Treatment Of Orthopedic Diseases Research Articles

Orthopedic manifestations in children with Prader-Willi syndrome

BackgroundPrader-Willi syndrome (PWS) is a rare genetic disease often associated with bone problems, mainly scoliosis and hip dysplasia (HD). This study aimed to analyze the clinical characteristics of orthopedic deformities in patients with PWS.MethodsA retrospective study was conducted on 175 patients up to March 2023. The Cobb angle(CA) of the spine, the alpha angle of the hip joint, and the acetabular index (AI) were measured. This study aimed to evaluate the relationship between demographic parameters and bone deformities.ResultsScoliosis was found in 66 patients (43.7%), including 52 (78.8%) with mild scoliosis, 10 (15.2%) with moderate scoliosis, and 4 (6.1%) with severe scoliosis. Only seven patients received orthopedic treatment (10.6%). The median age of scoliosis was 4.5 years old, and the prevalence of scoliosis increased rapidly at the age of 5 years and adolescence. The mean CA in this study increased gradually with age. HD was found in 47 patients (38.2%), and 6 patients received orthopedic treatment (12.7%). The median age at HD was 1.8 years old. The mean AI of the study population decreased with age. The prevalence of HD treated with recombinant human growth hormone (rhGH) was low. No significant differences were observed in sex, genotype, body mass index (BMI), obesity rate, or onset of scoliosis and HD.ConclusionThe prevalence of scoliosis and HD was higher in patients with PWS. The onset age and developmental trends of the different skeletal malformations were different. Early diagnosis and treatment are important for the prognosis and treatment of orthopedic diseases in patients with PWS.

Polydopamine-Based Biomaterials in Orthopedic Therapeutics: Properties, Applications, and Future Perspectives.

Polydopamine is a versatile and modifiable polymer, known for its excellent biocompatibility and adhesiveness. It can also be engineered into a variety of nanoparticles and biomaterials for drug delivery, functional modification, making it an excellent choice to enhance the prevention and treatment of orthopedic diseases. Currently, the application of polydopamine biomaterials in orthopedic disease prevention and treatment is in its early stages, despite some initial achievements. This article aims to review these applications to encourage further development of polydopamine for orthopedic therapeutic needs. We detail the properties of polydopamine and its biomaterial types, highlighting its superior performance in functional modification on nanoparticles and materials. Additionally, we also explore the challenges and future prospects in developing optimal polydopamine biomaterials for clinical use in orthopedic disease prevention and treatment.

Аутологичная кондиционированная сыворотка в лечении пациентов с ортопедическими заболеваниями: обзор литературы

Treatment of orthopedic diseases is a pressing problem. Autologous conditioned serum is used in the treatment of osteoarthritis and exhibits a pronounced analgesic effect and improves joint function without significant side effects, even in cases resistant to other treatments. Autologous conditioned serum is also successfully used for meniscus pathology, tendon diseases, and osteochondrosis. Treatment with autologous conditioned serum is not only an effective method, but is also economically affordable.

Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway.

Total flavonoids of Rhizoma drynariae (TFRD) is broadly used in the treatment of orthopedic diseases. Nevertheless, the effects and underlying mechanism of TFRD on tendon-bone healing after anterior cruciate ligament reconstruction (ACLR) remain unclear. The ACLR mouse model was established. Hematoxylin and Eosin (HE) staining was used for histological analysis of tendon-bone healing. Western blot was utilized to detect the levels of osteogenic related factors (ALP, OCN, RUNX2). The viability and alkaline phosphatase (ALP) activity of bone mesenchymal stem cells (BMSCs) were determined by Cell Counting Kit-8 (CCK-8) and ALP assays. The interaction of estrogen related receptor alpha (ESRRA), estrogen related receptor beta (ESRRB), and golgi-localized γ-ear containing ADP ribosylation factor-binding protein 1 (Gga1) was detected by luciferase reporter assays. The levels of important proteins on the TGF-β/MAPK pathway were measured by western blot. TFRD improved tendon-bone healing, restored biomechanics of ACLR mice and activated the TGF-β/MAPK pathway. TFRD treatment also enhanced the viability and osteogenic differentiation of BMSCs in vitro. Then, we demonstrated that TFRD targeted ESRRA and ESRRB to transcriptionally activate Gga1 expression. Knockdown of ESRRA, ESRRB, or Gga1 suppressed the viability and osteogenic differentiation of TFRD-induced BMSCs, which was revealed to be restored by Gga1 overexpression. The overexpression of ESRRA, ESRRB, or Gga1 was demonstrated to promote the BMSC viability and osteogenic differentiation. TGF-β1 treatment can reverse the impact of Gga1 inhibition on osteogenic differentiation in TFRD-induced BMSCs. TFRD improves tendon-bone healing in ACLR mouse models and facilitates the osteogenic differentiation of BMSCs through the ERR1/2-Gga1-TGF-β/MAPK pathway, which might deepen our understanding of the underlying mechanism of TFRD in tendon-bone healing.

Early Continuous Passive Motion and Needle-knife Therapy Alleviate Knee Motor Dysfunction Effects After Internal Fixation of TPFs

Posttraumatic osteoarthritis (PTOA) is a common complication secondary to tibial plateau fractures (TPFs) after internal fixation. Early continuous passive motion (CPM) and needle-knife therapy are both alternative medicine treatments that have been beneficial to PTOA. CPM and needle-knife therapy are commonly applied separately in the treatment of orthopedic diseases and soft tissue injuries, but they both have limitations. In this study, we combined CPM and needleknife therapy to observe its efficacy on PTOA caused by postoperative TPFs and its possible mechanisms. A rabbit model of TPFs after internal fixation was established by surgical method. Rabbits were randomly divided into five groups: Normal control (NC) group, Sedentary (SED) group, CPM group, Needle-knife group, CPM + Needle-knife group. Range of motion (ROM), knee diameter and medial cartilaginous surface of the tibial plateau were observed after 4 weeks of treatment. Levels of inflammatory cytokines in the supernatants from knee joint fluid, including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, were determined by enzyme-linked immunosorbent assay (ELISA). The gross view of ligament tissues was detected by immunosorbent assay (ELISA). The gross view of ligament tissues was detected by histomorphology, including Hematoxylin and Eosin (H&E) staining and Masson's trichrome staining. Other than that, the protein expression of matrix metalloproteinase (MMP)-13 and tissue inhibitors of metalloproteinase (TIMP)-1 were evaluated by immunohistochemical staining, respectively. Finally, the apoptosis of ligament cells was assessed through DAPI and TUNEL assay. Our data showed that ROM was reduced while knee diameter was increased in the SED group, but they were relieved by CPM and needle-knife—either singly or in combination treatment. CPM and needle-knife therapy also improved the effect of TPFs on other parameters, including the medial cartilaginous surface of the tibial plateau improvement, MMP-13 and TIMP-1 expression in the medial collateral ligament (MCL), and lateral collateral ligament (LCL), knee joint fluid IL-1β, IL-6, and TNF-α levels, and condition of ligament cells apoptosis. Our findings suggested that early CPM plus needle-knife therapy might be a useful and promising therapeutic method for PTOA.

Layered Double Hydroxides: A Novel Promising 2D Nanomaterial for Bone Diseases Treatment.

Bone diseases including bone defects, bone infections, osteoarthritis, and bone tumors seriously affect life quality of the patient and bring serious economic burdens to social health management, for which the current clinical treatments bear dissatisfactory therapeutic effects. Biomaterial-based strategies have been widely applied in the treatment of orthopedic diseases but are still plagued by deficient bioreactivity. With the development of nanotechnology, layered double hydroxides (LDHs) with adjustable metal ion composition and alterable interlayer structure possessing charming physicochemical characteristics, versatile bioactive properties, and excellent drug loading and delivery capabilities arise widespread attention and have achieved considerable achievements for bone disease treatment in the last decade. However, to the authors' best knowledge, no review has comprehensively summarized the advances of LDHs in treating bone disease so far. Herein, the advantages of LDHs for orthopedic disorders treatment are outlined and the corresponding state-of-the-art achievements are summarized for the first time. The potential of LDHs-based nanocomposites for extended therapeutics for bone diseases is highlighted and perspectives for LDHs-based scaffold design are proposed for facilitated clinical translation.

DESIGNING CLOTHING FOR CHILDREN WITH ORTHOPEDIC DEFECTS

This article examines the technology of production of special clothing designed for children during the treatment of orthopedic diseases, in particular such a disease as hip dislocation, analyzes the existing in our country types of clothing for children with orthopedic defects, as well as their foreign analogues. The physical and mechanical parameters of fabrics for the production of special children’s clothing were determined, and recommendations for basic and additional types of materials for the clothing of children with orthopedic defects were developed based on the results of the research. A total of 180 children aged from 0 to 11 months in the city of Tashkent and Jizzax province were examined. Among the fixation bandages for the hip joint the widely used Freik splint in 5 sizes was recommended. Taking into account natural and climatic conditions of Uzbekistan and on the basis of the results of studies on the design of special clothing for children with orthopedic defects, a set of special clothing made of cotton fabric of domestic production was formed.

Novel Role of Biomedical Sensors and CT/MRI Scanning Image Segmentation Algorithms in Orthopedic Diseases

We aimed to evaluate the efficacy of medical image segmentation algorithms in conjunction with biomedical sensors for the diagnosis and treatment of orthopedic diseases. The two-dimensional image data of orthopedic patients were obtained by using CT/MRI scanning along with the biomedical sensors. Patients are divided into: control group (n = 140 cases) and experimental group (106 cases). The control group has received the traditional orthopedic surgery analysis method, while the experimental group has adopted the medical image segmentation, biomedical sensors and MRI scanning for the treatment/surgery of orthopedic patients. There is a apparently different level of performance between two groups (P <0.05). The analgesic and sedative effect of the experimental group is observed at 2 h, 6 h, and after 12 h respectively and it is found that the experimental group exhibits better results with statistical significance (P < 0.05). The experimental group has better rates of fracture, fracture nonunion, osteoporosis, and femoral head necrosis, and a substantial difference in various disease classifications is observed between two groups (P <0.05). There is a considerable gap between two groups in the rate of subsequent operations. The experimental group has much higher rate of subsequent operations than the control group (P <0.05). The proposed innovative non-invasive medical treatment methods can not only enhance the accuracy of orthopedic surgeries.

Relationship between Gut Microbiota and Bone Health.

Gut Microbiota (GM) are microorganisms that live in the host gastrointestinal tract, and their abundance varies throughout the host's life. With the development of sequencing technology, the role of GM in various diseases has been increasingly elucidated. Unlike earlier studies on orthopedic diseases, this review elucidates the correlation between GM health and bone health and discusses the potential mechanism of GM effects on host metabolism, inflammation, and ability to induce or aggravate some common orthopedic diseases, such as osteoarthritis, osteoporosis, rheumatoid arthritis, etc. Finally, the prospective methods of GM manipulation and evaluation of potential GM-targeting strategies in the diagnosis and treatment of orthopedic diseases are reviewed.

Molecular Mechanism of Ferroptosis in Orthopedic Diseases.

Ferroptosis is a new iron-dependent programmed cell death process that is directly mediated by the accumulation of lipid peroxides and reactive oxygen species. Numerous studies have shown that ferroptosis is important in regulating the occurrence and development of bone-related diseases, but the underlying mechanisms are not completely clear. Herein, we review the progress of the mechanism of ferroptosis in bone marrow injury, osteoporosis, osteoarthritis, and osteosarcoma and attempt to deeply understand the regulatory targets of ferroptosis, which will open up a new way for the prevention and treatment of orthopedic diseases.

Application of Huangqi Guizhi Wuwu Decoction in Orthopaedics and Traumatology

AbstractThe Huangqi Guizhi Wuwu Decoction(HQGZWWT) is composed of Huangqi (Astragali Radix), Shaoyao (Paeoniae Radix Alba), Guizhi (Cinnamomi Ramulus), Shengjiang (Zingiberis Rhizoma Recens), and Dazao (Jujubae Fructus) which has various pharmacological anti-inflammatory, analgesic, antioxidant, antitumor, lipid-lowering, and immunity-regulating activities. It has certain advantages in the treatment of orthopaedic diseases, such as cervical spondylosis, scapulohumeral periarthritis, lumbar disc herniation, knee osteoarthritis, rheumatoid arthritis, myofascial pain syndrome, etc., with outstanding clinical efficacy, few adverse reactions, and high patient compliance. However, modern pharmacological researches on the whole prescription of HQGZWWT are insufficient and the therapeutic targets are not clear which needs further exploration. Besides, this prescription cannot treat all orthopaedic diseases, so we should adhere to the thinking of four diagnostics in traditional Chinese medicine (TCM), select prescriptions based on syndrome differentiation, closely follow the pathogenesis, innovate and expand its scope of application, reflect the application advantages of this prescription in orthopaedics and traumatology and improve the total clinical efficiency.

Effects of dynamic mechanical stimulations on the regeneration of in vitro and in vivo cartilage tissue based on silk fibroin scaffold

Due to the lack of blood and nutrition supply, the repairing of cartilage defect has become a challenging topic in clinical treatment. Herein, a unique porous scaffold based on regenerated silk fibroin (RSF) was fabricated by using freeze-drying after enzymatical crosslinking. Results showed that the pore size, mechanical properties and cytocompatibility of the scaffold were all significantly improved by proper combination of bacterial cellulose nanofiber ribbon (BCNR), thus much appropriate for cartilage regeneration. Based on this RSF/BCNR composite scaffold , the effects of stair-stepping shaped dynamic hydrostatic pressure (DHP) on the construction of tissue engineered cartilage in vitro and dynamic loading microenvironment on the regeneration of cartilage defect in vivo have been comprehensively investigated for the first time. Compared with traditional static culture, DHP has obviously enhancing chondrocyte growth and cartilage specific matrix deposition. Compared with the unloading microenvironment in vivo , corresponding dynamic loading microenvironment significantly improved cartilage regeneration, as it presented apparent cartilage lacuna like structure and thicker newly formed cartilage tissue. The insights of in vitro and in vivo dynamic mechanical stimulations enhancing effects on cartilage regeneration based on the RSF/BCNR composite scaffold revealed in this study could provide valuable guidance for the construction of optimal tissue engineered cartilage and the clinical prevention and treatment of orthopedic diseases. • A unique RSF/BCNR composite scaffold with improved pore size, mechanical strength and cytocompatibility was fabricated. • Effects of dynamic mechanical stimulations on the in vitro and in vivo cartilage regeneration have been comprehensively investigated. • Dynamic mechanical stimulations have significantly improved cartilage regeneration based on the RSF/BCNR scaffold.

Efficacy of total flavonoids of Rhizoma drynariae on the blood vessels and the bone graft in the induced membrane

BackgroundTotal flavonoids of Rhizoma drynariae (TFRD), a Chinese medicine, is widely used in the treatment of orthopedic diseases. However, there are few basic and clinical studies on the effect of TFRD on induced membrane technique (Masquelet technique). PurposeThis trial is to explore effects of TFRD on vascularization of the induced membrane, and mineralization of the bone graft in rats with femoral bone defects. Study design and methodsForty-eight Sprague-Dawley rats were randomly divided into high dose group (H-TFRD), medium dose group (M-TFRD), low dose group (L-TFRD) and control group (control). The segmental bone defects were established with 12 rats in per group. The polymethyl methacrylate (PMMA) spacer was implanted into the femoral bone defect of rats in the first-stage surgery. About 4 weeks after first-stage surgery, induced membranes of 6 rats in each group were selected. The blood vessels and angiogenesis-related factors in the induced membrane were analyzed by hematoxylin-eosin (HE) and masson staining, western blot, qPCR and immunohistostaining. The remaining rats in per group underwent second-stage surgery (bone grafting). Twelve weeks after the bone grafting, the bone tissues was examined by X-ray, micro-computed tomography (Micro-CT), HE staining and enzyme-linked immunosorbent assay (ELISA) to evaluate the growth of the bone graft. Meanwhile, the TFRD-containing serum was collected from rats to culture osteoblasts in vitro. Cell Counting Kit-8 (CCK-8) method, Alizarin Red S (ARS) staining, western blot and immunofluorescence were used to detect effects of TFRD on the osteoblasts’ proliferation and BMP-SMAD signaling pathway. ResultsCompared with the L-TFRD and control groups, the number of blood vessels and the expression of angiogenesis-related factors (VEGF, TGF-β1, BMP-2, PDGF-BB and CD31) were higher in the H-TFRD and M-TFRD groups. The Lane-Sandhu X-ray score, bone mass and growth rate of the bone graft in the H-TFRD and M-TFRD groups were significantly better than those in the L-TFRD and control groups. In addition, medium and high doses of TFRD significantly increased the expression of BMP-SMAD pathway proteins (BMP-2, SMAD1, SMAD4, SMAD5 and RUNX2) in rat serum and bone graft. In vitro, after osteoblasts were intervened with TFRD-containing serum from the H-TFRD and M-TFRD groups, the cell viability, the number of mineralized nodules and the phosphorylation of BMP-SMAD pathway proteins were markedly increased. ConclusionTFRD could promote the formation of blood vessels and the expression of angiogenesis-related factors during the formation of the induced membrane. During the growing period of bone graft, it could facilitate the growth and mineralization of bone graft in a dose-dependent manner, which is partly related to the activation and phosphorylation of BMP-SMAD signaling pathway.

Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis.

Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo, which is associated with the pathogenesis and treatment of orthopedic diseases. Therefore, it is of great significance to investigate the effects of corresponding compound stimulation on cell behaviors. Herein, a fluid shear stress (FSS) plus ultrasound stimulation platform suitable for cell studies based on a microfluidic chip was constructed and bone marrow mesenchymal stem cell (BMSC) was chosen as a model cell. The proliferation and osteogenesis of BMSCs under the compound stimulation of FSS plus ultrasound in growth medium without any soluble induction factors were firstly investigated. Single FSS stimulation and static culture conditions were also examined. Results illustrated that suitable single FSS stimulation (about 0.06 dyn/cm2) could significantly enhance cell proliferation and osteogenesis simultaneously when compared to the static control, while greater FSS mitigated or even restricted these enhancing effects. Interestingly, ultrasound stimulation combined with this suitable FSS stimulation further accelerated cell proliferation as the intensity of ultrasound increasing. As for the osteogenesis under compound stimulation, it was relatively restricted under lower ultrasound intensity (about 0.075 W/cm2), while promoted when the intensity became higher (about 1.75 W/cm2). This study suggests that both the cell proliferation and osteogenesis are very responsive to the magnitudes of FSS and ultrasound stimulations and can be both significantly enhanced by proper combination strategies. Moreover, these findings will provide valuable references for the construction of effective cell bioreactors and also the treatment of orthopedic diseases.

The advances of topology optimization techniques in orthopedic implants: A review.

Metal implants are widely used in the treatment of orthopedic diseases. However, owing to the mismatched elastic modulus of the bone and implants, stress shielding often occurs clinically which can result in failure of the implant or fractures around the implant. Topology optimization (TO) is a technique that can provide more efficient material distribution according to the objective function under the special load and boundary conditions. Several researchers have paid close attention to TO for optimal design of orthopedic implants. Thanks to the development of additive manufacturing (AM), the complex structure of the TO design can be fabricated. This article mainly focuses on the current stage of TO technique with respect to the global layout and hierarchical structure in orthopedic implants. In each aspect, diverse implants in different orthopedic fields related to TO design are discussed. The characteristics of implants, methods of TO, validation methods of the newly designed implants, and limitations of current research have been summarized. The review concludes with future challenges and directions for research. Wang TO design of global layout and local structure of implants in diverse fields of orthopedic.

Research trends of platelet-rich plasma application in orthopaedics from 2002 to 2020: a bibliometric analysis.

Platelet-rich plasma (PRP) has been widely used to treat various orthopaedic diseases, and there are a large number of studies on the matter. However, there have been no bibliometric reports on the publications in this field. Bibliometrics is an excellent method to systematically evaluate the current research status of a specific field. In this study, CiteSpace 5.7.R2 software was used to analyse the status, hotspots, and frontiers of PRP in the treatment of orthopaedic diseases in the past 19years. All articles about the application of PRP in orthopaedics from 2002 to 2020 were searched from the Web of Science Core Collection. The author, country, institution, journal, cited journal, and keywords were retrieved, and the bibliometric analysis was done in CiteSpace 5.7.R2. A total of 321 articles were retrieved. The analysis showed that the number of publications increased in the past 19years, and the most productive author in this field was Brian J. Cole. The most productive country and the most productive institution were the US and the Hospital for Special Surgery in New York, respectively. The journals with the highest output and the highest citation frequency were the Journal of Orthopaedic Research and the American Journal of Sports Medicine, respectively. From the keyword analysis, we observed that the primary research hotspots were the study of the PRP composition, growth factors, and the combination of PRP and mesenchymal stem cells. The frontiers of PRP were in vivo experiments, the treatment of rotator cuff and cartilage injury, and the comparison of clinical efficacy between PRP and hyaluronic acid. The efficacy of PRP in treating orthopaedic diseases remains controversial. There are still some problems with the experimental methods, such as insufficient sample size, low level of evidence, confusion of system naming, lack of standardisation of preparation methods and application programs, and lack of in-depth basic scientific research. The research status shown by co-occurrence and cluster maps and the analysis of hotspots and frontiers may help solve present problems and explore new directions.

Preparation and Characterization of Sustained-Release Naringin Coating on Magnesium Surface

Given the three-dimensional multi-level structure of natural bone and the multi-factor time-shifting effect in the healing process after bone trauma, there are plans to introduce drug-controlled release systems into the treatment of orthopedic diseases. To achieve multi-level loading and controlled release of biologically active substances, it is necessary to create synergistic behavior between biological factors, thereby improving the bone regeneration ability of artificial bone replacement materials. A naringin-loaded (NG) coating was prepared, compared with ultrasonic micro-arc oxidation (UMAO). The coating was characterized by X-ray diffraction, infrared spectroscopy, and scanning electron microscopy. The corrosion resistance of the coating was studied through the wetting angle and polarization curve. The high-performance liquid chromatography method was used to test the release of the drug. It can be seen from the experimental results that the NG coating has a larger wetting angle and better corrosion resistance. In addition, the NG coating produces more apatite substances and has good biological activity. The NG coatings can stimulate the natural bone regeneration and repair process by releasing drugs during the process, which can effectively promote bone regeneration and repair after implantation in the body.

Molecular basis and therapeutic potential of myostatin on bone formation and metabolism in orthopedic disease.

Myostatin, a member of the transforming growth factor-β (TGF-β) superfamily, is a key autocrine/paracrine inhibitor of skeletal muscle growth. Recently, researchers have postulated that myostatin is a negative regulator of bone formation and metabolism. Reportedly, myostatin is highly expressed in the fracture area, affecting the endochondral ossification process during the early stages of fracture healing. Furthermore, myostatin is highly expressed in the synovium of patients with rheumatoid arthritis (RA) and is an effective therapeutic target for interfering with osteoclast formation and joint destruction in RA. Thus, myostatin is a potent anti-osteogenic factor and a direct modulator of osteoclast differentiation. Evaluation of the molecular pathway revealed that myostatin can activate SMAD and mitogen-activated protein kinase signaling pathways, inhibiting the Wnt/β-catenin pathway to synergistically regulate muscle and bone growth and metabolism. In summary, inhibition of myostatin or the myostatin signaling pathway has therapeutic potential in the treatment of orthopedic diseases. This review focused on the effects of myostatin on bone formation and metabolism and discussed the potential therapeutic effects of inhibiting myostatin and its pathways in related orthopedic diseases.

Principles of diagnosis and treatment of orthopaedic diseases during coronavirus disease 2019 (COVID-19)

The epidemic of corona virus disease 2019 (COVID-19) is still ongoing, and infections among health care workers are not uncommon How to complete the diagnosis and treatment of orthopaedic diseases and prevent the spread of the epidemic is a great challenge for orthopaedic surgeons On the basis of exposure history and medical conditions of orthopaedic patients, the hierarchical control is very important for reduction of the exposure for the medical staff and patients In order to ensure medical safety and reduce the consumption of materials for the epidemic prevention, we classified the hospitalized orthopaedic patients into three classes and six levels, to provide effective references for all levels of hospitals in orthopaedic clinical work Copyright © 2020 by the Chinese Medical Association

ODNA: a manually curated database of noncoding RNAs associated with orthopedics.

Orthopedics is an important branch of medicine concerned with disorders of the bones, joints, spinal cord, peripheral nerves, muscles, connective tissue and cartilage (1). Orthopedic disorders, such as back pain and arthritis, have high incidence rates and affect the quality of life of most people at some point during their lifetime (2,3). In addition, many disorders of other body systems, including the vascular, nervous, immune and metabolic systems, may also affect the musculoskeletal system and complicate the diagnosis and treatment of orthopedic diseases (4–6). The causes of diseases involving the musculoskeletal system have not been well elucidated. Currently, multiple mechanisms, including proteomic, transcriptomic, genomic, epigenetic regulatory and environmental mechanisms, have been shown to be involved in the development of orthopedic disorders (7,8). Over the past decade, studies have revealed new classes of RNA molecules, including microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), long noncoding RNAs (lncRNAs), PIWI-interacting RNAs (piRNAs) and circular RNAs (circRNAs). These noncoding RNAs (ncRNAs) not only regulate a variety of cell functions in the development of and physical processes involving skeletal muscle but also contribute to a number of orthopedic-related disorders (9–11). For example, miRNAs are believed to regulate the differentiation of osteoblasts and osteoclasts through upregulation or downregulation or sponging (12). LncRNAs have been identified as critical molecules that regulate bone and cartilage degeneration, promote bone metastases and repair spinal cord injuries. It has also been reported that the abnormal expression of lncRNAs leads to the malformation of bones and homeotic transformation (13). Differentially expressed circRNAs may play important roles in the differentiation and proliferation of stem cells. The abnormal expression of circRNAs is also involved in arthritis, osteoporosis and hereditary bone diseases (14–16). In recent years, studies on the associations between ncRNAs and orthopedic disorders have rapidly accumulated. However, the data about these associations have remained scattered. To facilitate investigations into the associations between orthopedic disorders and ncRNAs, we constructed a public database, the Orthopedic Diseases ncRNAome Atlas (ODNA), which contains manually curated data on orthopedic disease-associated, experimentally validated ncRNAs.