detection of the target, avoiding false positive or false negative signals generated in the traditional detection process, and thus can be used for the rapid detection of various biomolecules in actual samples.

Optical image encryption based on two-channel detection and deep learning

Aside from its application to the vulnerability analysis of optical cryptosystems, deep learning (DL) technique can also be used as a potential basis for optical cryptography itself. Here, we report a new optical image encryption scheme based on two-channel detection and DL. The encryption process consists of two-channel signal detection within the optical encryption system where three random binary masks are involved, and the training of a neural network to derive an approximate model. The two optically encoded patterns collected with a pair of CCD cameras are recombined into a single speckle image by using two independent sparse matrices. In the training section, the neural network is trained to find the mapping relationship between the speckle images and their corresponding original objects. Besides the model parameters, the decryption requires the use of the matrix keys, which avoids a situation where the DL-based cryptosystem is designed such that security relies entirely on the trained network itself. To the best of our knowledge, this is the first report using a single-wavelength, dual-channel system for diffuse optical encryption. Experimental results demonstrate the feasibility and good performance of the proposed scheme.

Two-Channel Detecting Sensor with Signal Cross-Correlation for FTIR Instruments.

Compared to the standard FTIR detection setup, our sensor ensures a higher signal-to-noise ratio (SNR) and lower signal standard deviation by reducing the uncorrelated noise components (e.g., thermal and 1/f noises of the detection module). Its construction is based on two-channel detection modules and a processing unit with implemented cross-correlation signal analyses. Each module was built of LWIR HgCdTe photodiodes and low-noise transimpedance amplifiers. the experiments demonstrated a decrease in a signal standard deviation of about 1.7 times with a 10 dB-improvement in the SNR. this result indicates our sensor's main benefit, especially in registered "weak" and noisy interferograms.

Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections

BackgroundEnterovirus (EV), parechovirus (HPeV), herpes simplex virus 1 and 2 (HSV1/2) are common viruses leading to viral central nervous system (CNS) infections which are increasingly predominant but exhibit deficiency in definite pathogen diagnosis with gold-standard quantitative PCR method. Previous studies have shown that droplet digital PCR (ddPCR) has great potential in pathogen detection and quantification, especially in low concentration samples.MethodsTargeting four common viruses of EV, HPeV, HSV1, and HSV2 in cerebrospinal fluid (CSF), we developed a multiplex ddPCR assay using probe ratio-based multiplexing strategy, analyzed the performance, and evaluated it in 97 CSF samples collected from patients with suspected viral CNS infections on a two-channel ddPCR detection system.ResultsThe four viruses were clearly distinguished by their corresponding fluorescence amplitude. The limits of detection for EV, HPeV, HSV1, and HSV2 were 5, 10, 5, and 10 copies per reaction, respectively. The dynamic range was at least four orders of magnitude spanning from 2000 to 2 copies per reaction. The results of 97 tested clinical CSF specimens were identical to those deduced from qPCR/qRT-PCR assays using commercial kits.ConclusionThe multiplex ddPCR assay was demonstrated to be an accurate and robust method which could detect EV, HPeV, HSV1, and HSV2 simultaneously. It provides a useful tool for clinical diagnosis and disease monitoring of viral CNS infections.

Bifunctional fluorescent probe for detecting and imaging hydrazine hydrate both in vitro and in vivo

Hydrazine (N2H4), an important alkaline agent with reducing activity, is widely used in agriculture and chemical industry. Although N2H4 is not produced in the human body, inhalation of trace N2H4 from the environment can cause serious harm to organisms. Therefore, it is important to accurately detect trace N2H4 for ensuring safety. Compared with the traditional N2H4 probe, dual-channel detection can provide better imaging resolution. The design of conventional dual-channel probes is based on the excited-state intramolecular proton transfer (ESIPT), which is limited by the choice of matrix. Developing novel probe that does not depend on the ESIPT mechanism and can provide two-channel detection is needed. Herein, a novel bifunctional fluorescent probe PTFD was designed and synthesized. Compared with the monofunctional fluorescent probe PTD, PTFD can achieve dual-channel detected N2H4 with a rapid response, excellent selectivity, and high sensitivity (detection limit of 0.08 µM). Importantly, PTD and PTFD can be applied for imaging of N2H4 in HeLa cells and zebrafish because of their good optical properties and low toxicity.

Quantum-enhanced super-sensitivity of a Mach–Zehnder interferometer with superposition of Schrödinger’s cat-like state and Fock state as inputs using a two-channel detection

We study the improvement in the phase sensitivity of a Mach–Zehnder interferometer using the superposition of Schrödinger’s cat-like state with the Fock state (SCFS) and the coherent state as inputs. With this setup, we examine the effect on phase sensitivity of the interferometer using a two-channel detection (TCD) scheme [Opt. Express 29, 95 (2021)OPEXFF1094-408710.1364/OE.413391] in which we take the optimal combination of the intensities of both output ports. We find better phase sensitivity under some conditions for this setup as compared to other known combinations of inputs such as squeezed vacuum and coherent states, vacuum and coherent states, etc. Therefore, we expect that the SCFS may be an alternative nonclassical resource for improvement in the phase sensitivity of a Mach–Zehnder interferometer, having potential application in quantum sensing.

A Versatile Multiple-Pass Raman System for Industrial Trace Gas Detection.

The fast and in-line multigas detection is critical for a variety of industrial applications. In the present work, we demonstrate the utility of multiple-pass-enhanced Raman spectroscopy as a unique tool for sensitive industrial multigas detection. Instead of using spherical mirrors, D-shaped mirrors are chosen as cavity mirrors in our design, and 26 total passes are achieved in a simple and compact multiple-pass optical system. Due to the large number of passes achieved inside the multiple-pass cavity, experiments with ambient air show that the noise equivalent detection limit (3σ) of 7.6 Pa (N2), 8.4 Pa (O2) and 2.8 Pa (H2O), which correspond to relative abundance by volume at 1 bar total pressure of 76 ppm, 84 ppm and 28 ppm, can be achieved in one second with a 1.5 W red laser. Moreover, this multiple-pass Raman system can be easily upgraded to a multiple-channel detection system, and a two-channel detection system is demonstrated and characterized. High utilization ratio of laser energy (defined as the ratio of laser energy at sampling point to the laser output energy) is realized in this design, and high sensitivity is achieved in every sampling position. Compared with single-point sampling system, the back-to-back experiments show that LODs of 8.0 Pa, 8.9 Pa and 3.0 Pa can be achieved for N2, O2 and H2O in one second. Methods to further improve the system performance are also briefly discussed, and the analysis shows that similar or even better sensitivity can be achieved in both sampling positions for practical industrial applications.

Broadening the high sensitivity range of squeezing-assisted interferometers by means of two-channel detection.

For a squeezing-enhanced linear (so-called SU(2)) interferometer, we theoretically investigate the possibility to broaden the phase range of sub-shot-noise sensitivity. We show that this goal can be achieved by implementing detection in both output ports, with the optimal combination of the detectors outputs. With this modification, the interferometer has the phase sensitivity independent of the interferometer operation point and, similar to the standard dark port regime, is not affected by the laser technical (excess) noise. Provided that each detector is preceded by a phase-sensitive amplifier, this sensitivity could be also tolerant to the detection loss.

Nonlinear absorption and scattering of a single plasmonic nanostructure characterized by x-scan technique

Nonlinear nanoplasmonics is a largely unexplored research area that paves the way for many exciting applications, such as nanolasers, nanoantennas, and nanomodulators. In the field of nonlinear nanoplasmonics, it is highly desirable to characterize the nonlinearity of the optical absorption and scattering of single nanostructures. Currently, the common method to quantify optical nonlinearity is the z-scan technique, which yields real and imaginary parts of the permittivity by moving a thin sample with a laser beam. However, z-scan typically works with thin films, and thus acquires nonlinear responses from ensembles of nanostructures, not from single ones. In this work, we present an x-scan technique that is based on a confocal laser scanning microscope equipped with forward and backward detectors. The two-channel detection offers the simultaneous quantification for the nonlinear behavior of scattering, absorption and total attenuation by a single nanostructure. At low excitation intensities, both scattering and absorption responses are linear, thus confirming the linearity of the detection system. At high excitation intensities, we found that the nonlinear response can be derived directly from the point spread function of the x-scan images. Exceptionally large nonlinearities of both scattering and absorption are unraveled simultaneously for the first time. The present study not only provides a novel method for characterizing nonlinearity of a single nanostructure, but also reports surprisingly large plasmonic nonlinearities.

Europium metal-organic framework for selective and sensitive detection of doxycycline based on fluorescence enhancement

Metal-organic framework is an extended coordination compound with repeating coordination entities and voids, which shows potential applications in gas storage, separation, catalysis, and etc. This work reports the synthesis of a new functional metal-organic framework of pyromellitic acid and europium, and its application in fluorescence sensing of doxycycline, a broad-spectrum polyketide antibiotic on the basis of fluorescence turn-on method. The metal-organic framework was initially non-fluorescent, however, with the addition of doxycycline, the system exhibited significant fluorescence enhancement at 526 nm and 617 nm, where both fluorescence intensities showed good linear correlations with the doxycycline concentration, suggesting the possibility for direct two-channel detection without any sensitization materials such as metal particles or surface-modified quantum dots. More interestingly, the unique fluorescence response of the system could discriminate doxycycline from other tetracycline antibiotics with high selectivity, and a limit of detection (LOD) was estimated to be 47 nM. It has been further demonstrated that the material has been successfully applied as sensing materials for selective determination of doxycycline in real samples of fish and urine.

Detector for rapid detection of trace Cu2+ in paddy water based on dual-wavelength quantum dot fluorescent probe

A novel device is designed for rapid detection of trace Cu2+ residues in paddy water using dual-wavelength quantum dot fluorescence probe. It includes three operation procedures: synthesis of dual-wavelength quantum dot, fluorescence excitation and acquisition of weak signal and signal conditioning. Based on the quenching principle of Cu2+ on quantum dot fluorescence, dual-wavelength quantum dot fluorescent probe can get lower limitation of detection. In addition, to improve the accuracy of testing results, two-channel detection was developed for avoiding interference. Experiment results demonstrate that the limitation for detection of trace Cu2+ in paddy water is 1.4 nM. The relationship between the Cu2+ concentration (x) and the ratio variation of dual wavelength (y) is y = 2.15 × exp(x/5.862) − 1.154 with the coefficient of determination (R2) of 0.997 and the detection time of 4 min. Meanwhile, compared to other heavy metal ions, nutrient and organic compounds, the presented detector is of good selectivity for Cu2+ in paddy water. The repetitive detection experiments show that the developed device has high repeatability with relative standard deviation of 0.14%. The device can be used for quick and accurate detection of trace Cu2+ residues in paddy water with some specific advantages of high sensitivity, excellent selectivity and high repeatability.

Polarized object detection in crabs: a two-channel system.

Many animal species take advantage of polarization vision for vital tasks such as orientation, communication and contrast enhancement. Previous studies have suggested that decapod crustaceans use a two-channel polarization system for contrast enhancement. Here, we characterize the polarization contrast sensitivity in a grapsid crab. We estimated the polarization contrast sensitivity of the animals by quantifying both their escape response and changes in heart rate when presented with polarized motion stimuli. The motion stimulus consisted of an expanding disk with an 82deg polarization difference between the object and the background. More than 90% of animals responded by freezing or trying to avoid the polarized stimulus. In addition, we co-rotated the electric vector (e-vector) orientation of the light from the object and background by increments of 30deg and found that the animals' escape response varied periodically with a 90deg period. Maximum escape responses were obtained for object and background e-vectors near the vertical and horizontal orientations. Changes in cardiac response showed parallel results but also a minimum response when e-vectors of object and background were shifted by 45deg with respect to the maxima. These results are consistent with an orthogonal receptor arrangement for the detection of polarized light, in which two channels are aligned with the vertical and horizontal orientations. It has been hypothesized that animals with object-based polarization vision rely on a two-channel detection system analogous to that of color processing in dichromats. Our results, obtained by systematically varying the e-vectors of object and background, provide strong empirical support for this theoretical model of polarized object detection.

A CsI low-temperature detector for dark matter search

Cryogenic detectors have a long history of success in the field of rare event searches. In particular scintillating calorimeters are very suitable detectors for this task since two signals are induced by a particle interaction in a scintillating crystal. The thermal signal provides a precise measurement of the deposited energy while the simultaneously measured scintillation light signal yields particle discrimination as the amount of produced scintillation light depends on the nature of the interacting particle. We investigate the calorimetric properties and background rejection capabilities of two large CsI (undoped) crystals (∼122 g each) operated as scintillating calorimeters at milli-Kelvin temperatures. Furthermore, we discuss the feasibility of this detection approach towards a future background-free dark matter experiment based on alkali halide crystals, with active particle discrimination via the two-channel detection.

The Designation and Simulation of Amplifying and Shaping Circuit of SAW Gas Sensor Signal

In this paper, a kind of amplifying and shaping circuit was designed according to the characteristics of high frequency and small amplitude of surface acoustic wave (SAW) gas sensor signal. The circuit is applied to two-channel mixing detection method to solve the problem that backend devices cannot identify sensor signal. The whole circuit is simple in structure and has very good shaping effect on voltages signal of millivolt level, meeting the need of the sensor of gas detection.

Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.

Airy beams maintain their intensity profiles over a large propagation distance without substantial diffraction and exhibit lateral bending during propagation1-5. This unique property has been exploited for micromanipulation of particles6, generation of plasma channels7 and guidance of plasmonic waves8, but has not been explored for high-resolution optical microscopy. Here, we introduce a self-bending point spread function (SB-PSF) based on Airy beams for three-dimensional (3D) super-resolution fluorescence imaging. We designed a side-lobe-free SB-PSF and implemented a two-channel detection scheme to enable unambiguous 3D localization of fluorescent molecules. The lack of diffraction and the propagation-dependent lateral bending make the SB-PSF well suited for precise 3D localization of molecules over a large imaging depth. Using this method, we obtained super-resolution imaging with isotropic 3D localization precision of 10-15 nm over a 3 μm imaging depth from ∼2000 photons per localization.

A two-channel detection method for autofluorescence correction and efficient on-bead screening of one-bead one-compound combinatorial libraries using the COPAS fluorescence activated bead sorting system

One-bead one-compound combinatorial library beads exhibit varying levels of autofluorescence after solid phase combinatorial synthesis. Very often this causes significant problems for automated on-bead screening using TentaGel beads and fluorescently labeled target proteins. Herein, we present a method to overcome this limitation when fluorescence activated bead sorting is used as the screening method. We have equipped the COPAS bead sorting instrument with a high-speed profiling unit and developed a spectral autofluorescence correction method. The correction method is based on a simple algebraic operation using the fluorescence data from two detection channels and is applied on-the-fly in order to reliably identify hit beads by COPAS bead sorting. Our method provides a practical tool for the fast and efficient isolation of hit beads from one-bead one-compound library screens using either fluorescently labeled target proteins or biotinylated target proteins. This method makes hit bead identification easier and more reliable. It reduces false positives and eliminates the need for time-consuming pre-sorting of library beads in order to remove autofluorescent beads.

Isotropic 3D Super Resolution Imaging with Self-Bending Point Spread Function

We introduce a self-bending point spread function (SB-PSF) based on Airy beams for three-dimensional (3D) super-resolution fluorescence imaging. We designed a side-lobe-free SB-PSF for the incoherent fluorescence emission and implemented a two-channel detection scheme for the SB-PSF to enable unambiguous 3D localization of fluorescent molecules. The lack of diffraction and the propagation-dependent lateral bending make the SB-PSF ideal for precise 3D localization of molecules over a large imaging depth. Using this SB-PSF, we demonstrated super-resolution imaging with isotropic localization precisions of 10-15 nm in all three dimensions over a 3 μm imaging depth without sample scanning.

Characterizing quantum microwave radiation and its entanglement with superconducting qubits using linear detectors

Recent progress in the development of superconducting circuits has enabled the realization of interesting sources of nonclassical radiation at microwave frequencies. Here, we discuss field quadrature detection schemes for the experimental characterization of itinerant microwave photon fields and their entanglement correlations with stationary qubits. In particular, we present joint state tomography methods of a radiation field mode and a two-level system. Including the case of finite quantum detection efficiency, we relate measured photon field statistics to generalized quasiprobability distributions and statistical moments for one-channel and two-channel detection. We also present maximum-likelihood methods to reconstruct density matrices from measured field quadrature histograms. Our theoretical investigations are supported by the presentation of experimental data, for which microwave quantum fields beyond the single-photon and Gaussian level have been prepared and reconstructed.

Fiber-optic multiphoton flow cytometry in whole blood and in vivo

Circulating tumor cells in the bloodstream are sensitive indicators for metastasis and disease prognosis. Circulating cells have usually been monitored via extraction from blood, and more recently in vivo using free-space optics; however, long-term intravital monitoring of rare circulating cells remains a major challenge. We demonstrate the application of a two-photon-fluorescence optical fiber probe for the detection of cells in whole blood and in vivo. A double-clad fiber was used to enhance the detection sensitivity. Two-channel detection was employed to enable simultaneous measurement of multiple fluorescent markers. Because the fiber probe circumvents scattering and absorption from whole blood, the detected signal strength from fluorescent cells was found to be similar in phosphate-buffered saline (PBS) and in whole blood. The detection efficiency of cells labeled with the membrane-binding dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindoldicarbocyanine, 4-chlorobenzenesulfonate (DiD) was demonstrated to be the same in PBS and in whole blood. A high detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was also demonstrated. To characterize in vivo detection, DiD-labeled untransfected and GFP-transfected cells were injected into live mice, and the cell circulation dynamics was monitored in real time. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed ex vivo in whole blood.

Two-photon in vivo flow cytometry using a fiber probe.

We have demonstrated the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in vitro and in vivo. We conducted two-channel detection to measure fluorescence at two distinct wavelengths simultaneously. Because the scattering and absorption problems from whole blood were circumvented by the fiber probe, the detected signal strength from the cells were found to be similar in PBS and in whole blood. We achieved the same detection efficiency of the membrane-binding lipophilic dye DiD labeled cells in PBS and in whole blood. High detection efficiency of green fluorescent protein (GFP)-expressing cells in whole blood was demonstrated. DiD-labeled untransfected and GFP-transfected cells were injected into live mice and the circulation dynamics of the externally injected cells were monitored. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed in whole blood.