Event Abstract Back to Event Application of powdered reconstituteable liquid skin substitute for cell therapy Aziz Ghahary1, Ryan Hartwell1, Ruhangiz T. Kilani1 and Reza B. Jalili1 1 University of British Columbia, Surgery/Plastic surgery, Canada Introduction: There is now a new trend in using cell therapy of using specific types of either stem or normal cells to compensate the function of tissue / organ in question or repair damaged tissues. For example, it has been shown that injected nonuse / human insulin producing islet improves the level of glucose in treated animal model. It is also shown that intradermal injections of allogeneic fibroblasts have therapeutic potential for treatment of recessive dystrophic epidermolysis bullosa. Considered the fact that with the exception of immune cells, all other cells in the body are embedded within some kind of scaffold, and it is very challenging to inject cell populated solid scaffold, here we have hypothesized that a liquid scaffold can be developed within which not only cells can be delivered into the organ of interest but also serve as viable scaffold for cell viability and functionality. Methods: Three scaffolds were made of 1) collagen:GAG, (2) collagen:GAG crosslinked with 0.02%w/w glutaraldehyde, (3) collagen:GAG crosslinked with 0.02% glutaraldehyde and containing a crosslinked PVA hydrogel and their gelling rates, toxicity, contracture, cell proliferation, tensile strength and cellular architecture were measured and compared. The liquid scaffold was then used to deliver insulin-producing islets into kidney capsules of diabetic mouse model as well as in delayed wound healing model. Results: In situ gelling scaffolds, containing hydrogels, were non-toxic and exhibited significantly faster fibril formation than controls (p<0.05). Hydrogel scaffolds demonstrated a greater mechanical strength, as well as resistance to contracture and degradation (p<0.05). Wounds treated with composite gel scaffolds showed a significantly shorten inflammatory phase of wound healing and promoted an early epithelialization. Further, islets /scaffold delivered into a kidney capsule of diabetic mouse normalized the blood glucose level due to a greater islet viability and functionality. Conclusions: Collectively our data suggest that our powdered reconstituteable liquid scaffold is not only a promising method to provide rapid, integrative wound coverage but also as a new strategy in cell/scaffold therapy. NSERC and CIHR Keywords: Extracellular Matrix, 3D scaffold, Burn wound, Cell functionality Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Biomaterials for therapeutic delivery Citation: Ghahary A, Hartwell R, Kilani RT and Jalili RB (2016). Application of powdered reconstituteable liquid skin substitute for cell therapy. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00227 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Aziz Ghahary Ryan Hartwell Ruhangiz T Kilani Reza B Jalili Google Aziz Ghahary Ryan Hartwell Ruhangiz T Kilani Reza B Jalili Google Scholar Aziz Ghahary Ryan Hartwell Ruhangiz T Kilani Reza B Jalili PubMed Aziz Ghahary Ryan Hartwell Ruhangiz T Kilani Reza B Jalili Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.