The abandonment of traditional forest management and the conversion of lowland woodlands from coppices to high forests may lead to a reduction in plant diversity. We studied long-term changes in semi-natural lowland woodland vegetation in the Czech Republic (Central Europe) by resampling 29 vegetation plots (releves) first sampled in the 1950s. The results indicated a shift to shady plant communities (i.e. loss of heliophilous species and expansion of shade-tolerant species), caused by an increase in the tree and shrub layer canopy due to the expansion of deciduous tree species. At the same time, species richness (alpha diversity) had declined significantly, though we noted no signs of homogenisation in the vegetation cover. Species typical of nutrient-rich habitats and alien and/or invasive species had expanded. We confirmed the shift in vegetation composition, which was probably caused by changes in lowland woodland forest management (decline in coppicing), eutrophication (deposition of atmospheric nitrogen, agricultural runoff) and disturbance effects of game, especially wild boar (Sus scrofa).