Tunica Adventitia Research Articles

Encapsulation of Plant Biocontrol Bacteria with Alginate as a Main Polymer Material.

One of the most favored trends in modern agriculture is biological control. However, many reports show that survival of biocontrol bacteria is poor in host plants. Providing biocontrol agents with protection by encapsulation within external coatings has therefore become a popular idea. Various techniques, including extrusion, spray drying, and emulsion, have been introduced for encapsulation of biocontrol bacteria. One commonly used biopolymer for this type of microencapsulation is alginate, a biopolymer extracted from seaweed. Recent progress has resulted in the production of alginate-based microcapsules that meet key bacterial encapsulation requirements, including biocompatibility, biodegradability, and support of long-term survival and function. However, more studies are needed regarding the effect of encapsulation on protective bacteria and their targeted release in organic crop production systems. Most importantly, the efficacy of alginate use for the encapsulation of biocontrol bacteria in pest and disease management requires further verification. Achieving a new formulation based on biodegradable polymers can have significant effects on increasing the quantity and quality of agricultural products.

Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension

Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes (DM2). Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, n = 6) versus hypertensive non-diabetic patients (AH group, n = 34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test. In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (p = 0.004) and B cells (p = 0.025) in the tunica intima and tunica media (p = 0.049 and p = 0.007, respectively), and fewer plasma cells in the tunica media (p = 0.024) and tunica adventitia (p = 0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (p = 0.025). Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall and more collagen fibers in tunica adventitia.

Investigation of Hemodynamic Receptors of the Internal Carotid Artery Segments

ObjectivesInternal carotid artery (ICA), the main artery of the brain, passes through the cavernous sinus (CS) which forms one of these venous pools. During this transition, while there is arterial blood in the lumen of ICA, its outer surface is in contact with venous blood from the brain. Herein, we aimed to detect the receptor differences of ICA in this highly specialized anatomical region of the skull base.MethodsWe performed the study on 10 human cadavers and searched CGRPR, TRP12, ASIC3 and ACTHR receptors via immunostaining using laser scanning confocal microscopy.ResultsWe determined TRP12 receptor positive in the tunica media and tunica adventitia layers of the cavernous segment of ICA. We did not detect similar positivity in the cervical part of the ICA. In the receptor scan we made in terms of CGRPR, while we detected positivity in the tunica media layer of the cavernous segment, we found positivity in the tunica intima layer of the cervicalis segment of the ICA. We did not detect any positivity for ASIC3 and ACTHR receptors in both parts of the ICA.ConclusionsAs a result, we observed various differences in receptors between ICA segments. While the outer surface of the ICA in the cervical region did not show any receptor positivity, we detected TRP12 receptor positivity along the tissue contour of vessel in the CS. We assume that it may provide a new perspective on pathologies of the CS/ICA and preservation of brain hemodynamics for clinicians.

The Efficacy of Hydrodilatation for the Prevention of Vasospasm following Microsurgical Anastomosis.

Vasospasm is a major problem following microsurgical reconstruction which can result in the partial or complete loss of the flap tissue. The aim of this study was to investigate the efficiency of hydrodilatation for the prevention of vasospasm. Thirty male Wistar rats were used for this experimental study. Femoral arteries of were exposed, photographed, and transected. In group 1, group 2, and group 3 papaverine solution, hydrodilatation, and minimal mechanical dilatation (control group) was performed, respectively. The anastomosis was completed and the arteries were photographed again 10 minutes after completion of the anastomosis. Following 7-day period samples for transmission electron microscopy (TEM) and light microscopy were obtained. The mean vessel diameters prior to transection were 0.43, 0.45, and 0.52 mm in the papaverine, hydrodilatation, and control groups, respectively. The mean vessel diameter 10 minutes following the completion of anastomosis was 0.76, 0.75, and 0.51 mm in the papaverine, hydrodilatation, and control groups, respectively. Median score for papaverine group regarding histological parameters of regular endothelial lining and lumen, neutrophil infiltration, vascular congestion, and edema in tunica adventitia was 2, 3, 2, and 3 positive, respectively. Median score for the papaverine group regarding histological parameters of regular endothelial lining and lumen, neutrophil infiltration, vascular congestion, and edema in tunica adventitia was 3, 3, 3, and 3 positive, respectively. All the histological scores were negative in the control group. The difference between the control group and the experiment groups 1 and 2 was significant regarding all four histological parameters (p < 0.05). Hydrodilatation and papaverine application were both effective in preventing vasospasm following microsurgical intervention but papaverine caused slightly less damage to the endothelial lining and less edema in the tunica adventitia when compared with the hydrodilatation. Hydrodilatation group showed a vasodilatory effect that was statistically similar to that of papaverine, which has a proven efficacy.

Traumatic Pseudoaneurysm of the Superficial Temporal Artery.

Traumatic pseudoaneurysm (PA) of the superficial temporal artery is a rare but potentially dangerous condition that can occur after blunt facial trauma. Although true aneurysms are characterized by dilation or bulging of all three intact layers of the vessel wall, PAs are a disruption of the vessel in which the blood is only bound either by the outermost tunica adventitia or by adjacent soft tissues. If the wall subsequently ruptures, patients are at risk of hemorrhaging. The superficial temporal artery is especially susceptible to PA formation among facial vasculature (1).

Polyethylene Films Coated with Antibacterial and Antiviral Layers Based on CO2 Extracts of Raspberry Seeds, of Pomegranate Seeds and of Rosemary

The main goal of the work was to create an internal coating based on super critical CO2 extracts of raspberry seeds, pomegranate seeds and rosemary that could be active against chosen bacterial strains. Additionally, the synergistic effect of these substances in the coating were then analysed. The next goal of the work was to demonstrate the antiviral activity of the coatings against phi6 bacteriophage particles (airborne viruses surrogate). The results of the study indicated that three coatings containing a mixture of extracts showed bacteriolytic activity against S. aureus cells and bacteriostatic activity against E. coli and B. subtilis strains. Two coatings showed bacteriolytic activity against a P. syringae strain. As a result of the experiments, a synergistic effect was noted in the active additives/compounds in the coatings. These coatings may be used as internal coatings for packaging films to extend the shelf life of selected food products. All seven coatings may also be used as external coatings with antiviral activity, as these coatings demonstrated significant effects on the phi6 phage, selected as a surrogate for airborne viruses, e.g., coronaviruses. It could be concluded that coatings I–VII will also show antiviral effects on SARS-CoV-2 particles.

English

BACKGROUND Human umbilical cord contains two arteries and one vein with their tunica intima and tunica media layers. The role of tunica adventitia is fulfilled by Wharton’s jelly, a mucoid connective tissue. The function of Wharton’s jelly is to prevent the vessels from compression and torsion which is essential for foetal development. The purpose of the study was to estimate the importance of Wharton’s jelly in the growth of the foetus. METHODS Umbilical cord tissue collected from each case was immediately put in 10 % formalin for fixation. Slides were then stained with Haematoxylin and Eosin. These slides were then read under light microscopy and measurements were taken using a photomicrograph. Wharton’s jelly area was calculated by subtracting the total vessel area from the umbilical cord area. RESULTS The histological measurements of umbilical vessels include the external diameter, lumen diameter, wall thickness, thickness of tunica intima and tunica media, and the area. The mean area of the umbilical cord was 35.73 ±23.04 mm2 (Mean ± SD) and the mean area of the Wharton’s jelly was 29.74 ± 19.26 mm2. There was a significant difference in the external diameter and wall thickness of the umbilical artery. Analyses showed that there was a significantly (P &lt; 0.01) increased external diameter and wall thickness of umbilical artery in normal cases, compared to single umbilical artery cases. CONCLUSIONS There was a significant positive correlation between the gestational age and the external diameter of the umbilical cord. There was a significant difference in the external diameter of the umbilical cord between SUA cases (4.45 mm) and the other foetuses with normal umbilical cord (6.53 mm). There was a significantly increased external diameter, lumen diameter, wall thickness and area of umbilical vein in normal cases, compared to single umbilical artery cases. There was a significantly increased area of umbilical cord and area of Wharton’s jelly in normal umbilical cord foetuses than foetuses with a single umbilical artery. KEY WORDS Foetus, Umbilical Cord, Wharton’s Jelly, Umbilical Artery, Umbilical Vein, Light Microscopy

Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair.

Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aortic wall responds to the implantation of a stentgraft and EL is mostly uncertain. We present a pilot study to identify peptide signatures and gain new insights in pathophysiological alterations of the aortic wall after EVAR using matrix-assisted laser desorption or ionization mass spectrometry imaging (MALDI-MSI). In course of or accompanying an open aortic repair, tissue sections from 15 patients (TAA = 5, AAA = 5, EVAR = 5) were collected. Regions of interest (tunica media and tunica adventitia) were defined and univariate (receiver operating characteristic analysis) statistical analysis for subgroup comparison was used. This proof-of-concept study demonstrates that MALDI-MSI is feasible to identify discriminatory peptide signatures separating TAA, AAA and EVAR. Decreased intensity distributions for actin, tropomyosin, and troponin after EVAR suggest impaired contractility in vascular smooth muscle cells. Furthermore, inability to provide energy caused by impaired respiratory chain function and continuous degradation of extracellular matrix components (collagen) might support aortic wall destabilization. In case of EL after EVAR, this mechanism may result in a weakened aortic wall with lacking ability to react on reinstating pulsatile blood flow.

Perineural Invasion Should Be Regarded as an Intermediate-Risk Factor for Recurrence in Surgically Treated Cervical Cancer: A Propensity Score Matching Study.

Background Perineural invasion (PNI) is considered as a poor prognostic factor in cervical cancer, but there has been no postoperative adjuvant therapy for it, because whether it belongs to high- or intermediate-risk factors has not been determined, this study intends to provide evidences to solve this problem. Methods We conducted a retrospective analysis of cervical cancer patients who underwent radical surgery and be reported PNI from January 2012 to June 2017 at the Sun Yat-sen University Cancer Center. After 1 : 1 propensity score matching (PSM), a group of patients without PNI was matched according to the clinical pathological features. Postoperative pathological parameters and prognosis were evaluated between the PNI and the matched groups. Results 1836 patients were screened, of which 162 (8.8%) diagnosed as stages IB1 to IIB reported PNI. Comparing to the matched group, more PNI (+) patients had deep outer cervix stromal invasion, cervical tunica adventitia invasion, positive lymph nodes, and positive margins. Among patients without high-risk factors, PNI (+) patients had worse 3-year overall survival (90.8% vs. 98.1%, P = 0.02), PNI (+) patients with single intermediate-risk factor and PNI (-) patients who meet with SEDLIS criteria had similar progress free survival (P = 0.63) and overall survival (P = 0.63), even similar survival curves. Conclusion PNI is related to a worse overall survival among cervical cancer patients without high-risk factors and play the role as an intermediate-risk factor.

Effect of Telmisartan on Arsenic-Induced (Sub-chronic) Perturbations in Redox Homeostasis, Pro-inflammatory Cascade and Aortic Dysfunction in Wistar Rats.

An experimental study was conducted in male Wistar rats to explore the antioxidant potential of telmisartan (an AT1 receptor blocker) to overcome arsenic ('As')-induced perturbations in redox homeostasis pro-inflammatory cytokines, prostaglandin-E2 levels and aortic dysfunction in Wistar rats. Wistar rats were randomly divided into four groups of six each. Group-I served as untreated control, while group-II received sodium (meta) arsenite (NaAsO2) (10mg/kg b.wt. p.o) for a period of 60days. Experimental rats in group-III received treatment similar to group-II, but in addition received telmisartan (with 1% aqueous solution of Tween 80) @ 10mg/kg b.wt. (p.o) for a similar duration, while rats in group-IV received telmisartan alone. Arsenic exposure resulted in significant (p < 0.05) elevation in the levels of superoxide anion ([Formula: see text]) radicals (control: 768.20 ± 126.77 vs group-II: 1232.75 ± 97.85pmol of NBT reduced/min/mg protein). Telmisartan administration showed significant (p < 0.05) reduction in [Formula: see text] generation (815.34 ± 43.41pmol of NBTreduced/min/mg protein). Sub-chronic exposure to 'As' significantly (p < 0.05) decreased the activities of SOD, CAT, GPx and GR activity and GSH levels in the aorta, thus induced lipid peroxidation (LPO) measured as measured in terms of thiobarbituric acid reactive substances (TBARS) called as malondialdehyde (MDA). However, the administration of telmisartan effectively countered the LPO (24.03 ± 1.18nmol of MDA/g) on account of restoring the levels of aforesaid antioxidant defense system. Telmisartan administration effectively attenuated the 'As'-induced surge in pro-inflammatory cytokines (viz., IL-1β, IL-6 and TNF-α) levels, as well as countered the activity of cyclooxygenase (COX2) as indicated by a significant (p < 0.05) decrease in PGE2 level in the aorta. In addition to it, there was a significant (p < 0.05) decrease in plasma angiotensin II (Ang-II) levels in experimental rats receiving telmisartan. Quantitative RT-PCR studies revealed that sub-chronic exposure to 'As' upregulated the Nox2 mRNA expression, but there was a 1.2-fold reduction in expression level upon co-administration of telmisartan. Histopathological examination revealed marked recovery from 'As'-induced disruption of tunica adventitia and loss of connective tissue in experimental rats receiving telmisartan. The study concludes that telmisartan can overcome aortic dysfunction induced by sub-chronic exposure to arsenic through drinking water in experimental rats through restoration of redox balance, attenuation of pro-inflammatory cytokines and mediators and downregulation of Nox2 mRNA expression.

Bone-forming perivascular cells: Cellular heterogeneity and use for tissue repair.

Mesenchymal progenitor cells are broadly distributed across perivascular niches-an observation conserved between species. One common histologic zone with a high frequency of mesenchymal progenitor cells within mammalian tissues is the tunica adventitia, the outer layer of blood vessel walls populated by cells with a fibroblastic morphology. The diversity and functions of (re)generative cells present in this outermost perivascular niche are under intense investigation; we have reviewed herein our current knowledge of adventitial cell potential with a somewhat narrow focus on bone formation. Antigens of interest to functionally segregate adventicytes are discussed, including CD10, CD107a, aldehyde dehydrogenase isoforms, and CD140a, among others. Purified adventicytes (such as CD10+ , CD107alow , and CD140a+ cells) have stronger osteogenic potential and promote bone formation in vivo. Recent bone tissue engineering applications of adventitial cells are also presented. A better understanding of perivascular progenitor cell subsets may represent a beneficial advance for future efforts in tissue repair and bioengineering.

Technological Advances in Penile Implant Surgery

BackgroundDuring the last century, surgical management of erectile dysfunction has evolved from an experimental concept to a core treatment modality with widespread use among the men's health community. Over time, innovations in materials, mechanical design elements, device coatings, and surgical technique have provided patients with low-risk, reliable, and reproducible erectile function with high satisfaction rates. AimTo provide a foundation for future innovation by improving understanding of historical penile prosthetics and the rationale behind incremental technological improvements for the contemporary Men's Health physician. MethodsLiterature review was conducted to generate a comprehensive review of historical technological innovations in penile implant surgery. Companies with FDA approved penile prosthetics in use in the United States were contacted for information regarding technological innovations in the past and future devices in development. A separate literature review was performed to identify any significant future device design elements being tested, even in the ex vivo setting, which may have future clinical applications. OutcomesTechnological innovations in penile implant surgery were described. ResultsCurrent options for the prosthetic surgeon include malleable penile prostheses (MPP), self-contained (2-piece) inflatable penile prostheses, and multicomponent (3-piece) inflatable penile prostheses. Current MPPs consist of a synthetic coated solid core which allow for manipulation of the penis for concealability while maintaining sufficient axial rigidity to achieve penetration when desired. Multi-component (3-Piece) IPPs currently include the Coloplast Titan and Boston Scientific/AMS 700 which consist of a fluid reservoir, intrascrotal pump, and intracavernosal cylinders. The devices have undergone numerous design updates to the cylinders, pump, reservoir, tubing, and external coatings to increase reliability and decrease short- and long-term complications. Clinical ImplicationsFuture innovations in penile prosthetic surgery seek to broaden the indications and applicability to the transgender community and improve both safety and functionality for patient and partner. Strengths & LimitationsThe review is limited primarily to penile prosthetics approved for current or historical clinical use in the United States and may not be representative of the global prosthetic environment. Additionally, the research and development of future innovations, particularly those provided by device manufacturers, is likely limited by non-disclosure to maintain a competitive advantage. ConclusionsPenile prosthetic surgery will undoubtedly remain integral to the treatment of erectile dysfunction, and education regarding the current state of technological innovation will empower the prosthetic surgeon and biomedical engineering community to improve contemporary patient care and drive the development of the next generation of implantable penile prosthetics.Barnard JT, Cakir OO, Ralph D, et al. Technological Advances in Penile Implant Surgery. J Sex Med 2021;18:1158–1166.

Ischemia-reperfusion injury to coronary arteries: Comprehensive microscopic study after reperfused myocardial infarction

BackgroundCoronary arteries supply oxygen and nutrients to the heart. We evaluated the dynamics of microscopic damage throughout the ischemia-reperfusion process in the wall of coronary arteries following myocardial infarction (MI). MethodsIn a swine model of reperfused MI, animals were divided into one control and four MI groups: 90-min ischemia without reperfusion, or followed by one minute, one week or one month reperfusion. Left anterior descending (LAD; infarct-related artery) and control right coronary arteries (RCA) were isolated. Taking the balloon inflation region as a reference, we isolated the proximal and distal LAD areas, performing histological staining and immunohistochemistry. ResultsAlthough mild changes in tunica intima were observed during ischemia, an almost complete absence of endothelium, and abnormal breaks in the internal elastic layer were found post-revascularization. In tunica media, increased thickness was observed soon after reperfusion, whereas larger thickness, disorganized muscle cell distribution and edema were found one week after reperfusion. This damage was more pronounced in distal rather than proximal LAD, whereas no changes were detected in RCA. In the tunica adventitia, vasa vasorum density decayed during ischemia in both LAD regions, but was restored after one month. Leukocyte adhesion to the artery was observed post-revascularization, developing into a massive presence in the three layers one week post-reperfusion. ConclusionsIschemia-reperfusion can itself induce damage in the wall of the epicardial coronary artery, becoming more pronounced in the region distal to balloon inflation. Exploring these abnormalities will provide insight into the pathophysiology of coronary circulation and MI.

P0137 - Internal and external metal-based coatings to prevent uropathogen adhesion on stents

P0137 - Internal and external metal-based coatings to prevent uropathogen adhesion on stents

Histomorphometric and histochemical characteristics of the oesophagus of the greater mouse-eared Bat, Myotis myotis (Borkhausen, 1797).

The aim of this study was to examine the histomorphometric and histochemical structure of oesophagus of the wild-caught greater mouse-eared bat M.myotis. For this purpose, 10 specimens of oesophagus were collected and processed. The oesophagus wall of M.myotis is composed of four layers: tunica mucosa, tunica submucosa, tunica muscularis and tunica adventitia. The mucosa is covered by a non-keratinized stratified squamous epithelium. There were no glands in the wall of the oesophagus. Tunica muscularis is composed of two muscle layers: internal circular muscular layer and an external longitudinal muscular layer. Histochemical studies revealed that the oesophagus was AB/PAS and PAS positive indicating the presence of acidic and neutral mucosubstances. AB-PAS staining shows that glycoproteins were predominant in the all examined layers of the oesophagus. Because of the absence of oesophageal glands in M.myotis, positive reaction with AB-PAS staining may be due to secretion of salivary glands. Absence of oesophageal glands reflects the functional adaptation as high food passage rate for the insectivorous feeding habits of animals.

A High‐Sensitivity Refractive Index Sensor Based on an External Gold Coating of a Square‐Lattice Photonic Crystal Fiber

A high‐sensitivity square‐lattice photonic crystal fiber (PCF) refractive index (RI) sensor based on an external gold coating is proposed. This design eliminates the limitations of selective coating and liquid penetration in the micrometer holes, and avoids the side polishing of D‐type fibers. The effects of air hole diameter and gold film thickness on the sensing performance are studied in detail using the full vector finite element method (FV‐FEM). In addition, the sensing performance of the gold‐coated PCF under the optimized parameters is compared with that of PCF with the same air hole diameter and a gold grating. The results show that the proposed PCF has a higher structural tolerance to the air hole diameter, which improves the manufacturing feasibility. Moreover, the gold‐coated PCF has a maximum sensitivity of 31 000 nm RIU−1 and a resolution of 3.23 × 10−6 RIU in the RI measurement range of 1.34–1.49. The maximum sensitivity of the gold‐grating PCF with lower loss is 30 000 nm RIU−1 in the RI detection range of 1.38–1.46, and the resolution is 3.33 × 10−6 RIU. Low loss is beneficial for extending the length of the fiber, which is convenient for practical implementation. Thus, it has potential applications in biomolecular and biochemical analysis.

Towards selective laser paint stripping using shock waves produced by laser-plasma interaction for aeronautical applications on AA 2024 based substrates

Laser stripping is a process which typically includes different forms of ablation phenomena. The presented work investigates a mechanical stripping process using high pressure laser-induced shock waves in a water confined regime. Power density is studied as a parameter for selective laser stripping on painted specimens and for adhesion relations with single layer epoxy targets. A flashlamp-pumped Nd:YAG laser with fixed spot size (4 mm) is shot on single layer epoxy and several layers of polymeric paint applied on a AA 2024-T3 (Aluminium) substrate. After laser treatment, samples are investigated with optical microscopy, profilometer and chemical analysis (FTIR & TGA). The results show that selective laser stripping is possible between different layers of external aircraft coatings and without any visual damage on the substrate material. In parallel to the experimental work, a numerical model has been developed to explain the background of the physical mechanisms and to qualitatively evaluate the detailed stress analysis and interfacial failure simulation for a single layer of epoxy on an aluminium substrate. The predicted failure patterns agree with the surfaces of the tested specimens observed by a microscope.

Shedding Damage Detection of Metal Underwater Pipeline External Anticorrosive Coating by Ultrasonic Imaging Based on HOG + SVM

Underwater pipelines are the channels for oil transportation in the sea. In the course of pipeline operation, leakage accidents occur from time to time for natural and man-made reasons which result in economic losses and environmental pollution. To avoid economic losses and environmental pollution, damage detection of underwater pipelines must be carried out. In this paper, based on the histogram of oriented gradient (HOG) and support vector machine (SVM), a non-contact ultrasonic imaging method is proposed to detect the shedding damage of the metal underwater pipeline external anti-corrosion layer. Firstly, the principle of acoustic scattering characteristics for detecting the metal underwater pipelines is introduced. Following this, a HOG+SVM image-extracting algorithm is used to extract the pipeline area from the underwater ultrasonic image. According to the difference of mean gray value in the horizontal direction of the pipeline project area, the shedding damage parts are identified. Subsequently, taking the metal underwater pipelines with three layers of polyethylene outer anti-corrosive coatings as the detection object, an Autonomous Surface Vehicle (ASV) for underwater pipelines defect detection is developed to verify the detection effect of the method. Finally, the underwater ultrasonic image which used to detect the metal underwater pipeline shedding damage is obtained by acoustic sensor. The results show that the shedding damage can be detected by the proposed method. With the increase of shedding damage width, the effect of pipeline defect location detection is better.

Microscopic and morphometric study in trachea and lungs of adult Iraqi pigeon (Columba livia)

The study applied light to investigate the microscopic and macroscopic results of the trachea and lungs in adult Iraqi pigeons (Columba livia). Age of the pigeons was (8-12) months. Our study was carried out 12 adult pigeons. These pigeons were supplement from local market of Baghdad city, Iraq. The morphological study of adult pigeon observed that the trachea included long tube of cartilaginous structure is extended from larynx until enters to left and right lungs with runs alongside the esophagus inside the thoracic cavity. This trachea was characterized by flexible bulk, non folded ring and numerous series of thin complete cartilage ring. The statistics analysis was used for grossly morphological organs, where was number of thin cartilage rings average range (67-70) complete rings. The study used the morphometric measurements for length of the trachea was (10±0.6) cm. and diameter of ring lumen about (0.6±0.12) cm.. Either the lungs results of adult pigeons were showed pink color, spongy mass and trapezium- like. The lungs were located inside thoracic cavity and connected by ribs with appearance costal impressions on the dorsal surface of the lungs. The morphometric study were included statistic analysis of lungs, weight of lungs were about (6±0.01) mg. and length was ( 3.32±0.2) cm. . Histological study showed tracheal ring of Columba livia was thin complete. The study observed histological layers included tunica mucosa, submucosa and adventitia, the first portion of mucosa is called epithelia, it’s characteristic by ciliated pseudostratified columnar epithelium with goblet cells, lamina properia has loose connective tissue which it is rich blood vessels. Submucosa is a beneath layer of mucosa, and contains tracheal cartilaginous tube, large blood vessels and numerous seromucous glands. Pigeon’s lungs were formed than parabronchi, it’s located in center of pulmonary lobule and separated by septa (connective tissue) named interlobular wall, which is originated from secondary bronchi and united with other. The epithelia lined the parabronchi by simple squeomous cells to help gaseous exchange inside the lungs .

Laparoscopic Ablation System for Complete Circumferential Renal Sympathetic Denervation.

The catheter-based renal denervation (RDN) showed promising results for patients in lowering BP, but there were also many non-responders. One of the possible reasons was the incomplete neural ablation due to the ablation of renal nerves at random sites resulting in asymmetric innervation patterns along the renal artery. We developed a laparoscopic ablation system that is optimized for complete RDN regardless of renal arterial innervation and size. To demonstrate its effectiveness, we evaluated the system using computational simulation and 28-day survival model using pigs. The ablations were focused around the tunica externa, and the ablation patterns could be predicted numerically during RDN treatment. In the animal study, the mean reduction of systolic BP and diastolic BP in the bilateral main renal arteries was 22.8 mmHg and 14.4 mmHg (P<0.001), respectively. The respond to immunostaining targeting tyrosine hydroxylase was significantly reduced at treatment site (108.2 ± 7.5 (control) vs. 63.4 ± 8.7 (treatment), P<0.001), and an increased degree of sympathetic signals interruption to kidneys was associated with the efficacy of RDN. The laparoscopic ablation system achieved complete circumferential RDN at the treatment site and could numerically predict the ablation patterns. These findings clearly suggest that the proposed system can significantly improve the RDN effectiveness by reducing the variation to the percentage of injured nerves and open up a new opportunity to treat uncontrolled hypertension.