A key component fumarine of Sijunzi decoction induces necroptosis in clear cell renal cell carcinoma potentially via targeting SLC6A3, OPRD1 and KDR.

CuET inhibits Ewing sarcoma of bone progression through modulation of the ESM1-MAPK/ERK signaling axis.

Umbilical cord mesenchymal stem cell exosomes in breast cancer. False hope or a real solution?

MMP10 is highly expressed in an osteosarcoma stem cell model and predicts poor prognosis.

Sishen Wan suppresses colon cancer through dual inhibition of PI3K/AKT/mTOR and STAT3-mitophagy pathways: Network pharmacology and experimental validation.

Pancreatic ductal adenocarcinoma (PDAC), the main pancreatic cancer type, was highly aggressive and lethal. Studies showed that the epidermal growth factor receptor pathway substrate 8-Like protein 3 (EPS8L3) was significantly upregulated in PDAC. This study aimed to explore how EPS8L3 promoted PDAC progression. First, bioinformatics analysis, qRT-PCR, and western blot (WB) techniques were utilized to ascertain the expression profile of EPS8L3 in clinical samples and cells of PDAC. Subsequently, EdU proliferation assays, cell apoptosis detection, glycolysis assay kits, in vivo xenograft tumor experiments, and immunohistochemical (IHC) staining were conducted to explore the impact of EPS8L3 silencing on PDAC cell proliferation, apoptosis, glycolytic pathway, and tumor growth in vivo. Meanwhile, flow cytometry was employed to analyze the expression of CD163, a marker of macrophage M2 polarization. Furthermore, with the aid of JASPAR and GEPIA websites, combined with chromatin immunoprecipitation (Ch-IP) experiments and dual luciferase reporter gene experiments, the interaction between transcription factor AP-2α (TFAP2A) and EPS8L3 was further confirmed. Finally, a rescue experiment was performed with EPS8L3 overexpression in TFAP2A-knockdown cells to validate the potential impact of EPS8L3 on TFAP2A function. EPS8L3 was highly expressed in PDAC tumors and PDAC cells, and its silencing effectively inhibited the proliferation of PDAC cells and promoted their apoptosis. Furthermore, the glycolytic pathway in PDAC cells, tumor growth in vivo, and M2 polarization of macrophages were also blocked by EPS8L3 knockdown. TFAP2A interacted with EPS8L3 and positively regulated its expression. Overexpression of EPS8L3 restored the effects of TFAP2A knockdown on PDAC cell progression. TFAP2A positively regulated EPS8L3 to facilitate M2 polarization of macrophages and the malignant progression of PDAC cells.

Targeting HSPB1 inhibits tumor growth and abrogates Treg-mediated tumor immunosuppression.

The Role of Interferon-γ-Induced Granzyme B in Enhancing Antitumour Immune Responses in Oral Squamous Cell Carcinoma.

Multifunctional exosome-driven tumor immunotherapy sensitization: converting intratumoral bacteria into antitumor fighters.

Modeling tumor progression in heterogeneous microenvironments: A cellular automata approach.

The MCM/Lys-Cys nanodevices for the efficient gene delivery: An approach towardsMCP1gene manipulation using CRISPR technology.

Entinostat suppresses hepatocellular carcinoma metastasis by upregulating AZGP1 through histone acetylation.

SDC4 is a novel target of the KLF5 transcription factor.

ALDH1L2 induces resistance to chemotherapy in small cell lung cancer by inhibiting ferroptosis.

Dendritic cells targeted CEA tumor antigen through DEC205 in combination with oncolytic reovirus stimulate strong immune response in colorectal cancer model.

Injectable hydrogel induces tumor cell extracellular calcification and bone regeneration to disrupt the osteolytic vicious cycle in bone metastasis.

Targeting the SIN1 mediated TTK/LDHA-H3K18la-GLUT3 axis disrupts metabolic-epigenetic crosstalk and suppresses progression in hyperglycolytic breast cancer.

A multicenter, placebo-controlled clinical trial and preliminary experimental study exploring the efficacy of modified Banxia Xiexin Decoction in the treatment of advanced colorectal cancer.

Introduction: Adenocarcinoma of the colon is the most common malignancy of the Gastrointestinal (GI) tract and a major cause of mortality and morbidity all over the world. Identification of molecular markers associated with carcinogenesis, tumour growth, invasion, and metastasis is essential for developing potential therapeutic management strategies. The Epidermal Growth Factor Receptor (EGFR) is one of the most important genes involved in this carcinogenesis pathway. Aim: To estimate the magnitude of EGFR expression by Immunohistochemistry (IHC), in which the immunospecific tumour cells show membranous positivity for EGFR and are scored accordingly under light microscopy. Materials and Methods: This was an Institution/hospitalbased a single-centred cross-sectional observational study of 29 cases of colon carcinoma. These cases had undergone colectomy operation over 1 year and 6 months from December 2022 to May 2024 at Department of Pathology and Department of General Surgery, Murshidabad Medical College and Hospital, Berhampore, West Bengal, India. Histopathological examination was conducted under a light microscope to confirm the final diagnosis. The scoring system was used to evaluation of EGFR immunostaining. Association between EGFR expression with stage, grade, and tumour budding score was established by the Chi-square test. Values were considered significant at p<0.05 (calculated with the help of Statistical Packages for Social Sciences (SPSS) version 20.0). Results: The study comprised 29 colorectal carcinoma cases, with the highest incidence observed in 9 (31.03%) cases and were in the age group of 41 to 50 years. The male-to-female ratio was 1.4:1. The majority of the tumours were found in the ascending colon 7 (24.14%) cases, followed by the rectum 6 (20.68%) cases, sigmoid colon 6 (20.68%) cases, and caecum 5 (17.24%) cases. Of the 29 cases, 24 were classified as Adenocarcinoma Not Otherwise Specified (NOS) (82.76%), 4 as Mucinous Adenocarcinoma (13.79%), and only 1 as Signet ring cell carcinoma. Among 29 colorectal carcinoma cases, 16 (55.17%) were EGFR positive, and this had a significant association with the stage (p<0.013), grade (p<0.004), and tumour budding score (p<0.033). Conclusion: As the majority of the colorectal carcinoma cases were EGFR positive and had a significant association between the expression of EGFR with high pathological Tumour Node Metastasis (TNM) T stage (T3 and T4), moderately and poorly differentiated carcinoma (G2 and G3 combined) and moderate to high tumour budding score, this finding can be of prognostic significance. Therefore, the EGFR immunohistochemistry biomarker should be included in the standard diagnostic protocol for colorectal cancer, as it helps to screen the EGFR-positive CRC cases, and based on that appropriate chemotherapy can be started.

Synthesis and characterization of new P2X7 receptor antagonists as antitumor agents.