Sites of non-enzymatic crosslinks pentosidine and glucosepane in collagen I of human cortical bone.

Layered COF@Au integrated with pH-responsive DNA triple helix to construct recyclable SERS substrate achieving ultrasensitive detection of ofloxacin

Helicobacter pylori promotes gastric cancer progression through the GATA3-AS1/miR-30c-5p/CTHRC1 axis.

SOX4 is a transcriptional activator for CTHRC1 in lung fibroblast activation.

The era of disruptive technological advancements and the growing demand for sustainable development have brought profound changes to the field of business education, with a specific focus on marketing management education. Although marketing educators must reconsider their teaching practices, it is just as crucial to ensure that they align their education-related research with industry trends as well as expectations of qualification frameworks. Therefore, we intended to uncover missing links in the marketing education scholarship in this regard. Guided by the Triple Helix Model, we conducted three interconnected studies examining marketing education research, industry skill signalling, and qualification frameworks. The studies were designed to triangulate global scholarly trends, lead-market industry expectations, and formalised competency frameworks to identify misalignments and missing links in marketing education scholarship. The analysis identified key themes in extant literature, namely experiential learning and pedagogical practices, instructional strategy and learning impact, curriculum and conceptual foundations, strategic engagement and skill integration, ethical assessment and employability, and digital platforms and learning technologies. Consequently, this study highlights an ongoing gap between research on marketing education and real-world priorities. A research agenda is proposed to better align scholarship of learning and teaching in marketing education. • Marketing education research needs to be aligned with tech trends and policy imperatives. • We identified gaps between marketing education research, industry needs, and qualification framework guidelines. • Three studies analysed marketing education literature, LinkedIn job ads, and qualification/professional frameworks. • An agenda is proposed to align marketing education research with industry and policy goals.

Regulation of the binding and stability of triple helix RNA by enantiomers of ruthenium(II) polypyridyl complexes

Alcohols used as cosolvents with water are known to have a broad range of effects on the native triple helix structure of collagen, from its stabilization (e.g., by glycerol) to its denaturation or its fibrillar precipitation (e.g., by ethanol). We consider gelatin hydrogels as collagenous supramolecular structures and study their resistance to fracture─a localized, stress-induced denaturation process of the collagenous triple-helix cross-links─in an alcoholic environment. Scanning a series of six alcohols, we find that, independently of their contrasted effect on the thermal melting of collagen helices, all of them hinder crack growth. We show that the corresponding increase of fracture energy is attributable to the effect of alcohols on both the differential and direct free energies of solvation, i.e., on their trend to favor the denaturation of the collagenous triple helix or the precipitation of the gelatin network. We find that the latter effect is dominant, all the more that the surface tension of the alcohol is lower. We propose a rationale for this remarkable correlation in the limit of vanishing cosolvent concentrations based on a cavity-filling approach.

Collagen mimetic peptides (CMPs) are useful self-assembling building blocks for creating tunable biomaterials with structure and function dependent on triple helix stability. We demonstrate rapid, UV-activated stabilization of a CMP triple helix via thiomaleimide self-crosslinking controlled by light dose, enabling precise in situ control and stabilization of peptide assemblies.

Collagen imparts structure, viscoelasticity, and bioactivity to the extracellular matrix (ECM) with variance between organs and between healthy and diseased states. Synthetic mimics of collagen-rich tissues remain a need in applications, from biological studies to regenerative medicine, for parsing and controlling these properties. We designed multifunctional collagen mimetic peptides (mfCMPs) that self-assemble into triple helices and fibrils and contain integrin binding motifs: GFOGER, a binding site within intact collagen I, and RGD, a cryptic binding site available within denatured collagen I. These mfCMPs are incorporated into hydrogel-based synthetic ECMs to impart collagen-like hierarchical structures, viscoelasticity, and bioactivity with modularity. We establish innovative methods for imaging the resulting nano- and micro-structures within the hydrogel using super-resolution microscopy. The physically assembled mfCMPs impart tunable, concentration-dependent viscoelasticity within otherwise elastic, covalently cross-linked hydrogels, exhibiting relaxation half times over orders of magnitude and similar to soft tissues. Notably, breast cancer cells encapsulated and cultured in synthetic ECMs with a bioactive fibrillar structure and viscoelastic properties formed large, growing spheroids. Overall, these modular building blocks provide an innovative tool for creating fully synthetic surrogates of collagen-rich microenvironments, aiding both fundamental and translational biological studies and providing a framework to imbue tunable viscoelasticity to synthetic ECMs.

Glycine substitutions in the collagen triple helix cause diverse heritable disorders, but their effects vary with local sequence environment. We tested whether sequence context helps determine the consequences of Gly replacement by combining case-weighted bioinformatic analysis, thermodynamic measurements on collagen model peptides (CMPs), and all-atom molecular dynamics simulations. Case-weighted analysis of pathogenic COL3A1 glycine substitutions identified Pro immediately following the substituted Gly, corresponding to a GP context, as the strongest enriched local feature. To examine this experimentally, we designed CMPs with stabilizing terminal segments flanking native collagen sequence windows containing clinically observed Gly→Ser and Gly→Arg substitutions. Gly→Arg substitutions were generally more destabilizing than Gly→Ser at the same site. However, the strongest effect was sequence-dependent: within the Gly→Ser class, GP-site substitutions caused larger losses of thermal stability and unfolding enthalpy than nonGP substitutions, and some GP-site Gly→Ser mutations were as destabilizing as Gly→Arg substitutions. Molecular dynamics simulations showed that all peptides remained globally triple-helical, but GP-site mutants exhibited greater loss of canonical interchain hydrogen bonds and reduced local backbone accommodation. Thus, the effect of glycine substitution in collagen depends not only on the replacing residue but also on the permissiveness of the surrounding sequence, with Pro-adjacent sites representing especially restrictive local environments.

Sanguisorba officinalis L. polysaccharides: A review of structural characteristics, pharmacological activities, structure-activity relationships and applications.

This paper investigates the drivers behind the institutionalization of engineering ethics at the national level through a comparative study of the United States and the Netherlands. The study examines the historical evolution and current landscape of engineering ethics activities in both countries. By comparing emergence, process, and actor involvement dimensions, commonalities and differences between the two nations’ approaches to the institutionalization of engineering ethics are identified. The analysis reveals context-specific factors shaping engineering ethics policies, including the nature of the profession, technological challenges, and societal attributes. The study identifies common drivers for national engineering ethics development: external influences such as technological crises and societal debates, contextual forces like institutional dynamics and societal attributes, and the triple helix of academia, professional associations, and government relations. The findings may offer useful insights for policymakers and practitioners seeking to enhance engineering ethics.

Herein, we report the development of a cyclic peptide targeting the 3'-end ENE triple helix of the metastatic long non-coding RNA MALAT1. The peptide selectively binds and destabilizes the RNA triple helix, surpasses its linear analog, reduces MALAT1 levels, disrupts spheroid morphogenesis, and induces apoptosis in A549 lung cancer cells.

Purpose This study proposes a conceptual framework for strategic sourcing tailored to Project Management Offices (PMOs) operating in complex Research, Development and Innovation (R&D&I) environments, examining how R&D&I PMOs orchestrate sourcing by identifying key elements and practices that define this strategic role. Design/methodology/approach This study uses a qualitative approach grounded in a three-year ethnographic immersion within a military scientific, technological and innovation institution. Data collection involved participant observation, document analysis and informal interviews, enabling an in-depth examination of sourcing dynamics. Findings The resulting framework integrates three interdependent pillars: five foundational sourcing dimensions; a strategic make-or-buy decision matrix; and management categories aligned with R&D&I operations. The findings show that PMOs coordinate strategic sourcing by integrating internal and external capabilities, thereby enhancing organizational responsiveness in complex innovation ecosystems. Research limitations/implications While the single-case ethnographic study focuses on the aerospace and defense sector, the framework distinguishes between general conceptual pillars and context-specific applications, supporting its conceptual transferability to other highly regulated sectors such as healthcare and pharmaceuticals. The study provides actionable guidance for managing technological uncertainty and power dynamics, while addressing economic, political and teaching implications. Practical implications The proposed framework offers PMO managers a strategic sourcing model suited to complex environments such as the defense sector. It strengthens decision-making by making make-or-buy tradeoffs explicit, documented and comparable across technology acquisition, capability development, outsourcing boundaries and interinstitutional partnerships under confidentiality and intellectual property constraints. The model addresses recurring problems in R&D&I settings, including fragmented criteria, inconsistent rationales and limited traceability, enhancing transparency, governance and alignment with organizational goals. It positions the PMO as a strategic actor in acquisition and technological alliance decisions, offering guidance for institutional adaptation, particularly relevant for public organizations facing budgetary and regulatory constraints. Social implications The societal implications of this study stem from the role of the R&D&I PMO as a catalyst for technological sovereignty and national development. By structuring strategic sourcing in highly complex environments, the proposed model strengthens the national technological and industrial base, reduces dependence on external critical technologies and enhances innovation capacity. The findings show that dual-use R&D projects generate positive spillovers for industry and academia, fostering regional development and national competitiveness. By coordinating government, industry and research institutions through the Triple Helix, the PMO helps ensure that R&D&I investments translate into tangible socioeconomic benefits for society. Originality/value This research addresses the underexplored intersection of strategic sourcing, project management and innovation governance. It goes beyond theoretical abstraction by providing a model for navigating technological uncertainty. It explores how emerging digital technologies (such as Artificial Intelligence and blockchain) can refine decision-making and support the automation of Intellectual Property safeguards.

IntroductionSeafarers face substantial mental health challenges related to isolation, high workloads, and limited access to support, with 77% of surveyed seafarers reporting at least one mental health problem such as sleep disorders, anxiety, or loneliness onboard.MethodsDrawing on the Triple Helix model, through a weighted ratio indicator analysis, this study examines how three stakeholder groups, seafarers, employers, and psychological service providers, perceive seafarers’ mental health challenges and the availability and use of support interventions, based on an online questionnaire completed by 106 respondents (48 seafarers, 44 employers, 14 service providers).ResultsFindings reveal marked misalignment between stakeholders’ understanding of psychological safety, low utilization of available mental health services, and a pronounced imbalance between demand for psychological support and its supply and uptake in employers.ConclusionThe study’s major contribution is to demonstrate, through an integrated supply–demand analysis, where gaps arise between seafarers’ needs, market offerings, and employers’ implementation practices, and to propose evidence-based recommendations for strengthening regulatory frameworks, enhancing stakeholders’ dialog and collaboration, and contextualizing appropriate training and service provision to strengthen psychological safety and mental well-being at sea.

Collagen has been proven to have significant potential applications value in the fields of on medical aesthetics and cosmetics. However, due to its large molecular weight, collagen is difficult to effectively penetrate the skin barrier and reach the designated location to exert the expected activities. Transdermal peptide TD-1 is a short peptide consisting of only 11 amino acids, which can assist proteins such as insulin and cytokines to penetrate the skin barrier and reach the dermis layer. To maximize the transdermal and the efficacy of Collagen III in skincare, the shortest functional fragment that containing a triple helix structure of collagen protein fused with TD-1 were designed and evaluated for its transdermal activity, safety and efficacy. The recombinant protein containing transdermal TD-1 fragment and a core active fragment of human collagen III (TD-1-HrHC) was co-expressed together with specific hydroxylase using the K. phaffii expression system, the products were purified by hydrophobic interaction chromatography (HIC) and ion-exchange chromatography (IEC). The TD-1-HrHC contains about 11.89% hydroxylation modifications that are similar to the natural human collagen III. The transdermal efficiency is 2077% measured by trans-well tests compare to the nature Collagen III isolated from animals. In addition, TD-1-HrHC able to promotes skin damage repairing through regulation series of barrier factors and differentiation factors in the HaCat cell model and promotes the repair of damaged skin and induces the generation of new-born collagen in mouse model. Moreover, it also shown an excellent ability to inhibit protein carbonylation on HaCat cells. This study demonstrates that TD-1-HrHC can effectively cross the skin barrier and has profound ability to inhibit protein carbonylation of skin and promote skin damage repairing.

High-throughput detection is highly significant for food safety, but traditional rapid methods usually target single analytes with a single-signal output, leading to susceptibility to interference and unsatisfactory accuracy. Herein, a high-throughput dual-signal platform was constructed by immobilizing platinum nanoparticle-modified universal triple helix molecular switches on carboxylated 96-well plates. Target recognition induces THMS structural rearrangement and dual-signal generation. Combined with smartphone quantification and MRL judgment, the biosensor showed colorimetric LODs of 0.04, 0.053, and 0.003 ng/mL and fluorescent LODs of 0.053, 0.20, and 0.075 ng/mL for OTA, acetamiprid, and Pb2+, respectively. Its reliable application in corn and wine verified its practicality, providing a promising strategy for accurate food safety POCT.

Triple Helix synergy at the subsidy threshold: Government support, IUR collaboration, and knowledge spillovers in enterprise AI innovation

Mechanical loading is essential for the assembly and maintenance of the articular cartilage extracellular matrix (ECM), whereas alterations in ECM composition profoundly affect cartilage mechanics and function. Type IX collagen is a heterotrimeric fibril-associated collagen with interrupted triple helices (FACIT) that is covalently linked to type II collagen. It restricts lateral fibril growth and mediates interactions with other ECM components. Although type IX collagen expression is mechanosensitive and implicated in cartilage mechanotransduction, its precise functional role is not yet fully understood. This study investigated the combined effects of type IX collagen deficiency and moderate mechanical loading on articular and growth plate cartilage. Twelve-week-old female wild-type (WT) and Col9a1-/- mice were randomly assigned to control (CON) or forced running exercise (EXE) groups (n = 10-12 per group). EXE animals underwent treadmill training for 6 wk (20% incline, 18 m/min, 40 min/day, 5 days/wk). Type IX collagen deficiency resulted in an abnormal growth plate architecture and a reduction of all matrilins and cartilage oligomeric matrix protein (COMP) in articular cartilage. Moderate forced running exercise induced a significant increase (P < 0.05) in cartilage thickness at the lateral femoral condyle and altered ECM composition in Col9a1-/- mice, without evidence of cartilage degeneration. WT mice showed no comparable structural changes. In conclusion, moderate mechanical loading elicits localized, nondegenerative, structural and molecular adaptations in articular cartilage and only modestly modulates the cartilage phenotype associated with type IX collagen deficiency. These findings suggest a limited, yet context-dependent role of type IX collagen in cartilage mechanoadaptation.NEW & NOTEWORTHY Moderate forced running exercise only slightly enhances the cartilage phenotype in aging female type IX collagen knockout mice and induces local, nondegenerative, structural and molecular adaptations in the articular cartilage ECM. Alterations in the cartilage phenotype do not depend on the age of the mice.

Cardiac fibrosis is a key contributor to cardiomyopathy after myocardial infarction (MI). Existing imaging techniques can detect established fibrotic changes; however, they lack sensitivity for ongoing collagen turnover-a dynamic process involving the denaturation of collagen triple helix. Molecular imaging of this process could enhance risk assessment and aid in the development of antifibrotic treatments. This study aimed to evaluate 99mTc-(HE)3-(GPO)9, a radiotracer designed to target denatured collagen, as a biomarker of collagen turnover after MI. This tracer features a polyhistidine-glutamic acid [(HE)3] N-terminal sequence for site-specific radiolabeling linked to a C-terminal-targeting moiety consisting of 9 glycine-proline-hydroxyproline repeats [(GPO)9] via a flexible 3-glycine linker. Methods: MI was induced in mice by ligation of the left anterior descending artery; animals who underwent sham surgery served as controls. At 2 wk after MI, animals underwent myocardial perfusion imaging or contrast-enhanced CT to detect the infarct zone, followed by SPECT/CT imaging with 99mTc-(HE)3-(GPO)9 or a control tracer with scrambled peptide. Tracer uptake was quantified invivo and ex vivo with γ-counting and autoradiography. Different aspects of fibrosis were examined using tissue analysis, along with autoradiography with a matrix metalloproteinase-targeted radiotracer, 99mTc-RYM1, at 3 d, 1 wk, and 2 wk after MI. Tracer binding was also assessed in human cardiac tissue using ex vivo autoradiography. Results: 99mTc-(HE)3-(GPO)9 SPECT/CT revealed significantly higher tracer uptake in the infarct zone of MI mice compared with the remote zone and sham controls (P < 0.0001 for both). Tracer uptake was confirmed by autoradiography, which showed a strong correlation between SPECT and autoradiography (ρ = 0.81, P < 0.05). The control tracer exhibited minimal cardiac uptake, demonstrating the specificity of the 99mTc-(HE)3-(GPO)9 signal. Denatured collagen staining and 99mTc-RYM1 autoradiography showed patterns similar to that shown in ex vivo 99mTc-(HE)3-(GPO)9 autoradiography, whereas the ratio of denatured collagen to procollagen in the infarct zone significantly increased from day 3 to 2 wk after MI. Finally, 99mTc-(HE)3-(GPO)9 demonstrated binding to human fibrotic (but not normal) cardiac tissue. Conclusion: 99mTc-(HE)3-(GPO)9 enabled noninvasive detection of denatured collagen after MI as a marker of collagen remodeling invivo. In combination with other fibrosis imaging tracers, 99mTc-(HE)3-(GPO)9 may provide a comprehensive molecular fingerprint of cardiac fibrosis, advancing personalized management of cardiomyopathy.